Altered brain structural covariance networks of the thalamic subfields in right chronic capsular stroke

Jun Guo¹Hongchuan Zhang²Jingchun Liu³Caihong Wang⁴Chen Cao¹Jingliang Cheng⁵Chunshui Yu^3,6Wen Qin^3,6*

• ¹Tianjin Huanhu Hospital, Tianjin, China
• ²Yijishan Hospital of Wannan Medical College, Wuhu, China
• ³Department of Radiology and Tianjin Key Laboratory of Functional Imaging, 7 Tianjin Medical University General Hospital, Tianjin, China
• ⁴The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China
• ⁵The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
• ⁶Tianjin Medical University General Hospital, Tianjin, China

Background: The thalamus, along with its component nuclei, possesses extensive connections with various brain regions and is engaged in diverse functions. However, it is unknown whether the gray matter volume (GMV) covariance networks of thalamic subfields are selectively affected in chronic capsular stroke. Methods: We recruited 45 patients with chronic right capsular strokes (CS) and 93 normal controls (NC) from three centers. The thalamus was segmented into 25 subfields using FreeSurfer (v7.1.1). A general linear model was applied to investigate intergroup differences in the GMV covariance network of each thalamic subfield with each voxel of the entire brain between CS and NC, correcting for confounders such as age, gender, total intracranial volume (TIV), and scanners (voxel-wise P<0.001, cluster-wise FWE corrected P<0.05). Results: Our findings revealed that all 25 ipsilesional thalamic subfields in CS were atrophied (P<0.05, FDR correction). Among these, 16 ipsilesional thalamic subfields (including AV, LD, LP, VLa, VLp, VPL, VM, CeM, CL, MDm, LGN, PuM, PuI, CM, Pf, and Pt) exhibited significantly subfield-specific increased GMV covariance connectivity with the anterior orbital gyrus, superior occipital gyrus, calcarine, anterior cingulate cortex, precentral gyrus, and other regions. Additionally, although none of the contralesional thalamic subfields demonstrated regional GMV changes, 3/25 showed subfield-specific increased GMV covariance connectivity with the ipsilesional anterior orbital gyrus and subcortex. Conclusions: The GMV covariance networks of thalamic subfields are selectively involved in patients with chronic capsular stroke, which affect not only the ipsilesional thalamic subfields but also the contralesional ones.