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CORRECTION article

Front. Neurosci., 17 November 2025

Sec. Neurodegeneration

Volume 19 - 2025 | https://doi.org/10.3389/fnins.2025.1657393

Correction: miR-124 alleviates ischemic stroke-induced neuronal death by targeting DAPK1 in mice

This article is a correction to:

  1. miR-124 Alleviates Ischemic Stroke-Induced Neuronal Death by Targeting DAPK1 in Mice

    1. Read original article
Yan Shi&#x;Yan Shi1†Tian Tian,&#x;Tian Tian2,3†Er-Li CaiEr-Li Cai4Can YangCan Yang5Xin Yang, Xin Yang2,3*
  • 1Faculty of Laboratory Medicine, School of Medicine, Hunan Normal University, Changsha, China
  • 2The Brain Cognition and Brain Disease Institute, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
  • 3Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Guangdong Key Lab of Brain Connectomics, Shenzhen, China
  • 4Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
  • 5Department of Emergency Surgery, Hubei Provincial Hospital of Integrated Chinese and Western Medicine, Wuhan, China

A Correction on
miR-124 alleviates ischemic stroke-induced neuronal death by targeting DAPK1 in mice

by Shi, Y., Tian, T., Cai, E.-L., Yang, C., and Yang, X. (2021). Front. Neurosci. 15:649982. doi: 10.3389/fnins.2021.649982

There was a mistake in Figure 2 as published. Incorrect images were used in Figure 2A. The corrected Figure 2 appears below.

Figure 2
Western blot analysis and bar graphs showing the expression levels of DAPK1 in various conditions. Panel A shows protein bands for DAPK1 and DM1A in Sham, PIT-stroke, Control, and OGD conditions. Panels B and C present bar graphs illustrating increased DAPK1 expression in PIT-stroke and OGD conditions, respectively. Panel D displays relative expression levels of miR-124 and DAPK1, with significant increases in oe-miR-124. Panel E shows protein bands for various proteins in oe-control and oe-miR-124 conditions. Panel F provides bar graphs of relative intensities for multiple proteins, with significant reductions in oe-miR-124.

Figure 2. DAPK1 was identified as a target gene of miR-124. DAPK1 levels were measured by western blotting (A) and quantitatively analyzed (B, C). Data are presented as mean ± SD (n = 4/group). **p < 0.01, ***p < 0.001 vs. Con or Sham group. N2a cells were transfected with miR-124 (oe-miR-124) or control-treated (oe-control) cells. Levels of miR-124 and DAPK1 mRNA were measured by qRT-PCR (D). Levels of DAPK1, caspase-3, cleaved caspase-3, p35/25, ERK1/2, and p-ERK1/2 were measured by western blotting (E) and quantitatively analyzed (F). Data are presented as mean ± SD (n = 4/group). **p < 0.01, ***p < 0.001 vs. oe-control group.

There was a mistake in Figure 4 as published. Incorrect images were used in Figure 4A. The corrected Figure 4 appears below.

Figure 4
Image composed of multiple panels presenting scientific data. Panel A shows four microscopy images labeled Con, OGD, Anta-miR-124, and OGD+Ago-miR-124, depicting cell staining with varying intensities. Panel B is a bar graph displaying relative TUNEL positive cells across different conditions, with statistical significance marked by asterisks. Panel C presents brain slices under different treatments: Sham, PIT-stroke, Anta-miR-124, and PIT-stroke+Ago-miR-124. Panel D features Western blot images for DAPK1, Caspase3, Cleaved-Caspase3, and DM1A. Panel E shows a bar graph of relative protein intensities. Panel F is a line graph of neurological scores over time. Panel G presents a line graph of retention time in seconds. Statistical significance is indicated in panels E, F, and G.

Figure 4. miR-124 protected against PIT-stroke damage. (A) TUNEL staining was performed in primary cultured neurons after OGD treatment with different miR-124 levels (scale bar = 50 μm) and quantified (B). Data are presented as mean ± SD (n = 4/group). **p < 0.01 vs. Con, ##p < 0.01 vs. OGD group. (C) Representative images of TTC staining depicting Ago-miR-124-induced protection against PIT-stroke damage. Levels of DAPK1, caspase-3, and cleaved caspase-3 were measured by western blotting (D) and quantitatively analyzed (E). Neurological scores (F) and performance on the rotarod test (G) were tested on day 1, day 3, and day 7 after PIT-stroke among different groups. Data are presented as mean ± SD (n = 6/group). **p < 0.01, ***p < 0.001 vs. Sham, #p < 0.05, ##p < 0.01 vs. PIT-stroke group.

The original version of this article has been updated.

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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: stroke, cell death, miR-124, DAPK1, neuron

Citation: Shi Y, Tian T, Cai E-L, Yang C and Yang X (2025) Correction: miR-124 alleviates ischemic stroke-induced neuronal death by targeting DAPK1 in mice. Front. Neurosci. 19:1657393. doi: 10.3389/fnins.2025.1657393

Received: 01 July 2025; Accepted: 31 October 2025;
Published: 17 November 2025.

Edited and reviewed by: Mark P. Burns, Georgetown University, United States

Copyright © 2025 Shi, Tian, Cai, Yang and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Xin Yang, eWFuZ3hpbkBzaWF0LmFjLmNu

†These authors have contributed equally to this work

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.