ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1482031
This article is part of the Research TopicEpigenetics and multi-omics studies of important traits in livestockView all 6 articles
MicroRNA analysis of porcine muscle tissue involved in phosphoinositol metabolism
Provisionally accepted
- 1Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming, Yunnan Province, China
- 2College of Biology and Agriculture, Zunyi Normal College, Zunyi, Guizhou Province, China
- 3Shanghai Laboratory Animal Research Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Introduction:MicroRNA(miRNAs), a crucial component of post-transcriptional regulation in eukaryotes, comprises a class of endogenous small non-coding single-stranded RNA molecules approximately 22 nucleotides in length. In order to elucidate the role of miRNAs in porcine muscle, this study used Solexa high-throughput sequencing to identify and compare differentially expressed miRNAs in Landrace pigs with higher lean meat percentage and in Diannan small-eared pigs with higher muscle fat deposition.Methods: We constructed small RNA libraries from high-quality RNA extracted from the latissimus dorsi muscles of two pig breeds and performed high-throughput sequencing to identify and compare differentially expressed miRNAs (DEmiRNAs). Then, the target genes corresponding to the differentially expressed miRNAs were subjected to functional annotation, Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genomes (KEGG) pathway enrichment analysis.Results:The research identified 241 known conserved miRNAs sequences and 20 novel miRNAs within the library of Diannan small ear pigs, while the Landrace pigs' library contained 251 known conserved miRNAs sequences and 22 novel miRNAs. Notably, 14 miRNAs exhibited significant differential expression between the two pig breeds, with expression levels of these miRNAs being lower in Diannan small ear pigs compared to Landrace pigs; the most pronounced difference was observed in miR-27a. Discussion:GO and KEGG analyses indicated that 26 DEmiRNAs target genes are implicated in inositol phosphate metabolism and the phosphatidylinositol signaling pathway. Among them, 20 target genes, including INPPL1, INPP5J,
Keywords: pig muscle tissue, microRNA, phosphoinositol metabolism, Diannan small ears pig, landrace pig INPP5A, INPP5B, INPP4A, INPP4B
Received: 17 Aug 2024; Accepted: 04 Jun 2025.
Copyright: © 2025 Xie, Wenjie, Hao, Yi, Zhu, Zhao and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Sumei Zhao, Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming, Yunnan Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.