ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1501997
This article is part of the Research TopicGlobal Perspectives on Swine Diseases: Detection, Diagnosis, and EradicationView all articles
Emergence and transmission of Carbapenem-Resistant Enterobacteriaceae blaNDM-5 gene in healthy pigs in Baise, Guangxi, China: A discovery
Provisionally accepted
Yuan yuan Dai1
Fei wen Wei2Zhen xue Li2Yuan chao Yuan2Juan li Zhao3
Yan-qiang Huang1Ming Hao1Ying Deng1Ji Yuan Teng1
Li Xue Yi1*- 1Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China
- 2Baise Center for Animal Disease Prevention and Control, baise, China
- 3Baise Agriculture and Rural Bureau, baise, China
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A total of 366 tonsillar tissue samples were collected from healthy free-range pigs owned by farmers across 12 districts in Baise City, Guangxi, China. This initiative successfully isolated six strains of Carbapenem-Resistant Enterobacteriaceae (CRE), including four strains of Escherichia coli, and one of Klebsiella aerogenes and Morganella morganii. Assessments utilizing Carbapenemase inhibitor enhancement test, in conjunction with the ResFinder resistance gene database, revealed that five strains carried the blaNDM-5 gene, while one strain possessed the blaNDM-1 gene. The overall positivity rate for CRE was determined to be 1.64%. Conjugation tests further demonstrated the ability of the blaNDM genes to transfer to recipient strains.Additionally, an analysis of the genetic environment surrounding the blaNDM genes showed similarities among the genes within the same geographic region, suggesting that these strains may have originated from a common ancestor and spread horizontally among different clones. This study provides valuable insights into the transmission of CRE among free-range farmers in Baise City, Guangxi, China.Six isolates of carbapenem-resistant Enterobacterales (CRE) from tonsil tissues of healthy pigs from 12 counties (cities and districts) within Baise City, Guangxi, China were confirmed to harbor carbapenem resistance gene blaNDM. Conjugation test revealed that blaNDM can transfer to receipt strain, and blaNDM resistance genetic environment showed that the similarity of the blaNDM genetic environment from the same geographic location, suggests that they may initially have come from the same ancestor and spread to different clones horizontally. This study provides valuable insight into CRE transmission across free-range farmers of Baise City, Guangxi, China.
Keywords: cre, Carbapenems, BlaNDM, resistance transmission, Swine
Received: 09 Oct 2024; Accepted: 15 Apr 2025.
Copyright: © 2025 Dai, Wei, Li, Yuan, Zhao, Huang, Hao, Deng, Teng and Yi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Li Xue Yi, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China
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