ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1536913
Investigation of Truncated Replication Protein Mutant of Canine Circovirus: Synergistic Interaction with Feline Panleukopenia Virus
Provisionally accepted
Suyao Li1
Jialiang Xin1
Yi Peng2
Haifeng Liu1
Ziyao Zhou1
Zhijun Zhong1Hualin Fu3Min Yang4Wancheng Li1
Ruihu Wu1
Guangneng Peng1*- 1Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, China
- 2College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan Province, China
- 3Department of Pharmacy, College of Veterinary Medicine, Sichuan Agriculture University, Chengdu, Sichuan Province, China
- 4College of Veterinary Medicine, Sichuan Agricultural University, Yaan Shi, Sichuan, China
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Background: Canine Circovirus (CanineCV) is a non-enveloped, single-stranded circular DNA virus in the Circoviridae family, known to cause respiratory and diarrheal diseases in dogs. It can also lead to immune suppression, which may worsen symptoms during co-infection. The virus's Replication (Rep) and Capsid (Cap) proteins play crucial roles in its life cycle. This study explores a novel truncated Rep' mutant of CanineCV and examines its impact on feline health when co-infected with Feline Panleukopenia Virus (FPV). Method: We constructed and validated clones and plasmids for CanineCV/SC49 and CanineCV/SC50. Virus particles were visualized using transmission electron microscopy (TEM), while quantitative polymerase chain reaction (qPCR) assessed viral load. Additionally, we examined the effects of Rep and Rep' proteins on cellular viability, their roles in FPV replication, and the host interferon type I (IFN-I) response. Results: CanineCV/SC50 enhanced FPV replication, with both Rep and Rep' proteins independently promoting this process. While Rep' exhibited lower cytotoxicity than Rep, it still negatively impacted cell viability. Additionally, both Rep and Rep' effectively suppressed the host's IFN-I response, highlighting their critical role in immune evasion.This study highlights the dual roles of canine circovirus in affecting host cell viability.It not only enhances the replication of co-infecting viruses but also suppresses the host's antiviral responses. These findings offer valuable insights into the pathogenic mechanisms of canine circovirus and emphasize the importance of understanding viral dynamics in co-infection contexts.
Keywords: canine circovirus, Replication protein, Truncated Replication Protein, Feline panleukopenia virus, synergistic effect
Received: 24 Jan 2025; Accepted: 12 May 2025.
Copyright: © 2025 Li, Xin, Peng, Liu, Zhou, Zhong, Fu, Yang, Li, Wu and Peng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Guangneng Peng, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan Province, China
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