ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. One Health
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1552097
This article is part of the Research TopicResearch Advances toward One Health in BrucellosisView all 10 articles
Preparation and application of specific chicken yolk antibodies in detecting Brucella
Provisionally accepted
- 1Xuzhou Medical University, Xuzhou, China
- 2Second People's Hospital of Huai'an, Huai'an, China
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Brucellosis, caused by Brucella, is a severe zoonotic disease. Conventional IgG antibody-based ELISA testing faces challenges such as false positives and crossreactivity. In this study, three specific chicken yolk antibodies (IgY) targeting Brucella were isolated from the eggs of immunized hens, and a method for detecting Brucella utilizing these antibodies was developed and subsequently compared to traditional IgG antibodies. These IgY antibodies were generated against a fusion protein, LPS, and whole-cell antigen, and their potency was evaluated through indirect ELISAs. Testing was conducted to assess cross-reactivity, limit of detection, and detection in simulated samples. The IgY antibodies demonstrated high potency and no cross-reactivity with common foodborne pathogens. Both LPS-IgY and Brucella-IgY showed excellent detection capabilities in identifying Brucella, particularly in food samples. These results underscore the potential of using LPS-IgY and Brucella-IgY antibodies as replacements for conventional IgG antibodies in Brucella detection, especially in the realm of food safety. The implications of this study are significant, as it presents a promising alternative approach for detecting Brucella in food products, thereby reducing the risk of transmission and ensuring public health and safety.
Keywords: ：Brucella, detection, chicken yolk antibody, ELISA, fusion protein
Received: 27 Dec 2024; Accepted: 23 Apr 2025.
Copyright: © 2025 Qi, Wang, Huang, Zhao, Pei, Chen, Yin and Zhan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dehui Yin, Xuzhou Medical University, Xuzhou, China
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