REVIEW article
Front. Vet. Sci.
Sec. Comparative and Clinical Medicine
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1562004
Canine organoids: state-of-the art, translation potential for human medicine and plea for standardization
Provisionally accepted
- 1Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
- 2Cancer Research Institute Ghent (CRIG)-Veterinary Oncology Network (VON), Ghent, Belgium
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Organoids have already shown great promise as research tools in human medicine. However, in veterinary medicine, such applications are limited and largely confined to canine organoids. In the Cross Health context, the potential of canine organoids lies in the translation to human diseases, such as cancer. This review provides a state-of-the art, highlights the current challenges, and at first compares the reported culture conditions of canine organoids derived from both non-neoplastic and neoplastic tissue (i.e. tumoroids), identifying substantial gaps and discrepancies in used culture methods. We make a plea for the standardization of canine organoid culture characteristics and increased rigor in parameter reporting, which will ultimately enhance the reproducibility and applicability of canine organoids in both veterinary and human medicine, especially in the oncology field.
Keywords: Organoid, Tumoroid, dog, Cross Health, Culture conditions, methodological rigor, comparative medicine
Received: 16 Jan 2025; Accepted: 14 Apr 2025.
Copyright: © 2025 Verduijn, De Rooster, Meyer and Steenbrugge. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jonas Steenbrugge, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
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