ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Comparative and Clinical Medicine
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1563532
Understanding the Biochemical Impact of Leukoreduction on Canine pRBC Storage: A Focus on Reactive Oxygen Species and Storage Lesions
Provisionally accepted
- 1Gyeongsang National University, Jinju, Republic of Korea
- 2Jeonbuk National University, Jeonju, North Jeolla, Republic of Korea
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Transfusion therapy is vital for both humans and animals, though it poses significant risks, including the development of storage lesions in packed red blood cells (pRBCs). This study examines hematological and biochemical changes during the storage of canine pRBCs, focusing on intraerythrocytic reactive oxygen species (ROS) and the impact of pre-storage leukoreduction. Eleven pRBC units were each divided into two aliquots, resulting in a total of 22 units, eleven leukoreduced (LR-pRBC) and eleven non-leukoreduced (nLR-pRBC), which were analyzed over 42 days. Results showed increased hemolysis, lactic acidosis, and potassium efflux (All, P < 0.01), with more severe lesions in nLR-pRBCs due to leukocyte presence. Notably, intraerythrocytic ROS levels increased in both groups (P < 0.05), driven by hemoglobin autoxidation (P < 0.05), though they decreased in later storage stages due to hemolysis and membrane vesiculation. The study highlights that pre-storage leukoreduction mitigates storage lesions, suggesting its implementation to enhance pRBC storage safety. Further research is necessary to understand the role of antioxidant systems in controlling intraerythrocytic ROS and preventing storage lesions.
Keywords: Leukoreduction, Reactive Oxygen Species, storage lesion, dog, transfusion
Received: 20 Jan 2025; Accepted: 13 Aug 2025.
Copyright: © 2025 Shin, Kim, Shin, Bae, Park, Jung, Cho and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: DoHyeon Yu, Gyeongsang National University, Jinju, Republic of Korea
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.