ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Behavior and Welfare
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1603458
Heat Stress on Sperm Quality in Dogs: effect of natural antioxidant supplementation
Provisionally accepted
Lucrezia Forte*
Matteo Burgio
Annalisa Rizzo
Aristide Maggiolino
Alice Carbonari*Pasquale De Palo
Giovanni Michele Lacalandra
Vincenzo Cicirelli- University of Bari Aldo Moro, Bari, Italy
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Abstract Introduction: Heat stress negatively affects canine reproductive health by inducing oxidative stress and impairing sperm quality. This study assessed the efficacy of a polyphenolic extract from Loblolly pine (Pinus taeda) lignin (PTHL), in mitigating heat stress-induced oxidative damage and preserving sperm quality in dogs. Methods: Forty clinically healthy male dogs were divided into two groups: a control (CON) group receiving a standard diet and a PTHL-supplemented group for 90 days. During summer, dogs were exposed to natural heat stress, and key parameters, including serum biochemical markers, oxidative stress indicators, antioxidant enzyme activity, and sperm quality were evaluated. Mixed-effects models analyzed group, time, and interaction effects. Results and Discussion: PTHL supplementation significantly reduced plasma TBARS and seminal d-ROMs levels (P < 0.01), indicating lower lipid peroxidation and confirming its protective effects. Antioxidant capacity improved in the PTHL group, with increased activities of antioxidant enzyme activity (SOD, CAT, GSPx) and elevated plasma Ferric Reducing Antioxidant Power (FRAP) levels (P < 0.01). Notably, the PTHL group demonstrated higher progressive motility (P < 0.01) and a greater percentage of rapid-movement sperm (P < 0.01) at 90 days, indicating improved sperm function under heat stress. Conclusion: These findings suggest that PTHL enhances antioxidant defenses, mitigating heat stress-induced reproductive impairment. This natural strategy may improve fertility in dogs facing environmental challenges. Future studies should explore different dosages and extended supplementation to optimize its benefits.
Keywords: thermal stress, Dogs, Antioxidants, Semen quality, Biochemical profile
Received: 31 Mar 2025; Accepted: 15 Jul 2025.
Copyright: © 2025 Forte, Burgio, Rizzo, Maggiolino, Carbonari, De Palo, Lacalandra and Cicirelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Lucrezia Forte, University of Bari Aldo Moro, Bari, Italy
Alice Carbonari, University of Bari Aldo Moro, Bari, Italy
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