BRIEF RESEARCH REPORT article

Front. Vet. Sci.

Sec. Veterinary Epidemiology and Economics

Volume 12 - 2025 | doi: 10.3389/fvets.2025.1605316

This article is part of the Research TopicInnovations in Veterinary Diagnostics for Enhancing Animal Health and WelfareView all 5 articles

Novel multiplex PCR approach for the detection of Erysipelothrix rhusiopathiae, Streptococcus suis, and Staphylococcus hyicus in swine

Provisionally accepted
A ARUN PRINCE MILTONA ARUN PRINCE MILTON1*Sabia KhanSabia Khan1Srinivas KandhanSrinivas Kandhan1Torik BasarTorik Basar1Zakir HussainZakir Hussain1G Bhuvana PriyaG Bhuvana Priya2M SaminathanM Saminathan3L H PuiiL H Puii1Sandeep GhatakSandeep Ghatak1Samir DasSamir Das1*
  • 1The ICAR Research Complex for North Eastern Hill Region (ICAR RC NEH), Umiam, India
  • 2College of Agriculture, Central Agricultural University (Imphal), Kydremkulai, India
  • 3Indian Veterinary Research Institute (IVRI), Bareilly, Uttar Pradesh, India

The final, formatted version of the article will be published soon.

Select one of your emails

You have multiple emails registered with Frontiers:

Notify me on publication

Please enter your email address:

If you already have an account, please login

You don't have a Frontiers account ? You can register here

Erysipelothrix rhusiopathiae, Streptococcus suis, and Staphylococcus hyicus are neglected, emerging and zoonotic pathogens in pigs that share common clinical signs and lesions, particularly septicaemia and skin lesions, highlighting the need for differential diagnosis.Currently, no single diagnostic assay is available to test samples for confirmation of the presence of all three pathogens. To address this, a novel multiplex PCR-based detection assay was optimized and evaluated for the simultaneous detection of E. rhusiopathiae, S. suis, and S. hyicus targeting the, 23S rRNA, recN, and sodA genes, respectively. The optimized multiplex PCR demonstrated 100% analytical specificity, with a detection limit of 10 4 copies/µL for E. rhusiopathiae and S. hyicus, and 10 6 copies/µL for S. suis. The assay was further validated by comparison with previously established uniplex PCR assays and tested on field-collected pig blood samples to assess real-world applicability. The developed assay is rapid, specific and cost-effective and can serve as a handy diagnostic tool for testing suspect samples for these neglected bacterial pathogens of pigs.

Keywords: multiplex PCR, Erysipelothrix rhusiopathiae, Streptococcus suis, Staphylococcus hyicus, pigs

Received: 03 Apr 2025; Accepted: 16 Jun 2025.

Copyright: © 2025 MILTON, Khan, Kandhan, Basar, Hussain, Priya, Saminathan, Puii, Ghatak and Das. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
A ARUN PRINCE MILTON, The ICAR Research Complex for North Eastern Hill Region (ICAR RC NEH), Umiam, India
Samir Das, The ICAR Research Complex for North Eastern Hill Region (ICAR RC NEH), Umiam, India

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Supplementary Material