ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Epidemiology and Economics
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1608822
This article is part of the Research TopicSequencing and Phylogenetic Analysis as a Tool in Molecular Epidemiology of Veterinary Infectious Diseases - Volume IIView all 14 articles
Exogenous Jaagsiekte Sheep Retrovirus (JSRV) Inner Mongolia Strain: Whole-Genome Characterization and Viral Particle Packaging
Provisionally accepted
- Inner Mongolia Agricultural University, Hohhot, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Introduction: Ovine pulmonary adenocarcinoma (OPA) is a contagious lung tumor caused by the exogenous Jaagsiekte sheep retrovirus (exJSRV). Analysing the genome of the pathogen is crucial for developing OPA prevention and control measures. Due to the absence of exogenous genomic JSRV-related information in Inner Mongolia, we aimed to establish a specific technique for exJSRV genomic amplification. Methods: Target virions were purified using U3 hn-PCR (hemi-nested PCR) combined with density gradient centrifugation. Specific reverse transcription primers were designed using the low-identity region of the internal and external genome, combined with long fragment PCR and 3'RACE technology, and the full-length genome of exogenous JSRV from Inner Mongolia was successfully obtained. Results: Exogenous molecular characteristics were found in the long terminal repeat（LTR）-U3 region, gag-variable region 1/2( VR1/VR2) and env-VR3, and was 98.8% identical to the Chinese JSRV-C1, which was significantly higher than that of foreign isolates (93.05%–95.84%) and enogenous Jaagsiekte sheep retrovirus (enJSRV) (88.73%–92.26%). Phylogenetic analysis showed that NMJS12 and exogenous JSRV-C1 were located in the same evolutionary clade. Accordingly, the whole genome eukaryotic expression plasmid was successfully constructed and viral particle packaging was achieved in 293T cells. Conclusion: Altogether, this study represents the first elucidation of the complete genome of exogenous JSRV in Inner Mongolia, China and provides a critical material foundation for antiviral target screening and research on OPA pathogenesis.
Keywords: Ovine pulmonary adenocarcinoma, exogenous Jaagsiekte sheep retrovirus, endogenous Jaagsiekte sheep retrovirus, Genomic sequencing, phylogenetic analysis, virus particles
Received: 06 May 2025; Accepted: 22 Jul 2025.
Copyright: © 2025 Zhang, Wang, Duan, Chen, Du, Bao, Ma, ZHANG and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shuying Liu, Inner Mongolia Agricultural University, Hohhot, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.