Morphological characterization, histopathological alteration, and cytokine response of different tissues of Columba livia naturally infected with Haemoproteus columbae

Heba M. Salem¹Amira M. Ibrahim^2,3Sara S. Barsoum²Mahmoud A. Mahmoud⁴Haleema H. Albohiri⁵Mina A. Almayouf⁶Layla A. Almutairi⁷Mohammed A. Alqahtani⁸Sultan Mohammed Areshi⁹Khaled A. El-Tarabily^10*Marwa M. Attia⁴

• ¹Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
• ²Faculty of Science, Fayoum University, Fayoum, Egypt
• ³Department of Poultry and Rabbits Diseases, Animal Health Research Institute (Egypt), zagzaig, Egypt
• ⁴Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Giza, Egypt
• ⁵Department of Biological Sciences, College of Science, Jeddah University, Jeddah, Saudi Arabia
• ⁶Department of Biology, College of Science and Arts at Unaizah, Qassim University, Buraidah, Saudi Arabia
• ⁷Department of Health Sciences, College of Health and rehabilitation sciences, Princess Nourah bint Abdulrahman University, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
• ⁸Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia
• ⁹Department of Biology, College of Science, Jazan University, Jazan, Jizan, Saudi Arabia
• ¹⁰Department of Biology, College of Science, United Arab Emirates University, AlAin, Abu Dhabi, United Arab Emirates

Introduction: Haemoproteus columbae is a common haemosporidian worldwide blood parasite affecting domestic pigeons (Columba livia). Therefore, this study aimed to detect the incidence of H. columbae infection in domestic pigeons with morpho-molecular identification.In the current study, blood samples were collected from 125 domestic pigeons between 2023 and 2024 and analyzed using both microscopic and molecular techniques. H. columbae positive birds underwent postmortem (PM) and histopathological examinations, as well as cytokine immunological reaction assessments.Results: It was found that around 8% (10/125) of pigeons were positive for H. columbae infection, and their morphological characteristics were reported. H. columbae induces observable macroscopic and microscopic alterations in the infected tissues, which increases the cytokine immunological reaction in the infected birds. The infected birds suffered from severe histopathological changes in most haemopoietic and parenchymatous organs. The transcript levels of inflammatory markers such as IL-6, IFN-γ, and IL-1β were significantly upregulated in H. columbae-infected birds. Additionally, the H. columbae samples' mRNA level of the apoptotic cas-3 indicated apoptotic activity.Discussion: Hematic parasites can pose a serious health threat to pigeons as they invade red blood cells and internal organs, leading to anemia, weakness, weight loss, and even death in severe cases. Epidemiological studies and surveys are essential for monitoring these hematologic parasites. Furthermore, additional research is recommended to evaluate the efficacy 3 of various herbal extracts in comparison to the most frequently used drugs for managing this issue in affected pigeons.