ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Oncology in Veterinary Medicine
This article is part of the Research TopicAdvancements and Challenges in Veterinary OncologyView all 12 articles
Preparation and functional validation of rabbit anti-canine CD3ε monoclonal antibody
Provisionally accepted
- 1Henan Agricultural University, Zhengzhou, China
- 2Henan Ruipai Pet hospital, zhengzhou, China
- 3Chongqing Medical University, Chongqing, China
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CD3, a surface antigen critical for T cell activation and signal transduction, serves as a key diagnostic marker for T cell-associated malignancies and a therapeutic target in immunotherapy. With canine models gaining prominence in translational immunology and oncology, reliable tools to study T cell-mediated immunity are essential. In this study, recombinant canine CD3ε protein was generated via eukaryotic expression and used to immunize rabbits, yielding a novel rabbit anti-canine CD3ε monoclonal antibody (mAb), designated HORCF-CD3.1. Functional characterization confirmed its specificity through flow cytometry and Western blot, demonstrating robust binding to CD3 molecules. Furthermore, the mAb effectively induced T cell stimulation in vitro when applied as an anti-CD3 activator. These findings validate HORCF-CD3.1 as a versatile tool for ELISA, Western blot, and flow cytometry applications. The successful development of this species-specific antibody provides a foundation for advancing diagnostic and therapeutic strategies targeting T cell-related diseases in both humans and dogs.
Keywords: canine, CD3, Monoclonal antibody, Immunotherapy, animal model
Received: 15 Apr 2025; Accepted: 24 Oct 2025.
Copyright: © 2025 李, Chen, Li, Shi, Qin, Meng and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shanshan Xie, xiess@henau.edu.cn
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