ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Comparative and Clinical Medicine
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1626574
Mouse Models for Enteral and Parenteral Nutrition after Surgery
Provisionally accepted
- 1Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, WuHan, China
- 2Xi'an Eighth's Hospital, Xi'an, China
- 3Qingdao Municipal Hospital, Qingdao, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background: This study aims to establish a mouse model with different nutritional support modalities (enteral nutrition and parenteral nutrition) after abdominal surgery, providing a stable and effective animal model for further study on the intestinal barrier damage caused by total parenteral nutrition.Methods: Twenty mice were randomly arranged into enteral nutrition (EN) group (n=10) or total parenteral nutrition (TPN) group (n =10). After the abdominal surgery via appendectomy, the two groups of mice received different modes of nutritional support (TPN or EN). A central venous catheter was placed in the right internal jugular or an enteral nutrition catheter was inserted into the duodenum. Parenteral or enteral nutrition was infused through these catheters. The survival rate, intestinal villus height, epithelial cell proliferation, changes in goblet cells and Paneth cells, as well as expression levels of the tight junction protein ZO-1 and occludin, were observed.No difference was found in survival rate(P < 0.01) between the two groups.However, significant differences were observed in the height of small intestinal villi, epithelial proliferation rate, number of goblet cells (PAS+), Paneth cell function (Lysozyme+), and expression of ZO-1 and occludin proteins. All of these markers were significantly decreased in the TPN group compared to the EN group (P ＜0.05).The mouse model is suitable and effective for investigating the pathogenesis of intestinal barrier function, as it provides different types of nutritional support after abdominal surgery.
Keywords: Total parenteral nutrition, Enteral Nutrition, mouse model, intestinal barrier, ZO-1
Received: 14 May 2025; Accepted: 25 Aug 2025.
Copyright: © 2025 Kong, Kong, Hu, Liao, Xiong and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Tao Fu, Qingdao Municipal Hospital, Qingdao, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.