The impact of age on the Na:K ratio: observations from a general canine population

Polina Zemko^1*Federico Bonsembiante¹Marco Canevelli²Tommaso Banzato¹

• ¹University of Padua, Padua, Italy
• ²Sapienza University of Rome, Roma, Italy

Background: The sodium-to-potassium (Na:K) ratio is commonly used as a screening criterion for hyponatremic and/or hyperkalemic hypoadrenocorticism (HA), a serious endocrine disorder in dogs characterized by non-specific clinical signs and variable laboratory findings. A Na:K ratio below 27 typically prompts further investigation through adrenal function tests. However, previous studies suggest that serum K levels may increase with age, even in otherwise healthy dogs. The objective of this study is to evaluate the influence of age on the Na:K ratio, in order to determine whether age-related changes could impact the reliability of this ratio as a screening tool in adult and senior dogs. Materials and Methods: We analyzed biochemical and hematological data from 208 dogs, aged 5–16 years, enrolled in a longitudinal research project of general canine population. The data included their medical history before the control visit and during the 12-month follow-up period. Results: The prevalence of dogs with a Na:K ratio ≤27 was found to be 2.4±2.7% in dogs under 10 years and 12.8±7.0% in those over 10 years. None of the dogs with Na:K ratio ≤27 had clinical suspicion of HA, either at the time of initial evaluation or during the 6-months follow-up period. Serum K levels showed a modest but statistically significant age-related increase of 0.22±0.05 mEq/L every 5 years, while Na levels remained stable. As a result, the Na:K ratio declined by 1.5±0.3 points every 5 years. Serum K was moderately correlated with the plateletcrit (PCT) (r=0.39, p-value<0.00001) and PCT was found to increase by 5.9%±1.6% every 5 years. It was estimated that each 10% increase in PCT corresponded to 0.142±0.027 mEq/L rise in serum K. Conclusions: The prevalence of dogs with a Na:K ratio ≤27 increases with age, reducing the specificity of this threshold for diagnosing HA in older dogs — particularly when Na levels are within the normal range. This decline is due largely, though not exclusively, to age-related increases in PCT, as platelets release K during clotting.