ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Comparative and Clinical Medicine
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1648911
This article is part of the Research TopicBiomarkers of Health and Disease in Veterinary Science - Volume IIView all 6 articles
Targeted next-generation sequencing of Candidate Regions Identified by GWAS Revealed SNPs Associated with IBD in GSDs
Provisionally accepted
Atiyeh Peiravan1
Mazdak Salavati2
Androniki Psifidi1
Mellora Sharman3Andrew Kent4
Penny Watson5
Karin Allenspach6
Dirk Werling1*- 1Royal Veterinary College (RVC), London, United Kingdom
- 2Scotland's Rural College, Edinburgh, United Kingdom
- 3VetCT, St John’s Innovation Park, Cambridge, United Kingdom
- 4Blaise Referral, Birmingham, United Kingdom
- 5Emmanuel College, Cambridge, United Kingdom
- 6University of Georgia, Athens, United States
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Canine Inflammatory bowel disease (IBD) is a chronic multifactorial disease, resulting from complex interactions between the intestinal immune system, microbiota and environmental factors in genetically predisposed dogs. Previously, we identified several single nucleotide polymorphisms (SNP) and regions on chromosomes (Chr) 7, 9, 11 and 13 associated with IBD in German shepherd dogs (GSD) using GWAS and FST association analyses. Here, building on our previous results, we performed a targeted next-generation sequencing (NGS) of a two Mb region on Chr 9 and 11 that included 14 of the newly identified candidate genes, to identify potential functional SNPs that could explain these association signals. Furthermore, correlations between genotype and treatment response were estimated. Results revealed several SNPs in the genes for canine EEF1A1, MDH2, IL3, IL4, IL13 and PDLIM. Based on the known function of their human orthologues, these results further our insight into their potential contribution to the pathogenesis of IBD in dogs. In addition, several pathways involved in innate and adaptive immunity and inflammatory responses (i.e.T helper cell differentiation, Th1 and Th2 activation pathway, communication between innate and adaptive immune cells and differential regulation of cytokine production in intestinal epithelial cells by IL-17A and IL-17F), were constructed involving the gene products in the candidate regions for IBD susceptibility.Interestingly, some of the identified SNPs were present in only one outcome group, suggesting that different genetic factors are involved in the pathogenesis of IBD in different treatment response groups. This also highlights potential genetic markers to predict the response in dogs treated for IBD.
Keywords: inflammatory bowel disease, Chronic enteropathy, NGS, GSD, canine
Received: 17 Jun 2025; Accepted: 21 Jul 2025.
Copyright: © 2025 Peiravan, Salavati, Psifidi, Sharman, Kent, Watson, Allenspach and Werling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dirk Werling, Royal Veterinary College (RVC), London, United Kingdom
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