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ORIGINAL RESEARCH article

Front. Vet. Sci.

Sec. Veterinary Infectious Diseases

Volume 12 - 2025 | doi: 10.3389/fvets.2025.1652362

First molecular detection of Mycoplasma agassizii in captive tortoises in Portugal

Provisionally accepted
Manuel  LouroManuel Louro1Rui  PatrícioRui Patrício1,2,3André  PereiraAndré Pereira1,2,3,4,5Andreia  ValençaAndreia Valença1,2,3*Margarida  AlvesMargarida Alves1,3,5*
  • 1Faculty of Veterinary Medicine, Lusófona University-Lisbon University Center, Lisbon, Portugal
  • 2Instituto Politecnico da Lusofonia Escola Superior de Saude Protecao e Bem-Estar Animal, Lisbon, Portugal
  • 3Research in Veterinary Medicine (I-MVET), Faculty of Veterinary Medicine, Lusófona University-Lisbon University Center, Lisbon, Portugal
  • 410Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA-REAL), Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Lisbon, Portugal
  • 5Animal and Veterinary Research Center (CECAV), Faculty of Veterinary Medicine, Lusófona University - Lisbon University Centre, Lisbon, Portugal

The final, formatted version of the article will be published soon.

Mycoplasma agassizii is a well-recognized etiologic agent of upper respiratory tract disease in tortoises. Although frequently reported in both captive and wild populations across Europe, its occurrence in Portugal had not been previously documented. This study aimed to investigate the presence of M. agassizii in apparently healthy captive tortoises in mainland Portugal and to evaluate potential host-and management-related factors associated with infection. Oral swabs were collected from 84 tortoises of 13 species across 3 geographic regions. DNA extraction success and sample integrity were confirmed by partial amplification of the tortoise mitochondrial 12S rRNA gene in 92.9% of cases (78/84), which were then screened for M. agassizii using a species-specific PCR targeting the 16S rRNA gene. The pathogen DNA was detected in 66.7% (52/78) of individuals. Phylogenetic analysis confirmed species identification, with all sequences forming a strongly supported monophyletic cluster together with M. agassizii reference sequences. A significant association was observed between tortoise genus and M. agassizii infection (p = 0.021), with Chelonoidis exhibiting a significantly lower infection frequency than Testudo (p = 0.029). No statistically significant associations were observed regarding geographic region, housing origin, or group size. These results reveal a high frequency of M. agassizii infection in apparently healthy captive tortoises in Portugal, emphasizing its potential for silent transmission in group or mixed-species settings. Our findings support the inclusion of this pathogen in the differential diagnosis of respiratory disease in tortoises, even when clinical signs are absent and underscore the need for routine molecular surveillance and strengthened biosecurity practices to mitigate transmission risks and foster chelonian conservation efforts.

Keywords: captive tortoises, chelonian health, Molecular Epidemiology, Mycoplasma agassizii, Polymerase Chain Reaction, Subclinical infection

Received: 23 Jun 2025; Accepted: 04 Aug 2025.

Copyright: © 2025 Louro, Patrício, Pereira, Valença and Alves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Andreia Valença, Faculty of Veterinary Medicine, Lusófona University-Lisbon University Center, Lisbon, Portugal
Margarida Alves, Faculty of Veterinary Medicine, Lusófona University-Lisbon University Center, Lisbon, Portugal

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