SARS-CoV-2 infection and exposure in cats and dogs in Romania

Andreea Paula Cozma¹Mihaela Anca Dascalu²Ioana Buzdugan²Oana Tanase²Geta Pavel³Christelle Enond⁴Noëlline Guillou⁵Juliane Peronnet⁴Lucia Carmen Trinca¹Smaranda Hristodorescu-Grigore⁶Anne-Geneviève Marcelin⁷Vincent Calvez⁷Stéphane Marot⁷Serban Morosan^2,8*

• ¹Department of Exact Sciences, Universitatea de Stiintele Vietii Ion Ionescu de la Brad din Iasi, Iași, Romania
• ²Department of Public Health, Universitatea de Stiintele Vietii Ion Ionescu de la Brad din Iasi, Iași, Romania
• ³Department of Preclinics, Universitatea de Stiintele Vietii Ion Ionescu de la Brad din Iasi, Iași, Romania
• ⁴UMS28, INSERM, Sorbonne Universite, Paris, France
• ⁵INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Sorbonne Universite, Paris, France
• ⁶Praxis Laboratory, Universitatea de Medicina si Farmacie Grigore T Popa lasi, Iași, Romania
• ⁷Institut Pierre Louis d'Épidémiologie et de Santé Publique (iPLESP), INSERM, Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Pitié-Salpêtrière, Service de Virologie, Sorbonne Universite, Paris, France
• ⁸UMS28, INSERM, Sorbonne Universités, Paris, France

SARS-CoV-2 has been described in more than 54 animal species, including wildlife, zoo animals and livestock. In the present study, conducted during 2021 and 2022 at the Faculty of Veterinary Medicine from Iasi, Romania, we studied the anthropogenic transmission of SARS-CoV-2 to pets by investigating active or prior infections of cats (n=41) and dogs (n=99) from the households of owners with confirmed COVID-19. Tests on an oropharyngeal swab from one cat revealed the presence of SARS-CoV-2 RNA 10 days after the onset of COVID-19 in its owner and another cat displayed SARS-CoV-2 seroconversion 15 days after the onset of COVID-19 in its owner but without the detection of SARS-CoV-2 viral RNA in its follow-up samples. Anti-N antibodies were detected in 7.2% (n=7) of dogs and 12.5% (n=5) of cats. All the seropositive cats were found to have SARS-CoV-2 neutralizing antibodies (NAbs) whereas only 42.9% (n=3) of dogs displayed specific NAbs. These results are consistent with global reports, confirming the cross-species transmission of SARS-CoV-2. However, there is no evidence to suggest that companion animals are involved in the spread of SARS-CoV-2 to humans rather than simply being accidental hosts. Nevertheless, we describe several cases of potential anthropogenic infections during the pre-Omicron SARS-CoV-2 variant era.