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ORIGINAL RESEARCH article

Front. Vet. Sci.

Sec. Veterinary Infectious Diseases

Volume 12 - 2025 | doi: 10.3389/fvets.2025.1674259

This article is part of the Research TopicAntiviral Innate Immune Mechanisms in Animal HostsView all 9 articles

Calycosin inhibits porcine reproductive and respiratory syndrome virus replication and activates RIG-Ⅰ/IRF3 signaling pathway

Provisionally accepted
Yafei  ChangYafei Chang1,2Zhaopeng  LiZhaopeng Li1Mengqi  WangMengqi Wang1Kanglei  PeiKanglei Pei1Xiaobo  ChangXiaobo Chang1*Jinyou  MaJinyou Ma1
  • 1College of Animal Science and Veterinary Medicine, Henan institute of Science and Technology, Xinxiang, China
  • 2Postdoctoral Innovation Practice Base, College of Animal Science and Veterinary Medicine, Henan Institue of Science and Technology, Xinxiang, China

The final, formatted version of the article will be published soon.

Porcine reproductive and respiratory syndrome virus (PRRSV), the causative pathogen of PRRS, remains one of the most important pathogens threatening the global pig industry. Due to the genetic diversity of PRRSV, the existing commercially vaccines cannot completely protect pigs from PRRSV infection. Flavonoid compounds play an important role in inhibiting viral replication. In this study, we found that calycosin, a natural active compound isolated from astragalus, could inhibit PRRSV replication regardless of whether calycosin was added pre-, co-, or post-PRRSV infection in vitro. Furthermore, coincubation of calycosin with PRRSV showed a better inhibitory effect compared to separate incubation, and calycosin mainly inhibited virus replication, assembly and release stages. Importantly, calycosin enhanced the antiviral immunity, as shown by the increased expression of IFN-β and ISG56. Subsequently, we discovered that calycosin promoted the expression of RIG-Ⅰ and MAVS, and induced the phosphorylation and nuclear translocation of IRF3 during PRRSV infection. Collectively, calycosin could markedly inhibit PRRSV replication and activate the RIG-Ⅰ/IRF3 signaling pathway. These data contribute to understanding the role of calycosin in PRRSV replication, and may provide valuable insights for the development of future antiviral strategies.

Keywords: PRRSV, Calycosin, Antiviral activity, IRF3, RIG-1

Received: 27 Jul 2025; Accepted: 01 Oct 2025.

Copyright: © 2025 Chang, Li, Wang, Pei, Chang and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Xiaobo Chang, changxiaobo88@163.com

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