ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Clinical, Anatomical, and Comparative Pathology
This article is part of the Research TopicInnovative Approaches in Veterinary Pathology: Diagnostics, Therapeutics, and Zoonotic ThreatsView all 15 articles
Porcine salivary carbonic anhydrase VI is involved on the pathogenesis of disease
Provisionally accepted
- 1University of Murcia, Murcia, Spain
- 2Veterinarmedizinische Universitat Wien, Vienna, Austria
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Evidence exists of the role of salivary carbonic anhydrase VI (CA VI) in the protection of the oral mucosa and in the gastrointestinal and respiratory tracts in different species. However, little is known of the behaviour of CA VI in porcine disease. We aimed to study the behaviour of salivary CA VI in porcine stress and disease for the first time and to study the concentrations of the CA VI in different homeostasis disturbances under field conditions. We used saliva samples from 30 healthy and 30 pigs with different disorders in a validation trial using protein separation and specific CA VI detection. Afterwards, the salivary CA VI concentrations of 255 clinically healthy pigs and 371 pigs with different signs of disease were quantified using a new in-house immunoassay in a field trial, in which salivary alpha amylase and C-reactive protein (CRP) levels were also evaluated. The overall specific CA VI signal appeared increased in disease condition in comparison to the healthy state while stress showed no statistical modification. The immunoassay developed for CA VI quantifications showed good analytical performance and revealed increased concentrations in all the diseases studied in comparison to healthy animals, with pronounced intensities in gastrointestinal and respiratory diseases. CA VI showed an overall moderate positive association with alpha amylase and a low but positive association with CRP. The present findings suggest that salivary CA VI is involved in the pathogenesis of disease in pigs with an increase in its concentrations, however the specific roles attributed of CA VI in disease conditions should be further studied.
Keywords: carbonic anhydrase VI, Disease, Health Status, pig, Saliva
Received: 29 Jul 2025; Accepted: 27 Nov 2025.
Copyright: © 2025 Matas Quintanilla, López-Balibrea, Miller and Gutiérrez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ana María Gutiérrez
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