ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Livestock Genomics
This article is part of the Research TopicGenomic Selection and Evolution in Domestic AnimalsView all 5 articles
Design, Validation, and Application of a 1K Liquid Chip for Genome-Wide Association Analysis in Alpine Merino Sheep
Provisionally accepted
- Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Because wool is an important animal fiber and mutton is rich in nutrients, fine wool sheep are one of the most economically important livestock. China has been selecting and breeding high-quality fine wool sheep breeds since the 1950s and is the largest processing and consuming country for fine wool sheep. In this study, blood samples were collected from Alpine Merino sheep (AMS), Chinese Merino sheep (CMS), Aohan fine wool sheep (AHS), and Qinghai fine wool sheep (QHS). Genomic DNA was extracted and subjected to high-depth genome resequencing. GenoBait technology was used for probe design and site optimization, followed by synthesis of a low-density liquid-phase chip. Finally, 409 AMS were randomly selected to verify the chip. The results showed that a total of 1012 single-nucleotide polymorphism sites (SNPs) were screened and retained for the synthesis of a low-density liquid chip for genome-wide selection and population genetics structure analysis. This chip can provide a useful tool for genome analysis of fine wool sheep and lay a solid foundation for subsequent breeding work.
Keywords: Fine wool sheep, targeted sequencing, genotyping, Liquid chip, Genome-WideAssociation Analysis
Received: 22 Aug 2025; Accepted: 10 Nov 2025.
Copyright: © 2025 TINGTING, Yuan, Jianbin and Bohui. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: GUO TINGTING, guotingting@caas.cn
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.