ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
The subunit vaccine of Lumpy Skin Disease Virus (LSDV) elicits significant humoral and cell-mediated immune responses in mice
Provisionally accepted
- 1Shihezi University, Shihezi, China
- 2Hunan University of Medicine, Huaihua, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Lumpy skin disease (LSD) is an emerging systemic and infectious disease affecting cattle. Currently, there is no specific treatment for this disease, and vaccination to boost immunity remains the most direct and effective approach for preventing LSD. In this study, we selected ORF073, ORF075, ORF090, and ORF110 proteins from the Lumpy skin disease virus (LSDV), which exhibit dominant antigenic properties, to construct, express, and identify recombinant prokaryotic expression vectors. The purified proteins were used to immunize mice. The immune efficacy of the subunit vaccines was preliminarily evaluated by monitoring antibody secretion and the expression of immune-related genes. The results showed that mice immunized with ORF075 and ORF090 subunit vaccines produced higher levels of antibody responses and induced a Th1/Th2-biased immune response. Splenocytes from immunized mice, when stimulated with vaccine antigens in vitro, exhibited the induction of higher levels of T-cell immune responses. These findings demonstrate that the LSDV ORF075 and ORF090 subunit vaccine developed in this study successfully induced robust humoral and cellular immune responses in mice, thereby providing a data foundation for subsequent experiments in the natural bovine host.
Keywords: LSDV, subunit vaccine, evaluation of immunogenicity, Mouse, Prokaryotic expression
Received: 24 Oct 2025; Accepted: 24 Nov 2025.
Copyright: © 2025 Gu, Deng, Wu, Li, Liu, Wang, Ma and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhongchen Ma
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.