Impact Factor 5.122 | CiteScore 4.04
More on impact ›

Original Research ARTICLE

Front. Bioeng. Biotechnol., 15 November 2018 | https://doi.org/10.3389/fbioe.2018.00171

Diamond/Porous Titanium Nitride Electrodes With Superior Electrochemical Performance for Neural Interfacing

Suzan Meijs1, Matthew McDonald2, Søren Sørensen3, Kristian Rechendorff3, Ladislav Fekete4, Ladislav Klimša4, Václav Petrák4, Nico Rijkhoff1, Andrew Taylor4, Miloš Nesládek2 and Cristian P. Pennisi5*
  • 1SMI, Department of Health, Science and Technology, Aalborg University, Aalborg, Denmark
  • 2Institute for Materials Research, University of Hasselt, Diepenbeek, Belgium
  • 3Materials Division, Danish Technological Institute, Århus, Denmark
  • 4Department of Functional Materials, Institute of Physics of the Czech Academy of Sciences, Prague, Czechia
  • 5Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark

Robust devices for chronic neural stimulation demand electrode materials which exhibit high charge injection (Qinj) capacity and long-term stability. Boron-doped diamond (BDD) electrodes have shown promise for neural stimulation applications, but their practical applications remain limited due to the poor charge transfer capability of diamond. In this work, we present an attractive approach to produce BDD electrodes with exceptionally high surface area using porous titanium nitride (TiN) as interlayer template. The TiN deposition parameters were systematically varied to fabricate a range of porous electrodes, which were subsequently coated by a BDD thin-film. The electrodes were investigated by surface analysis methods and electrochemical techniques before and after BDD deposition. Cyclic voltammetry (CV) measurements showed a wide potential window in saline solution (between −1.3 and 1.2 V vs. Ag/AgCl). Electrodes with the highest thickness and porosity exhibited the lowest impedance magnitude and a charge storage capacity (CSC) of 253 mC/cm2, which largely exceeds the values previously reported for porous BDD electrodes. Electrodes with relatively thinner and less porous coatings displayed the highest pulsing capacitances (Cpulse), which would be more favorable for stimulation applications. Although BDD/TiN electrodes displayed a higher impedance magnitude and a lower Cpulse as compared to the bare TiN electrodes, the wider potential window likely allows for higher Qinj without reaching unsafe potentials. The remarkable reduction in the impedance and improvement in the charge transfer capacity, together with the known properties of BDD films, makes this type of coating as an ideal candidate for development of reliable devices for chronic neural interfacing.

Introduction

Nanocrystalline diamond films synthesized by means of chemical vapor deposition (CVD) represent a unique class of materials with outstanding physical and chemical properties, including superior hardness and the ability to resist extreme corrosive environments (Williams, 2011). Besides these features, electrically conductive boron-doped diamond (BDD) exhibits a wide potential window and low background currents, which make it a fascinating material for electrochemical applications (Rao and Fujishima, 2000). During the last few decades, BDD has been employed for the fabrication of electrodes for a wide range of applications, including electroanalysis (Compton et al., 2003; Suzuki et al., 2007; Schwarzová-Pecková et al., 2017), electrosynthesis (Kraft, 2007; Ivandini and Einaga, 2017; Ashcheulov et al., 2018), and biosensing (Vermeeren et al., 2009; Zhou and Zhi, 2009; Qureshi et al., 2010; Svítková et al., 2016). More recently, BDD attracted attention as electrode material for neurochemical sensing, neural recording, and neural stimulation applications, both in vitro and in vivo (Hébert et al., 2014b; Garrett et al., 2016). Studies have shown that BDD microelectrodes are suitable for the measurement of bioelectric potentials from cultured mammalian neural cells (Ariano et al., 2005; McDonald et al., 2017) and from neural tissue in acute settings (Ho-Yin et al., 2009). BDD holds also great promise for the fabrication of implantable electrodes for chronic application, as the material exhibits extraordinary physical stability, biocompatibility, and resistance to protein biofouling in vivo (Alcaide et al., 2016b; Meijs et al., 2016a). However, in contrast to conventional electrode materials, planar BDD films display relatively lower double layer capacitance, and high impedance (Swain, 1994; Alehashem et al., 1995). This is a drawback for neural stimulation applications, as the amount of charge that can be effectively injected through electrodes with relatively small contact sites is quite limited.

Several approaches have been proposed to increase the effective electrochemical area of BDD films as means to boost the amount of charge that could be transferred through the interface. In classical top-down strategies, diamond films are typically etched under a reactive plasma atmosphere to increase their porosity (Yu et al., 2014). In this direction, Kiran et al. have demonstrated successful in vitro recording and stimulation of neural preparations using microelectrode arrays (MEAs) comprising “nanograss” BDD contact sites (Kiran et al., 2013). Although this approach has shown to achieve a moderate increase in the electrode capacitance, the fabrication method remains complex and time-consuming, compromising its industrial viability. Alternatively, in bottom-up strategies, a highly porous substrate is used as a template onto which thin diamond films are deposited. Some examples within the various types of porous templates include vertically aligned carbon nanotubes (Hébert et al., 2014a; Zanin et al., 2014), TiO2 nanostructures (Siuzdak et al., 2015), and SiO2 fibers (Petrák et al., 2017; Vlčková Živcová et al., 2018). Accordingly, BDD electrodes using 3 μm-long vertically aligned carbon nanotubes as an interlayer template have displayed a significant increase in charge storage capacity (CSC) and reduction in the impedance. This improvement in the electrochemical properties allowed successful stimulation and recording of electrical activity in excised mouse hindbrain preparations (Piret et al., 2015). However, integration of carbon nanotubes in implantable neural probes still faces some concerns, due to the risks of long-term cytotoxic effects and the mechanical damage that might occur during implantation (Musa et al., 2012; Liu et al., 2013).

Titanium nitride (TiN) is an attractive material, which can be applied for the fabrication of porous templates with high electrochemical surface area (ESA) by simple physical vapor deposition techniques. Porous TiN coatings have long been employed for pacemaker electrodes and have also been used for fabrication of neural stimulation and recording electrodes (Norlin et al., 2005; Specht et al., 2006; Meijs et al., 2015a). The porosity of TiN films can be easily controlled by adjusting the deposition parameters, such as gas composition, flow rate, and deposition time (Norlin et al., 2005; Cunha et al., 2009). The pores extend deep into the coating, resulting in a high ESA and a high CSC (Cunha et al., 2009). In a preliminary study, we have confirmed the feasibility of fabricating electrodes based on a thin-film BDD deposited on TiN and shown that these electrodes exhibited a relatively high CSC due to the wide potential window typical for BDD (Meijs et al., 2015b).

In this work, the aim is to identify deposition conditions that would allow fabricating BDD electrodes suitable for neural stimulation applications. A range of porous TiN electrodes was fabricated and subsequently deposited with a BDD thin-film. The morphology, quality, and surface properties of the resulting BDD/TiN films were characterized. In addition, we assessed the influence of the underlying TiN film parameters on the electrochemical performance of the electrodes by means of cyclic voltammetry (CV), voltage transient (VT) measurements, and electrochemical impedance spectroscopy (EIS).

Materials and Methods

Electrode Fabrication

The test samples were fabricated using a monopolar Ti6Al4V electrode pin, which belongs to a system intended for genital nerve stimulation (Martens et al., 2011). Seven types of TiN coatings were evaluated, which were deposited on the electrodes' contact sites by reactive DC magnetron sputtering. Deposition was carried out using an industrial coating unit (CC800, CemeCon AG, Germany) from two Ti targets (88 × 200 mm) with 99.5% purity in a mixed Ar/N2 atmosphere. In one set of samples (designated as samples I to V), the N2 flow was varied from 30 to 300 standard cubic centimeters per min (sccm), while the deposition time was kept constant at 27.5 × 103 s. In another set of samples (designated as samples III, VI, and VII), the flow rate of N2 was kept at 180 sccm while the deposition time was modified. In both cases, the Ar flow was kept constant at 180 sccm.

BDD thin films were synthesized on the TiN layers using an Astex AX6500 microwave plasma enhanced CVD system. The TiN-coated electrodes were first immersed in a 0.33 g/L solution of diamond nanoparticles (3.8 ± 0.7 nm) from Shinshu University to seed the surface for diamond growth. Hydrogen gas with an addition of 1% CH4 was added to the chamber at a total flow rate of 500 sccm. Tri-methyl boron was added to the gas as the dopant source, at boron to carbon concentrations of 10,000 ppm. The substrate temperature was maintained at ~750°C by using a pressure of 25 Torr (3.33 kPa) and a microwave power of 2,500 W.

Surface Characterization

The TiN thin-films were investigated using scanning electron microscopy (SEM) (Nova 600, FEI, The Netherlands). Detailed images of all electrodes were recorded at 80,000× magnification. For the assessment of film thickness, flat substrates (10 × 10 mm) obtained from silicon wafers were coated during deposition of each batch of electrodes. The silicon substrates were placed in a manner that ensured an even coating thickness. The coated substrates were subsequently broken and analyzed by cross sectional SEM. The thickness was measured via analysis of the SEM micrographs using Image J (NIH, Bethesda, MD). Each sample was measured at several locations along the cleavage to assess for thickness variations. Only small variations were observed and a unique thickness could unambiguously be assigned to each sample. The surface morphology of the BDD/TiN films was analyzed by a FERA3 GM SEM (Tescan, Czech Republic) with Schottky field emission cathode (FEG-SEM). Images were taken in the high-resolution mode at the accelerating voltage of 5 kV to minimize the interaction volume.

Raman spectroscopy of BDD/TiN films was carried out at room temperature using an InVia Raman Microscope (Renishaw ApS, Denmark) with the following conditions: wavelength = 325 nm, ×40 Olympus objective, 65 μm slits, spot focus, grating = 2,400 L/mm. A high pressure, high temperature Ib single crystal diamond was used as a reference for the sp3 Raman peak position.

Topography and surface roughness over a large area (220 × 280 μm2) was investigated by an optical profilometer (NewView 7200, ZYGO, Middlefield, CT). In addition, surface roughness and topography over a small area (5 × 5 μm2) were investigated by atomic force microscopy (AFM) using a Dimension Icon ambient AFM (Bruker, Germany) in peak force tapping mode using Tap150AL-g tips (BudgetSensors, Innovative Solutions Bulgaria).

Electrochemical Measurements

All electrochemical measurements were carried out in a three-electrode set-up, using the either the TiN or the BDD/TiN electrodes as working electrodes (0.06 cm2), a platinum foil counter electrode (50 cm2), and a Ag|AgCl reference electrode (1.6 cm2). Measurements were performed in Ringer's solution at room temperature.

Cyclic voltammetry was performed by cycling the electrode potential between the water window limits. These limits were determined by increasing and decreasing the electrode potential until an exponentially increasing current was observed using a sweep rate of 0.05 V/s. Measurements were made at 0.05, 0.1, 0.5, and 1.0 V/s; 10 cycles were recorded at each sweep rate. The cathodic CSC of the electrodes was found by calculating the surface area under the zero current axis. The electrochemical surface area to geometrical surface area (ESA/GSA) ratio was calculated by dividing the CSC of the porous coatings by the CSC of the corresponding smooth coating at a sweep rate of 0.05 V/s.

Voltage transient measurements were made using a cathodic-first bipolar symmetric current pulse with an interphase, during which no current was applied. Each phase had a phase width of 200 μs and the duration of the inter-phase was 40 μs. For analysis of the VTs, the OCP was set to 0 V and the IR drop was subtracted. The IR-drop was calculated for each phase by subtracting the potential at 20 μs after pulse cessation from the last data point of the respective phase. The pulsing capacitance (Cpulse) was calculated for each pulse using the following equation:

Istim=Cpulse×dVdt

where Istim is the stimulation current and dV/dt is the slope of the last 90% of the cathodic phase of the VT. The Cpulse of the type I TiN electrodes was determined at a current at which safe potential limits were reached. The Cpulse of the other electrodes was determined at a stimulation current of 20 mA.

Cyclic voltammetry and VT measurements were performed with VersaSTAT 3 potentio-galvanostat (Princeton Applied Research, USA). The impedance spectrum was measured from 0.1 Hz to 100 kHz, five points/decade using a sinusoidal measurement current of 5.0 μA. Impedance spectroscopy was performed using Solartron, Model 1294 in conjunction with 1260 Impedance/gain-phase Analyzer (Solartron Analytical, UK). Linear regression analyses of the CSC and Cpulse-values were performed in Prism 7 (GraphPad Software Inc, La Jolla, CA).

Results

Effect of Deposition Conditions on the Surface Properties of TiN Films

The influence of deposition parameters on thickness and morphology of the TiN films was investigated by depositing films at different partial pressures of N2 while keeping the deposition time at 27.5 × 103 s. The partial pressure of N2 in the deposition chamber was modified by varying the N2 flow rate. At the lowest flow rate, the TiN films displayed a relatively smooth surface (Figure 1A). These samples, designated as type I, were used as substrates for the planar reference coatings throughout the study. The remaining films, deposited at N2 flow rates ranging from 120 sccm and above, consisted of rough surfaces displaying pyramidal-like features whose lateral dimensions decreased at higher N2 flow rates (Samples II–V, Figure 1A). The porous TiN films comprise of a highly dense columnar-type structure, with pyramidal features at the top of the columns. The typical cross-section profile of porous TiN films is shown in the Supplementary Figure 1. Thickness displayed a non-monotonic dependence on the N2 flow rate (Figure 1C). The maximum film thickness was obtained at 180 sccm (sample type III), where the partial pressures of N2 and Ar are equal. Subsequently, the effect of deposition time on thickness and morphology of films was assessed by depositing films at a shorter and a longer time interval in relation to sample III (samples VI and VII). The column size (Figure 1B) as well as film thickness (Figure 1C) correlated directly to the deposition time.

FIGURE 1
www.frontiersin.org

Figure 1. Scanning electron microscopy (SEM) analysis of the seven types of prepared TiN films (I–VII). (A) Effect of varying the N2 flow rate on film morphology. For this set of samples (I–V), the deposition time was kept at 27.5 × 103 s. Films type I were used as smooth reference coatings. (B) Effect of varying the deposition time on film morphology, while keeping the N2 flow rate at 180 sccm. (C) Thickness of the films displayed in (A,B). Scale bar in SEM images represents 1 μm.

Assessment of the Surface Properties of BDD/TiN Films

Diamond thin-films were synthesized on all types of TiN coating (I–VII). SEM images (Figure 2A) along with AFM images (Figure 2B) show the morphology of BDD films grown on four representative substrates: smooth TiN (type I), and the three electrodes grown at a growth rate of 180 sccm (types III, VI, and VI). SEM images revealed that the BDD films had a uniform coverage on the TiN and displayed a nanocrystalline structure with a grain size of ~50 nm. Due to the electrode geometry, in situ BDD-film thickness measurements were not possible, however, deposition onto silicon substrates at identical conditions resulted in film thicknesses in the order of the grain size (i.e., ~50–70 nm).

FIGURE 2
www.frontiersin.org

Figure 2. Surface analysis of four representative electrodes (type I, III, VI, and VII). (A) Scanning electron microscopy (SEM) images displaying the uniform coverage of the nanocrystalline diamond layer. Scale bar represents 1 μm. (B) Atomic force microscopy (AFM) height images. Scale bar represents 1 μm. (C) AFM surface roughness measurements from the films displayed in (B) before (bare TiN) and after diamond deposition (BDD/TiN). (D) 325-nm Raman spectra showing the diamond-related peak between 1,320 and 1,328 cm−1. The spectra have been offset for clarity.

The large-scale topography of the diamond films, as measured using an optical profilometer over an area of 220 × 280 μm2, was governed by grooves on the underlying TiAlV substrate, which are ~20 μm wide and up to 400 nm high. The roughness on this scale was around 200–350 nm and it was not significantly influenced by the TiN or the BDD coating. However, the small-scale topography measured by AFM over an area of 5 × 5 μm2 was mostly governed by the topography of the TiN pyramidal structure. The roughness of the diamond layer, which measured on a flat surface was around 30 nm, had only a minimal influence on the topography of the BDD/TiN films. Figure 2C shows the RMS surface roughness of the selected sample types before and after BDD deposition. As the electrode surface is not flat the error in the estimation of the roughness is ~20% when measured on different areas on the pin.

Raman spectroscopy confirmed the synthesis of diamond films in all TiN substrates. Figure 2D displays the Raman spectra of BDD films grown on the selected sample types. In all spectra, a shifted diamond peak is observed at 1,320–1,328 cm−1 as well as broad features related to sp2 at 1,360 and 1,585 cm−1, i.e., the D and G bands.

Electrochemical Characterization

The water window potentials were obtained by CV and their values were typically −0.6 to 0.9 V for TiN and −1.3 to 1.2 V for BDD/TiN electrodes (vs. Ag|AgCl). Table 1 summarizes the cathodic CSC-values which were obtained at a sweep rate of 0.05 V/s. The CSC obtained at a slow sweep rate gives an insight into the entire ESA of the porous electrodes. Due to the wide potential window brought by the BDD coating, the CSC of the BDD/TiN electrodes was consistently higher than the CSC of bare TiN electrodes. The CSC-values pre- and post-BDD deposition followed a linear relationship with a slope of 2.2, indicating that the CSC-values of the electrodes doubled upon BDD deposition (Supplementary Figure 2). It was also noticed that the CSC-values were drastically increased in all porous samples as compared to the smooth reference electrodes, for which the CSC-values were 0.36 and 7.74 mC/cm2, respectively. Table 1 includes the ESA/GSA ratio, which reflects the relative increase in the ESA for each of the porous samples, before and after BDD deposition. Figure 3A displays representative CV curves from electrodes type III, VI, and VI, before and after BDD deposition, where it is possible to observe the relative increase in the CSC when TiN films become deposited with BDD. As shown in Figure 3B, the CSC of these electrodes appears to increase linearly with an increase in the TiN coating thickness. The CSC-values fit a linear regression with a slope of 6.7 mC/cm2.μm for the bare TiN films (r2 = 0.98) and 14.2 mC/cm2.μm for the BDD/TiN films (r2 = 0.99), but the slopes are not significantly different (P > 0.05).

TABLE 1
www.frontiersin.org

Table 1. Cathodic CSC of the bare TiN and BDD/TiN electrodes obtained at 0.05 V/s.

FIGURE 3
www.frontiersin.org

Figure 3. Cyclic voltammetry measurements of electrodes type III, VI, and VII before (bare TiN) and after BDD deposition (BDD/TiN). (A) Cyclic voltammograms showing that the safe potential limits are increased in BDD/TiN electrodes as compared to the bare counterparts. The potential limits are the same within the two electrode groups. (B) Cathodic charge storage capacity (CSC) of the electrodes as a function of the underlying TiN film thickness, showing that CSC-values were consistently increased after BDD deposition. Data from samples type III, VI, and VII are shown in red, green, and blue, respectively. Dotted lines represent the linear regression of the CSC-values.

Table 2 summarizes the Cpulse for all electrode types, which was derived from the VT measurements. Except for electrode type I, the Cpulse of the BDD/TiN electrodes was consistently lower than the Cpulse of the corresponding TiN electrodes. Figure 4A shows representative VT measurements on electrodes type III, VI, and VII, before and after BDD coating. As compared to the bare TiN electrodes, the lower Cpulse of BDD/TiN electrodes leads to larger electrode potentials. However, as the safe potential window for BDD is larger than for TiN, the amount of charge that can safely be injected will be higher for BDD/TiN than for TiN. Cpulse displays a negative trend for both TiN and BDD/TiN electrodes as a function of TiN coating thickness (Figure 4B). The Cpulse-values fit similar linear regressions, with a slope of −0.051 mF/cm2.μm for the bare TiN films (r2 = 0.82) and −0.052 mF/cm2.μm for the BDD/TiN films (r2 = 0.77).

TABLE 2
www.frontiersin.org

Table 2. Pulsing capacitance of the bare TiN and BDD/TiN electrodes.

FIGURE 4
www.frontiersin.org

Figure 4. Voltage transient measurements of electrodes type III, VI, and VII before (bare TiN) and after BDD deposition (BDD/TiN). (A) The voltage transients of the BDD/TiN electrodes were larger than those of the bare TiN electrodes, evidencing a decrease in the pulsing capacitance (Cpulse). (B) Cpulse of the electrodes as a function of the underlying TiN film thickness, showing that Cpulse-values were decreased after BDD deposition. Data from samples type III, VI, and VII are shown in red, green, and blue, respectively. Dotted lines represent the linear regression of the Cpulse-values.

As anticipated, increased film porosity significantly reduced the impedance of the electrodes (Figure 5). The impedance magnitudes of the TiN and BDD/TiN porous electrodes were only different at frequencies below 10 Hz. The greatest difference in impedance magnitude between TiN and BDD/TiN was at 100 mHz, where the BDD electrodes consistently had a higher impedance than the TiN electrodes. The impedance magnitude of both BDD and TiN electrodes decreased with increasing thickness. The lowest impedance magnitude for TiN with and without BDD coating were obtained using the electrode with the thickest TiN coating (type VII).

FIGURE 5
www.frontiersin.org

Figure 5. Impedance magnitude spectra of electrodes type I, III, VI, and VII before (bare TiN) and after BDD deposition (BDD/TiN). Data from electrodes type I, III, VI, and VII are shown in gray, red, green, and blue, respectively.

Discussion

A range of porous TiN films, to be used as templates, was deposited onto test electrodes by means of a physical vapor deposition. The smooth TiN reference films were fabricated using a low partial pressure of N2 to ensure a stoichiometric Ti/N ratio below 0.6, which has been shown to be unfavorable for columnar growth (Igasaki et al., 1978; Cunha et al., 2009). Formation of the columnar, highly porous structures was favored using N2 flow rates of 120 sccm and above. For the set of samples II–V, higher N2 flow rates resulted in decreased film columnar width. This effect has been associated to a reduced mobility of the deposited atoms as a consequence of a weakening in the argon bombardment (Arshi et al., 2012). Furthermore, increasing the N2 flow poisons the Ti targets to the extent where the entire target surface is covered in TiN. This effect decreases the deposition rate as the sputter yield is lower for TiN than for Ti (Berg and Nyberg, 2005), which also results in thinner films with smaller columns. The deposition rate appeared to be maximal at a N2 flow rate of 180 sccm, where the N2 flow rate equals that of Ar and it is assumed that deposition occurs at a stoichiometric Ti/N ratio. TiN films with stoichiometric Ti/N composition are usually preferred due to their optimal mechanical and electrical properties (Kang and Kim, 1999; Martinez et al., 2014). Samples III, VI, and VII were therefore deposited keeping the N2 flow constant and varying the deposition time to obtain porous films with similar crystalline composition but different thickness. A longer deposition time increased the columnar width, which is a result of the competitive growth where some columns grow at the expense of others. Such a growth is typically observed for coatings deposited at a relatively low temperature compared to the melting temperature of the coating material (Ohring, 2002). For the TiN films, increasing the N2 flow (in samples I–III) led to higher thickness and higher porosity, which was reflected as an increased ESA/GSA ratio. Further increase in N2 (sample IV) still gave an increase in area due to increased porosity, although the thickness was smaller. Going to higher N2 the growth rate was slower, so that the lower thickness dominated over the increased porosity and an overall decrease in the ESA/GSA was obtained. For the samples grown at constant gas flow rate (III, VI, and VII), film thickness, and ESA/GSA ratio were directly correlated.

Surface analysis of the BDD/TiN samples by SEM and AFM revealed homogeneous and high quality BDD films. The overall structure and topography of the films appeared similar to the bare TiN samples, suggesting that CVD deposition did not significantly affect the morphology of the TiN template. The uniform coverage of diamond crystallites indicates a highly cohesive diamond film. This is in agreement with previous studies, which have shown that TiN possesses several favorable properties for nucleation and growth of good quality CVD diamond films, including low diffusivity of carbon, compatible interatomic potential, and small lattice mismatch (Weiser et al., 1992; Kumar et al., 1997; Polini et al., 2006). In addition, since TiN exhibits a moderate interface reactivity, its surface is stable under high-temperature diamond–CVD deposition (Contreras et al., 2000). Moreover, given the similar thermal expansion coefficient of both materials, the interlayer stresses are minimal, which ensures the synthesis of highly adherent diamond layers (Kumar et al., 1997). Although we did not encounter any evidence of cracks or film delamination, future studies should further investigate the nature the BDD/TiN interlayer by appropriate techniques, as for instance transmission electronic microscopy (TEM). Concerning the Raman analysis, the shifts in diamond's Raman peak can be related to a variation of stress in layers, nonetheless its shift to lower wavenumbers is associated with increasing B incorporation in the lattice (Prawer and Nemanich, 2004). It is worth noting that the spectra in Figure 2D are representative only, i.e., the ratio of sp3/sp2 changes with measurement position. This apparent variation in sp3/sp2 is related to the fact that the BDD coating is very thin and therefore the grain boundary content is high.

The superior ESA of the TiN coatings used as porous templates is evident from the high ESA/GSA ratios and the drastic reduction in the impedance magnitudes. Thicker films had a consistently higher CSC, suggesting that pores extend into the entire depth of the coating, which is in agreement with previous studies (Norlin et al., 2005; Cunha et al., 2009). BDD deposition onto the TiN coatings significantly increased the CSC of the electrodes due to the wide potential window of diamond. The linear correlation between the CSC of the TiN and the BDD/TiN electrodes indicates that the diamond films did not block the pores. On the other hand, the Cpulse showed a negative correlation with film thickness, as the highest Cpulse-values were obtained with thinner coatings and smaller column width. The BDD films might therefore cause narrowing of the pores, with a consequent increase in the pore resistance. This effect decreases the pore depth that can be used under pulsing conditions (Cogan, 2008). Thus, increasing the coating thickness beyond a certain level would be less advantageous for electrical stimulation purposes. While Cpulse is decreased for BDD/TiN as compared to bare TiN electrodes, it is important to view this result in the light of the wide safe potential window of BDD (Garrett et al., 2011). The decrease in Cpulse after depositing BDD ranged from 67% to <1%, while the cathodic potential limit was more than doubled (−0.6 V for TiN vs. −1.3 for BDD/TiN). This means that the amount of charge that can be injected without reaching unsafe potentials is doubled by applying a BDD thin-film onto a porous TiN coating. It is important to view these results in the light of the intended application of the BDD/TiN electrodes, which is in vivo chronic neurostimulation. It has been shown that the stimulation performance of TiN electrodes deteriorates after implantation (Meijs et al., 2015a, 2016b,c). This is not the case for BDD electrodes, which display a remarkable resistance to protein biofouling (Trouillon and O'Hare, 2010; Alcaide et al., 2016a; Meijs et al., 2016a). Nevertheless, protein adsorption is influenced by surface topography, which warrants further investigation of the electrochemical performance of porous BDD/TiN electrodes in protein-rich environments.

The relatively low Cpulse and high impedance shown by the smooth BDD electrodes was evident and corresponds well to what has been shown in previous studies (Garrett et al., 2011; Meijs et al., 2013). Remarkably, the electrochemical performance of BDD displayed a significant improvement thanks to the large ESA gained by using the porous TiN templates. As previous studies have shown, other porous templates have been instrumental in enhancing the electrochemical performance of BDD (Bonnauron et al., 2008; Kiran et al., 2013; Hébert et al., 2014a). A notable example is the growth of BDD on vertically aligned nanotubes, which has shown to increase the CSC of BDD up to 10 mC/cm2 (Piret et al., 2015). The BDD/TiN electrodes in the current, however, displayed CSC-values up to 253 mC/cm2 for the type VII electrode. Furthermore, while the use of carbon nanotube based materials for human implants remains controversial due to evidence of cytotoxic effects (Smart et al., 2006; Liu et al., 2013), both TiN and BDD have demonstrated low risk of cytotoxicity and excellent biocompatibility in diverse applications. TiN is well known for improving the electrochemical and biocompatibility properties of various materials (Subramanian et al., 2011) and represents one of the coatings with a long history of clinical use for orthopedic implants (Gotman et al., 2014; van Hove et al., 2015). Data from implantation studies revealed that BDD electrodes are associated with no signs of chronic inflammation and a very thin fibrous capsule (Alcaide et al., 2016b). Taken together, these results indicate that this novel type of combined coating may be used to fabricate safe implants for clinical use. Furthermore, the method can be easily scaled-up, making the production process fast and cost-effective (Taylor et al., 2014, 2018). The production process is reproducible and clean, as both coatings are deposited under vacuum conditions. Overall, these factors make this novel type of coating particularly attractive for the development of commercially viable electrodes for neural interfacing.

To achieve an increased charge injection (Qinj), the production parameters are of critical importance, as the extra coating increases the pore resistance, which may deteriorate Qinj. This study suggests that specific deposition parameters are optimal for stimulation electrodes, as increased thickness and N2 flow only result to a certain extent in larger Cpulse and Qinj. The data suggests that BDD deposited onto thinner coatings with smaller columnar size results in better stimulation performance. A thicker coating, however, results in a high CSC and low impedance, which could be exploited for other purposes, such as electrical and electrochemical sensing. This highlights the versatility of the novel coating combination presented in this work.

Conclusion

In this work, we have fabricated a range of BDD/porous TiN electrodes with very high surface area, which exhibit a broad safe potential window and CSC-values which are superior to those reported in the literature for porous BDD electrodes. Electrodes with more porous and thick coatings were associated with higher CSC and lower impedance magnitudes, but the relatively limited Cpulse would make them more suited for sensing applications. On the other hand, relatively higher Cpulse were obtained with thinner films with small column size, which would result more favorable for stimulation applications. Although BDD/TiN electrodes displayed a higher impedance magnitude and a lower Cpulse as compared to the bare TiN electrodes, the wider potential window likely allows for higher Qinj without reaching unsafe potentials. These remarkable improvements, together with the known mechanical stability, resistance to biofouling and long-term in vivo stability of BDD films, makes this coating combination an ideal candidate for development of reliable devices for chronic neural interfacing. This novel type of coating is particularly attractive for the development of commercially viable electrodes due to the simplicity and the scalability of the approach.

Author Contributions

AT, MN, and CP conceived and designed the study. SM, MM, SS, and KR prepared the samples. MM, SS, KR, LF, LK, and AT obtained the data and performed the surface analysis of the films. SM and VP acquired and analyzed the electrochemistry data under supervision of NR. SM and CP drafted the manuscript and designed the figures. All authors contributed to the critical revision of the draft and approved the submitted version.

Funding

This work was supported by the EU through the project MERIDIAN (Micro and Nano Engineered Bi-Directional Carbon Interfaces for Advanced Peripheral Nervous System Prosthetics and Hybrid Bionics), contract number 280778-02, by the Danish National Advanced Technology Foundation, and by the Czech Science Foundation (contract 17-15319S). AFM and SEM equipment were partially funded by projects LM2016088 and LO1409, Ministry of Education, Youth and Sports, Czech Republic.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fbioe.2018.00171/full#supplementary-material

References

Alcaide, M., Papaioannou, S., Taylor, A., Fekete, L., Gurevich, L., Zachar, V., et al. (2016a). Resistance to protein adsorption and adhesion of fibroblasts on nanocrystalline diamond films: the role of topography and boron doping. J. Mater. Sci. Mater. Med. 27:90. doi: 10.1007/s10856-016-5696-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Alcaide, M., Taylor, A., Fjorback, M., Zachar, V., and Pennisi, C. P. (2016b). Boron-doped nanocrystalline diamond electrodes for neural interfaces: in vivo biocompatibility evaluation. Front. Neurosci. 10:87. doi: 10.3389/fnins.2016.00087

PubMed Abstract | CrossRef Full Text | Google Scholar

Alehashem, S., Chambers, F., Strojek, J. W., Swain, G. M., and Ramesham, R. (1995). Cyclic voltammetric studies of charge transfer reactions at highly boron-doped polycrystalline diamond thin-film electrodes. Anal. Chem. 67, 2812–2821. doi: 10.1021/ac00113a014

CrossRef Full Text | Google Scholar

Ariano, P., Baldelli, P., Carbone, E., Gilardino, A., Lo Giudice, A., Lovisolo, D., et al. (2005). Cellular adhesion and neuronal excitability on functionalised diamond surfaces. Diam. Relat. Mater. 14, 669–674. doi: 10.1016/j.diamond.2004.11.021

CrossRef Full Text | Google Scholar

Arshi, N., Lu, J., Joo, Y. K., Lee, C. G., Yoon, J. H., and Ahmed, F. (2012). Study on structural, morphological and electrical properties of sputtered titanium nitride films under different argon gas flow. Mater. Chem. Phys. 134, 839–844. doi: 10.1016/j.matchemphys.2012.03.078

CrossRef Full Text | Google Scholar

Ashcheulov, P., Taylor, A., Mortet, V., Poruba, A., Le Formal, F., Krýsová, H., et al. (2018). Nanocrystalline boron-doped diamond as a corrosion resistant anode for water oxidation via si photoelectrodes. ACS Appl. Mater. Interfaces 10, 29552–29564. doi: 10.1021/acsami.8b08714

PubMed Abstract | CrossRef Full Text | Google Scholar

Berg, S., and Nyberg, T. (2005). Fundamental understanding and modeling of reactive sputtering processes. Thin Solid Films 476, 215–230. doi: 10.1016/j.tsf.2004.10.051

CrossRef Full Text | Google Scholar

Bonnauron, M., Saada, S., Rousseau, L., Lissorgues, G., Mer, C., and Bergonzo, P. (2008). High aspect ratio diamond microelectrode array for neuronal activity measurements. Diam. Rel. Mat. 17, 1399–1404. doi: 10.1016/j.diamond.2007.12.065

CrossRef Full Text | Google Scholar

Cogan, S. F. (2008). Neural stimulation and recording electrodes. Annu. Rev. Biomed. Eng. 10, 275–309. doi: 10.1146/annurev.bioeng.10.061807.160518

PubMed Abstract | CrossRef Full Text | Google Scholar

Compton, R. G., Foord, J. S., and Marken, F. (2003). Electroanalysis at diamond-like and doped-diamond electrodes. Electroanalysis 15, 1349–1363. doi: 10.1002/elan.200302830

CrossRef Full Text | Google Scholar

Contreras, O., Hirata, G. A., and Avalos-Borja, M. (2000). Interface analysis of CVD diamond on TiN surfaces. Appl. Surf. Sci. 158, 236–245. doi: 10.1016/S0169-4332(00)00014-3

CrossRef Full Text | Google Scholar

Cunha, L. T., Pedrosa, P., Tavares, C. J., Alves, E., Vaz, F., and Fonseca, C. (2009). The role of composition, morphology and crystalline structure in the electrochemical behaviour of TiNx thin films for dry electrode sensor materials. Electrochim. Acta 55, 59–67. doi: 10.1016/j.electacta.2009.08.004

CrossRef Full Text | Google Scholar

Garrett, D. J., Ganesan, K., Stacey, A., Fox, K., Meffin, H., and Prawer, S. (2011). Ultra-nanocrystalline diamond electrodes: optimization towards neural stimulation applications. J. Neural Eng. 9:016002. doi: 10.1088/1741-2560/9/1/016002

PubMed Abstract | CrossRef Full Text | Google Scholar

Garrett, D. J., Tong, W., Simpson, D. A., and Meffin, H. (2016). Diamond for neural interfacing: a review. Carbon 102, 437–454. doi: 10.1016/j.carbon.2016.02.059

CrossRef Full Text | Google Scholar

Gotman, I., Gutmanas, E. Y., and Gotman, I. (2014). Titanium nitride-based coatings on implantable medical devices. Adv. Biomater. Devices Med. 1, 53–73.

Google Scholar

Hébert, C., Mazellier, J. P., Scorsone, E., Mermoux, M., and Bergonzo, P. (2014a). Boosting the electrochemical properties of diamond electrodes using carbon nanotube scaffolds. Carbon 71, 27–33. doi: 10.1016/j.carbon.2013.12.083

CrossRef Full Text | Google Scholar

Hébert, C., Scorsone, E., Bendali, A., Kiran, R., Cottance, M., Girard, H. A., et al. (2014b). Boron doped diamond biotechnology: from sensors to neurointerfaces. Faraday Discuss. 172, 47–59. doi: 10.1039/C4FD00040D

PubMed Abstract | CrossRef Full Text | Google Scholar

Ho-Yin, C., Aslam, D. M., Wiler, J. A., and Casey, B. (2009). A novel diamond microprobe for neuro-chemical and -electrical recording in neural prosthesis. J. Microelectromech. Syst. 18, 511–521. doi: 10.1109/JMEMS.2009.2015493

CrossRef Full Text | Google Scholar

Igasaki, Y., Mitsuhashi, H., Azuma, K., and Muto, T. (1978). Structure and electrical properties of titanium nitride films. Jpn. J. Appl. Phys. 17, 85–96. doi: 10.1143/JJAP.17.85

CrossRef Full Text | Google Scholar

Ivandini, T. A., and Einaga, Y. (2017). Polycrystalline boron-doped diamond electrodes for electrocatalytic and electrosynthetic applications. Chem. Commun. 53, 1338–1347. doi: 10.1039/C6CC08681K

PubMed Abstract | CrossRef Full Text | Google Scholar

Kang, J. H., and Kim, K. J. (1999). Structural, optical, and electronic properties of cubic TiNx compounds. J. Appl. Phys. 86:346. doi: 10.1063/1.370736

CrossRef Full Text | Google Scholar

Kiran, R., Cottance, M., Joucla, S., Rousseau, L., Bongrain, A., Yvert, B., et al. (2013). “Nanograss boron doped diamond microelectrode arrays for recording and stimulating neuronal tissues,” in Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (Barcelona: IEEE), 748–751.

Google Scholar

Kraft, A. (2007). Doped diamond: a compact review on a new, versatile electrode material. Int. J. Electrochem. Sci. 2, 355–385.

Google Scholar

Kumar, A., You, Q., Kapat, J., Mangiaracina, A., Catletge, A., and Vohra, Y. (1997). Evaluation of buffer layers for hot filament chemical vapor deposition diamond films on silicon substrates. Thin Solid Films 308–309, 209–214. doi: 10.1016/S0040-6090(97)00669-X

CrossRef Full Text | Google Scholar

Liu, Y., Zhao, Y., Sun, B., and Chen, C. (2013). Understanding the toxicity of carbon nanotubes. Acc. Chem. Res. 46, 702–713. doi: 10.1021/ar300028m

PubMed Abstract | CrossRef Full Text | Google Scholar

Martens, F. M., Heesakkers, J. P., and Rijkhoff, N. J. (2011). Minimal invasive electrode implantation for conditional stimulation of the dorsal genital nerve in neurogenic detrusor overactivity. Spinal Cord 49, 566–572. doi: 10.1038/sc.2010.134

PubMed Abstract | CrossRef Full Text | Google Scholar

Martinez, G., Shutthanandan, V., Thevuthasan, S., Chessa, J. F., and Ramana, C. V. (2014). Effect of thickness on the structure, composition and properties of titanium nitride nano-coatings. Ceram. Int. 40, 5757–5764. doi: 10.1016/j.ceramint.2013.11.014

CrossRef Full Text | Google Scholar

McDonald, M., Monaco, A., Vahidpour, F., Haenen, K., Giugliano, M., and Nesladek, M. (2017). Diamond microelectrode arrays for in vitro neuronal recordings. MRS Commun. 7, 683–690. doi: 10.1557/mrc.2017.62

CrossRef Full Text | Google Scholar

Meijs, S., Alcaide, M., Sørensen, C., McDonald, M., Sørensen, S., Rechendorff, K., et al. (2016a). Biofouling resistance of boron-doped diamond neural stimulation electrodes is superior to titanium nitride electrodes in vivo. J. Neural Eng. 13:056011. doi: 10.1088/1741-2560/13/5/056011

PubMed Abstract | CrossRef Full Text | Google Scholar

Meijs, S., Fjorback, M., Jensen, C., Sørensen, S., Rechendorff, K., and Rijkhoff, N. J. (2015a). Electrochemical properties of titanium nitride nerve stimulation electrodes: an in vitro and in vivo study. Front. Neurosci. 9:268. doi: 10.3389/fnins.2015.00268

PubMed Abstract | CrossRef Full Text | Google Scholar

Meijs, S., Fjorback, M., Jensen, C., Sørensen, S., Rechendorff, K., and Rijkhoff, N. J. M. (2016b). Influence of fibrous encapsulation on electro-chemical properties of TiN electrodes. Med. Eng. Phys. 38, 468–476. doi: 10.1016/j.medengphy.2016.02.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Meijs, S., McDonald, M., Sørensen, S., Rechendorff, K., Petrák, V., Nesládek, M., et al. (2015b). “Increased charge storage capacity of titanium nitride electrodes by deposition of boron-doped nanocrystalline diamond films,” in NEUROTECHNIX 2015 - Proceedings of the 3rd International Congress on Neurotechnology, Electronics and Informatics (Lisbon).

Google Scholar

Meijs, S., Sørensen, C., Sørensen, S., Rechendorff, K., Fjorback, M., and Rijkhoff, N. J. (2016c). Influence of implantation on the electrochemical properties of smooth and porous TiN coatings for stimulation electrodes. J. Neural Eng. 13:026011. doi: 10.1088/1741-2560/13/2/026011

PubMed Abstract | CrossRef Full Text | Google Scholar

Meijs, S., Taylor, A., Pennisi, C. P., and Rijkhoff, N. J. M. (2013). “Electrochemical characterization of boron-doped nanocrystalline diamond electrodes for neural stimulation,” in 6th Annual International IEEE EMBS Conference on Neural Engineering (San Diego, CA).

Google Scholar

Musa, S., Rand, D. R., Cott, D. J., Loo, J., Bartic, C., Eberle, W., et al. (2012). Bottom-Up SiO 2 embedded carbon nanotube electrodes with superior performance for integration in implantable neural microsystems. ACS Nano 6, 4615–4628. doi: 10.1021/nn201609u

CrossRef Full Text | Google Scholar

Norlin, A., Pan, J., and Leygraf, C. (2005). Investigation of electrochemical behavior of stimulation sensing materials for pacemaker electrode applications. J. Electrochem. Soc. 152, J85–J92. doi: 10.1149/1.1933372

CrossRef Full Text | Google Scholar

Ohring, M. (ed.). (2002). “Substrate surfaces and thin-film nucleation,” in Materials Science of Thin Films (San Diego, CA: Academic Press), 357–415. doi: 10.1016/B978-012524975-1/50010-0

CrossRef Full Text

Petrák, V., Vlčková Živcová, Z., Krýsová, H., Frank, O., Zukal, A., Klimša, L., et al. (2017). Fabrication of porous boron-doped diamond on SiO2 fiber templates. Carbon 114, 457–464. doi: 10.1016/j.carbon.2016.12.012

CrossRef Full Text | Google Scholar

Piret, G., Hébert, C., Mazellier, J.-P., Rousseau, L., Scorsone, E., Cottance, M., et al. (2015). 3D-nanostructured boron-doped diamond for microelectrode array neural interfacing. Biomaterials 53, 173–183. doi: 10.1016/j.biomaterials.2015.02.021

PubMed Abstract | CrossRef Full Text | Google Scholar

Polini, R., Kumashiro, S., Jackson, M. J., Amar, M., Ahmed, W., and Sein, H. (2006). A study of diamond synthesis by hot filament chemical vapor deposition on Nc coatings. J. Mater. Eng. Perform. 15, 218–222. doi: 10.1361/105994906X95913

CrossRef Full Text | Google Scholar

Prawer, S., and Nemanich, R. J. (2004). Raman spectroscopy of diamond and doped diamond. Philos. Trans. A Math. Phys. Eng. Sci. 362, 2537–2565. doi: 10.1098/rsta.2004.1451

PubMed Abstract | CrossRef Full Text | Google Scholar

Qureshi, A., Gurbuz, Y., Howell, M., Kang, W. P., and Davidson, J. L. (2010). Nanocrystalline diamond film for biosensor applications. Diam. Relat. Mater. 19, 457–461. doi: 10.1016/j.diamond.2010.01.012

CrossRef Full Text | Google Scholar

Rao, T. N., and Fujishima, A. (2000). Recent advances in electrochemistry of diamond. Diam. Relat. Mater. 9, 384–389. doi: 10.1016/S0925-9635(99)00234-4

CrossRef Full Text | Google Scholar

Schwarzová-Pecková, K., Vosáhlová, J., Barek, J., Šloufová, I., Pavlova, E., Petrák, V., et al. (2017). Influence of boron content on the morphological, spectral, and electroanalytical characteristics of anodically oxidized boron-doped diamond electrodes. Electrochim. Acta 243, 170–182. doi: 10.1016/j.electacta.2017.05.006

CrossRef Full Text | Google Scholar

Siuzdak, K., Bogdanowicz, R., Sawczak, M., and Sobaszek, M. (2015). Enhanced capacitance of composite TiO 2 nanotube/boron-doped diamond electrodes studied by impedance spectroscopy. Nanoscale 7, 551–558. doi: 10.1039/C4NR04417G

CrossRef Full Text | Google Scholar

Smart, S. K., Cassady, A. I., Lu, G. Q., and Martin, D. J. (2006). The biocompatibility of carbon nanotubes. Carbon 44, 1034–1047. doi: 10.1016/j.carbon.2005.10.011

CrossRef Full Text | Google Scholar

Specht, H., Krüger, F., Wachter, H. J., Keitel, O., Leitold, C., and Frericks, M. (2006). “Electrochemical properties and stability of PVD coatings for the application in cardiac and neurological stimulation,” in Medical Device Materials III - Proceedings of the Materials and Processes for Medical Devices Conference 2005 (Boston, MA), 169–173.

Google Scholar

Subramanian, B., Muraleedharan, C. V., Ananthakumar, R., and Jayachandran, M. (2011). A comparative study of titanium nitride (TiN), titanium oxy nitride (TiON) and titanium aluminum nitride (TiAlN), as surface coatings for bio implants. Surf. Coat. Technol. 205, 5014–5020. doi: 10.1016/j.surfcoat.2011.05.004

CrossRef Full Text | Google Scholar

Suzuki, A., Ivandini, T. A., Yoshimi, K., Fujishima, A., Oyama, G., Nakazato, T., et al. (2007). Fabrication, characterization, and application of boron-doped diamond microelectrodes for in vivo dopamine detection. Anal. Chem. 79, 8608–8615. doi: 10.1021/ac071519h

PubMed Abstract | CrossRef Full Text | Google Scholar

Svítková, J., Ignat, T., Švorc, L., Labuda, J., and Barek, J. (2016). Chemical modification of boron-doped diamond electrodes for applications to biosensors and biosensing. Crit. Rev. Anal. Chem. 46, 248–256. doi: 10.1080/10408347.2015.1082125

PubMed Abstract | CrossRef Full Text | Google Scholar

Swain, G. M. (1994). The Susceptibility to surface corrosion in acidic fluoride media: a comparison of diamond, HOPG, and glassy carbon electrodes. J. Electrochem. Soc. 141, 3382. doi: 10.1149/1.2059343

CrossRef Full Text | Google Scholar

Taylor, A., Ashcheulov, P., Hubík, P., Klimša, L., Kopeček, J., Remeš, Z., et al. (2018). Precursor gas composition optimisation for large area boron doped nano-crystalline diamond growth by MW-LA-PECVD. Carbon 128, 164–171. doi: 10.1016/j.carbon.2017.11.063

CrossRef Full Text | Google Scholar

Taylor, A., Fekete, L., Hubík, P., Jäger, A., Janíček, P., Mortet, V., et al. (2014). Large area deposition of boron doped nano-crystalline diamond films at low temperatures using microwave plasma enhanced chemical vapour deposition with linear antenna delivery. Diam. Relat. Mater. 47, 27–34. doi: 10.1016/j.diamond.2014.05.002

CrossRef Full Text | Google Scholar

Trouillon, R., and O'Hare, D. (2010). Comparison of glassy carbon and boron doped diamond electrodes: resistance to biofouling. Electrochim. Acta 55, 6586–6595. doi: 10.1016/j.electacta.2010.06.016

CrossRef Full Text | Google Scholar

van Hove, R. P., Sierevelt, I. N., van Royen, B. J., and Nolte, P. A. (2015). Titanium-nitride coating of orthopaedic implants: a review of the literature. Biomed Res. Int. 2015:485975. doi: 10.1155/2015/485975

PubMed Abstract | CrossRef Full Text | Google Scholar

Vermeeren, V., Wenmackers, S., Wagner, P., and Michiels, L. (2009). DNA sensors with diamond as a promising alternative transducer material. Sensors 9, 5600–5636. doi: 10.3390/s90705600

PubMed Abstract | CrossRef Full Text | Google Scholar

Vlčková Živcová, Z., Mortet, V., Taylor, A., Zukal, A., Frank, O., and Kavan, L. (2018). Electrochemical characterization of porous boron-doped diamond prepared using SiO2 fiber template. Diam. Relat. Mater. 87, 61–69. doi: 10.1016/j.diamond.2018.05.007

CrossRef Full Text | Google Scholar

Weiser, P. S., Prawer, S., Hoffman, A., Manory, R. R., Paterson, P. J. K., and Stuart, S. (1992). Carbon diffusion in uncoated and titanium nitride coated iron substrates during microwave plasma assisted chemical vapor deposition of diamond. J. Appl. Phys. 72, 4643–4647. doi: 10.1063/1.352119

CrossRef Full Text | Google Scholar

Williams, O. A. (2011). Nanocrystalline diamond. Diam. Relat. Mater. 20, 621–640. doi: 10.1016/j.diamond.2011.02.015

CrossRef Full Text | Google Scholar

Yu, Y., Wu, L., and Zhi, J. (2014). Diamond nanowires: fabrication, structure, properties, and applications. Angew. Chem. Int. Ed. Engl. 53, 14326–14351. doi: 10.1002/anie.201310803

PubMed Abstract | CrossRef Full Text | Google Scholar

Zanin, H., May, P. W., Fermin, D. J., Plana, D., Vieira, S. M., Milne, W. I., et al. (2014). Porous boron-doped diamond/carbon nanotube electrodes. ACS Appl. Mater. Interfaces 6, 990–995. doi: 10.1021/am4044344

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, Y., and Zhi, J. (2009). The application of boron-doped diamond electrodes in amperometric biosensors. Talanta 79, 1189–1196. doi: 10.1016/j.talanta.2009.05.026

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: neural prosthesis, neural interfaces, implantable electrodes, electrical stimulation, boron-doped diamond, porous diamond, titanium nitride, electrochemistry

Citation: Meijs S, McDonald M, Sørensen S, Rechendorff K, Fekete L, Klimša L, Petrák V, Rijkhoff N, Taylor A, Nesládek M and Pennisi CP (2018) Diamond/Porous Titanium Nitride Electrodes With Superior Electrochemical Performance for Neural Interfacing. Front. Bioeng. Biotechnol. 6:171. doi: 10.3389/fbioe.2018.00171

Received: 29 June 2018; Accepted: 25 October 2018;
Published: 15 November 2018.

Edited by:

Jeffrey R. Capadona, Case Western Reserve University, United States

Reviewed by:

Diego Mantovani, Laval University, Canada
Rajendra K. Singh, Institute of Tissue Regeneration Engineering (ITREN), South Korea

Copyright © 2018 Meijs, McDonald, Sørensen, Rechendorff, Fekete, Klimša, Petrák, Rijkhoff, Taylor, Nesládek and Pennisi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Cristian P. Pennisi, cpennisi@hst.aau.dk

Present Address: Václav Petrák, Faculty of Biomedical Engineering, Department of Natural Sciences, Czech Technical University, Kladno, Czechia