An injectable hyperthermic nanofiber mesh with switchable drug release to stimulate chemotherapy potency

Introduction

Drug delivery systems (DDSs) are being widely investigated as technologies to reduce the effects of drugs on normal cells while specifically targeting cancer cells (Parisi et al., 2014; Sanadgol and Wackerlig, 2020). In a DDS, the drug distribution and concentration are controlled spatiotemporally to reduce side effects and improve drug efficacy by administering only the necessary and sufficient amount of the drug. In the study of DDSs in the treatment of cancer, the enhanced permeation and retention (EPR) effect discovered by Maeda et al. has often been applied (Maeda, 2010). The EPR effect refers to the fact that neovascularization, which occurs rapidly around cancerous tumors, has an incomplete structure with gaps between the vascular endothelial cells. By leaking into the interstitial tissues, nanoparticles with diameters of 100 nm or less are easily delivered to the tumor sites. A significant benefit of this effect is that it allows selective drug delivery to cancerous tumors (Maeda and Islam, 2020; Subhan et al., 2021). However, due to the uncontrollable nature of passive targeting, there are still some challenges to making EPR effect in clinical application.

The concept of a reservoir formulation has attracted attention as a locally controllable DDS from a different perspective than the EPR effect. A reservoir formulation is a drug-loaded formulation that is implanted in the patient’s body so that the drug can be released into the body at any time. The formulation is implanted under the skin or in an affected area of the patient’s body through surgery involving incision and removal (Sapino et al., 2019; Fayzullin et al., 2021); the aim here is to administer the necessary and sufficient amount of the drug at the appropriate time. The drug reservoir is often located near the affected area, allowing for physical space and temporal control through the timing of drug administration from the reservoir. Reservoir-based formulations have been used for patients who require long-term care and continuous drug administration, such as those with hormonal imbalances or osteoporosis (Villarruel Mendoza et al., 2020), patients undergoing chemotherapy supported by subcutaneous port implantation, and diabetic patients (Anselmo and Mitragotri, 2014; Sanjay et al., 2018; Li W et al., 2022) using insulin pump therapy. From these clinical experiences, it is clear that reservoir-based formulations can improve the quality of life by preventing missed or lost medications and reducing the physical and mental burden of patients due to long-term drug dependency. Therefore, we developed and studied a smart nanofiber mesh (SNM) as a new cancer treatment platform that enables active targeting of cancerous tumors by focusing on the concept of reservoir-based formulation delivery.

SNM is a type of nanofiber with “smart” properties, based on their physical or chemical properties such as “stimuli-responsive” and “environmental-sensitive”, which for such applications as “on-off” switchable control of swelling/deswelling and adhesion behavior (Wang et al., 2019; Wang et al., 2022). This material can be produced by electrospinning through a single needle (Khodadadi et al., 2020), coaxial electrospinning (Peng et al., 2021; Li J et al., 2022), or bubble spinning (He et al., 2022; Qian and He, 2022). The SNM is surgically implanted near the affected area and can effectively treat diseases through active targeting and long-term release of encapsulated drugs. As an example, an SNM targeted at carpal tunnel syndrome encapsulates a conventional therapeutic drug for carpal tunnel syndrome in a nanofiber mesh composed of polycaprolactone. The SNM has been successfully applied to stimulate the regeneration of peripheral nerves, which is not possible with a single therapeutic drug and is currently under clinical trials in humans (Grinsell and Keating, 2014; Hoyng et al., 2015).

In addition, a SNM for cancer therapy was developed using poly (N-isopropylacrylamide-co-N-hydroxymethyl acrylamide) (P(NIPAAm-co-HMAAm)), a temperature-responsive polymer, as the substrate and magnetic nanoparticles (MNPs) as the heating elements. HMAAm was used to provide the chemical crosslinking points as well as to adjust the phase transition temperature around hyperthermic temperature. The SNM was designed to induce phase transition of P(NIPAAm-co-HMAAm) by heat generation from the MNPs, thus enabling accelerated release of the drug. Furthermore, by maintaining the heat generation temperature around 42–43°C, we were able to combine hyperthermia, a kind of heat-based cancer therapy, with chemotherapy by releasing the encapsulated drug. We aim to develop a minimally invasive therapy with fewer side effects on normal cells through localized combined hyperthermia/chemotherapy.

As a method of providing injectability, we considered using the shear-thinning property in the SNM. The shear-thinning property is a phenomenon by which a material becomes fluid under an applied shear stress. This is known to occur when the shear stress increases the mobility of the molecules by breaking the intermolecular interactions, orienting molecules along the stress direction, and removing entanglements between molecules (Dufresne and Castaño, 2017; Sadasivuni et al., 2019). Therefore, we decided to add polyvinyl alcohol (PVA) to the substrate of the SNM to control the interactions between the methylol group of P(NIPAAm-co-HMAAm) and hydroxy group of PVA derived from hydrogen bonding (Cho et al., 2010; Nuruddin et al., 2021).

This study was aimed at applying the SNM to the affected area by injection without surgery. The injectable SNM not only enables minimally invasive implantation in the patient’s body but also has the advantage of improved handling performance. A minimally invasive SNM based on the P(NIPAAm-co-HMAAm) and PVA was developed for application by injection. This SNM responds to an alternating magnetic field (AMF) owing to the loaded MNPs and shows temperature-responsive drug release behavior (Scheme 1). Therefore, the well-defined multifaceted platform represents an appreciable SNM for efficient and minimally invasive distinctive therapy to the tumor cells, which is important in the validation of strategic pursuit of promoted combination therapeutic outcomes through hyperthermia and chemotherapeutics.

SCHEME 1

SCHEME 1. Design of a smart injectable hyperthermia nanofiber system with MNPs dispersed in temperature-responsive polymers. The nanofibers also incorporate an anticancer drug. With the device signal (AMF) turned “on” to activate the MNPs in the nanofibers, the MNPs generate heat to collapse the polymer network in the nanofiber, thus allowing “on-off” release of the drug. Following hyperthermia and chemotherapy, the heat and drug release induce apoptosis of the cancerous cells.

Results and discussion

Fabrication of smart nanofiber mesh

As shown in Scheme 2, NIPAAm and HMAAm were randomly copolymerized to form P (NIPAAm-co-HMAAm). In this study, a copolymer with a relatively high molecular weight (Mw 50 k∼) was synthesized. As a result of insufficient molecular chain entanglement during electrospinning, the lower molecular weight may cause beads or particles to form. As molecular weight increases, fiber morphology changes from beads to fine fibers. According to our previous report (Oroumei et al., 2015; Ahmadian et al., 2021), PNIPAAm with Mn = 10 k forms a bead-like structure at 1.0–10 w/v%. With PNIPAAm with a relatively higher molecular weight, fibers were electrospun on a lower solution concentration of 1.0–3.0%.

SCHEME 2

SCHEME 2. Diagrammatic illustration of the SNM with poly (NIPAAm-co-HMAAm)/PVA.

To adjust the shear-thinning property of SNM to achieve injectability, the hydrophilic ability will be introduced. Herein, the fiber fabrication conditions were optimized as follows: 25-gauge needle, 20 wt% polymer concentration, three different amounts (0, 5, 10 wt%) of PVA, 20 kV of applied voltage, and a 13 cm gap between the collector and the needle. A comparison of the isopropyl group of the NIPAAm and the methylol group of HMAAm by ¹H NMR determined that the HMAAm content in the copolymer was 20 mol%. Approximately 41–43°C was selected as a mild-hyperthermia temperature for the copolymer’s phase transition. The corresponding phase transition temperature value was 42.1°C.

Characterization of smart nanofiber mesh

In this study, poly (NIPAAm-co-HMAAm)/PVA nanofibers with different amounts of PVA were fabricated through blend electrospinning. Figure 1A shows the optical microscopy and SEM images of a variety of electrospun nanofiber meshes after thermal crosslinking. As shown in Figure 1A, the nanofiber before crosslinked were bead-free, smooth and uniform fibers were formed with an average diameter of 1,120 ± 231, 640 ± 200, 608 ± 190 nm, respectively, regardless of PVA content. It is maybe the introduced of HMAAm units is to crosslink polymer chains, and this induced the stability of the meshes in aqueous medium (Kim and Matsunaga, 2017; Niiyama et al., 2018; Zha et al., 2021). Moreover, after thermal crosslinking, the nanofibers maintained nanofibrous structures and the average diameter was 1,111 ± 110, 509 ± 118, 547 ± 250 nm, respectively (Figure 1B). These results suggest that thermal crosslinking occurs only within the fibers and does not contribute to inter-fiber adhesion.

FIGURE 1

FIGURE 1. (A) SEM image and (B) the average diameters of the nanofibers: Poly (NIPAAm- c o-HMAAm) pre-crosslinked with PVA, poly (NIPAAm- c oHMAAm) post-crosslinked with PVA and poly (NIPAAm- c o-HMAAm) crosslinked with PVA after injection. (C) Change in 1H NMR spectra of the poly (NIPAAm- c o-HMAAm)/PVA processed with different heating times.

Furthermore, it has been confirmed that the nanofiber poly (NIPAAm-co-HMAAm)/PVA has a crosslinked structure by the disappearance of peaks corresponding to methylol groups in the attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) spectra (Figure 1C). The peaks at 1,650 and 1,550 cm⁻¹ were assigned to amide I (C=O stretching) and amide II (N–H bending) of the copolymer, respectively. The broad absorption band observed around 3,430 cm⁻¹ was assigned to the N–H stretching of amide groups in the copolymer. The peaks at 1,370–1,390 and 2,980 cm⁻¹ were assigned to the respective stretching modes of NIPAAm’s–CH(CH₃)₂ and –(CH₃)₂ groups. The peaks at 1,050, 1,230, and 3,300 cm⁻¹ were assigned to the C–O–H stretching, C–O–H bending, and O–H stretching modes in HMAAm, respectively (Niiyama et al., 2018; Wei et al., 2022). With increasing thermal treatment time, the signal intensity of the methylol groups gradually decreased. It was confirmed that the signals completely disappeared and the thermal crosslinking was completed in 6 h.

Shear thinning properties of smart nanofiber mesh

In recent years, electrospun nanofibers have made rapid advances in cancer therapy due to their high drug loading rates, high specific surfaces, and good drug release profiles. However, drug-loaded nanofibers are typically applied externally or injected through fibrous membranes to treat cancer, resulting in reduced complicated surgical operations, drug utilization, and secondary damage. For the purpose of solving these bottlenecks, injectable electrospun nanofibers (PNIPHM_PVA0, PNIPHM_PVA5, and PNIPHM_PVA10) thermally crosslinked with different ratios of PVA were fabricated. For investigating the injectability of the nanofiber meshes, the strain amplitude-dependent dynamic viscoelasticity, shear rate dependent viscosity, and continuous-step strain measurement were performed.

The loss modulus of each SNM exceeded the storage modulus when the strain is applied above a certain threshold value (Figure 2A). The strain value at the point where the loss modulus first exceeded the storage modulus was 379% for PNIPHM_PVA0, while it was 200% for PNIPHM_PVA5 and PNIPHM_PVA10. This result confirms the increase of flowability with the increase rate of PVA. Furthermore, the results of shear rate dependent viscosity measurement were shown in Figure 2B. The shear-thinning property of SNM with any PVA content decreases with an increasing shear rate. The shear-thinning property of SNMs was confirmed. In addition, it can be noted that for all types of SNM, the storage modulus was higher when the shear strain was 1% and the loss modulus was higher when the shear strain was 1,000% in Figure 2C. This indicates that the mesh becomes fluid only when shear strain is applied and recovers its shape when the strain is removed. Moreover, at 1% shear strain, the storage modulus increased with time. This indicates that the nanofiber mesh absorbs water from the surrounding area after being subjected to strong shear force, and gradually recovers its shape by swelling again. The storage modulus at the beginning of the measurement (0 s) was set to 100%, and the shape recovery rate was calculated by comparing the storage modulus at 120, 300, 480, 660, and 780 s. The shape recovery rates were 103, 97, 78, 78, 109% for PNIPHM_PVA0, 128, 67, 55, 49, 38% for PNIPHM_PVA5, and 113, 50, 23, 16, 13% for PNIPHM_PVA10. It was found that the shape recovery rate worsened with the increasing PVA addition rate. A possible explanation is that the addition of PVA, a crystalline polymer, increases the crystallinity of the nanofiber mesh, which in turn increases the mechanical strength and decreases flexibility (Faris et al., 2021; Liang et al., 2021; Zhou et al., 2022). On the basis of those results, this composition (PNIPHM_PVA5) was selected for further study.

FIGURE 2

FIGURE 2. (A) Result of strain amplitude dependent dynamic viscoelasticity measurement, (B) Result of shear rate dependent viscosity measurement, and (C) Result of continuous-step strain measurement (n = 3, mean ± SD).

AMF-responsive heat generation

Thermal therapy known as magnetic hyperthermia involves heating tumors using magnetic nanoparticles and applying an AMF to create heat (Albarqi et al., 2020; Gavilan et al., 2021). High temperatures (42–45°C)can kill cancer cells, as well act as a sensitizer to enhance the effects of chemotherapy (Ahmed K, 2012). As a result of its excellent ability to penetrate tissues, AMF can precisely treat deep tumors in organs (Hayashi et al., 2016). In this regard, it is crucial to determine the heating potential of MNPs within nanofiber meshes exposed to AMF. Generally, magnetic fields affect tissues and organ systems in certain ways, such as causing induced eddy currents in tissues, resulting in necrosis or carbonization in healthy tissues. Thereby, magnetic field forces are restricted. The AMF safety frequency threshold is 100–300 kHz in clinical applications (Mamiya et al., 2017; Chen et al., 2021). In this study, an AMF of 281 kHz was used and its intensity is relevant for clinical applications.

To investigate the responses of the MNPs-loaded nanofibers to a magnetic field, Figure 3A shows the heating profiles of the SNMs with 30 wt% MNPs loading with AMF switching every 360 s (on for 300 s and off for 60 s). The temperature of the SNM increased from 23.7°C to 42.5°C caused by the irradiation of 281 kHz AMF and dropped immediately to the starting temperature once the irradiation was turned off. Furthermore, in Figure 3B, it shows the time-dependent temperature changes of the SNMs with different MNPs loading rates under AMF irradiation for 360 s. The temperature of each test sample sharply increased immediately after AMF irradiation, reaching a plateau within 30 s at 33.4°C, 42.1°C, and 47.3°C in meshes with 10 wt%, 20 wt%, and 30 wt% MNPs loading, respectively. The result indicated that the heating profiles are dependent on the concentration of MNPs loading, which will make the heating ability of the SNM more controllable. Based on these results, the SNM with 30% (9 mg) of MNPs was chosen for further investigation.

FIGURE 3

FIGURE 3. (A) Infrared thermal images of MNPs-loaded SNM with “on” or “off” AMF irradiation. (B) Heating profiles of MNPs@SNM with different MNPs contents during AMF application at different times.

“On-off” switchable drug release

Controllable drug release is an important evaluation criterion for SNM. Next, the nanofiber meshes containing PTX, MNPs and PVA were used to verify the drug release behavior from nanofiber. NIPAAm-co-HMAAm polymer is soluble in aqueous media below LCST and dehydrates rapidly above LCST. Therefore, PTX is released from the polymer along with the heating of MNPs induced by AMF. Figure 4 shows the switching from “on” to “off” AMF applications on PTX release from nanofibers. It is worth noting that approximately 16% of loaded PTX were released in the first heating process. However, the release was stopped after cooling to room temperature, and restarted release during the second heating. It is demonstrated that the release of the PTX was accelerated by AMF irradiation and was stopped at room temperature. Interestingly, with the increase of the switching cycle number, the cumulative release of PTX also increased significantly, and showed a switching cycle-dependent relationship. The release of the PTX cumulative amount was over 70% within eight cycles. Owing to the high specific surface area of nanofibers, their sensitivity to external stimuli is greater than that of bulk materials (Lin et al., 2013). Thus, we took advantage of the nanometric effects of nanofibers for the “on-off” switchable release of drugs. From this regard, these results in Figure 4 indicate that the proposed SNM system allows the control of switchable drug release by simply switching the AMF “on” and “off,” and this controlled release SNM may be very promising for cancer therapeutics in the future.

FIGURE 4

FIGURE 4. Drug release behavior from SNM containing PTX (1.0 wt%), MNPs (0 or 20 wt%) and PVA (5 wt%) at different cycles of AFM.

Anti-tumor effect

Hybrid nanoarchitectures with magnetic nanoparticles have been used for hyperthermic cancer cell therapy in recent years (Lin et al., 2013). However, due to their nano dimensional properties, these hybrids cannot be directly applied to living matter, as they may lead to toxic side effects. Interestingly, one of the advantages of the SNM in this study is that a nanofiber as a dressing can be manipulated and injected directly into the tumor region. In this regard, the anti-tumor effects of the SNMs based on hyperthermia and the chemotherapeutic effects of the MNPs/PTX@SNM was investigated using SKOV3 cells.

Before co-culturing with nanofiber mesh the cell viability of SKOV3 cells treated with PTX was assessed by the alamar Blue assay. A culture medium containing PTX was used to culture the SKOV3 cells at 0, 0.001, 0.1, 1.0, and 10 μg ml⁻¹ concentration for 24 h. As shown in Figure 5A, cell viability decreases as the drug concentration increases, and IC₅₀ of SKOV3 at 1.66 μg ml⁻¹. Furthermore, the anti-tumor effects of the SNMs were further evaluated using SKOV3 cells. The cells were co-cultured with the SNMs for 24 h And the cells were exposed to AMF once every 5 min and then at room temperature for 5 min for each cycle. As demonstrated in Figure 5B, the blank SNM under the AMF maintained cell viability higher than 95%, indicating that the blank nanofiber mesh and magnetic field was non-toxic against SKOV3 cells. On the other hand, with the SNM exposure to AMF, the cell viability of SNM loaded with MNPs alone (MNPs-SNM) and PTX alone (PTX@SNM) decreased gradually compared to the control group (medium only). This may be due to the effect of hyperthermia generated by MNPs-loaded SNM exposed to AMF and chemotherapy effect resulting from PTX release from surface of nanofiber. Moreover, the cells viability in the presence of MNPs and PTX co-loaded nanofibers group was 60.45 ± 6.72. This result may be due to few PTX being released from the surface of nanofibers without AMF application (“off” state). This result corresponds well to the in vitro drug release study (Figure 4). It can be noted that, the cell viability of MNPs/PTX@SNM with the magnetic field was only 11.23% ± 5.01, because of the combined effect of hyperthermia with PTX released caused by heating. In sum, the findings demonstrate that MNPs and PTX@SNM exposed to AMF greatly increase cytotoxicity in SKOV3 cells. In light of this, this platform may be developed into an implantable delivery system for treating ovarian cancer.

FIGURE 5

FIGURE 5. Cell viability of SKOV3 cells treated with (A) different concentrations of PTX, and (B) nanofiber meshes with different cycles of AFM. (Data are mean ± SD n = 6, **p < 0.01).

Experimental

Materials

Dimethylformamide (DMF), 2′2-Azobis (isobutyronitrile) (AIBN) and N-Isopropylacrylamide (NIPAAm) were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). The following products were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan): N-Hydroxymethylacrylamide (HMAAm), 1,1,1,3,3,3-Hexafluoro-2-propanol, and Paclitaxel (PTX). We obtained ferrofluid made of iron oxide (III) (10 nm particle size) from Ferrotec Holdings Corporation (Tokyo, Japan). Fetal bovine serum (FBS) was purchased from Tocris Bioscience Inc. (Minneapolis, MN, United States). McCoy’s 5 A Medium Modified, content sodium bicarbonate, free of L-glutamine, liquid, and sterile-filtered for cell culture was purchased from Sigma-Aldrich Japan (Tokyo, Japan). We obtained the Alamar Blue reagent from TREK Diagnostic Systems (Cleveland, OH, United States). The American Type Culture Collection (Manassas, VA, United States) provided SKOV3 (human ovarian cancer cell line: adenocarcinoma).

Synthesis and characterization of P(NIPAAm-co-HMAAm)

According to the previous description, NIPAAm and HMAAm were copolymerized. Briefly, 80 mol% NIPAAm was dissolved in 20 ml of DMF, followed by 20 mol% HMAAm and 0.01 mol% AIBN. In total, the monomers possessed a molar concentration of 50 mmol. For the copolymerization, 62°C was maintained for 20 h before four freeze-thaw cycles degassed it completely. After polymerization, AIBN, unreacted monomers, impurities, and solvent were removed by dialysis against ethanol (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and distilled water for 7 days. It takes 3 days for the dialyzed solutions to be lyophilized after they have been dialyzed. The chemical structure of the copolymer was verified by NMR (JEOL, Tokyo, Japan). The average molecular weight (Mn) and polydispersity index (PDI) of the copolymer was determined by gel permeation chromatography (GPC, JASCO International, Tokyo, Japan) using DMF with lithium bromide (LiBr, 10 mm) (Tosho Corporation, Tokyo, Japan) as an eluent sample. A UV-Visible spectrophotometer (JASCO Corporation, Tokyo, Japan) with a heating rate of 1.0 C/min was used to measure temperature-dependent changes in the transmittance of the copolymer in phosphate-buffered saline (PBS) (pH = 7.4, 0.1% w/v). We defined the lower critical solution temperature of the copolymer as the temperature at which 50% of the transmission was achieved.

Fabrication of fiber meshes

A solution for electrospinning was prepared by dissolving poly (NIPAAm-co-HMAAm) in HFIP (20 w/v%). The electrospun solution contained 30 w/w%, 1.0 w/w%, 0–20 w/w% of MNPs, PTX, and polyvinyl alcohol, respectively. The solution was electrospun into fibers using an applied voltage of 20 kV with a flow rate set to 1.0 ml/h, and 13 cm separation of the needle (25 gauge) and collector plate at 25°C and 42% humidity (Nanon-01A, MECC Co., Ltd, Fukuoka, Japan). In order to remove organic solvents from electrospun fibers, they were placed in an oven (Tokyo Rikakikai Co., Ltd, Tokyo, Japan) at 140°C for 24 h. This process involved the methylal group in HMAAm and the hydroxy group in PVA were crosslinked in the nanofiber mesh. The morphology of fibers was observed using a scanning electron microscope (SEM) (SU8000, Hitachi High-Technologies Corporation, Tokyo, Japan) with secondary electrons (SE) after Pt coating of the fiber surface. The diameter of fibers was calculated from a SEM image using ImageJ software. Crosslinking between methylal groups of HMAAm within the fiber was confirmed based on the disappearance of absorbance at 1,050 cm⁻¹ through ATR-FTIR spectroscopy (IRPrestige-21, Shimadzu, Kyoto, Japan). Analysis was conducted after the residual solvent was removed from fibers by vacuum drying (ULVAC KIKO, Inc, Miyazaki, Japan).

Rheological characterization of smart nanofiber mesh

The rheological properties were tested using a rheometer (MCR301, Anton Paar Japan K.K, Tokyo, Japan). Strain amplitude-dependent dynamic viscoelasticity, shear-rate-dependent viscosity, and continuous-step strain measurement were performed. In each measurement, the prepared SNM was swollen with distilled water and then cut into 1 cm diameter circles for measurement. In the strain amplitude-dependent dynamic viscoelasticity measurement, the angular frequency was fixed at 10 rad/s, the shear strain was varied logarithmically from 0.01% to 2000%, and the storage modulus G’ (Pa) and loss modulus G” (Pa) were measured. The number of measurement points was 32, and the total measurement time was set to 96 s. In the shear-rate-dependent viscosity measurement, shear stress (Pa) was measured by varying the shear rate logarithmically from 0.001 s⁻¹ to 100 s⁻¹. The number of measuring points was 26 and the total measurement time was set to 143 s. In the continuous-step strain measurement, the angular frequency was fixed at 10 rad/s. The shear strain was repeated for 120 s at 1% and for 60 s at 1,000% for a total of four cycles, and the storage modulus G’ (Pa) and loss modulus G” (Pa) were measured. The total number of measurement points was 72, and the total measurement time was 720 s.

Nanofiber smart mesh heating profiles

MNPs within the nanofiber mesh were studied by applying AMF to determine their heat-generating properties. SNMs (6.0, 9.0, 12.0 mg) of MNPs were placed in the center of HOTSHOT2 (Alonics Co., Ltd, Tokyo, Japan) of a customized copper coil that generated AMF (480 A, 281 kHz frequency, 362 W). We measured the heating profiles at 30 and 60 s using an FL-IR thermo-camera (CPA-E6, FLIR Systems Japan K.K, Tokyo, Japan).

Drug release from smart nanofiber mesh

In 1.0 ml of phosphate-buffered saline (PBS) at room temperature, samples of SNM (30 mg) thermo crosslinked with 5% PVA were first swollen for 5 min by shaking (at 100 rpm) (Lin et al., 2019). As soon as the sample reached equilibrium, it was placed in the center of a copper coil. Afterward, 0.25 ml of the supernatant was collected, and fresh PBS was added to the supernatant that had been irradiated with AMF or cooled for 5 min at room temperature.

PTX release profiles were quantified by UV-visible spectroscopy during each switching cycle. (V-650 spectrophotometer, Jasco, Tokyo. Japan). Eight cycles were repeated. A cumulative release of PTX was calculated using the following equation: $W_n=W_{n-1}+\left\{C_n-C_{n-1}\left(1-l\right)\right\}$ , where W_n (μg) and C_n (μg ml-1) are the cumulative release amount and concentration of PTX at the n th (n = 1–16) collection process, respectively, and l is the volume collected PBS (=0.25 ml).

Cell preparation

The cells used in this study were SKOV3 (human ovarian adenocarcinoma). McCoy’s 5 A medium was used as a growth medium with 10% fetal bovine serum and 1% penicillin-streptomycin. To prepare SKOV3 cells, subcultures in a tissue culture dish (100 mm) were incubated for 2 days at 37°C in 5% CO₂ (MCO-170AICUVH, PHC Holdings, Tokyo, Japan). The following experiments collected the cells from a confluent monolayer in the dish with 0.25% (w/v) trypsin and resuspended them in 10 ml of growth medium.

Drug sensitivity of SKOV3 cells

Approximately 1.0 × 10⁵ SKOV3 cells were seeded on a 96-well plate, 200 µL of the growth medium was added and incubated for 24 h. The medium was exchanged for 96-well plates which contained 0, 0.001, 0.01, 0.1, 1.0, and 10 μg ml⁻¹ of PTX, and incubated for 24 h. After adding Alamar Blue (ten percent of the medium), each well was incubated at 37°C for 4 hours. The number of cells was calculated by measuring the fluorescent intensity at 570 nm excitation and 600 nm emission (Tecan Japan). Cell viability and IC₅₀ were calculated based on considering the number of cells in the control group (without PTX treatment) as 100% viability.

AMF-responsive anti-tumor activity In vitro

For the anticancer experiment of the SNM, SKOV3 cells were seeded in a 6 mm plate at 2 × 10⁶ cells per well for 24 h. Thereafter, the cells incubated a piece of nanofiber mesh cross-linked with 5% of PVA (which had a 30 mg weight, 30% MNPs and 1% PTX), which was exposed to AMF irradiation (480 A, amplitude 281 kHz frequency) for 5 min. After further incubation at 37°C for another 24 h, the Alamar Blue assay reagent (10% against medium) was added to each well and incubated for 4 hours at 37°C, according to protocol. The cell number was computed from the fluorescent intensity measured by a fluorescence plate drive set at 570 nm excitation and 600 nm emission.

Statistical analysis

All experiments were conducted three times and the data are presented as means ± standard deviation (SD). Statistical analysis was performed using the student t-test and variance one-way analysis (ANOVA) by Origin version 9.0 (Northampton, United States). The difference between the results was considered to be statistically significant for p < 0.01 (**).

Conclusion

In the current research, switchable drug release injectable nanofiber platforms were engineered for simultaneous for chemotherapeutic and hyperthermia to cancerous cells. The SNM were fabricated by an electrospinning method with a temperature-responsive polymer, MNPs, and PTX, and the fiber was further cross-linked with PVA. Nanofibers responded to alternating “on”, and “off” switches of AMF application and the controllable release of the drug from the fibers was observed as a result. Aiming for amplification of chemotherapeutic potency, MNPs-loaded SNM schemed. The SNM exposure to AMF enables switchable drug release and generates hyperthermia effects, which causes heat-induced cell killing as well as enhanced PTX chemotherapeutic efficiency. Subsequent investigations approved the validity of our strategic hyperthermia in amplifying chemotherapeutic potency; namely, the SNM efficiently induced apoptosis of SKOV3 cells through the synergistic anticancer effect arising from hyperthermia and PTX. Hence, the current research not only supported an intelligent nanofiber platform for injectable and controllable drug release to be fabricated, but also urged the promising potential of exploiting combination strategy in advancing tumor therapy.

Data availability statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.

Author contributions

Eexperimental preparation: MT and LC; data analysis: LC and MT; chart production: LC and MT; writing-original draft preparation: LC and MT; writing-review and editing: LC and ME. All authors have read and agreed to the published version of the manuscript.

Funding

This study was support by the JSPS KAKENHI Grant-in-Aid for Scientific Research(B) (JP19H04476) and Grant-in-Aid for Transformative Research Areas(A) (JP20H05877).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

Ahmadian, A., Shafiee, A., Aliahmad, N., and Agarwal, M. (2021). Overview of nano-fiber mats fabrication via electrospinning and morphology analysis. Textiles 1 (2), 206–226. doi:10.3390/textiles1020010

CrossRef Full Text | Google Scholar

Ahmed, K. Z. S. F., and Zaidi, S. F. (2012). Treating cancer with heat: hyperthermia as promising strategy to enhance apoptosis. J. Pak. Med. Assoc. 63 (4), 504–508.

PubMed Abstract | Google Scholar

Albarqi, H. A., Demessie, A. A., Sabei, F. Y., Moses, A. S., Hansen, M. N., Dhagat, P., et al. (2020). Systemically delivered magnetic hyperthermia for prostate cancer treatment. Pharmaceutics 12 (11), 1020. doi:10.3390/pharmaceutics12111020

PubMed Abstract | CrossRef Full Text | Google Scholar

Anselmo, A. C., and Mitragotri, S. (2014). An overview of clinical and commercial impact of drug delivery systems. J. Control. Release 190, 15–28. doi:10.1016/j.jconrel.2014.03.053

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, L., Fujisawa, N., Takanohashi, M., Najmina, M., Uto, K., and Ebara, M. (2021). A smart hyperthermia nanofiber-platform-enabled sustained release of doxorubicin and 17AAG for synergistic cancer therapy. Int. J. Mol. Sci. 22 (5), 2542. doi:10.3390/ijms22052542

PubMed Abstract | CrossRef Full Text | Google Scholar

Cho, D., Nnadi, O., Netravali, A., and Joo, Y. L. (2010). Electrospun hybrid soy protein/PVA fibers. Macromol. Mat. Eng. 295 (8), 763–773. doi:10.1002/mame.201000161

CrossRef Full Text | Google Scholar

Dufresne, A., and Castaño, J. (2017). Polysaccharide nanomaterial reinforced starch nanocomposites: a review. Starch - Starke 69 (1-2), 1500307. doi:10.1002/star.201500307

CrossRef Full Text | Google Scholar

Faris, D., Hadi, N. J., and Habeeb, S. A. (2021). Effect of rheological properties of (poly vinyl alcohol/dextrin/naproxen) emulsion on the performance of drug encapsulated nanofibers. Mater. Today Proc. 42, 2725–2732. doi:10.1016/j.matpr.2020.12.712

CrossRef Full Text | Google Scholar

Fayzullin, A., Bakulina, A., Mikaelyan, K., Shekhter, A., and Guller, A. (2021). Implantable drug delivery systems and foreign body reaction: traversing the current clinical landscape. Bioeng. (Basel) 8 (12), 205. doi:10.3390/bioengineering8120205

PubMed Abstract | CrossRef Full Text | Google Scholar

Gavilan, H., Avugadda, S. K., Fernandez-Cabada, T., Soni, N., Cassani, M., Mai, B. T., et al. (2021). Magnetic nanoparticles and clusters for magnetic hyperthermia: optimizing their heat performance and developing combinatorial therapies to tackle cancer. Chem. Soc. Rev. 50 (20), 11614–11667. doi:10.1039/d1cs00427a

PubMed Abstract | CrossRef Full Text | Google Scholar

Grinsell, D., and Keating, C. P. (2014). Peripheral nerve reconstruction after injury: a review of clinical and experimental therapies. BioMed Res. Int. 20 (14), 1–13. doi:10.1155/2014/698256

PubMed Abstract | CrossRef Full Text | Google Scholar

Hayashi, K., Sakamoto, W., and Yogo, T. (2016). Smart ferrofluid with quick gel transformation in tumors for mri-guided local magnetic thermochemotherapy. Adv. Funct. Mat. 26 (11), 1708–1718. doi:10.1002/adfm.201504215

CrossRef Full Text | Google Scholar

He, J.-H., Qian, M. Y., and Li, Y. (2022). The maximal wrinkle angle during the bubble collapse and its application to the bubble electrospinning. Front. Mat. 8 (2), 626–629. doi:10.3389/fmats.2021.800567

CrossRef Full Text | Google Scholar

Hoyng, S. A., de Winter, F., Tannemaat, M. R., Blits, B., Malessy, M. J., and Verhaagen, J. (2015). Gene therapy and peripheral nerve repair: a perspective. Front. Mol. Neurosci. 8 (13), 32. doi:10.3389/fnmol.2015.00032

PubMed Abstract | CrossRef Full Text | Google Scholar

Khodadadi, M., Alijani, S., Montazeri, M., Esmaeilizadeh, N., Sadeghi‐Soureh, S., and Pilehvar‐Soltanahmadi, Y. (2020). Recent advances in electrospun nanofiber‐mediated drugdelivery strategies for localized cancer chemotherapy. J. Biomed. Mat. Res. A 108 (7), 1444–1458. doi:10.1002/jbm.a.36912

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, Y. J., and Matsunaga, Y. T. (2017). Thermo-responsive polymers and their application as smart biomaterials. J. Mat. Chem. B 5 (23), 4307–4321. doi:10.1039/c7tb00157f

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, J., Liu, Y., and Abdelhakim, H. E. (2022). Drug delivery applications of coaxial electrospun nanofibres in cancer therapy. Molecules 27 (6), 1803. doi:10.3390/molecules27061803

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, W., Tang, J., Lee, D., Tice, T. R., Schwendeman, S. P., and Prausnitz, M. R. (2022). Clinical translation of long-acting drug delivery formulations. Nat. Rev. Mat. 7 (5), 406–420. doi:10.1038/s41578-021-00405-w

CrossRef Full Text | Google Scholar

Liang, Q., Pan, W., and Gao, Q. (2021). Preparation of carboxymethyl starch/polyvinyl-alcohol electrospun composite nanofibers from a green approach. Int. J. Biol. Macromol. 190 (3), 601–606. doi:10.1016/j.ijbiomac.2021.09.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, L., Li, H., and Liu, Y. (2019). Release oscillation in a hollow fiber – Part 2: The effect of its frequency on ions release and experimental verification. J. Low Freq. Noise, Vib. Act. Control 40 (2), 1067–1071. doi:10.1177/1461348419874973

CrossRef Full Text | Google Scholar

Lin, T. C., Lin, F. H., and Lin, J. C. (2013). In vitro characterization of magnetic electrospun IDA-grafted chitosan nanofiber composite for hyperthermic tumor cell treatment. J. Biomaterials Sci. Polym. Ed. 24 (9), 1152–1163. doi:10.1080/09205063.2012.743061

PubMed Abstract | CrossRef Full Text | Google Scholar

Maeda, H. (2010). Tumor-selective delivery of macromolecular drugs via the epr effect: background and future prospects. Bioconjug. Chem. 21 (3), 797–802. doi:10.1021/bc100070g

PubMed Abstract | CrossRef Full Text | Google Scholar

Maeda, H., and Islam, W. (2020). Overcoming barriers for tumor-targeted drug delivery. Polymer-Protein Conjug. 8, 41–58. doi:10.1016/b978-0-444-64081-9.00003-6

CrossRef Full Text | Google Scholar

Mamiya, H., Takeda, Y., Naka, T., Kawazoe, N., Chen, G., and Jeyadevan, B. (2017). Practical solution for effective whole-body magnetic fluid hyperthermia treatment. J. Nanomater. 2017, 1–7. doi:10.1155/2017/1047697

CrossRef Full Text | Google Scholar

Niiyama, E., Uto, K., Lee, C. M., Sakura, K., and Ebara, M. (2018). Alternating magnetic field-triggered switchable nanofiber mesh for cancer thermo-chemotherapy. Polym. (Basel) 10 (9), 1018. doi:10.3390/polym10091018

PubMed Abstract | CrossRef Full Text | Google Scholar

Nuruddin, M., Chowdhury, R. A., Szeto, R., Howarter, J. A., Erk, K. A., Szczepanski, C. R., et al. (2021). Structure-property relationship of cellulose nanocrystal-polyvinyl alcohol thin films for high barrier coating applications. ACS Appl. Mat. Interfaces 13 (10), 12472–12482. doi:10.1021/acsami.0c21525

PubMed Abstract | CrossRef Full Text | Google Scholar

Oroumei, A., Fox, B., and Naebe, M. (2015). Thermal and rheological characteristics of biobased carbon fiber precursor derived from low molecular weight organosolv lignin. ACS Sustain. Chem. Eng. 3 (4), 758–769. doi:10.1021/acssuschemeng.5b00097

CrossRef Full Text | Google Scholar

Parisi, O. I., Morelli, C., Puoci, F., Saturnino, C., Caruso, A., Sisci, D., et al. (2014). Magnetic molecularly imprinted polymers (MMIPs) for carbazole derivative release in targeted cancer therapy. J. Mat. Chem. B 2 (38), 6619–6625. doi:10.1039/c4tb00607k

PubMed Abstract | CrossRef Full Text | Google Scholar

Peng, W., Ren, S., Zhang, Y., Fan, R., Zhou, Y., Li, L., et al. (2021). MgO Nanoparticles-incorporated pcl/gelatin-derived coaxial electrospinning nanocellulose membranes for periodontal tissue regeneration. Front. Bioeng. Biotechnol. 9 (18), 668428–668441. doi:10.3389/fbioe.2021.668428

PubMed Abstract | CrossRef Full Text | Google Scholar

Qian, M.-Y., and He, J.-H. (2022). Collection of polymer bubble as a nanoscale membrane. Surfaces Interfaces 28, 101665. doi:10.1016/j.surfin.2021.101665

CrossRef Full Text | Google Scholar

Sadasivuni, K. K., Saha, P., Adhikari, J., Deshmukh, K., Ahamed, M. B., and Cabibihan, J. J. (2019). Recent advances in mechanical properties of biopolymer composites: a review. Polym. Compos. 41 (1), 32–59. doi:10.1002/pc.25356

CrossRef Full Text | Google Scholar

Sanadgol, N., and Wackerlig, J. (2020). Developments of smart drug-delivery systems based on magnetic molecularly imprinted polymers for targeted cancer therapy: a short review. Pharmaceutics 12 (9), 831–862. doi:10.3390/pharmaceutics12090831

PubMed Abstract | CrossRef Full Text | Google Scholar

Sanjay, S. T., Zhou, W., Dou, M., Tavakoli, H., Ma, L., Xu, F., et al. (2018). Recent advances of controlled drug delivery using microfluidic platforms. Adv. Drug Deliv. Rev. 128 (18), 3–28. doi:10.1016/j.addr.2017.09.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Sapino, S., Chirio, D., Peira, E., Abellan Rubio, E., Brunella, V., Jadhav, S. A., et al. (2019). Ocular drug delivery: a special focus on the thermosensitive approach. Nanomater. (Basel) 9 (6), 884–903. doi:10.3390/nano9060884

PubMed Abstract | CrossRef Full Text | Google Scholar

Subhan, M. A., Yalamarty, S. S. K., Filipczak, N., Parveen, F., and Torchilin, V. P. (2021). Recent advances in tumor targeting via EPR effect for cancer treatment. J. Pers. Med. 11 (6), 571–598. doi:10.3390/jpm11060571

PubMed Abstract | CrossRef Full Text | Google Scholar

Villarruel Mendoza, L. A., Scilletta, N. A., Bellino, M. G., Desimone, M. F., and Catalano, P. N. (2020). Recent advances in micro-electro-mechanical devices for controlled drug release applications. Front. Bioeng. Biotechnol. 8, 827–855. doi:10.3389/fbioe.2020.00827

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, C., Xu, L., Liu, G., Ren, Y., Lv, J., Gao, D., et al. (2019). Smart adhesion by surface treatment experimental and theoretical insights. Therm. Sci. 23 (4), 2355–2363. doi:10.2298/tsci1904355w

CrossRef Full Text | Google Scholar

Wang, Q., He, J. H., and Liu, Z. (2022). Intelligent nanomaterials for solar energy harvesting: from polar bear hairs to unsmooth nanofiber fabrication. Front. Bioeng. Biotechnol. 10, 926253–926259. doi:10.3389/fbioe.2022.926253

PubMed Abstract | CrossRef Full Text | Google Scholar

Wei, W., Zarghami, N., Abasi, M., Ertas, Y. N., and Pilehvar, Y. (2022). Implantable magnetic nanofibers with ON-OFF switchable release of curcumin for possible local hyperthermic chemotherapy of melanoma. J. Biomed. Mat. Res. A 110 (4), 851–860. doi:10.1002/jbm.a.37333

PubMed Abstract | CrossRef Full Text | Google Scholar

Zha, J.-W., Zheng, M.-S., Fan, B.-H., and Dang, Z. M. (2021). Polymer-based dielectrics with high permittivity for electric energy storage: A review. Nano Energy 89, 106438–106513. doi:10.1016/j.nanoen.2021.106438

CrossRef Full Text | Google Scholar

Zhou, Z., Yao, Y., Zhang, J., Shen, L., Xu, H., Liu, J., et al. (2022). Effects of poly(vinyl alcohol) (PVA) concentration on rheological behavior of TEMPO-mediated oxidized cellulose nanofiber/PVA suspensions. Cellulose 29 (15), 8255–8263. doi:10.1007/s10570-022-04786-1

CrossRef Full Text | Google Scholar