Mn3+/Mn4+ ion-doped carbon dots as fenton-like catalysts for fluorescence dual-signal detection of dopamine

Introduction

Up to now, the research on brain science has become more and more attractive in elucidating the structure and function of the human brain, as well as human behavior and mental activity, which involves enhancing human neural activity and improving the level of prevention, diagnosis, and treatment services for nervous system diseases (Poo and Tan, 2016). Dopamine (DA), a neurotransmitter, plays a crucial role in mood, sleep, memory, endocrine regulation, and movement (He et al., 2020; Wang et al., 2021). The level of DA in the brain of healthy people ranges from 1.3 to 2.6 μmol, and abnormal DA levels often lead to various diseases, including anorexia, epilepsy, Alzheimer’s disease, schizophrenia, and attention deficit dysfunction (Chen and Zhou, 2015; He et al., 2018). In addition, DA has been widely used in cell interfaces, drug delivery, biosensing coatings, and antibacterial research due to its rich amino and catechin functional groups on its surface (Natan et al., 2019). Therefore, considering the importance of DA in neurotransmission, drug delivery, and disease treatment, it is crucial to determine the concentration of DA in vitro.

To date, many methods for detecting DA have been developed to speed up the diagnosis and prevention of DA-related diseases to some extent, such as high-performance liquid chromatography (HPLC), electrochemistry, etc (Ngo et al., 2017; Du et al., 2019; Zhao et al., 2021). However, these methods usually have the disadvantages of expensive instruments and equipment, professional technology, and long time-consuming, which limit the broad application of the above-mentioned DA detection (Zhao et al., 2021). In contrast, the fluorescence detection method has the advantages of high sensitivity, convenient instruments, and low cost, which overcomes the methods above-mentioned for detecting DA (Du et al., 2019; Wang J. G et al., 2019). In recent years, researchers have developed a variety of fluorescent materials, including AIE dyes, upconversion nanoparticles, fluorescent metal nanoclusters, carbon dots (CDs), and other fluorescent materials (Wang et al., 2010; Tu and Wang, 2013; Yang et al., 2015; Anjali Devi et al., 2017; Luo et al., 2019). Therein, CDs are widely used in biosensing and bioimaging due to their excellent photostability and biocompatibility (Xu et al., 2021; Liu et al., 2013; He et al., 2018). For example, Xu et al. constructed a fluorescence resonance energy transfer (FRET) detection platform based on the synthesized yellow fluorescent CDs. This platform had high selectivity and sensitivity, and could specifically detect l-threonine in samples with the detection range of 0.1–0.5 mM (Xu et al., 2021). Based on the FRET effect, Liang et al. prepared a novel hybrid proportional fluorescence probe of CDs-gold nanoclusters that could detect DA in the 5–180 nM range (He et al., 2018). Zhou et al. synthesized long-wavelength emission fluorescent CDs by the one-pot hydrothermal method. The prepared CDs showed bright red fluorescence in different states, which could be used for diagnostic imaging of precious metal ions in vivo and in vitro (Gao et al., 2018). However, to the best of our investigation literature, the specific recognition of DA by Mn³⁺/Mn⁴⁺ ion-doped CDs as a dual-signal fluorescence sensing platform has not been reported.

As shown in Scheme 1, manganese acetate and O-phenylenediamine were used as manganese and carbon sources to design synthesis Mn³⁺/Mn⁴⁺ ion-doped CDs by hydrothermal method. DA is an important organic chemical in the catechol family that plays an important role in human motor, neuroendocrine regulation, disease diagnosis, and drug delivery. However, clinical DA is often replaced with DA hydrochloride and hydrogen bromide due to its susceptibility to oxidation in the air and light. Mn³⁺/Mn⁴⁺ ion-doped CDs had good optical stability and Fenton-like catalytic properties; and could detect DA with dual-response fluorescence with linear ranges of 100–275 nM and 325–525 nM, and detection limits of 3 and 12 nM, respectively. Therefore, because of its wide linear range and low detection limit, it has a broad application prospect in diagnosing and preventing DA-related diseases. In addition, the material has a good role and effect in the detection of oxidation/reduction biomolecules or metal ions, and can be widely used in the detection and diagnosis caused by abnormalities of related biomolecules or metal ions.

SCHEME 1

SCHEME 1. Schematic diagram of Mn³⁺/Mn⁴⁺ ion-doped CDs preparation and its application in DA detection.

Experimental section

Materials

O-phenylenediamine, ascorbic acid (AA), glycine (Gly), glutathione (GSH), dopamine (DA), iron chloride, bismuth chloride, hafnium chloride, hydrochloric acid, indium chloride, and lysine (Lys) were obtained from Aladdin Reagent Co., Ltd. Sulfamic acid (SA), manganese acetate, NaCl, copper chloride, manganese chloride, N, N-dimethylformamide (DMF), and NaOH were gained from Tianjin Guangfu Fine Chemicals Co., Ltd. The ultrapure water in the whole experiment was prepared by the BK-10B system.

Characterization

The particle size of Mn³⁺/Mn⁴⁺ ion-doped CDs was recorded by a JEM-2100 transmission electron microscope (TEM). The height distribution of Mn³⁺/Mn⁴⁺ ion-doped CD was detected by atomic force microscopy (AFM). The UV-vis absorption and fluorescence spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs were analyzed by an FS5 spectrophotometer. Fourier transform infrared (FT-IR) spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs were measured by Bruker spectrometer. The structure of Mn³⁺/Mn⁴⁺ ion-doped CDs was recorded by X-ray diffraction (XRD) using Cu Kα radiation at a voltage of 40 kV and current of 40 mA. X-ray photoelectron spectroscopy (XPS) of Mn³⁺/Mn⁴⁺ ion-doped CDs was obtained by ESCALAB 250 Xi electron spectrometer.

Synthesis of Mn³⁺/Mn⁴⁺ ion-doped CDs

During the stirring process, 0.31 g O-phenylenediamine and 0.331 g manganese acetate were dissolved in 8 ml DMF solution (containing 1 ml (1%) acetic acid) to form a mixed solution. The above-mixed solution was transferred to a 25 ml reaction vessel and heated at 200°C for 12 h. Subsequently, the solution was centrifuged to remove the solid precipitates at room temperature. Finally, solid Mn³⁺/Mn⁴⁺ ion-doped CDs were obtained by rotation.

Detection of DA using Mn³⁺/Mn⁴⁺ ion-doped CDs

Add different contents of DA (0–0.55 μM) aqueous solution to 3 ml of Mn³⁺/Mn⁴⁺ ion-doped CDs. The fluorescence spectra were then measured at 380 nm excitation. Selectivity was achieved by adding other biomolecules (including SA, Gly, GSH, AA, Bi³⁺, Cu²⁺, Hf⁴⁺, Fe³⁺, In³⁺, Mn²⁺, Na⁺, and Lys) instead of DA. The competitive experiments were carried out by adding DA to the Mn³⁺/Mn⁴⁺ ion-doped CDs solution.

Results and discussion

Characterization of Mn³⁺/Mn⁴⁺ ion-doped CDs

The structure, morphology, and optical properties of Mn³⁺/Mn⁴⁺ ion-doped CDs were studied under heating at 200°C for 12 h. The TEM image of Mn³⁺/Mn⁴⁺ ion-doped CDs was shown in Figures 1A,B, and the as-prepared CDs exhibited good dispersion and uniform size of quasi-spherical shape with an average particle size of 3.5 nm (Figure 1B). Furthermore, the HRTEM in the inset of Figure 1A showed that the lattice spacing of the Mn³⁺/Mn⁴⁺ ion-doped CDs was 0.21 nm, corresponding to the (002) crystal plane of graphite carbon (Tang et al., 2018). In addition, as shown in Figure 1C, Supplementary Figures S1, S2, the height and morphology of Mn³⁺/Mn⁴⁺ ion-doped CDs were further studied by AFM. The AFM image showed that the height of Mn³⁺/Mn⁴⁺ ion-doped CDs was about 2.0 nm (Supplementary Figures S1, S2), similar to the TEM results. The X-ray diffraction (XRD) pattern of Mn³⁺/Mn⁴⁺ ion-doped CDs was shown in Figure 1D. A new wide diffraction peak appears in the XRD pattern of the CDs, located at 26.8°C, which is related to the (002) crystal plane of the graphitic carbon material (Tian et al., 2017; Gao et al., 2018). The aqueous solution of Mn³⁺/Mn⁴⁺ ion-doped CDs showed two UV-vis absorption peaks at 270 and 434 nm, as shown in Figure 1E. The absorption peak at 270 nm was attributed to the π-π conjugation of the benzene ring, which exhibited a lower energy absorption band at approximately 434 nm (Shi et al., 2020). The fluorescence emission spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs are shown in Figure 1F. It can be seen that the fluorescence emission peak moved in the direction of a long wavelength, and the fluorescence intensity changed with the increase of the excitation wavelength. The illustration in Figure 1F shows the fluorescent color of Mn³⁺/Mn⁴⁺ ion-doped CDs. These fluorescence characteristics may be related to the functional groups on the surface of Mn³⁺/Mn⁴⁺ ion-doped CDs, and various functional groups can introduce different defects into the surface of Mn³⁺/Mn⁴⁺ ion-doped CDs as excitation energy traps, resulting in different fluorescence properties (Zhao et al., 2018). In addition, photostability is the premise of Mn³⁺/Mn⁴⁺ ion-doped CDs for biomolecular or metal ion detection and imaging. We studied the changes in fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs under different salt solution concentrations, time, and pH values. As shown in Supplementary Figures S3, S4, the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs remain almost unchanged with the increase in salt solution concentration and time. Based on this, we chose to carry out the related experiments of DA detection in the later stage without sodium chloride solution. As shown in Supplementary Figure S5, the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs is relatively low under strong acid or alkali conditions. Therefore, we choose pH 7 as the best condition for the anti-interference experiment of DA and related metal ions or biomolecules. Under the optimum conditions, the highest quantum yield of Mn³⁺/Mn⁴⁺ ion-doped CDs is 4.29%.

FIGURE 1

FIGURE 1. The morphology and optical properties of the samples were characterized (A) TEM and HRTEM (inset) images of Mn³⁺/Mn⁴⁺ ion-doped CDs (B) TEM particle size distribution. (C) AFM image of Mn³⁺/Mn⁴⁺ ion-doped CDs (D) XRD image of Mn³⁺/Mn⁴⁺ ion-doped CDs. (E) UV-vis absorption spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs. (F) The fluorescence emission spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs at different excitation wavelengths of 340–440 nm.

FT-IR and XPS evaluated the chemical composition and functional groups of Mn³⁺/Mn⁴⁺ ion-doped CDs. FT-IR spectra were shown in Figure 2A, the peak of Mn³⁺/Mn⁴⁺ ion-doped CDs were 3,367.8, 1,680.9, 1,600, 1,400, and 610 cm⁻¹, respectively, which were attributed to -NH₂/-OH, C=O, benzene ring, and Mn-O functional groups (Zhu et al., 2020). These results indicated abundant functional groups on the surface of Mn³⁺/Mn⁴⁺ ion-doped CDs, which makes Mn³⁺/Mn⁴⁺ ion-doped CDs have better dispersibility in an aqueous solution. In addition, XPS also further confirmed the presence of C=O, C=C, -NH₂/-OH and Mn-O chemical structures and elemental compositions on the surface of Mn³⁺/Mn⁴⁺ ion-doped CDs. The total spectrum of XPS shown in Figure 2B was mainly composed of C 1s, N 1s, O 1s, and Mn 2p, with the proportion of elements being 69.96, 8.55, 19.13, and 2.37% (Supplementary Table S1), respectively. As shown in Figure 2C, the high-resolution spectrum of C 1s showed three prominent peaks, and the binding energies of 284.7, 286, and 288 eV correspond to C=C, C-N, and C=O functional groups, respectively. The high-resolution spectrum of O 1s (Figure 2D) could be decomposed into two binding energies of 531.3 and 532.1 eV, which could be attributed to Mn-O and C-O, respectively. There were two binding energies of 399.1 and 400 eV in the high-resolution spectrum of N 1s, which could be attributed to C-N and N-H functional groups (Figure 2E). Moreover, Figure 2F shows the XPS spectrum of Mn 2p in the Mn³⁺/Mn⁴⁺ ion-doped CDs with four prominent peaks at 640.9, 645.8 eV, and 642.5, 653.3 eV, respectively, indicating that Mn³⁺ and Mn⁴⁺ ions exist in the surface of Mn³⁺/Mn⁴⁺ ion-doped CDs, and the ratio of Mn³⁺/Mn⁴⁺ on the surface was 0.82 (Wang J et al., 2019). FT-IR and XPS spectra showed a large number of hydrophilic functional groups on the surface of Mn³⁺/Mn⁴⁺ ion-doped CDs, which not only provided interaction sites for specific ions or biomolecules; but also improved the biocompatibility of the material itself.

FIGURE 2

FIGURE 2. Structural characterization of Mn³⁺/Mn⁴⁺ ion-doped CDs (A) FT-IR spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs. (B) the total XPS spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs and high-resolution C 1s (C), N 1s (D), O 1s (E), and Mn 2p (F) spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs.

Fluorescence detection of DA

Due to Mn³⁺ and Mn⁴⁺ions on the surface of CDs, the material had Fenton-like catalytic properties. The effect of DA on the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs at 390 and 478 nm was studied under optimal conditions. As shown in Figure 3A, with the gradual increase of DA content, the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs at 478 nm showed a continuous decrease, and the fluorescence intensity at 390 nm showed a continuously increasing trend. Figures 3B,C illustrate the linear relationship between ΔF/F₀ (ΔF = F-F₀, where F₀ and F are the fluorescence intensities of Mn³⁺/Mn⁴⁺ ion-doped CDs in the absence and presence of DA, respectively) and DA content. The linear range of Figure 3C was 100–275 nM with a correlation coefficient of 0.9966, and the detection limit was as low as 3 nM (n = 3); the linear range of Figure 3D was 325–525 nM with a correlation coefficient of 0.9918, and the detection limit was as low as 12 nM (n = 3). This value is equivalent to or even better than the previous fluorescence detection of DA (Table 1), indicating that this platform is suitable for the determination of DA.

FIGURE 3

FIGURE 3. Mn³⁺/Mn⁴⁺ ion-doped CDs for DA detection (A) Effect of DA content on the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs. (B) The curve relationship between fluorescence intensity at 390 nm and DA (0–550 nM) content (inset: the linear relationship between ΔF/F₀ and DA content (100–275 nM)) (C) The curve relationship between fluorescence intensity at 478 nm and DA (0–550 nM) content (inset: the linear relationship between ΔF/F₀ and DA (325–525 nM) content (D) Schematic illustration of the mechanism of Mn³⁺/Mn⁴⁺ ion-doped CDs for DA detection. (E) The fluorescence decay curves of Mn³⁺/Mn⁴⁺ ion-doped CDs in an aqueous solution without and with DA. (F) Anti-interference of fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs at 478 nm.

TABLE 1

TABLE 1. The comparison of this method with other DA detection in literature.

To study the mechanism of Mn³⁺/Mn⁴⁺ ion-doped CDs as a fluorescent probe for detecting DA, the valence states of Mn ion in Mn³⁺/Mn⁴⁺ ion-doped CDs without and containing DA were analyzed, respectively. As shown in Figure 2F and Supplementary Figure S6, compared with Mn³⁺/Mn⁴⁺ ion-doped CDs without DA, the Mn³⁺/Mn⁴⁺ ratio of DA-containing Mn³⁺/Mn⁴⁺ ion-doped CDs increased from 0.82 to 1.04. These results suggest that the mechanism of Mn³⁺/Mn⁴⁺ ion-doped CDs and DA was a redox property. As shown in Figure 3D, when DA was added to the aqueous solution containing Mn³⁺/Mn⁴⁺ ion-doped CDs, the DA reacted with Mn⁴⁺ ion on the surface of CDs, resulting in the conversion of phenolic hydroxyl functional groups on DA into O-quinone structure (Shi et al., 2020). At the same time, Mn⁴⁺ ions on the surface of CDs act as oxidants to obtain electrons, which changes the Mn³⁺/Mn⁴⁺ ratio on the surface of CDs, resulting in an increase or decrease in the fluorescence intensity of emission peak at 390 and 478 nm of Mn³⁺/Mn⁴⁺ ion-doped CDs with the rise of DA content. In addition, we also carried out the time-dependent single-photon counting spectra of Mn³⁺/Mn⁴⁺ ion-doped CDs under different conditions. As shown in Figure 3E, the average fluorescence lifetime of single Mn³⁺/Mn⁴⁺ ion-doped CDs was 4.82 ns. The average fluorescence lifetime of Mn³⁺/Mn⁴⁺ ion-doped CDs decreased to 4.62 ns after adding DA. The decreased average fluorescence lifetime indicates that the electron transfer between Mn³⁺/Mn⁴⁺ ion-doped CDs and DA was a dynamic fluorescence quenching induced process. Between them, Mn³⁺/Mn⁴⁺ ion-doped CDs and DA were used as energy receptors and energy donors, respectively. To induce Fenton-like catalysis between catechol functional groups on DA molecule and Mn³⁺/Mn⁴⁺ ion pairs on CDs surface, jointly constructing donor/receptor pairs of FRET.

Mn³⁺/Mn⁴⁺ ion pairs with Fenton-like catalytic properties exist on the surface of CDs, which have the potential to be used as fluorescent probes for oxidation/reduction of biomolecules or metal ions. DA, a suitable electron donor, will quench and enhance the fluorescence intensity at 478 and 390 nm respectively after mixing with Mn³⁺/Mn⁴⁺ ion-doped CDs. Firstly, we studied the fluorescence intensity changes of Mn³⁺/Mn⁴⁺ ion-doped CDs in NaCl solutions with different concentrations. It can be seen that the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs was not affected by the NaCl solution. Secondly, the effects of biomolecules or metal ions on the fluorescence intensity of Mn³⁺/Mn⁴⁺ ion-doped CDs were also investigated. As shown in Figure 3F and Supplementary Figure S7, biomolecules or metal ions such as Lys, Gly, Bi³⁺, Hf⁴⁺, In³⁺, Mn²⁺, and Na⁺ had almost no influence on the fluorescence intensity ratio F/F₀ of Mn³⁺/Mn⁴⁺ ion-doped CDs. SA had a weak fluorescence quenching effect on the fluorescence intensity ratio F/F₀ of Mn³⁺/Mn⁴⁺ ion-doped CDs, indicating that Mn³⁺/Mn⁴⁺ ion-doped CDs had relatively good selectivity for the above biomolecules or metal ions. Finally, to verify the Fenton-like catalytic properties of Mn³⁺/Mn⁴⁺ ion-doped CDs, we further studied the effect of oxidation/reduction biomolecules or metal ions on the fluorescence intensity ratio F/F₀ of Mn³⁺/Mn⁴⁺ ion-doped CDs. As shown in Figure 3F and Supplementary Figure S7, after adding oxidation/reduction biomolecules or metal ions such as AA, GSH, Cu²⁺, and Fe³⁺, the fluorescence intensity ratio F/F₀ of Mn³⁺/Mn⁴⁺ ion-doped CDs at 478 nm was relatively lower than that of the blank control group. At the same time, the fluorescence intensity ratio F/F₀ of Mn³⁺/Mn⁴⁺ ion-doped CDs at 390 nm was higher than that of the blank control group. The results indicate that Mn³⁺/Mn⁴⁺ ion-doped CDs have the potential as fluorescence probes for the detection of oxidation/reduction biomolecules or metal ions. However, the interference of other oxidation/reduction biomolecules or metal ions should be avoided during the detection of a certain oxidation/reduction biomolecule or metal ions, to enhance the accuracy and reliability of Mn³⁺/Mn⁴⁺ ion-doped CDs.

Conclusion

In conclusion, we reported a simple hydrothermal method for the synthesis of Mn³⁺/Mn⁴⁺ ion-doped blue-green fluorescent CDs. These CDs showed excellent photostability and Fenton-like catalytic properties; and could be used as biosensors for DA detection with detection limits of 3 and 12 nM, respectively. In addition, due to the Fenton-like catalytic properties of CDs, Mn³⁺/Mn⁴⁺ ion-doped CDs have a certain fluorescence response to oxidation/reduction biomolecules or metal ions. Therefore, during the detection of oxidation/reduction biomolecules or metal ions by Mn³⁺/Mn⁴⁺ ion-doped CDs, the interference of other oxidation/reduction substances should be avoided so as to enhance the accuracy of fluorescent probes. The present work not only provides a new method for the synthesis of materials with Fenton-like catalysis; but also demonstrates the great potential of these materials for the detection of oxidation/reduction biomolecules or metal ions.

Data availability statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.

Author contributions

All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.

Funding

This research was supported by the National Natural Science Foundation of China (No. 81972901), Distinguished Young Scholar Science Fund of the PLA (No. 2019-JCPY-ZD-120-40), Science Foundation of China University of Petroleum, Beijing (Nos. 2462019QNXZ02 and 2462019BJRC007). Science Foundation of China University of Petroleum (No. 2462020YXZZ018).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fbioe.2022.964814/full#supplementary-material

References

Ai, X. Z., Qiang, M., and Su, X. G. (2012). Nanosensor for dopamine and glutathione based on the quenching and recovery of the fluorescence of silica-coated quantum dots. Microchim. Acta 180 (3-4), 269–277. doi:10.1007/s00604-012-0925-z

CrossRef Full Text | Google Scholar

Anjali Devi, J. S., Salini, S., Aparna, R. S., and George, S. (2017). Boronic acid-functionalized nitrogen-doped carbon dots for fluorescent turn-on detection of dopamine. Microchim. Acta 184 (10), 4081–4090. doi:10.1007/s00604-017-2433-7

CrossRef Full Text | Google Scholar

Chen, Z. B., Zhou, T. H., and Ma, H. (2015). Gold nanoparticle-based colorimetric probe for dopamine detection based on the interaction between dopamine and melamine. Microchim. Acta 82 (5), 1003–1008. doi:10.1007/s00604-014-1417-0

CrossRef Full Text | Google Scholar

Du, R., Yao, L., Ding, F., Zou, P., Wang, Y. Y., Wang, X. X., et al. (2019). Colorimetric and fluorometric dual-signal determination of dopamine by the use of Cu-Mn-O microcrystals and C-dots. Sensors Actuators B Chem. 290, 125–132. doi:10.1016/j.snb.2019.03.107

CrossRef Full Text | Google Scholar

Gao, W. L., Wang, X., Liu, X. Q., Pang, X. J., Zhou, Y., Song, H, et al. (2018). Carbon dots with red emission for sensing of Pt²⁺, Au³⁺, and Pd²⁺ and their bioapplications in vitro and in vivo. ACS Appl. Mat. Interfaces 10, 1147–1154. doi:10.1021/acsami.7b16991

PubMed Abstract | CrossRef Full Text | Google Scholar

He, W. Z., Zhou, P., Liu, Q. Y., and Cui, T. H. (2020). Flexible micro-sensors with self-assembled graphene on a polyolefin substrate for dopamine detection. Biosens. Bioelectron. X. 167, 112473. doi:10.1016/j.bios.2020.112473

PubMed Abstract | CrossRef Full Text | Google Scholar

He, Y. S., Cao, H. X., Yue, M. Z., Wang, L., and Liang, G. X. (2018). Highly sensitive and selective dual-emission ratiometric fluorescence detection of dopamine based on carbon dots-gold nanoclusters hybrid. Sensors Actuators B Chem. 265, 371–377. doi:10.1016/j.snb.2018.03.080

CrossRef Full Text | Google Scholar

Jin, H., and Wang, Z. G. (2018). Military brain science-how to influence future wars. Chin. J. Traumatology 21 (5), 277–280. doi:10.1016/j.cjtee.2018.01.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, S., Shi, F., Zhao, X., Chen, L., and Su, X. (2013). 3-Aminophenyl boronic acid-functionalized CuInS2 quantum dots as a near-infrared fluorescence probe for the determination of dopamine. Biosens. Bioelectron. X. 47, 379–384. doi:10.1016/j.bios.2013.03.055

PubMed Abstract | CrossRef Full Text | Google Scholar

Mao, Y., Bao, Y., Han, D., Li, F., and Niu, L. (2012). Efficient one-pot synthesis of molecularly imprinted silica nanospheres embedded carbon dots for fluorescent dopamine optosensing. Biosens. Bioelectron. X. 38 (1), 55–60. doi:10.1016/j.bios.2012.04.043

PubMed Abstract | CrossRef Full Text | Google Scholar

Natan, M., Banin, E., Gedanken, A., and Luong, J. H. T. (2019). Antibacterial activity against methicillin-resistant staphylococcus aureus of colloidal polydopamine prepared by carbon dot stimulated polymerization of dopamine. Nanomaterials 9 (12), 1731. doi:10.3390/nano9121731

CrossRef Full Text | Google Scholar

Ngo, K. T., Michael, A. C., and Weber, S. G. (2017). Monitoring dopamine responses to potassium ion and nomifensine by in vivo microdialysis with online liquid chromatography at one-minute resolution. ACS Chem. Neurosci. 8 (2), 329–338. doi:10.1021/acschemneuro.6b00383

PubMed Abstract | CrossRef Full Text | Google Scholar

Poo, M. M., and Tan, T. N. (2016). Brain science and brain-inspired intelligence technology an overview. BCAS 31 (7), 725–736. doi:10.12691/jbms-8-1-1

CrossRef Full Text | Google Scholar

Tammina, S. K., Yang, D., Koppala, S., Cheng, C., and Yang, Y. (2019). Highly photoluminescent N, P doped carbon quantum dots as a fluorescent sensor for the detection of dopamine and temperature. J. Photochem. Photobiol. B Biol. 194, 61–70. doi:10.1016/j.jphotobiol.2019.01.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Tang, Z. D., Jiang, K., Sun, S., Wang, Y, H., and Lin, H. W. (2018). A conjugated carbon-dot-tyrosinase bioprobe for highly selective and sensitive detection of dopamine. Analyst 144 (2), 468–473. doi:10.1039/c8an01659c

PubMed Abstract | CrossRef Full Text | Google Scholar

Tian, T., Ge, Y. L., and Song, G. W. (2017). One-pot synthesis of boron and nitrogen co-doped carbon dots as the fluorescence probe for dopamine based on the redox reaction between Cr(VI) and dopamine. Sensors Actuators B Chem. 240, 1265–1271. doi:10.1016/j.snb.2016.09.114

CrossRef Full Text | Google Scholar

Tu, N. N., and Wang, L. Y. (2013). Surface plasmon resonance enhanced upconversion luminescence in aqueous media for TNT selective detection. Chem. Commun. 49 (56), 6319–6321. doi:10.1039/c3cc43146k

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, C. J., Yang, M., Yao, Z. R., Xue, W. M., Fan, J., Liu, E., et al. (2021). Biocompatible sulfur nitrogen co-doped carbon quantum dots for highly sensitive and selective detection of dopamine. Colloids Surfaces B Biointerfaces 205, 111874. doi:10.1016/j.colsurfb.2021.111874

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, J., Liu, W., Zhao, Q. B., Yao, L., Ding, F., Zou, P., et al. (2019). Colorimetric and fluorometric dual-signal determination of dopamine by the use of Cu-Mn-O microcrystals and C-dots. Sensors Actuators B Chem. 290, 125–132. doi:10.1016/j.snb.2019.03.107

CrossRef Full Text | Google Scholar

Wang, J. G., Chen, Q. Q., Li, H. F., Tang, B. Z., Zhou, L., Jiang, X., et al. (2019). An easily available ratiometric reaction-based AIE probe for carbon monoxide light-up imaging. Anal. Chem. 91 (15), 9388–9392. doi:10.1021/acs.analchem.9b02691

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, J. H., Zhang, H. L., Wang, H. Q., Lin, S., Zhao, Y. D., Luo, Q. M., et al. (2010). Bioconjugation of concanavalin and CdTe quantum dots and the detection of glucose. Colloids Surfaces A Physicochem. Eng. Aspects 364 (1-3), 82–86. doi:10.1016/j.colsurfa.2010.04.041

CrossRef Full Text | Google Scholar

Yang, J. J., Hao, P. F., Qiu, F. D., Liu, M. X., Zhang, Q., Shi, D. X., et al. (2015). Synthesis, optical properties of multi donor-acceptor substituted AIE pyridine derivatives dyes and application for Au³⁺ detection in aqueous solution. Dyes Pigm. 116, 97–105. doi:10.1016/j.dyepig.2015.01.005

CrossRef Full Text | Google Scholar

Zhao, C. X., Hua, J. H., Yang, J., and Yang, Y. L. (2018). Hydrothermal synthesis of nitrogen-doped carbon quantum dots as fluorescent probes for the detection of dopamine. J. Fluoresc. 28 (1), 269–276. doi:10.1007/s10895-017-2189-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, J., Zhao, L., Lan, C., and Zhao, S. (2016). Graphene quantum dots as effective probes for label-free fluorescence detection of dopamine. Sensors Actuators B Chem. 223, 246–251. doi:10.1016/j.snb.2015.09.105

CrossRef Full Text | Google Scholar

Zhao, L., Bai, Y. F., Feng, F., and Yang, X. M. (2021). Exploration of carbon dots derived from epimedium towards detecting dopamine and hydrogen peroxide. Colloids Surfaces A Physicochem. Eng. Aspects 627, 127179. doi:10.1016/j.colsurfa.2021.127179

CrossRef Full Text | Google Scholar

Zhu, P. D., Lin, K. P., Lin, C., Shi, J. H., Li, S., Yu, S., et al. (2020). Dual-response detection of oxidized glutathione, ascorbic acid, and cell imaging based on pH/redox dual-sensitive fluorescent carbon dots. ACS Omega 5 (9), 4482–4489. doi:10.1021/acsomega.9b03730

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhu, P. D., Zhao, X. L., Chen, X. Y., Li, S. Z., Xu, Q., Li, J., et al. (2021). Yellow emission N-doped fluorescent carbon dots as fluorescent nanoprobes for the detection of L-threonine in real samples. New J. Chem. 45 (17), 10798–10801. doi:10.1039/D1NJ01812D

CrossRef Full Text | Google Scholar

Zhuo, S. J., Li, H., Fang, J., Zhang, P., Du, J. Y., Zhu, C. Q., et al. (2019a). Facile fabrication of fluorescent Fe-doped carbon quantum dots for dopamine sensing and bioimaging application. Analyst 144 (2), 656–662. doi:10.1039/c8an01741g

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhuo, S. J., Li, M., Wang, J., Zhu, C. Q., and Du, J. (2019b). Manganese(II)-doped carbon dots as effective oxidase mimics for sensitive colorimetric determination of ascorbic acid. Microchim. Acta 186 (12), 745. doi:10.1007/s00604-019-3887-6

PubMed Abstract | CrossRef Full Text | Google Scholar