Efficacy of Probiotics for Irritable Bowel Syndrome: A Systematic Review and Network Meta-Analysis

Introduction

Irritable bowel syndrome (IBS) is a common and chronic gastrointestinal (GI) condition characterized by abdominal pain, bloating, and changes in bowel habits associated with altered stool form, which can affect the quality of life and work productivity of patients (Mearin et al., 2016; Camilleri, 2021). In terms of clinical epidemiology, the prevalence of IBS varies substantially among different countries and different diagnostic criteria, ranging from 1.1% to 45% (Black and Ford, 2020); Furthermore, there is a higher prevalence of IBS in women than in men (12% vs. 8.6%) (Oka et al., 2020). IBS can be diagnosed by reviewing the clinical findings based on the Rome Criteria rather than basing the diagnosis on definite biological markers and organic lesions in patients with IBS (Lacy and Patel, 2017).

There are trillions of microbes residing in the human GI tract, which is over 150 times the number of genes in the human genome (Qin et al., 2010; Raskov et al., 2016). Beneficial commensal bacteria, which play an important role in healthy individuals, can contribute to the upregulation of anti-inflammatory genes and downregulation of pro-inflammatory genes (Plaza-Diaz et al., 2014). In IBS cases, the reduction of microbiome diversity, gut barrier deficiency, gut-brain signaling disorders, and immune disorders are significantly related to the abnormal function of the GI tract (Raskov et al., 2016). Liu et al. (2016) found that when compared with healthy controls, the diarrhea predominant IBS (IBS-D) group had lower biodiversity of microbial communities, which were dominated by Bacteroides and Prevotella genera. Moreover, a decrease in probiotic species and an increase in pathogenic species were also found to be common in IBS cases (Ringel and Ringel-Kulka, 2015).

Probiotics, available in various dietary components or by prescription, contain live microorganisms in which most bacteria are similar to the beneficial bacteria that are naturally present in the human GI tract (Wilkins and Sequoia, 2017). Lactobacillus and Bifidobacteria are often used in probiotic products and have been studied in clinical trials (Kligler and Cohrssen, 2008; Raskov et al., 2016). The efficacy and safety of probiotic products for the treatment of IBS are supported by an increasing number of clinical studies. A meta-analysis (Ford et al., 2018) with 53 randomized controlled trials (RCTs) involving 5,545 patients provided data regarding the potential efficacy of probiotic combinations and specific probiotic species or strains for improving global IBS symptoms and abdominal pain. In addition to relieving symptoms, probiotics have been demonstrated to improve the quality of life (QOL) and diversify the microbial community of IBS cases in several studies (Sun et al., 2018; Preston et al., 2018).

To the best of our knowledge, although the efficacy and safety of probiotics have been confirmed by numerous studies, the best species for probiotics used in the treatment of IBS have not been identified yet (Gwee et al., 2019). Therefore, in the present study, we performed a systematic review and network meta-analysis (NMA) to compare the efficacy of probiotics for IBS to identify the best interventions.

Methods

A systematic review and NMA were carried out in accordance with the Preferred Reporting Items for Systematic Review and Meta-analysis extension statement, including NMA (PRISMA-NMA) (Page et al., 2021).

Search Strategy

The databases, including PubMed, Cochrane Library, Web of Science, and Medline, were searched systematically by two independent researchers on August 25, 2021, to identify RCTs exploring the efficacy of probiotics for patients with IBS. The search terms in PubMed were as follows: (irritable bowel syndrome) OR (IBS) AND (probiotics) OR (probiotic) OR (Saccharomyces) OR (Escherichia) OR (Bifidobacterium) OR (Bacillus) OR (Lactobacillus) OR (Clostridium) AND ([randomized controlled trial{Publication Type}] OR [clinical trial{Publication Type}]). In addition, the lists of references from the previous systematic review and meta-analysis in this field were also reviewed to identify any missing literature.

Eligible Criteria

Studies that met the following criteria were eligible for NMA.

1. RCTs that compared the efficacy and tolerability of probiotic with placebo or another probiotic for patients with IBS were eligible.

2. The patients included in all RCTs had a well-established diagnosis of IBS, and there were no limitations on age, sex, countries, types of IBS, and the publication year of the RCTs.

3. The probiotics included the following species: Saccharomyces boulardii (S. boulardii), Saccharomyces cerevisiae (S. cerevisiae), Escherichia coli (E. coli), Bifidobacterium bifidum (B. bifidum), Bacillus coagulans (B. coagulans), Lactobacillus acidophilus (L. acidophilus), Lactobacillus GG (LGG), Lactobacillus paracasei (L. paracasei), Lactobacillus salivarius (L. salivarius), Lactobacillus plantarum (L. plantarum), Bifidobacterium longum (B. longum), Lactobacillus casei (L. casei), Lactobacillus gasseri (L. gasseri), Bifidobacterium infantis (B. infantis), Clostridium butyricum (C. butyricum), Lactobacillus reuteri (L. reuteri), and Bifidobacterium lactis (B. lactis), etc.;

4. The dosages of the probiotics and the duration of each intervention were recorded in detail.

5. The patients were required to be followed up for at least 1 week, and the studies had to report the outcome of symptom relief rate, assessment of global and individual symptom scores, QOL, and adverse events.

Exclusion Criteria

The exclusion criteria included the following.

1. Duplicated studies and studies that were not related to our research topic were excluded.

2. Non-RCTs, observational studies, single-arm studies, case reports, reviews, meta-analyses, letters, protocols, and other such sources were excluded.

3. Papers published in a language other than English were excluded.

4. Papers without full text (or in which only the abstract was available) or the data of our target outcomes were excluded.

5. Participants with other comorbidities, such as inflammatory bowel disease, celiac disease, lactose intolerance, were excluded from the study.

Data Extraction and Risk of Bias

Two authors independently extracted the following information from each study: author, year of publication, country, sample size, age of patients, subtypes of IBS, comparison, and treatment details (types and dosages of probiotics, response rate of placebo, duration of treatment, and outcome measures).

Two authors evaluated the risk of bias for each included RCT with the help of measures displayed in the Cochrane Handbook for Systematic Reviewers (version 5.1.0), which includes seven indicators: 1) random sequence generation (selection bias), 2) allocation concealment (selection bias), 3) blinding of patients and personnel (performance bias), 4) blinding of outcome assessment (detection bias), 5) incomplete outcome data (attrition bias), 6) selective reporting (reporting bias), and 7) other bias. Each indicator contained three levels: low risk, unclear risk, or high risk of bias.

If there were any inconsistencies or disagreements in the process of data extraction and quality assessment, the two authors discussed these issues or an independent expert in this field was consulted to reach a consensus.

Statistical Analysis

NMA was performed using the Stata software version 15.0. For categorical data, we estimated the summary odds ratio (OR) with a 95% confidence interval (95% CI), and for continuous data, we estimated the summary standardized mean difference (SMD) with 95% CI. We showed the direct comparison between different interventions using a network diagram, in which the size of the nodes represents the sample size of each intervention, and the thickness of the continuous lines connecting the nodes indicates the number of studies directly comparing the two interventions. Subsequently, global inconsistency was evaluated, and the local inconsistency assessment was performed using the node-splitting method to check whether the estimated effects from the direct comparisons were consistent with those from the indirect comparisons. P>0.05 indicates that there were no significant differences of estimated effects between direct and indirect comparisons, thus the consistency model was used; otherwise, the inconsistency model was used. We assessed network heterogeneity across all treatment contrasts using I² statistics, and loop-specific heterogeneity using the τ² statistics. To rank the efficacy and safety of the interventions, we calculated the probabilities of the surface under the cumulative ranking curve (SUCRA) between all interventions for the primary and secondary outcomes. League tables containing both direct and indirect comparisons were also performed to summarize the outcomes of each indicator. Additionally, we also conducted a meta-regression analysis to explore whether the lengths and doses of interventions were associated with efficacy and adverse events of probiotics in IBS, if so, a subgroup analysis was performed.

Results

Study Selection and Characteristics

As is shown in Figure 1, we identified a total of 676 articles in our initial search of databases and review of the lists of references. A total of 253 papers were included after accounting for the presence of duplicate papers. Furthermore, we reviewed the titles and abstracts of these papers carefully, and 162 of them were excluded because they were not relevant to our research topic. The full texts of the remaining 91 papers were further analyzed, and 48 articles were excluded (the detailed reasons for exclusion are shown in Figure 1). Ultimately, 43 RCTs were included in the present study.

FIGURE 1

Figure 1 Flow diagram of assessment of studies.

Among the included studies, 29 RCTs (Niedzielin et al., 2001; Bauserman and Michail, 2005; Niv et al., 2005; O’Mahony et al., 2005; Whorwell et al., 2006; Sinn et al., 2008; Enck et al., 2009; Ligaarden et al., 2010; Choi et al., 2011; Guglielmetti et al., 2011; Kabir et al., 2011; Dapoigny et al., 2012; Ducrotté et al., 2012; Kruis et al., 2012; Abbas et al., 2014; Lyra et al., 2016; Majeed et al., 2016; Pineton et al., 2015; Spiller et al., 2016; Stevenson et al., 2014; Thijssen et al., 2016; Pinto-Sanchez et al., 2017; Sun et al., 2018; Sudha et al., 2018; Madempudi et al., 2019; Andresen et al., 2020; Gayathri et al., 2020; Martoni et al., 2020; Gupta and Maity, 2021) were related to 15 probiotic species, including the following species: L. plantarum (4 RCTs), L. acidophilus (4 RCTs), B. coagulans (4 RCTs), S. boulardii (3 RCTs), S. cerevisiae (3 RCTs), and L. casei (2 RCTs). One RCT (Sadrin et al., 2020), related to two different strains of L. acidophilus, was also identified as a RCT exploring the efficacy of a probiotic specie (L. acidophilus) on IBS. Unfortunately, there was only one article with respect to B. lactis, L. GG, L. salivarius, B. longum, C. butyricum, and L. reuteri.

13 RCTs (Kim et al., 2003; Kajander et al., 2005; Kim et al., 2005; Guyonnet et al., 2007; Kajander et al., 2008; Drouault-Holowacz et al., 2008; Zeng et al., 2008; Agrawal et al., 2009; Søndergaard et al., 2011; Begtrup et al., 2013; Roberts et al., 2013; Jafari et al., 2014; Wong et al., 2015) were associated with 5 types of probiotic combinations that frequently used in clinical trials as follows: 1) 1 strains of Bifidobacterium, 1 strains of Lactobacillus, and 1 strains of Streptococcus (hereinafter referred to as 1B1L1S); 2) 1 strains of Bifidobacterium and 2 strains of Lactobacillus (1B2L); 3) 1 strains of Bifidobacterium, 2 strains of Lactobacillus, and 1 strains of Propionibacterium (1B2L1P); 4) 1 strains of Bifidobacterium, 2 strains of Lactobacillus, and 1 strains of Streptococcus (1B2L1S); 5) 3 strains of Bifidobacterium, 4 strains of Lactobacillus, and 1 strains of Streptococcus (3B4L1S). The study sample size ranged from 25 to 443, and a total of 5,531 participants were included in the NMA. The detailed patient characteristics are shown in Table 1.

TABLE 1

Table 1 Characteristics of RCTs about the efficacy of probiotics in irritable bowel syndrome.

Risk of Bias

The risk of bias assessment for all the included RCTs is presented in Supplementary Figure 1 (Figure S1). Overall, twelve trials were judged to have a low risk of bias across all domains. Two trials were judged to have a high risk of bias for blinding of the outcome assessment. Almost all the RCTs were judged to have a low risk of bias for allocation concealment, blinding of participants and personnel, incomplete outcome data, and selective reporting, except for two trials that had unclear risk in the domain of allocation concealment, one trial that had an unclear risk for blinding of participants and personnel, and one trial that had an unclear risk for incomplete outcome data.

Primary Outcomes: Effect of Different Probiotic Species on Symptom Relief Rate

A total of 19 RCTs explored the efficacy of probiotics on symptom relief rate, and the network plots are presented in Figure 2A. The results of the global and local inconsistency tests are presented in Figure S2 and Table S1. Both tests showed that there was no significant inconsistency between the direct comparisons and indirect comparisons; thus, the consistency model was used. The NMA revealed that B. coagulans (OR 60.73, 95% CI, 14.83 to 248.61), L. plantarum (OR 15.62, 95% CI 2.90 to 84.21), and L. acidophilus (OR 3.00, 95% CI 1.03 to 8.68) had a greater effect on symptom relief rate in patients with IBS compared with placebo (PLA). The SUCRA analysis (Table S2 and Figure S3) and league table (Table 2) showed that B. coagulans had the best rank among all the treatment interventions; meanwhile, L. plantarum ranked second, L. acidophilus ranked third, and PLA ranked last.

FIGURE 2

Figure 2 The network plots. (A) was the network plot about the effect of probiotics on improving the symptom relief rate of IBS patients; (B) Global symptom score; (C) Abdominal pain score; (D) Bloating score; (E) Straining score; (F) QOL score; (G) Adverse events; (H) Subgroup analysis on treatment length of probiotics for improving the abdominal pain of IBS patients; (I) Subgroup analysis on treatment length of probiotics for improving the straining scores of IBS patients.

TABLE 2

Table 2 Odds ratio (OR) with 95% confidence interval on symptom relief rate.

Significant heterogeneity was observed across all treatment contrasts (I² = 85.5%), but no evidence of loop-specific heterogeneity was found (τ^{2 =} 0). The meta-regression analysis by treatment dose and length did not significantly influence the SMD estimates for this outcome (Figures 3A, B).

FIGURE 3

Figure 3 Meta-regression by treatment lengths and doses for all primary and secondary outcomes: (A, C, E, G, I, K, M) indicate the meta-regression analysis by treatment lengths for the outcomes of symptom relief rate, global symptom score, abdominal pain score, bloating score, straining scores, QOL, and adverse events, respectively; (B, D, F, H, J, L, N) indicate the meta-regression analysis by doses for the outcomes of symptom relief rate, global symptom score, abdominal pain score, bloating score, straining scores, QOL, and adverse events, respectively. The abscissa (X) represents the duration (weeks) or dose (×108), and the ordinate (Y) represents the SMD.

Primary Outcomes: Effect of Different Probiotic Species on Global Symptom Scores

A total of 13 RCTs comparing the effect of probiotics on the global symptom scores of patients with IBS were included. The network plot is shown in Figure 2B. Both the global and local inconsistency tests revealed a significant inconsistency between direct and indirect comparisons (Figure S4 and Table S3), which indicated that the inconsistency model should be used. The result of NMA revealed a significant improvement in the global symptom scores in patients who received B. coagulans (SMD −1.99, 95% CI −2.39 to −1.59) and Bifidobacterium infantis (SMD −0.74, 95% CI −1.47 to −0.01) compared with those who received PLA. Based on the SUCRA analysis (Table S4 and Figure S5) and league table (Table 3), B. coagulans, C. butyricum, and Bifidobacterium longum ranked as the top three interventions in improving the global symptom scores of patients with IBS, while L. plantarum ranked last.

TABLE 3

Table 3 Standardized mean differences (SMDs) and 95% CI on global symptom scores.

In this outcome, we found obvious heterogeneity across all treatment contrasts (I² = 91.2%), but no heterogeneity (τ^{2 =} 0) in the loop of NMA. Meta-regression analysis showed that treatment dose and length did not significantly influence the SMD estimates for global symptom scores (Figures 3C, D).

Primary Outcomes: Effect of Different Probiotic Species on Abdominal Pain Scores

A total of 16 RCTs reported the effect of probiotics on abdominal pain scores in patients with IBS, and the network diagram is shown in Figure 2C. Both the global inconsistency test (Figure S6) and node-splitting assessment (Table S5) showed no significant inconsistency between direct and indirect comparisons; therefore, the consistency model was used. The results of NMA (Table 4) revealed a significant improvement in the abdominal pain scores in patients who received B. coagulans (SMD −1.71, 95% CI −2.15 to −1.27) and S. cerevisiae (SMD −0.54, 95% CI −1.08 and −0.00) than those who received PLA. The SUCRA analysis (Table S6 and Figure S7) demonstrated that B. coagulans ranked first in improving the abdominal pain scores of patients with IBS, while S. cerevisiae ranked second, C. butyricum ranked third, and S. boulardii ranked last.

TABLE 4

Table 4 Standardized mean differences (SMDs) and 95% CI on abdominal pain scores.

There was a significant heterogeneity across all treatment contrasts (I² = 90.4%), but no loop-specific heterogeneity (τ^{2 =} 0) in this outcome. The meta-regression analysis (Figures 3E, F) showed that treatment duration, as a confounder, can significantly influence the efficacy of probiotics in improving the symptoms of abdominal pain (Coef = −2.30; p= 0.035) in patients with IBS.

Subsequently, we performed a subgroup analysis of treatment duration (Figure 2D). No evidence of loop-specific heterogeneity was found (τ^{2 =} 0). The consistency model was used based on the results of the global inconsistency test (Figure S8) and node-splitting assessment (Table S7), both of which showed that there was no significant inconsistency between the direct and indirect comparisons. The results of the NMA indicated that the patients who received B. coagulans (8 w) (SMD −2.13, 95% CI −2.84 to −1.41), B. coagulans (11 w/13 w) (SMD −1.61, 95% CI −2.46 to −0.76), and S. cerevisiae (10 w) (SMD −1.00, 95% CI −2.00 to −0.00) had lower abdominal pain scores than those who received PLA. Based on the results of the SUCRA (Table S8 and Figure S9) and league table (Table S9), we found that B. coagulans (8 w), B. coagulans (11 w/13 w), and S. cerevisiae (10 w) ranked as the top three among all the interventions, while S. boulardii (4 w/6 w) ranked last.

Primary Outcomes: Effect of Different Probiotic Species on Bloating Scores

The effect of probiotics on abdominal bloating was reported in 13 RCTs, and the network plot is presented in Figure 2E. The consistency model was used as there was no significant inconsistency found in both the global and local inconsistency tests (Table S10 and Figure S10). Only patients who received B. coagulans (SMD −1.42, 95% CI −1.87 to −0.96) had a significant improvement in abdominal bloating scores compared with those who received PLA. The SUCRA analysis (Table S11 and Figure S11) and league table (Table S12) indicated that B. coagulans ranked best among all the other interventions, while B. infantis ranked second, L. acidophilus ranked third, and L. plantarum ranked last.

In this primary outcome, the heterogeneity across all treatment contrasts was significant (I² = 90.0%), which was in contrast to the loop-specific heterogeneity (τ^{2 =} 0). Additionally, the meta-regression analysis using treatment length and dose did not influence the SMD estimates for bloating sores significantly (Figures 3G, H).

Primary Outcomes: Effect of Different Probiotic Species on Straining Scores

There were only seven RCTs involved when comparing the effect of probiotics on straining scores. The network plot is shown in Figure 2F. Due to a lack of inconsistency resources, the consistency model was used. The results of the NMA showed that the patients who were administered B. coagulans (SMD −1.29, 95% CI −1.63 to −0.94) had lower straining scores than those who were administered PLA. Based on the SUCRA analysis in Table S12 and Figure S13 and the league table in Table S14, B. coagulans had the best rank among all other interventions in improving straining scores of patients with IBS, followed by B. infantis and L. plantarum; meanwhile, PLA ranked last.

The heterogeneity was significant across all treatment contrasts (I² = 88.7%) and the meta-regression analysis (Figures 3I, J) showed that treatment duration can significantly influence the efficacy of probiotics in improving the symptoms of straining (Coef = −3.15; p = 0.020) in patients with IBS. Therefore, we performed a subgroup analysis of treatment lengths (Figure 2G). Both the global and local inconsistency tests showed a significant inconsistency (Table S13 and Figure S15), which indicated that the inconsistency model should be used. The NMA results revealed a significant improvement in symptoms of straining in the patients who received B. coagulans (8 w) (SMD −1.60, 95% CI −2.12 to −1.08) compared to those who received PLA. Additionally, the SUCRA (Table S16 and Figure S14) and league table (Table S17) showed that B. coagulans (8 w) ranked first, S. boulardii (4 w) ranked second, B. coagulans (4 w) ranked third, while S. boulardii (6 w) ranked last.

Secondary Outcomes: Effect of Different Probiotic Species on QOL

The effect of probiotics on the QOL of patients with IBS was only reported in four RCTs. The network plot is shown in Figure 2H. The consistency model was used due to the lack of inconsistent resources. The NMA results showed that there are no treatment interventions better than PLA in improving the QOL of patients with IBS. The results of the SUCRA analysis, available in Table S18, 19 and Figure S15, showed that L. plantarum ranked first in improving the QOL of patients with IBS; meanwhile, S. boulardii ranked second, L. acidophilus ranked third, and PLA ranked last. No significant heterogeneity was observed across all treatment contrasts (I² = 0.0%). Meta-regression by treatment length and dose did not significantly influence the SMD estimates for QOL (Figures 3K, L).

Secondary Outcomes: Adverse Events (AEs)

Total AEs were reported in 13 RCTs, and the network plot is presented in Figure 2I. The consistency model was used due to a lack of inconsistent resources. The NMA results revealed that only patients who received L. acidophilus had a lower incidence of AEs compared with patients who received PLA (OR 0.47, 95% CI 0.32, 0.67). Based on the SUCRA analysis (Table S20 and Figure S16) and league table (Table S21), L. acidophilus ranked first among all the other interventions, PLA ranked second, L. reuteri ranked third, and C. butyricum ranked last. Additionally, we observed significant heterogeneity (I² = 59.3%) across all studies in this outcome. Meta-regression by treatment duration and dose did not significantly influence the SMD estimates for the incidence of AEs (Figures 3M, N).

Comparisons of B. coagulans With Different Probotic Combinations for the Treatment of IBS

The evidences above revealed that B. coagulans was more effective in improving several IBS related symptoms than other probiotic species, thus, we further explored its efficacy compared to different types of probiotic combinations. Interestingly, based on the results from SUCRA analysis (Figures S17-20 and Tables S22-25), we found that B. coagulans had the best rank among all the probiotic combinations in improving symptom relief rate, as well as global symptom, abdominal pain, and bloating scores. Simultaneously, the probiotic combinations 1B2L1S (with 1 strains of Bifidobacterium, 2 strains of Lactobacillus, and 1 strains of Streptococcus) ranked second in improving global symptom and abdominal pain scores.

Comparisons of Different Strains of B. coagulans for the Treatment of IBS

As for the symptom relief rate and global symptom scores, only B. coagulans Unique IS2 was involved, thus it is not difficult to conclude that B. coagulans Unique IS2 ranked first in improving the symptom relief rate and global symptoms of IBS relatively among all interventions. In terms of the ability to alleviate abdominal pain of IBS patients, the league table (Table S26) showed that B.coagulans MTCC5856 ranked first and B.coagulans Unique IS2 ranked second, which was consistent with the result of abdominal bloating scores (Table S27). Lastly, B.coagulans Unique IS2 also exhibited the highest probability to be the optimal strains in improving the symptom of straining (Table S28).

Discussion

To date, the guidelines on the treatment of IBS with probiotics remain controversial. The British Society of Gastroenterology guidelines (Vasant et al., 2021) on the management of IBS, which was updated in 2021, reported that probiotics may be an effective treatment for improving global symptoms and abdominal pain in patients with IBS, which was consistent with the recommendations of the Canadian Association of Gastroenterology (Moayyedi et al., 2019) and the Japanese Society of Gastroenterology (Fukudo et al., 2021). In contrast, the guidelines from the American College of Gastroenterology (Lacy et al., 2021) suggest against the use of probiotics for the treatment of global IBS symptoms. Despite the controversies among different clinical practice guidelines, the effectiveness of probiotics in treating patients with IBS has not been completely validated before (Gwee et al., 2019) due to significant heterogeneity, publication bias, and inconsistent results in some meta-analyses, as well as several small sample size RCTs without rigorous endpoints based on US Food and Drug Administration (USFDA), and multiple types of probiotics without adequate validations, which may also contribute to the low level of evidence in the guidelines (Lacy et al., 2021).

To the best of our knowledge, this is the first study to simultaneously compare the efficacy of different probiotic species used for the treatment of IBS. The strength of this systematic review and NMA is that we performed a meta-regression analysis on the duration and doses of different treatments to explore whether these factors influence the outcomes, and if there were any influences, a subgroup analysis was conducted, adding rigor to our results. The main findings of our NMA were that B. coagulans was effective in increasing the symptom relief rate of patients with IBS, as well as in improving global symptoms, abdominal pain, bloating, and straining. Moreover, the meta-regression on treatment duration can significantly influence the SMD estimates of abdominal pain and straining scores, which indicates that increased treatment duration was a factor that negatively influenced both outcomes. The subgroup analysis of treatment durations indicated that the administration of B. coagulans for 8 weeks increased the effectiveness in the improvement of symptoms of abdominal pain and straining in patients with IBS. Unfortunately, due to insufficient original data of the included RCTs, NMA could not be performed to determine its efficacy in improving the QOL of patients with IBS and the AEs associated with this disease. Additionally, B. coagulans still had significant effects in improving symptom relief rate, as well as global symptom, abdominal pain, and bloating scores compared to different types of probiotic combinations in present study, which further validated the pronounced efficacy of B. coagulans.

B. coagulans is a spore-forming bacteria widely used in commercial probiotic formulations owing to its outstanding properties which are partly associated with its encapsulated coating that can protect it from drought conditions and allow it to survive and proliferate in various secretions of the GI tract, such as gastric acid, pepsin, pancreatin, digestive enzymes, and bile (Mu and Cong, 2019). Additionally, it can produce a range of proteins, antimicrobial substances, and vitamins, as well as modulate the gut microbiome, strengthen the body’s immunity (Elshaghabee et al., 2017; Maity et al., 2020), and treat various ailments such as Helicobacter pylori infection, gingivitis, and IBD. Although there are only a few RCTs regarding the use of different strains of B. coagulans for patients with IBS, their efficacy and safety are apparent. In two different studies, Madempudi et al. (Sudha et al., 2018; Madempudi et al., 2019) demonstrated that B. coagulans Unique IS2 was effective in relieving IBS-associated symptoms, such as abdominal pain, bloating, urgency, and straining, in improving stool consistency, and in increasing the serum anti-inflammatory factor IL-10 in children and adults with acceptable tolerability. Majeed et al. (2016) and Gupta et al. (Gupta and Maity, 2021) found that B. coagulans can improve the QOL of patients with IBS-D and significantly relieve the symptoms of diarrhea and constipation in the patients. To the best of our knowledge, our study is the first to compare the effectiveness of B. coagulans with other interventions and confirms the significant efficacy of B. coagulans in patients with IBS, especially at 8 weeks. Nevertheless, it is valuable to note that the benefits provided by probiotics are strain-specific rather than species-specific and genus-specific (Majeed et al., 2016); therefore, the health benefits may vary based on different strains of B. coagulans. Thus, we compared the efficacy of different strains of B. coagulans for the treatment of IBS, which revealed that B. coagulans Unique IS2 exhibited the highest probability to be the optimal strains in improving symptom relief rate, global symptom scores, and the symptom of straining. Meanwhile, B.coagulans MTCC5856 ranked first in alleviating abdominal pain and abdominal bloating.

Increasing evidence, including the biopsychosocial model of IBS, suggests that in patients with IBS, psychosocial factors (anxiety, stress) can be secondary to abdominal symptoms (bottom-up); in turn, intestinal (physiological) functions, such as visceral sensitivity, motility, and stress reactivity of the gut can be impacted by psychosocial factors (top-down) (Fond et al., 2014). It is believed that both the gut microbiome and the gut-brain axis play an important role in the bidirectional signaling between the brain and the gut (Schmidt, 2015) through the neurological, endocrine, and immune pathways (Carabotti et al., 2015), especially via the former two pathways (Ng et al., 2018). It has also been reported that the gut microbiome has a direct influence on stress reactivity by stimulating the vagus nerve and the enteric nervous system (Ng et al., 2018), as well as by synthesizing and modulating neurotransmitters (Yano et al., 2015). Thus, in IBS cases, the disturbed QOL attributed to comorbidity of abdominal symptoms, extra-intestinal symptoms, and psychiatric symptoms (Creed et al., 2001; Spiegel et al., 2004) can be improved by alleviating IBS-related pain (abdominal symptoms) (El-Serag and Olden, 2002) by regulating the gut microbiome with probiotic therapies.

Interestingly, a previous study found that some probiotics, such as Lactobacillus acidophilus NCFM, can modify the expression of pain-associated receptors, such as μ-opioid and cannabinoid receptors, in the GI tract in mice and humans (Rousseaux et al., 2007; Ringel-Kulka et al., 2014), thereby improving the symptoms of abdominal pain. Some bacterial species, such as Enterobacteriaceae and Clostridia, are more prone to producing intestinal gas and generating abnormal patterns of short-chain fatty acids than others; thus, the imbalance in gut microbiota may exacerbate the symptoms of bloating (King et al., 1998; O’Sullivan and O’Morain, 2000). The modification of microbiota attributed to probiotics may improve bloating symptoms by decreasing the production of intestinal gas and promoting gut motility. Despite the presence of ample data regarding this issue, the precise mechanism of action of specific probiotic species or strains in improving the symptoms of IBS is still speculative and remains to be confirmed.

It is notable that the efficacy of probiotic combinations are not necessarily better than mono-strain probiotics in present study, which was consistent with the outcomes of a research performed by Ringel-Kulka et al. (2014). Due to the different probiotic combinations used in many studies, it is difficult for us to determine which probiotic combination is more effective for IBS patients. Therefore, multi-center clinical trials with large sample sizes are still needed. Moreover, incorporating B. coagulans into a probiotic combination, or genetically engineering it to amplify its biological function may be a future research target to treat IBS patients.

Our NMA has several limitations. First, although we investigated all RCTs with synthesizable data, a lack of available trials or trials with large sample sizes for direct comparisons remains, which may have influenced our results. Second, due to the limited original data, we were unable to evaluate more clinical indicators, such as bowel habits, stool consistency, gut motility, serum inflammation-related factors, and the gut microbiome. Third, the methodologies of included RCTs vary in design, population, diagnosis criteria, IBS subtypes, and durations, and the outcome measures were different, making it difficult to draw robust conclusions. Therefore, the results of this NMA should be interpreted with caution.

Conclusions

The findings of our NMA suggest that B. coagulans was particularly effective in improving symptom relief rate, as well as global symptoms, abdominal pain, bloating, and straining scores. Furthermore, patients with IBS who received L. acidophilus had a lower incidence of AEs than those who received other treatments. Although some of the included RCTs are underpowered due to limited number of cases and different outcome measures, the results of our study may be useful in establishing treatment guidelines for IBS using probiotics, considering that there are only a few reports in the literature that have made direct comparisons between individual therapies for IBS.

Author Contributions

Guarantor of the article: LD is guarantor. Author contributions: TZ, CZ, JZ, FS, and LD conceived and drafted the study. CZ screened abstracts, TZ collected all data. TZ, CZ, JZ, and FS analyzed and interpreted the data. TZ and CZ drafted the manuscript. LD acquired the funding and performed critical revisions of the manuscript. All authors have approved the final draft of the manuscript.

Funding

This study was funded by and National Key R&D Program of China (2019YFA0905604) and National Natural Science Foundation of China (82170557).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Acknowledgments

We thank all authors who provided data for this network meta-analysis.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2022.859967/full#supplementary-material

Abbreviations

IBS, Irritable bowel syndrome; GI, gastrointestinal; IBS-D, diarrhea predominant IBS; RCTs, randomized controlled trials; QOL, quality of life; NMA, network meta-analysis; OR, odds ratio; 95% CI, 95% confidence interval; SMD, standardized mean difference; SUCRA, surface under the cumulative ranking curve; AEs, adverse events; USFDA, US Food and Drug Administration.

References

Abbas, Z., Yakoob, J., Jafri, W., Ahmad, Z., Azam, Z., Usman, M. W., et al. (2014). Cytokine and Clinical Response to Saccharomyces Boulardii Therapy in Diarrhea-Dominant Irritable Bowel Syndrome: A Randomized Trial. Eur. J. Gastroenterol. Hepatol. 26, 630–639. doi: 10.1097/MEG.0000000000000094

PubMed Abstract | CrossRef Full Text | Google Scholar

Agrawal, A., Houghton, L. A., Morris, J., Reilly, B., Guyonnet, D., Feuillerat., N., et al. (2009). Clinical Trial: The Effects of a Fermented Milk Product Containing Bifidobacterium Lactis DN-173 010 on Abdominal Distension and Gastrointestinal Transit in Irritable Bowel Syndrome With Constipation. Aliment. Pharmacol. Ther. 29, 104–114. doi: 10.1111/j.1365-2036.2008.03853.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Andresen, V., Gschossmann, J., Layer, P. (2020). Heat-Inactivated Bifidobacterium Bifidum MIMBb75 (SYN-HI-001) in the Treatment of Irritable Bowel Syndrome: A Multicentre, Randomised, Double-Blind, Placebo-Controlled Clinical Trial. Lancet Gastroenterol. Hepatol. 5, 658–666. doi: 10.1016/S2468-1253(20)30056-X

PubMed Abstract | CrossRef Full Text | Google Scholar

Bauserman, M., Michail, S. (2005). The Use of Lactobacillus GG in Irritable Bowel Syndrome in Children: A Double-Blind Randomized Control Trial. J. Pediatr. 147, 197–201. doi: 10.1016/j.jpeds.2005.05.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Begtrup, L. M., de Muckadell, O. B., Kjeldsen, J., Christensen, R. D., Jarbøl, D. E.. (2013). Long-Term Treatment With Probiotics in Primary Care Patients With Irritable Bowel Syndrome–a Randomised, Double-Blind, Placebo Controlled Trial. Scand. J. Gastroenterol. 48, 1127–1135. doi: 10.3109/00365521.2013.825314

PubMed Abstract | CrossRef Full Text | Google Scholar

Black, C. J., Ford, A. C. (2020). Global Burden of Irritable Bowel Syndrome: Trends, Predictions and Risk Factors. Nat. Rev. Gastroenterol. Hepatol. 17, 473–486. doi: 10.1038/s41575-020-0286-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Camilleri, M. (2021). Diagnosis and Treatment of Irritable Bowel Syndrome: A Review. JAMA 325, 865–877. doi: 10.1001/jama.2020.22532

PubMed Abstract | CrossRef Full Text | Google Scholar

Carabotti, M., Scirocco, A., Maselli, M. A., Severi, C.. (2015). The Gut-Brain Axis: Interactions Between Enteric Microbiota, Central and Enteric Nervous Systems. Ann. Gastroenterol. 28, 203–209.

PubMed Abstract | Google Scholar

Choi, C. H., Jo, S. Y., Park, H. J., Chang, S. K., Byeon, J. S., Myung, S. J.. (2011). A Randomized, Double-Blind, Placebo-Controlled Multicenter Trial of Saccharomyces Boulardii in Irritable Bowel Syndrome: Effect on Quality of Life. J. Clin. Gastroenterol. 45, 679–683. doi: 10.1097/MCG.0b013e318204593e

PubMed Abstract | CrossRef Full Text | Google Scholar

Creed, F., Ratcliffe, J., Fernandez, L., Tomenson, B., Palmer, S., Rigby, C., et al. (2001). Health-Related Quality of Life and Health Care Costs in Severe, Refractory Irritable Bowel Syndrome. Ann. Intern. Med. 134, 860–868. doi: 10.7326/0003-4819-134-9_part_2-200105011-00010

PubMed Abstract | CrossRef Full Text | Google Scholar

Dapoigny, M., Piche, T., Ducrotte, P., Lunaud, B., Cardot, J. M., Bernalier-Donadille, A.. (2012). Efficacy and Safety Profile of LCR35 Complete Freeze-Dried Culture in Irritable Bowel Syndrome: A Randomized, Double-Blind Study. World J. Gastroenterol. 18, 2067–2075. doi: 10.3748/wjg.v18.i17.2067

PubMed Abstract | CrossRef Full Text | Google Scholar

Drouault-Holowacz, S., Bieuvelet, S., Burckel, A., Cazaubiel, M., Dray, X., Marteau, P.. (2008). A Double Blind Randomized Controlled Trial of a Probiotic Combination in 100 Patients With Irritable Bowel Syndrome. Gastroenterol. Clin. Biol. 32, 147–152. doi: 10.1016/j.gcb.2007.06.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Ducrotté, P., Sawant, P., Jayanthi, V. (2012). Clinical Trial: Lactobacillus Plantarum 299v (DSM 9843) Improves Symptoms of Irritable Bowel Syndrome. World J. Gastroenterol. 18, 4012–4018. doi: 10.3748/wjg.v18.i30.4012

PubMed Abstract | CrossRef Full Text | Google Scholar

El-Serag, H. B., Olden, K. (2002). Health-Related Quality of Life Among Persons With Irritable Bowel Syndrome: A Systematic Review. Aliment. Pharmacol. Ther. 16, 1171–1185. doi: 10.1046/j.1365-2036.2002.01290.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Elshaghabee, F., Rokana, N., Gulhane, R. D., Sharma, C., Panwar, H.. (2017). Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives. Front. Microbiol. 8. doi: 10.3389/fmicb.2017.01490

CrossRef Full Text | Google Scholar

Enck, P., Zimmermann, K., Menke, G., Klosterhalfen, S.. (2009). Randomized Controlled Treatment Trial of Irritable Bowel Syndrome With a Probiotic E.-Coli Preparation (DSM17252) Compared to Placebo. Z Gastroenterol. 47, 209–214. doi: 10.1055/s-2008-1027702

PubMed Abstract | CrossRef Full Text | Google Scholar

Fond, G., Loundou, A., Hamdani, N., Boukouaci, W., Dargel, A., Oliveira, J., et al. (2014). Anxiety and Depression Comorbidities in Irritable Bowel Syndrome (IBS): A Systematic Review and Meta-Analysis. Eur. Arch. Psychiatry Clin. Neurosci. 264, 651–660. doi: 10.1007/s00406-014-0502-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Ford, A. C., Harris, L. A., Lacy, B. E., Quigley, E. M. M., Moayyedi, P.. (2018). Systematic Review With Meta-Analysis: The Efficacy of Prebiotics, Probiotics, Synbiotics and Antibiotics in Irritable Bowel Syndrome. Aliment. Pharmacol. Ther. 48, 1044–1060. doi: 10.1111/apt.15001

PubMed Abstract | CrossRef Full Text | Google Scholar

Fukudo, S., Okumura, T., Inamori, M., Okuyama, Y., Kanazawa, M., Kamiya, T., et al. (2021). Evidence-Based Clinical Practice Guidelines for Irritable Bowel Syndrome 2020. J. Gastroenterol. 56 (3), 193–217. doi: 10.1007/s00535-020-01746-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Gayathri, R., Aruna, T., Malar, S., Shilpa, B., Dhanasekar, K. R.. (2020). Efficacy of Saccharomyces Cerevisiae CNCM I-3856 as an Add-on Therapy for Irritable Bowel Syndrome. Int. J. Colorectal Dis. 35, 139–145. doi: 10.1007/s00384-019-03462-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Guglielmetti, S., Mora, D., Gschwender, M., Popp, K.. (2011). Randomised Clinical Trial: Bifidobacterium Bifidum MIMBb75 Significantly Alleviates Irritable Bowel Syndrome and Improves Quality of Life–A Double-Blind, Placebo-Controlled Study. Aliment. Pharmacol. Ther. 33, 1123–1132. doi: 10.1111/j.1365-2036.2011.04633.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Gupta, A. K., Maity, C. (2021). Efficacy and Safety of Bacillus Coagulans LBSC in Irritable Bowel Syndrome: A Prospective, Interventional, Randomized, Double-Blind, Placebo-Controlled Clinical Study [CONSORT Compliant]. Med. (Baltimore) 100, e23641. doi: 10.1097/MD.0000000000023641

CrossRef Full Text | Google Scholar

Guyonnet, D., Chassany, O., Ducrotte, P., Picard, C., Mouret, M., Mercier, C. H., et al. (2007). Effect of a Fermented Milk Containing Bifidobacterium Animalis DN-173 010 on the Health-Related Quality of Life and Symptoms in Irritable Bowel Syndrome in Adults in Primary Care: A Multicentre, Randomized, Double-Blind, Controlled Trial. Aliment. Pharmacol. Ther. 26, 475–486. doi: 10.1111/j.1365-2036.2007.03362.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Gwee, K. A., Gonlachanvit, S., Ghoshal, U. C., Chua, A. S. B., Miwa, H., Wu, J., et al. (2019). Second Asian Consensus on Irritable Bowel Syndrome. J. Neurogastroenterol. Motil. 25, 343–362. doi: 10.5056/jnm19041

PubMed Abstract | CrossRef Full Text | Google Scholar

Jafari, E., Vahedi, H., Merat, S., Momtahen, S., Riahi, A.. (2014). Therapeutic Effects, Tolerability and Safety of a Multi-Strain Probiotic in Iranian Adults With Irritable Bowel Syndrome and Bloating. Arch. Iran Med. 17, 466–470.

PubMed Abstract | Google Scholar

Kabir, M. A., Ishaque, S. M., Ali, M. S., Mahmuduzzaman, M., Hasan, M.. (2011). Role of Saccharomyces Boulardii in Diarrhea Predominant Irritable Bowel Syndrome. Mymensingh Med. J. 20, 397–401.

PubMed Abstract | Google Scholar

Kajander, K., Camilleri, M., McKinzie, S., Lempke, M. B., Burton, D. D., Thomforde, G. M., et al. (2005). A Probiotic Mixture Alleviates Symptoms in Irritable Bowel Syndrome Patients: A Controlled 6-Month Intervention. Aliment. Pharmacol. Ther. 22, 387–394. doi: 10.1111/j.1365-2036.2005.02579.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Kajander, K., Myllyluoma, E., Rajilić-Stojanović, M., Kyrönpalo, S., Rasmussen, M., Järvenpää, S., et al. (2008). Clinical Trial: Multispecies Probiotic Supplementation Alleviates the Symptoms of Irritable Bowel Syndrome and Stabilizes Intestinal Microbiota. Aliment. Pharmacol. Ther. 27, 48–57. doi: 10.1111/j.1365-2036.2007.03542.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, H. J., Camilleri, M., McKinzie, S., Lempke, M. B., Burton, D. D., Thomforde, G. M., et al. (2003). A Randomized Controlled Trial of a Probiotic, VSL#3, on Gut Transit and Symptoms in Diarrhoea-Predominant Irritable Bowel Syndrome. Aliment Pharmacol. Ther. 17, 895–904. doi: 10.1046/j.1365-2036.2003.01543.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, H. J., Roque, M. I. V., Camilleri, M., Stephens, D., Burton, D. D., Baxter, K., et al. (2005). A Randomized Controlled Trial of a Probiotic Combination VSL# 3 and Placebo in Irritable Bowel Syndrome With Bloating. Neurogastroenterol. Motil. 17, 687–696. doi: 10.1111/j.1365-2982.2005.00695.x

PubMed Abstract | CrossRef Full Text | Google Scholar

King, T. S., Elia, M., Hunter, J. O. (1998). Abnormal Colonic Fermentation in Irritable Bowel Syndrome. Lancet 352, 1187–1189. doi: 10.1016/s0140-6736(98)02146-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Kligler, B., Cohrssen, A. (2008). Probiotics. Am. Fam Physician. 78, 1073–1078. doi: 10.1007/s00384-011-1363-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Kruis, W., et al. (2012). A Double-Blind Placebo-Controlled Trial to Study Therapeutic Effects of Probiotic Escherichia Coli Nissle 1917 in Subgroups of Patients With Irritable Bowel Syndrome. Int. J. Colorectal Dis. 27, 467–474. doi: 10.1007/s00384-011-1363-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Lacy, B. E., Pimentel, M., Brenner, D. M., Chey, W. D., Keefer, L. A., Long, M. D., et al. (2021). ACG Clinical Guideline: Management of Irritable Bowel Syndrome. Am. J. Gastroenterol. 116, 17–44. doi: 10.14309/ajg.0000000000001036

PubMed Abstract | CrossRef Full Text | Google Scholar

Lacy, B. E., Patel, N. K. (2017). Rome Criteria and a Diagnostic Approach to Irritable Bowel Syndrome. J. Clin. Med. 6, 99. doi: 10.3390/jcm6110099

CrossRef Full Text | Google Scholar

Ligaarden, S. C., Axelsson, L., Naterstad, K., Lydersen, S., Farup, P. G.. (2010). A Candidate Probiotic With Unfavourable Effects in Subjects With Irritable Bowel Syndrome: A Randomised Controlled Trial. BMC Gastroenterol. 10, 16. doi: 10.1186/1471-230X-10-16

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, Y., Zhang, L., Wang, X., Wang, Z., Zhang, J., Jiang, R., et al. (2016). Similar Fecal Microbiota Signatures in Patients With Diarrhea-Predominant Irritable Bowel Syndrome and Patients With Depression. Clin. Gastroenterol. Hepatol. 14, 1602–1611.e5. doi: 10.1016/j.cgh.2016.05.033

PubMed Abstract | CrossRef Full Text | Google Scholar

Lyra, A., Hillilä, M., Huttunen, T., Männikkö, S., Taalikka, M., Tennilä, J., et al. (2016). Irritable Bowel Syndrome Symptom Severity Improves Equally With Probiotic and Placebo. World J. Gastroenterol. 22, 10631–10642. doi: 10.3748/wjg.v22.i48.10631

PubMed Abstract | CrossRef Full Text | Google Scholar

Madempudi, R. S., Ahire, J. J., Neelamraju, J., Tripathi, A., Nanal, S.. (2019). Randomized Clinical Trial: The Effect of Probiotic Bacillus Coagulans Unique IS2 vs. Placebo on the Symptoms Management of Irritable Bowel Syndrome in Adults. Sci. Rep. 9, 12210. doi: 10.1038/s41598-019-48554-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Maity, C., Gupta, A. K., Saroj, D. B., Biyani, A., Bagkar, P., Kulkarni, J., et al. (2020). Impact of a Gastrointestinal Stable Probiotic Supplement Bacillus Coagulans LBSC on Human Gut Microbiome Modulation. J. Diet Suppl.18, 577–596. doi: 10.1080/19390211.2020.1814931

CrossRef Full Text | Google Scholar

Majeed, M., Nagabhushanam, K., Natarajan, S., Sivakumar, A., Ali, F., Pande, A., et al. (2016). Bacillus Coagulans MTCC 5856 Supplementation in the Management of Diarrhea Predominant Irritable Bowel Syndrome: A Double Blind Randomized Placebo Controlled Pilot Clinical Study. Nutr. J. 15, 21. doi: 10.1186/s12937-016-0140-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Martoni, C. J., Srivastava, S., Leyer, G. J. (2020). Lactobacillus Acidophilus DDS-1 and Bifidobacterium Lactis UABla-12 Improve Abdominal Pain Severity and Symptomology in Irritable Bowel Syndrome: Randomized Controlled Trial. Nutrients 12, 363. doi: 10.3390/nu12020363

CrossRef Full Text | Google Scholar

Mearin, F., Lacy, B. E., Chang, L., Chey, W. D., Lembo, A. J., Simren, M., et al. (2016). Bowel Disorders. Gastroenterology S0016–5085(16)00222-5. doi: 10.1053/j.gastro.2016.02.031

PubMed Abstract | CrossRef Full Text | Google Scholar

Moayyedi, P., Andrews, C. N., MacQueen, G., Korownyk, C., Marsiglio, M., Graff, L., et al. (2019). Canadian Association of Gastroenterology Clinical Practice Guideline for the Management of Irritable Bowel Syndrome (IBS). J. Can. Assoc. Gastroenterol. 2, 6–29. doi: 10.1093/jcag/gwy071

PubMed Abstract | CrossRef Full Text | Google Scholar

Mu, Y., Cong, Y. (2019). Bacillus Coagulans and its Applications in Medicine. Benef. Microbes 10, 679–688. doi: 10.3920/BM2019.0016

PubMed Abstract | CrossRef Full Text | Google Scholar

Ng, Q. X., et al. (2018). A Meta-Analysis of the Use of Probiotics to Alleviate Depressive Symptoms. J. Affect. Disord. 228, 13–19. doi: 10.1016/j.jad.2017.11.063

PubMed Abstract | CrossRef Full Text | Google Scholar

Niedzielin, K., Kordecki, H., Birkenfeld, B. (2001). A Controlled, Double-Blind, Randomized Study on the Efficacy of Lactobacillus Plantarum 299V in Patients With Irritable Bowel Syndrome. Eur. J. Gastroenterol. Hepatol. 13, 1143–1147. doi: 10.1097/00042737-200110000-00004

PubMed Abstract | CrossRef Full Text | Google Scholar

Niv, E., Naftali, T., Hallak, R., Vaisman, N.. (2005). The Efficacy of Lactobacillus Reuteri ATCC 55730 in the Treatment of Patients With Irritable Bowel Syndrome–A Double Blind, Placebo-Controlled, Randomized Study. Clin. Nutr. 24, 925–931. doi: 10.1016/j.clnu.2005.06.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Oka, P., Parr, H., Barberio, B., Black, C. J., Savarino, E. V., Ford, A. C.. (2020). Global Prevalence of Irritable Bowel Syndrome According to Rome III or IV Criteria: A Systematic Review and Meta-Analysis. Lancet Gastroenterol. Hepatol. 5, 908–917. doi: 10.1016/S2468-1253(20)30217-X

PubMed Abstract | CrossRef Full Text | Google Scholar

O’Mahony, L., McCarthy, J., Kelly, P., Hurley, G., Luo, F., Chen, K., et al. (2005). Lactobacillus and Bifidobacterium in Irritable Bowel Syndrome: Symptom Responses and Relationship to Cytokine Profiles. Gastroenterology 128, 541–551. doi: 10.1053/j.gastro.2004.11.050

PubMed Abstract | CrossRef Full Text | Google Scholar

O’Sullivan, M. A., O’Morain, C. A. (2000). Bacterial Supplementation in the Irritable Bowel Syndrome. A Randomised Double-Blind Placebo-Controlled Crossover Study. Dig. Liver Dis. 32, 294–301. doi: 10.1016/s1590-8658(00)80021-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Page, M. J., McKenzie, J. E., Bossuyt, P. M., Boutron, I., Hoffmann, T. C., Mulrow, C. D., et al. (2021). The PRISMA 2020 Statement: An Updated Guideline for Reporting Systematic Reviews. PloS Med. 18, e1003583. doi: 10.1371/journal.pmed.1003583

PubMed Abstract | CrossRef Full Text | Google Scholar

Pineton, D. C. G., Neut, C., Chau, A., Cazaubiel, M., Pelerin, F., Justen, P., et al. (2015). A Randomized Clinical Trial of Saccharomyces Cerevisiae Versus Placebo in the Irritable Bowel Syndrome. Dig. Liver Dis. 47, 119–124. doi: 10.1016/j.dld.2014.11.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Pinto-Sanchez, M. I., Hall, G. B., Ghajar, K., Nardelli, A., Bolino, C., Lau, J. T., et al. (2017). Probiotic Bifidobacterium Longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome. Gastroenterology 153, 448–459.e8. doi: 10.1053/j.gastro.2017.05.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Plaza-Diaz, J., Gomez-Llorente, C., Fontana, L., Gil, A. (2014). Modulation of Immunity and Inflammatory Gene Expression in the Gut, in Inflammatory Diseases of the Gut and in the Liver by Probiotics. World J. Gastroenterol. 20, 15632–15649. doi: 10.3748/wjg.v20.i42.15632

PubMed Abstract | CrossRef Full Text | Google Scholar

Preston, K., Krumian, R., Hattner, J., de Montigny, D., Stewart, M., Gaddam, S. (2018). Lactobacillus Acidophilus CL1285, Lactobacillus Casei LBC80R and Lactobacillus Rhamnosus CLR2 Improve Quality-of-Life and IBS Symptoms: A Double-Blind, Randomised, Placebo-Controlled Study. Benef. Microbes 9, 697–706. doi: 10.3920/BM2017.0105

PubMed Abstract | CrossRef Full Text | Google Scholar

Qin, J., Li, R., Raes, J., Arumugam, M., Burgdorf, K. S., Manichanh, C., et al. (2010). A Human Gut Microbial Gene Catalogue Established by Metagenomic Sequencing. Nature 464, 59–65. doi: 10.1038/nature08821

PubMed Abstract | CrossRef Full Text | Google Scholar

Raskov, H., Burcharth, J., Pommergaard, H. C., Rosenberg, J. (2016). Irritable Bowel Syndrome, the Microbiota and the Gut-Brain Axis. Gut Microbes 7, 365–383. doi: 10.1080/19490976.2016.1218585

PubMed Abstract | CrossRef Full Text | Google Scholar

Ringel-Kulka, T., Goldsmith, J. R., Carroll, I. M., Barros, S. P., Palsson, O., Jobin, C., et al. (2014). Lactobacillus Acidophilus NCFM Affects Colonic Mucosal Opioid Receptor Expression in Patients With Functional Abdominal Pain - a Randomised Clinical Study. Aliment. Pharmacol. Ther. 40, 200–207. doi: 10.1111/apt.12800

PubMed Abstract | CrossRef Full Text | Google Scholar

Ringel, Y., Ringel-Kulka, T. (2015). The Intestinal Microbiota and Irritable Bowel Syndrome. J. Clin. Gastroenterol. 49 Suppl 1, S56–S59. doi: 10.1097/MCG.0000000000000418

PubMed Abstract | CrossRef Full Text | Google Scholar

Roberts, L. M., McCahon, D., Holder, R., Wilson, S., Hobbs, F. D. (2013). A Randomised Controlled Trial of a Probiotic ‘Functional Food’ in the Management of Irritable Bowel Syndrome. BMC Gastroenterol. 13, 45. doi: 10.1186/1471-230X-13-45

PubMed Abstract | CrossRef Full Text | Google Scholar

Rousseaux, C., Thuru, X., Gelot, A., Barnich, N., Neut, C., Dubuquoy, L., et al. (2007). Lactobacillus Acidophilus Modulates Intestinal Pain and Induces Opioid and Cannabinoid Receptors. Nat. Med. 13, 35–37. doi: 10.1038/nm1521

PubMed Abstract | CrossRef Full Text | Google Scholar

Søndergaard, B., Olsson, J., Ohlson, K., Svensson, U., Bytzer, P., Ekesbo, R. (2011). Effects of Probiotic Fermented Milk on Symptoms and Intestinal Flora in Patients With Irritable Bowel Syndrome: A Randomized, Placebo-Controlled Trial. Scand. J. Gastroenterol. 46, 663–672. doi: 10.3109/00365521.2011.565066

PubMed Abstract | CrossRef Full Text | Google Scholar

Sadrin, S., Sennoune, S., Gout, B., Marque, S., Moreau, J., Zinoune, K., et al. (2020). A 2-Strain Mixture of Lactobacillus Acidophilus in the Treatment of Irritable Bowel Syndrome: A Placebo-Controlled Randomized Clinical Trial. Dig. Liver Dis. 52, 534–540. doi: 10.1016/j.dld.2019.12.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmidt, C. (2015). Mental Health: Thinking From the Gut. Nature 518, S12–S15. doi: 10.1038/518S13a

PubMed Abstract | CrossRef Full Text | Google Scholar

Sinn, D. H., Song, J. H., Kim, H. J., Lee, J. H., Son, H. J., Chang, D. K., et al. (2008). Therapeutic Effect of Lactobacillus Acidophilus-SDC 2012, 2013 in Patients With Irritable Bowel Syndrome. Dig. Dis. Sci. 53, 2714–2718. doi: 10.1007/s10620-007-0196-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Spiegel, B. M., Gralnek, I. M., Bolus, R., Chang, L., Dulai, G. S., Mayer, E. A., et al. (2004). Clinical Determinants of Health-Related Quality of Life in Patients With Irritable Bowel Syndrome. Arch. Intern. Med. 164, 1773–1780. doi: 10.1001/archinte.164.16.1773

PubMed Abstract | CrossRef Full Text | Google Scholar

Spiller, R., Pelerin, F., Cayzeele, D. A., Maudet, C., Housez, B., Cazaubiel, M., et al. (2016). Randomized Double Blind Placebo-Controlled Trial of Saccharomyces Cerevisiae CNCM I-3856 in Irritable Bowel Syndrome: Improvement in Abdominal Pain and Bloating in Those With Predominant Constipation. U. Eur. Gastroenterol. J. 4, 353–362. doi: 10.1177/2050640615602571

CrossRef Full Text | Google Scholar

Stevenson, C., Blaauw, R., Fredericks, E., Visser, J., Roux, S.. (2014). Randomized Clinical Trial: Effect of Lactobacillus Plantarum 299 V on Symptoms of Irritable Bowel Syndrome. Nutrition 30, 1151–1157. doi: 10.1016/j.nut.2014.02.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Sudha, M. R., Jayanthi, N., Aasin, M., Dhanashri, R. D., Anirudh, T. (2018). Efficacy of Bacillus Coagulans Unique IS2 in Treatment of Irritable Bowel Syndrome in Children: A Double Blind, Randomised Placebo Controlled Study. Benef. Microbes 9, 563–572. doi: 10.3920/BM2017.0129

PubMed Abstract | CrossRef Full Text | Google Scholar

Sun, Y. Y., Li, M., Li, Y. Y., Li, L. X., Zhai, W. Z., Wang, P. (2018). The Effect of Clostridium Butyricum on Symptoms and Fecal Microbiota in Diarrhea-Dominant Irritable Bowel Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial. Sci. Rep. 8, 2964. doi: 10.1038/s41598-018-21241-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Thijssen, A. Y., Li, M., Li, Y. Y., Li, L. X., Zhai, W. Z., Wang, P. (2016). Efficacy of Lactobacillus Casei Shirota for Patients With Irritable Bowel Syndrome. Eur. J. Gastroenterol. Hepatol. 28, 8–14. doi: 10.1097/MEG.0000000000000484

PubMed Abstract | CrossRef Full Text | Google Scholar

Vasant, D. H., Paine, P. A., Black, C. J., Houghton, L. A., Everitt, H. A., Corsetti, M., et al. (2021). British Society of Gastroenterology Guidelines on the Management of Irritable Bowel Syndrome. Gut 70, 1214–1240. doi: 10.1136/gutjnl-2021-324598

PubMed Abstract | CrossRef Full Text | Google Scholar

Whorwell, P. J., Altringer, L., Morel, J., Bond, Y., Charbonneau, D., O'Mahony, L., et al. (2006). Efficacy of an Encapsulated Probiotic Bifidobacterium Infantis 35624 in Women With Irritable Bowel Syndrome. Am. J. Gastroenterol. 101, 1581–1590. doi: 10.1111/j.1572-0241.2006.00734.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Wilkins, T., Sequoia, J. (2017). Probiotics for Gastrointestinal Conditions: A Summary of the Evidence. Am. Fam Physician. 96, 170–178. doi: 10.1007/s10620-014-3299-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Wong, R. K., Yang, C., Song, G. H., Wong, J., Ho, K. Y. (2015). Melatonin Regulation as a Possible Mechanism for Probiotic (VSL#3) in Irritable Bowel Syndrome: A Randomized Double-Blinded Placebo Study. Dig. Dis. Sci. 60, 186–194. doi: 10.1007/s10620-014-3299-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Yano, J. M., Yu, K., Donaldson, G. P., Shastri, G. G., Ann, P., Ma, L., et al. (2015). Indigenous Bacteria From the Gut Microbiota Regulate Host Serotonin Biosynthesis. Cell 161, 264–276. doi: 10.1016/j.cell.2015.02.047

PubMed Abstract | CrossRef Full Text | Google Scholar

Zeng, J., Li, Y. Q., Zuo, X. L., Zhen, Y. B., Yang, J., Liu, C. H. (2008). Clinical Trial: Effect of Active Lactic Acid Bacteria on Mucosal Barrier Function in Patients With Diarrhoea-Predominant Irritable Bowel Syndrome. Aliment. Pharmacol. Ther. 28, 994–1002. doi: 10.1111/j.1365-2036.2008.03818.x

PubMed Abstract | CrossRef Full Text | Google Scholar