Abstract
Endothelial damage and vascular pathology have been recognized as major features of COVID-19 since the beginning of the pandemic. Two main theories regarding how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) damages endothelial cells and causes vascular pathology have been proposed: direct viral infection of endothelial cells or indirect damage mediated by circulating inflammatory molecules and immune mechanisms. However, these proposed mechanisms remain largely untested in vivo. In the present study, we utilized a set of new mouse genetic tools developed in our lab to test both the necessity and sufficiency of endothelial human angiotensin-converting enzyme 2 (hACE2) in COVID-19 pathogenesis. Our results demonstrate that endothelial ACE2 and direct infection of vascular endothelial cells do not contribute significantly to the diverse vascular pathology associated with COVID-19.
Introduction
The most common clinical feature reported in patients with COVID-19 is respiratory symptoms (1, 2). In addition to primarily causing pulmonary symptoms, COVID-19 disease is accompanied by vascular pathology, endothelial damage, and vascular coagulopathy (3–5). Reports emerged around the world confirming a disproportionate prevalence of abnormal thrombotic events and vascular pathology in patients with COVID-19, even in those not in intensive care units (6–14). Theories regarding the mechanism of vascular disease observed in patients with COVID-19 have been proposed, including direct infection of endothelial cells and systemic inflammatory responses (15–22). However, these hypotheses remain largely untested, and the cellular basis of vascular pathology remains controversial. In this study, we used a set of new mouse genetic tools (23) to rigorously test endothelial contribution to COVID-19-associated vascular pathology.
Materials and methods
Mice
hACE2fl/y mice and LSL-hACE2+/0 mice have been generated through CRISPR/Cas9-assisted mouse embryonic stem cell targeting and have been described (23). Briefly, for loss of function mouse line, the human ACE2 cDNA sequence was inserted after the ATG-start codon of mouse Ace2 in exon2, flanking the polyA cassette with loxP sites, to achieve Cre-mediated cell type-specific deletion of ACE2. For gain of function mouse line, the human ACE2 cDNA sequence was targeted to Rosa26 locus with Lox-STOP-Lox cassette to permit tissue-specific gain of expression of ACE2. Tie2-Cre transgenic mice have been used for tissue-specific drivers as previously described (24). All mice were maintained on a mixed genetic background, including C57BL/8 and other strains, at the University of Pennsylvania animal facility. Mice were genotyped by PCR as described (23). The number of male mice used in each experiment ranged from three to nine.
Viral inoculation and tissue harvest
Viral inoculations were performed as described previously (23). Briefly, mice were anesthetized with isoflurane and then intranasally infected with SARS-CoV-2 (Isolate USA-WA1/2020; BEI resources: NR-52281) that was obtained from BEI Resource. Mice were monitored and weighed daily, then euthanized at a humane endpoint when they lost 20% of their starting weights. Mice studies were combined results from Penn ABSL3 laboratory and Cornell ABSL3 laboratory in accordance with protocols approved by the IACUC at the University of Pennsylvania and Cornell University. For tissue harvest, mice were euthanized with ketamine/xylazine. Lungs were gently inflated with PBS infusion via trachea cannulation. Then lungs were fixed in 4% paraformaldehyde with a minimum of 72 h to ensure viral inactivation. Tissues were removed from the animal BSL3 facility, followed by ethanol dehydration and embedding in paraffin blocks for histology. Hematoxylin and eosin staining was performed on paraffin sections.
Immunofluorescence staining and analysis
Immunohistochemistry staining was performed as previously described (23) with control and experimental samples on the same slide and under identical staining conditions. Primary antibodies were as follows: pan-ACE2 (goat, 1:1,000, R&D AF933), hACE2 (rabbit, 1:200, Abcam ab108209), SARS-CoV-2 nucleocapsid (rabbit, 1:500, Rockland 200-401-A50), ICAM-1 (rabbit, 1:500, Abcam ab179707), vWF (rabbit 1:1,000, Novus Biologicals NB600-586), and PECAM (goat 1:500, R&D AF3628). Fluorescence-conjugated Alexa Fluor secondary antibodies were used (1:500, Invitrogen) according to the primary antibody species and counterstained with DAPI (1:1,000). ICAM1 and vWF fluorescence intensity were calculated by integrated fluorescence intensity. All images were analyzed using ImageJ/FIJI software.
Statistics
Mice were inoculated with SARS-CoV-2 in a blinded fashion without knowledge of genotypes, and infections were performed in two different ABSL-3 facilities with independent experimenters. Statistical tests used to determine significance are described in the figure legends. GraphPad Prism 9.5.1 was used to generate graphs and statistical analyses. Survival curve statistics were performed with log-rank Mantel-Cox tests. All t-tests performed were two-tailed.
Results and discussion
Cellular expression of ACE2 is indispensable for SARS-CoV-2 infection in pneumocytes (25, 26), but SARS-CoV-2 is unable to bind mouse ACE2. To determine if endothelial cells directly contribute to lethal infection, we generated animals that express human ACE2 (hACE2) from the mouse Ace2 locus in a manner that enables cell-specific loss of hACE2 using Cre recombinase (hACE2fl/y mice) (23). We crossed hACE2fl/y mice onto a Tie2-Cre transgenic mouse line that drives Cre expression in endothelial cells (ECs) to generate mice that express hACE2 in all cells except vascular ECs. hACE2fl/y; Tie2-Cre+ mice and control littermates were exposed to 105 PFU of SARS-CoV-2 virus via nasal inhalation. hACE2fl/y; Tie2-Cre+ mice showed no significant difference in survival after exposure to SARS-CoV-2 compared with the littermate controls (Figure 1A). Histological analysis revealed the presence of alveolar infiltrates and pulmonary vascular thrombi in the lungs of infected hACE2fl/y; Tie2-Cre+ mice that were indistinguishable from findings observed in control hACE2fl/y mice (Figure 1B). Histological analysis using hematoxylin-eosin staining of tissue sections from the small intestine, kidney, liver, and heart also failed to identify any vascular pathology (Supplementary Figure S1). Next, we evaluated the expression of inflammation-induced protein intracellular adhesion marker 1 (ICAM1) and the pro-coagulant, inflammation-induced protein Von Willebrand factor (vWF) in the mice following the SARS-CoV-2 infection, given both ICAM1 and vWF have been closely associated with COVID-19 induced vascular damage (27, 28). Expression of ICAM1 and vWF were also similar in the lung capillary endothelial cells of SARS-CoV-2-infected hACE2fl/y and hACE2fl/y; Tie2-Cre+ mice (Figures 1C,D).
Figure 1
The studies described above suggested that endothelial cell infection is not required for vascular COVID-19 pathology when hACE2 is expressed at endogenous levels. In fact, immunostaining of lung sections using anti-ACE2 antibodies was able to detect ACE2 expression in epithelial but not endothelial cells (23) (Figure 1E). To more rigorously test the role of endothelial hACE2, we next crossed Tie2-Cre onto a recently described Cre-activated gain of function hACE2 allele (loxP-stop-loxP-hACE2 or LSL-hACE2+/0) (23) to over-express hACE2 in vascular endothelial cells. Tie2-Cre;LSL-hACE2+/0 animals exhibited very high endothelial-specific expression of hACE2, assessed by immunostaining of tissue sections compared with hACE2fl/y mice (Figure 1F). To ensure that Tie2-Cre;LSL-hACE2+/0 animals would be productively infected following SARS-CoV-2 exposure, we generated Tie2-Cre;LSL-hACE2+/0;hACE2fl/y animals that support robust infection of the nasal and respiratory epithelium (23) (Figure 1F). Despite high levels of endothelial hACE2 expression, we failed to detect nucleocapsid protein that colocalized with PECAM+ endothelial cells following nasal SARS-CoV-2 infection (Figure 1F). In contrast, we have previously shown that this gain of function allele is sufficient to drive hACE2 expression and support SARS-CoV-2 infection in both neuronal cells and lung epithelial cells (23). These studies support the conclusion that SARS-CoV-2 does not confer endothelial cell damage and vascular thrombosis through direct viral infection of those cells. They further demonstrate that the levels of circulating virus are too low to infect even endothelial cells that express very high levels of hACE2, and therefore that most COVID-19 pathology arises due to aerosol infection of the nasal and pulmonary epithelium.
It has been debated whether direct viral infection of endothelial cells or indirect damage from systematic inflammation underlie COVID-19-associated vascular pathology (3). Our murine vascular endothelial loss and gain of function studies reported here provide strong in vivo evidence that endothelial ACE2 and direct infection of vascular endothelial cells do not contribute significantly to the diverse vascular pathology associated with COVID-19. These findings are consistent with previously reported in vitro studies that showed human endothelial cells are not readily infected by SARS-CoV-2 (21). Together with our recently reported studies, these findings strongly support a mechanism in which SARS-CoV-2 infection of nasal epithelial and neuronal cells stimulates a powerful inflammatory response that is the cause of COVID-19 vascular pathology.
Limitations of the study
In the present study, we utilized both loss of function and gain of function hACE2 mouse lines and demonstrated that direct endothelial viral infection does not contribute to COVID-19-associated vascular pathology. Future studies are needed to define the cytokines that likely drive secondary vascular inflammation and thrombosis and to understand the molecular mechanism by which systemic inflammation damages endothelium following SARS-CoV-2 infection. We used the original isolate SARS-CoV-2 USA-WA1/2020 strain in our study because that isolate is the best characterized regarding vascular complication. Omicron BA.1 variant failed to confer lethal disease and associated vascular phenotypes in our mouse models (23). Future studies testing the impact of other variants on the vascular system will be needed. We performed our studies on male mice due to the Ace2 allele being located on the X chromosome, enabling a straightforward comparison. Mouse models are not humans, and our mouse model hACE2fl/y expresses a higher level of hACE2 as previously reported (23). Thus there are likely to be differences in pathogenic mechanisms identified using our model compared to human studies. However, this difference should bias toward rather than against a direct endothelial infection mechanism and it does not weaken our negative conclusions. Future studies looking at longer-term vascular events in mice with lower levels of hACE2 expression will be needed to address non-acute mechanisms of COVID-19-related cardiovascular disease.
Statements
Data availability statement
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.
Ethics statement
The animal study was approved by University of Pennsylvania IACUC. The study was conducted in accordance with the local legislation and institutional requirements.
Author contributions
MK: Conceptualization, Formal analysis, Funding acquisition, Project administration, Writing – original draft. SG: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – original draft. AT: Conceptualization, Formal analysis, Writing – original draft. MW: Conceptualization, Data curation, Formal analysis, Methodology, Writing – original draft. DB: Conceptualization, Data curation, Writing – original draft. BI: Data curation, Formal analysis, Conceptualization, Writing – original draft. JY: Data curation, Formal analysis, Writing – original draft. XC: Conceptualization, Data curation, Formal analysis, Writing – original draft. PM: Methodology, Project administration, Writing – original draft. NL: Data curation, Formal analysis, Methodology, Writing – original draft. SS: Methodology, Project administration, Writing – original draft. AA: Project administration, Data curation, Methodology, Writing – original draft. KJ: Data curation, Methodology, Project administration, Investigation, Writing – original draft. EM: Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Writing – original draft, Conceptualization. HA: Conceptualization, Data curation, Formal analysis, Funding acquisition, Project administration, Writing – original draft. PH: Formal analysis, Writing – original draft.
Funding
The author(s) declare that no financial support was received for the research, authorship, and/or publication of this article.
This work was supported by National Institute of Health grants R01HL39552-04S1 (MLK), R01HL164929 (MLK and EEM), AHA 963048 (MLK), R01AI109022 and R21AI156731 (HAC), and T32EB023860 (DWB), an AHA Postdoctoral Fellowship 906488 (SG), and a Penn CVI Dream Team grant (MK).
Acknowledgments
We thank Kahn lab members for their helpful discussions.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1266276/full#supplementary-material
References
1.
ZhouPYangXLWangXGHuBZhangLZhangWet alA pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. (2020) 579:270–3. 10.1038/s41586-020-2012-7
2.
HuangCWangYLiXRenLZhaoJHuYet alClinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. (2020) 395:497–506. 10.1016/S0140-6736(20)30183-5
3.
TeuwenLAGeldhofVPasutACarmelietP. COVID-19: the vasculature unleashed. Nat Rev Immunol. (2020) 20:389–91. 10.1038/s41577-020-0343-0
4.
SmadjaDMMentzerSJFontenayMLaffanMAAckermannMHelmsJet alCOVID-19 is a systemic vascular hemopathy: insight for mechanistic and clinical aspects. Angiogenesis. (2021) 24:755–88. 10.1007/s10456-021-09805-6
5.
ConnorsJMLevyJH. COVID-19 and its implications for thrombosis and anticoagulation. Blood. (2020) 135:2033–40. 10.1182/blood.2020006000
6.
ZhouFYuTDuRFanGLiuYLiuZet alClinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. Lancet. (2020) 395:1054–62. 10.1016/S0140-6736(20)30566-3
7.
TangNLiDWangXSunZ. Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia. J Thromb Haemost. (2020) 18:844–7. 10.1111/jth.14768
8.
OxleyTJMoccoJMajidiSKellnerCPShoirahHSinghIPet alLarge-vessel stroke as a presenting feature of COVID-19 in the young. N Engl J Med. (2020) 382:e60. 10.1056/NEJMc2009787
9.
KlokFAKruipMvan der MeerNJMArbousMSGommersDKantKMet alConfirmation of the high cumulative incidence of thrombotic complications in critically ill ICU patients with COVID-19: an updated analysis. Thromb Res. (2020) 191:148–50. 10.1016/j.thromres.2020.04.041
10.
SongWCFitzGeraldGA. COVID-19, microangiopathy, hemostatic activation, and complement. J Clin Invest. (2020) 130:3950–3. 10.1172/JCI140183
11.
GoshuaGPineABMeizlishMLChangCHZhangHBahelPet alEndotheliopathy in COVID-19-associated coagulopathy: evidence from a single-centre, cross-sectional study. Lancet Haematol. (2020) 7:e575–82. 10.1016/S2352-3026(20)30216-7
12.
Al-SamkariHKarp LeafRSDzikWHCarlsonJCTFogertyAEWaheedAet alCOVID-19 and coagulation: bleeding and thrombotic manifestations of SARS-CoV-2 infection. Blood. (2020) 136:489–500. 10.1182/blood.2020006520
13.
AckermannMVerledenSEKuehnelMHaverichAWelteTLaengerFet alPulmonary vascular endothelialitis, thrombosis, and angiogenesis in COVID-19. N Engl J Med. (2020) 383:120–8. 10.1056/NEJMoa2015432
14.
JimenezDGarcia-SanchezARaliPMurielABikdeliBRuiz-ArtachoPet alIncidence of VTE and bleeding among hospitalized patients with coronavirus disease 2019: a systematic review and meta-analysis. Chest. (2021) 159:1182–96. 10.1016/j.chest.2020.11.005
15.
MerrillJTErkanDWinakurJJamesJA. Emerging evidence of a COVID-19 thrombotic syndrome has treatment implications. Nat Rev Rheumatol. (2020) 16:581–9. 10.1038/s41584-020-0474-5
16.
HeLMäeMAMuhlLSunYPietiläRNaharKet alPericyte-specific vascular expression of SARS-CoV-2 receptor ACE2—implications for microvascular inflammation and hypercoagulopathy in COVID-19. bioRxiv. 2020:2020.2005.2011.088500. 10.1101/2020.05.11.088500
17.
HenryBMVikseJBenoitSFavaloroEJLippiG. Hyperinflammation and derangement of renin-angiotensin-aldosterone system in COVID-19: a novel hypothesis for clinically suspected hypercoagulopathy and microvascular immunothrombosis. Clin Chim Acta. (2020) 507:167–73. 10.1016/j.cca.2020.04.027
18.
GuSXTyagiTJainKGuVWLeeSHHwaJMet alThrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation. Nat Rev Cardiol. (2020) 18(3):194–209. 10.1038/s41569-020-00469-1
19.
PericoLBenigniACasiraghiFNgLFPReniaLRemuzziG. Immunity, endothelial injury and complement-induced coagulopathy in COVID-19. Nat Rev Nephrol. (2021) 17:46–64. 10.1038/s41581-020-00357-4
20.
SchimmelLChewKYStocksCJYordanovTEEssebierPKulasingheAet alEndothelial cells are not productively infected by SARS-CoV-2. Clin Transl Immunology. (2021) 10:e1350. 10.1002/cti2.1350
21.
McCrackenIRSagincGHeLHuseynovADanielsAFletcherSet alLack of evidence of angiotensin-converting enzyme 2 expression and replicative infection by SARS-CoV-2 in human endothelial cells. Circulation. (2021) 143:865–8. 10.1161/CIRCULATIONAHA.120.052824
22.
NicosiaRFLigrestiGCaporarelloNAkileshSRibattiD. COVID-19 vasculopathy: mounting evidence for an indirect mechanism of endothelial injury. Am J Pathol. (2021) 191:1374–84. 10.1016/j.ajpath.2021.05.007
23.
TangATBuchholzDWSzigetyKMImbiakhaBGaoSFrankfurterMet alCell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection. PLoS Biol. (2023) 21:e3001989. 10.1371/journal.pbio.3001989
24.
KisanukiYYHammerREMiyazakiJWilliamsSCRichardsonJAYanagisawaM. Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Dev Biol. (2001) 230:230–42. 10.1006/dbio.2000.0106
25.
OuditGYWangKViveirosAKellnerMJPenningerJM. Angiotensin-converting enzyme 2-at the heart of the COVID-19 pandemic. Cell. (2023) 186:906–22. 10.1016/j.cell.2023.01.039
26.
DeloreyTMZieglerCGKHeimbergGNormandRYangYSegerstolpeAet alCOVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets. Nature. (2021) 595:107–13. 10.1038/s41586-021-03570-8
27.
NagashimaSMendesMCCamargo MartinsAPBorgesNHGodoyTMMiggiolaroAet alEndothelial dysfunction and thrombosis in patients with COVID-19-brief report. Arterioscler Thromb Vasc Biol. (2020) 40:2404–7. 10.1161/ATVBAHA.120.314860
28.
PhilippeAChocronRGendronNBoryOBeauvaisAPeronNet alCirculating Von willebrand factor and high molecular weight multimers as markers of endothelial injury predict COVID-19 in-hospital mortality. Angiogenesis. (2021) 24:505–17. 10.1007/s10456-020-09762-6
Summary
Keywords
SARS-CoV-2, endothelial cell, COVID-19, vascular, hACE2
Citation
Gao S, Tang AT, Wang M, Buchholz DW, Imbiakha B, Yang J, Chen X, Hewins P, Mericko-Ishizuka P, Leu NA, Sterling S, August A, Jurado KA, Morrisey EE, Aguilar-Carreno H and Kahn ML (2023) Endothelial SARS-CoV-2 infection is not the underlying cause of COVID-19-associated vascular pathology in mice. Front. Cardiovasc. Med. 10:1266276. doi: 10.3389/fcvm.2023.1266276
Received
24 July 2023
Accepted
05 September 2023
Published
26 September 2023
Volume
10 - 2023
Edited by
Masanori Aikawa, Harvard Medical School, United States
Reviewed by
Antonin Trimaille, Hôpitaux Universitaires de Strasbourg, France Nicolas Gendron, Hôpital Européen Georges Pompidou, France
Updates
Copyright
© 2023 Gao, Tang, Wang, Buchholz, Imbiakha, Yang, Chen, Hewins, Mericko-Ishizuka, Leu, Sterling, August, Jurado, Morrisey, Aguilar-Carreno and Kahn.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Mark L. Kahn markkahn@pennmedicine.upenn.edu
†These authors share first authorship
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.