Original Research ARTICLE
CRTAM Protects Against Intestinal Dysbiosis During Pathogenic Parasitic Infection by Enabling Th17 Maturation
- 1Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, United States
- 2Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, United States
- 3Division of Gastroenterology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, United States
- 4Division of Rheumatology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, United States
The gastrointestinal tract hosts the largest collection of commensal microbes in the body. Infections at this site can cause significant perturbations in the microbiota, known as dysbiosis, that facilitate the expansion of pathobionts, and can elicit inappropriate immune responses that impair the intestinal barrier function. Dysbiosis typically occurs during intestinal infection with Toxoplasma gondii. Host resistance to T. gondii depends on a potent Th1 response. In addition, a Th17 response is also elicited. How Th17 cells contribute to the host response to T. gondii remains unclear. Here we show that class I-restricted T cell-associated molecule (CRTAM) expression on T cells is required for an optimal IL-17 production during T. gondii infection. Moreover, that the lack of IL-17, results in increased immunopathology caused by an impaired antimicrobial peptide production and bacterial translocation from the intestinal lumen to the mesenteric lymph nodes and spleen.
The gastrointestinal tract hosts the largest collection of commensal microbes in the body, which impact host metabolism as well as development and regulation of the immune system (1). Pathogenic infections at this site can cause significant perturbations in the microbiota, known as dysbiosis, that facilitate the expansion of pathobionts, elicit inappropriate metabolic and immune responses, and damage the barrier function of the intestine, ultimately causing pathology (2–5). This condition typically occurs during intestinal infection by Toxoplasma gondii (T. gondii), a widespread protozoan parasite of animals that also infects humans through the ingestion of oocysts contaminating water or food, or consumption of undercooked meat harboring tissue cysts (6). Host resistance to T. gondii depends on a potent IL-12-dependent IFN-γ response, which is largely mediated by Th1 cells (7–10). However, following oral infection with tissue cysts, the Th1-induced IFN-γ response to T. gondii destroys Paneth cells and blunts their capacity to produce antimicrobial peptides (AMPs), thereby impeding control of invasive Enterobacteriaceae (11–15).
In addition to triggering the IL-12-Th1-IFN-γ axis, oral infection by T. gondii also elicits a Th17 response. How Th17 cells contribute to the host response to T. gondii remains unclear. In two studies, abrogation of IL-17 signaling in Il17ra−/− and Il17a−/− mice resulted in increased susceptibility to T. gondii infection (16, 17). However, another study reported that Il17ra−/− mice as well as B6 mice treated with a blocking anti-IL-17A antibody are more resistant to T. gondii infection (18). Finally, infection with a high dose of T. gondii cysts induced gut immunopathology independent of IL-17, but dependent on IL-22 (19).
We previously found that Th17 response to T. gondii depends on the cell surface molecule class I-restricted T cell-associated molecule (CRTAM) (20). CRTAM was originally described on activated CD8+ T cells and NK cells (21). It binds cell adhesion molecule 1 (CADM1), which is expressed on many myeloid and epithelial cells (22–27). We found that CRTAM is also expressed on intestinal intraepithelial CD4+ T cells upon activation. Moreover, we observed that Crtam−/− and Cadm1−/− mice had a selective defect in Th17 compared to wild-type (WT) mice during infection with the type II T. gondii strain Prugnaud (Pru), which is relatively non-pathogenic. However, they developed an effective Th1 response and cleared gut infection as effectively as WT mice (20). Thus, Th17 deficiency had no obvious consequences in this model of infection.
Here we challenged Crtam−/− mice with an oral inoculation of tissue cysts of the type II ME49 strain of T. gondii, which is more pathogenic than the Pru strain we used previously, despite sharing the same genotype (28). Consistent with our previous study, Crtam−/− mice controlled intestinal infection; they developed an effective Th1 response, but their Th17 response was impaired. Despite being able to control infection, Crtam−/− mice suffered more pathology and many succumbed following infection. Remarkably, certain AMPs that are known to be induced by IL-17 including S100A8, S100A9, and beta defensins, were drastically reduced in the intestines of Crtam−/− mice. As a result, mice lacking CRTAM were unable to control T. gondii-induced dysbiosis and subsequent bacterial translocation to the mesenteric lymph nodes and spleen. Paneth cell-derived AMPs, such as alpha defensins, were not affected, suggesting that control of T. gondii-induced dysbiosis requires a broad spectrum of AMPs. Although IL-17-producing CD4+ T cells were less abundant in Crtam−/− mice than in WT mice, CD4+ T cells expressing RAR related orphan receptor t (Rorγt), the master transcription factor driving Th17, were equally represented; this suggests that CRTAM is required for terminal maturation of Th17 and acquisition of effector function rather than for Th17 lineage commitment. We conclude that CRTAM enables an optimal Th17 host response to pathogenic parasitic infections that is required for controlling dysbiosis and bacterial translocation associated with infection.
T. gondii Infection Causes Marked Intestinal Pathology in Crtam−/− Mice
Given our previous observation that CRTAM has a limited impact on the response to a non-pathogenic strain of T. gondii, we wanted to re-examine CRTAM function in the context of intestinal infection by the more pathogenic ME49 strain. Crtam−/− and WT mice were infected orally with 10 cysts of T. gondii ME49, which is capable of causing death at higher inoculum. This line of ME49 also expresses luciferase and therefore can be visualized by bioluminescence imaging of the whole mouse (29). Crtam−/− mice lost more weight and more of them died following infection (Figures 1A,B), although not statistically significant, Crtam−/− mice show a trend to control parasite replication better than WT (Figure 1C). Histological analysis of the intestine revealed that Crtam−/− mice had more ileal pathology than did WT mice after infection with T. gondii; Crtam−/− mice displayed loss of epithelial integrity, mucosal and submucosal edema, altered villus architecture, and moderate inflammatory infiltrates (Figures 1D,E), the colon was equally affected in WT and Crtam−/− mice (Figure 1E; Figure S1). We conclude that the cause of increased lethality in Crtam−/− mice is exacerbated pathology rather than uncontrolled infection.
Figure 1. Crtam−/− mice are more susceptible to pathology during T. gondii infection. WT and Crtam−/− mice were infected orally with 10 cysts of T. gondii strain ME49, uninfected mice were used as control. (A) Survival (B) weight loss, and (C) parasite burden were analyzed at the indicated time points. (D) Representative sections of ileum stained with hematoxylin and eosin and (E) histological score in naïve mice and at day 8 post-infection. Symbols and bars in (A,B,E) represent means, error bars represent SEM. Symbols in (C) represent single mice. Data are representative of two independent experiments (n = 4–5). Statistical analysis was performed using Student's t-test or Log-rank Mantel-Cox test for survival curves (*p < 0.05; ***p < 0.001).
CRTAM Deficiency Impairs the Th17 Response to T. gondii
We next sought to investigate the mechanisms by which CRTAM counteracts intestinal pathology. No significant differences were observed in the frequencies of CD4+ T cells producing IFN-γ and IL-22 that were present within intraepithelial (IEL) and lamina propria (LPL) lymphocytes isolated from the small intestine of WT and Crtam−/− mice after infection with T. gondii; however, considerably fewer CD4+ T cells producing IL-17 were detected in Crtam−/− mice (Figures 2A–C). Moreover, cytokine profiles of the ilea of WT and Crtam−/− mice after infection with T. gondii confirmed a strong reduction in the abundance of IL-17A and IL-17F mRNAs in Crtam−/− mice, while the amounts of IFN-γ, IL-22, and IL-10 mRNAs were not significantly different between WT and Crtam−/− strains. Together, these results suggest that CD4+ T cells require CRTAM in order to produce IL-17 during T. gondii infection.
Figure 2. CRTAM deficiency results in an impaired IL-17 production by T cells. WT and Crtam−/− mice were infected orally with 10 cysts of T. gondii strain ME49. Frequencies of (A) intraepithelial and (B) lamina propria IFN-γ, IL-17, and IL-22 producing CD4+ T cells were analyzed at days 5 and 8 post-infection. (C) Expression of Ifng, Il17a, Il17f, Il22, and Il10 in the ileum of infected mice analyzed by quantitative PCR at days 5, and 8 post-infection. Bars represent means, error bars represent SEM. Data are representative of two independent experiments (n = 4). Statistical analysis was performed using Student's t-test (*p < 0.05; **p < 0.01; ***p < 0.001).
Lack of CRTAM Curtails the Production of AMPs That Restrain the Microbiota
Given that the dysbiosis incurred during infection with T. gondii has previously been attributed to IFN-γ-induced death of Paneth cells and consequent loss of AMPs that results in an increase of Enterobacteriaceae (11), we assessed expression of various AMPs in the ilea of Crtam−/− and WT mice infected with ME49. Significantly less S100a8, S100a9, Regenerating islet-derived 3 gamma (Reg3g) and Defensin beta 3 (Defb3) mRNA was detected in Crtam−/− mice than in WT mice at day 5 post-T. gondii infection. In contrast, Defensin alpha 1 and alpha 2 were equally expressed (Figure 3A). 16S rRNA sequencing analysis further showed that although naïve WT and Crtam−/− mice had a similar microbiome (Figures 3B–D; Table S1), an increase in Enterobacteriales (Figure 3C; Table S1) in particular γ-proteobacteria (Figure S2) was observed in infected Crtam−/− mice compared to WT (Figure 3B). Moreover, more bacteria were present in mesenteric lymph nodes and spleens from Crtam−/− mice than in those from WT mice at day 8 post-infection (Figures 3E,F), suggesting that this partial defect in AMPs is sufficient to allow breach of the barrier and bacterial translocation, which may contribute to the death of Crtam−/− mice. Remarkably, S100A8, S100A9, and Defb3 are primarily induced by IL-17, whereas defensin alpha 1 and alpha 2 are chiefly produced by Paneth cells. Our results suggest that the Th17 response to pathogenic challenge with T. gondii prevents dysbiosis by inducing AMPs for the containment of commensal bacteria.
Figure 3. Absence of CRTAM expression results in impaired antimicrobial peptide production. WT and Crtam−/− mice were infected orally with 10 cysts of T. gondii strain ME49. (A) Expression of S100a8, S100a9, Defb3, Reg3g, Defa1, and Defa2 mRNA in the ilea of infected mice at days 0, 5, and 8 determined by quantitative PCR. 16S rRNA sequencing of ileal lumen contents at day 0 or 8 days post-infection (D8). Data are mean bacterial changes at the (B) phyla or (C) order level, and (D) principal coordinates analysis on weighted UniFrac distances (n = 4). Bacterial counts present in the (E) mesenteric lymph node and (F) spleen at day 8 post-infection. Bars represent means, error bars represent SEM. Data are representative of two independent experiments (n = 4–8). Statistical analysis was performed using Student's t-test (*p < 0.05; **p < 0.01; ***p < 0.001).
IL-17 Prevents Bacterial Translocation During T. gondii Infection
To validate the importance of IL-17 for the production of AMPs and control of bacterial translocation during T. gondii infection, WT mice were treated with either anti-IL-17A and anti-IL-17F blocking antibodies or an isotype control and infected with 10 cysts of T. gondii ME49 (Figure 4). Mice that received the anti-IL-17 antibodies expressed less AMP mRNA and had more bacterial translocation to the mesenteric lymph nodes, confirming that IL-17 is essential for preventing dysbiosis. Of note, bacteria were not found in the spleens of mice treated with the anti-IL-17 blocking antibodies, suggesting that other cytokines or chemokines may contribute to barrier protection.
Figure 4. IL-17 blockade curbs AMP production and augments bacterial translocation. WT mice were injected intraperitoneally with 350 μg of anti-IL-17A and anti-IL-17F or an isotype control (I.C.) at days −1 and 5 and infected orally with 10 cysts of T. gondii strain ME49. (A) Expression of S100a8, S100a9, Defb3, and Reg3g mRNA in the ilea of infected mice at day 8 determined by quantitative PCR. Bacterial counts present in the (B) mesenteric lymph node and (C) spleen at day 8 post-infection. Bars represent means, error bars represent SEM. Data are representative of two independent experiments (n = 4). Statistical analysis was performed using Student's t-test (*p < 0.05).
CRTAM Engagement Is Required for Terminal Differentiation of Th17
Th17 differentiation is a multi-step process that requires induction of the master Th17 transcription factor Rorγt in naïve CD4+ T cells (30, 31), followed by maturation of Rorγt+CD4+ T cells into IL-17 secreting cells, which depends on exposure to cytokines, such as IL-23 (32). To understand when CRTAM is required during Th17 development, we assessed expression of Rorγt in lamina propria CD4+ T cells during T. gondii infection in WT and Crtam−/− mice. Rorγt+ T cells were present at similar frequencies in WT and Crtam−/− mice (Figures 5A,B), indicating that CRTAM is dispensable for Th17 lineage specification.
Figure 5. Th17 lineage specification is CRTAM-independent. WT and Crtam−/− mice were infected orally with 10 cysts of T. gondii strain ME49. (A) Representative flow cytometry plots showing percent of rorγt of CD4 T cells and foxp3 and rorgt expression on CD4 T cells. (B) frequencies of Rorγt+ T cells and (C) Rorγt+Foxp3+ T cells in the small intestinal lamina propria of infected mice at day 5. Bars represent means, error bars represent SEM. Data are representative of two independent experiments (n = 3). Statistical analysis was performed using Student's t-test.
Since the effector function of Rorγt+CD4+ T cells is controlled by a subset of regulatory T cells that express both Forkhead box p3 (Foxp3) and Rorγt and suppress intestinal inflammation (33, 34), we asked whether lack of CRTAM facilitates the development of Foxp3+Rorγt+ T cells. However, we didn't see an increase in Foxp3+Rorγt+T cells in Crtam−/− mice (Figures 5A,C). These results suggest that CRTAM does not impact modulation of Th17 through Treg induction.
Host control of T. gondii infection has been shown to rely on a strong Th1 response (6, 7). Our study demonstrates that the Th17 response is important for host survival to intestinal infection by a more pathogenic type II strain of T. gondii. By secreting IL-17, Th17 support the production of AMPs, that prevents the dysbiosis and bacterial translocation associated with pathogenic infection. How Th17 response impacts oral infection by T. gondii has been controversial. While some studies have reported a protective role for IL-17 signaling during T. gondii infection (16, 17), other studies have found that IL-17 can either promote or is irrelevant for immunopathology (18, 19). We postulate that these discrepancies may be due to differences in the T. gondii strains used and doses administered in each study, or differences in the microbiota of the mice used. The ability of the various strains used to disrupt the microbiota and the susceptibility of the microbiota to perturbations induced by T. gondii clearly have a great impact; because the microbiota in genetically identical mice varies enormously in different mouse facilities, replicating any of these studies is challenging.
It has been reported that T. gondii-induced dysbiosis depends on the negative impact of IFN-γ produced by CD4+ T cells on Paneth cells and their ability to produce certain AMPs (11). Our study demonstrates that IL-17-induced AMPs contribute to provide a broad umbrella of protection from dysbiosis that is essential during pathogenic T. gondii infection; therefore a reduced Th17 response during infection results in exacerbated dysbiosis and pathology. Indeed, Crtam−/− mice had a very selective reduction in AMPs induced by IL-17, but expressed AMPs released by Paneth cells at normal levels. Given the role of IL-17 in inducing the differentiation and recruitment of neutrophils (35), we envision that Th17 could potentially act by mobilizing a neutrophilic source of AMPs that, together with Paneth cells, provides the broad spectrum of AMPs required for preventing perturbation of microbiota, particularly the expansion of Enterobacteriaceae. The relative impacts of Paneth cell- and neutrophil-derived AMPs in preventing dysbiosis may vary depending on the type and location of the intestinal infection.
Our data support a role for CRTAM in intestinal Th17 responses, whereas Th1 and Th22 responses seem to be relatively CRTAM-independent. This conclusion is consistent with previous studies focusing on the impact of CRTAM on T cell responses in various contexts (24, 36–38), though it does not concur with the originally proposed role for CRTAM in CD4+ T cell production of IL-22 and IFN-γ (39). Our study further advances our understanding of the role of CRTAM in Th17; clearly it is not required for the initial induction of Rorγt in naïve CD4+ T cells, as Crtam−/− and WT mice have similar percentages of intestinal Rorγt+CD4+ T cells. Rather, CRTAM seems to enable IL-17 production by Th17 cells. Since myeloid cells secrete IL-23 in response to changes in the intestinal microbiota (40, 41) and express the CRTAM ligand CADM1 (26), we speculate that CRTAM-CADM1 interactions may sustain the exposure of Rorγt+CD4+ T cells to myeloid cells producing IL-23, thereby promoting their final maturation into IL-17-producing CD4+ T cells.
In conclusion, our study demonstrates that a protective immune response against T. gondii must both effectively control invading pathogens through the Th1-IFN-γ axis and prevent dysbiosis, barrier leakage and microbial translocation through the Th17-AMP axis. Given that oral infection of mice with T. gondii causes intestinal inflammation and ileitis that resemble some aspects of Crohn's disease (42), our findings suggest that unbalanced representation of CD4+ T cell subsets may have a decisive impact in the onset and progression of human inflammatory bowel disease.
Materials and Methods
Crtam−/− and C57BL/6 mice were bred in a pathogen-free facility at Washington University. Age and sex matched animals were used throughout the experiments and were co-housed from birth. All animal experiments were conducted according to U.S.A. Public Health Service Policy of Humane Care and Use of Laboratory Animals. All protocols were approved by the Institutional Animal Care and Use Committee (School of Medicine, Washington University in St. Louis).
T. gondii Infection and Blocking Antibodies
The luciferase-expressing Me49-Luc type II T. gondii strain was provided by Laura Knoll (29). Tissue cysts were obtained from the brains of infected mice. Experimental mice were infected with 10 cysts of T. gondii Me49 orally. Weight was monitored every 48 h for the first 20 days. Parasite burden was analyzed by bioluminescence measurements. Mice were imaged every 48 h for 20 days by i.p. injection of 150 mg of luciferin D (Biosynth AG) per kg of body weight and using a Xenogen IVIS 100 (Caliper Life Sciences). Data was analyzed with the Living Image Software (Caliper Life Sciences) and is expressed in relative light units. Blocking antibodies anti IL-17A (IL-17F) and IgG1 isotype control were purchased from BioxCell. Mice were injected intraperitoneally with 350 μg at day −1 and +5 of T. gondii infection.
Tissue Histology and Histological Score
Small intestines from mice were obtained and the luminal contents were flushed with cold PBS. The ileal portion was fixed in formalin, longitudinally embedded in paraffin. Five micrometer sections were cut and stained with hematoxylin eosin. Tissue slides were deidentified and scored as previously described (43). Briefly, sections were scored on three factors: (1) lymphocytes infiltration (0, no infiltration, 1, some infiltration, 2, massive infiltration in lamina propria, 3, massive infiltration in lamina propria and muscle) (2) edema (0, no edema, 1, edema <50 μm, 2, edema between 50 and 100 μM, 3, edema >100 μm), and (3) ulceration (0, no ulceration, 1, <10% epithelium ulcerated, 2, between 10 and 20% epithelium ulcerated, 3, >20% epithelium ulcerated). Histology was scored in a blinded fashion and scores were totaled for a cumulative score between 0 and 9.
Tissue Isolation, Flow Cytometry, and Cell Sorting
Small intestine intraepithelial lymphocytes (siIEL) and lamina propria lymphocytes (siLPL) were prepared at day 5 and 8 post-infection as described by Lefrançois and Lycke (44). For flow cytometry, the following fluorophore-labeled monoclonal antibody (mAbs) were used: CD3 (145-2C11), CD8 (53–6.7) from BD Biosciences; CD4 (GK1.5) from eBioscience; and CD45 (30F11.1) from Biolegend. For intracellular stainings, cells were stimulated with the Cell Activation cocktail without brefeldin (Biolegend) for 4 h in the presence of Golgi Plug (BD Biosciences). Surface staining was performed, followed by fixation and permeabilization (BD Cytofix cytoperm Plus kit, BD biosciences). The monoclonal antibodies IL-17A (eBio17B7) from eBiosciences, IFN-γ (XMG1.2) and IL-22 from BD Biosciences were used. For rorgT staining, surface staining was performed, followed by fixation with the eBioscience foxp3 transcription factor buffer set (ebiosciences). The monoclonal antibody anti-Rorγt (AFKJS-9) and foxp3 (FJK-16s) from ebiosciences was used. Samples were processed in a FACSCantoII (BD Biosciences) and analyzed with FlowJo Software (Flowjo LLC). Flow cytometric cell sorting was performed using a FACSAria II (BD Biosciences).
Bacterial Counts in Tissue
Mesenteric lymph nodes and spleens from infected mice were aseptically removed and homogenized in PBS in a Magnalyzer (Roche; 5,000 rpm × 30 s). The homogenized organs were plated in Luria Bertani (LB) agar and incubated at 37°C for 48 h. Colonies in the plates were counted and the colony forming units per organ calculated.
Fecal DNA Extraction, Tissue RNA Extraction, and Quantitative PCR
Fecal DNA was extracted using a QIAmp DNA Stool mini kit and RNA was extracted from ileum samples with an RNeasy Mini kit following manufacturer's recommendations (Qiagen). cDNA was synthesized from RNA with Superscript III first-strand synthesis system for RT-PCR (Invitrogen). RNA expression was analyzed by quantitative PCR using Universal SYBR Green PCR Master Mix (Bio-Rad Laboratories) and an ABI7000 (Applied Biosystems). The following oligonucleotides were used: Il17a Fwd 5′-AGAGCTGCCCCTTCACTTTC-3′, Il17a Rev 5′-TGGGGGTTTCTTAGGGGTCA-3′, Il17f Fwd 5′-CTGGAGGATAACACTGTGAGAGT-3′, Il17f Rev 5′-TGCTGAATGGCGACGGAGTTC-3′, Il22 Fwd 5′-GCTCAGCTCCTGTCACATCA-3′, Il22 Rev 5′-AGCTTCTTCTCGCTCAGACG-3′, ifng Fwd 5′-ACAATGAACGCTACACACTGCAT-3′, ifng Rev 5′-TGGCAGTAACAGCCAGAAACA-3′, GADPH Fwd 5′-ACGGCAAATTCAACGGCACAGTCA-3′, GAPDH Rev 5′-TGGGGGCATCGGCAGAAGG-3′, Defa1 Fwd 5′-TCAAGAGGCTGCAAAGGAAGAGAAC-3′, Defa1 Rev 5′-TGGTCTCCATGTTCAGCGACAGC-3′, Defa2 Fwd 5′-CCAGGCTGATCCTATCCAAA-3′, Defa2 Rev 5′-GTCCCATTCATGCGTTCTCT-3′, Reg3g Fwd 5′-AACAGAGGTGGATGGGAGTG-3′, Reg3g Rev 5′-GGCCTTGAATTTGCAGACAT-3′, S100A8 Fwd 5′-GGAAATCACCATGCCCTCTA-3′, S100A8 Rev 5′-ATCACCATCCGAAGGAACTC-3′, S100a9 Fwd 5′-GTCCAGGTCCTCCATGATGT-3′, S100a9 Rev 5′-TCAGACAAATGGTGGAAGCA-3′, defb3 Fwd 5′-GTCTCCACCTGCAGCTTTTAG-3′, defb3 Rev 5′-ACTGCCAATCTGACGAGTGTT-3′. The expression of target mRNA was calculated and normalized to the expression of the house keeping gene GAPDH using the 2(-ΔΔCT) method.
Relative abundance of γ-proteobacteria was analyzed by quantitative PCR using Universal SYBR Green PCR Master Mix (Bio-Rad Laboratories) and an ABI7000 (Applied Biosystems). The following oligonucleotides were used: γ-Proteobacteria 1080gF 5′-TCGTCAGCTCGTGTYGTGA-3′, g-prot g1202R 5′-CGTAAGGGCCATGATG-3′, EUB1114 Fwd 5′-CGGCAACGAGCGCAACCC-3′, EUB1221 Rev 5′-CCATTGTAGCACGTGTGTAGCC-3′. The relative abundance of γ-proteobacteria was calculated to eubacteria using the 2(-ΔΔCT) method.
16S rRNA Sequencing and Analysis
16S rRNA gene sequencing of ileal lumen samples were processed for DNA isolation using the QIAmp DNA Stool mini kit. The V4 region of 16S rRNA gene was PCR-amplified using barcoded primer described previously (45) and sequenced using the Illumina MiSeq Platform (2 × 250–bp paired-end reads). OTU picking was performed using UPARSE (usearch v.8.0.162) (46), and taxonomy was assigned using the uclust method with the Greengenes 13.8 database (QIIME v1.9) (47).
All analyses were performed with Prism 5.0 (GraphPad). Data were analyzed with a non-paired Student's t-test, survival data was analyzed using Log-rank Mantel-Cox test a p < 0.05 was considered significant.
All animal experiments were conducted according to U.S.A. Public Health Service Policy of Humane Care and Use of Laboratory Animals. All protocols were approved by the Institutional Animal Care and Use Committee (School of Medicine, Washington University in St. Louis), based on the Guide for the care and Use of Laboratory Animals. IACUC Protocol Numbers: 20160128 and 20160220.
LC-B designed and performed experiments, analyzed data, and wrote the manuscript. VC, QW, KM, BD, and JC performed experiments and analyzed data. SG, RN, LS, and C-SH designed experiments, analyzed data, and reviewed the manuscript. MC designed experiments and wrote the manuscript.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
We would like to thank M. Cella for valuable discussions. This research was funded by NIH U01 AI095542 and DK103039 awarded to MC, R01AI136515 awarded to C-SH, DK097317 awarded to RN, NIAID AI118426 awarded to LS, and startup funds from Emory University School of Medicine to LC-B.
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.01423/full#supplementary-material
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Keywords: mucosal immunity, T cells, interleukin 17, Toxoplasma gondii, CRTAM
Citation: Cervantes-Barragan L, Cortez VS, Wang Q, McDonald KG, Chai JN, Di Luccia B, Gilfillan S, Hsieh C-S, Newberry RD, Sibley LD and Colonna M (2019) CRTAM Protects Against Intestinal Dysbiosis During Pathogenic Parasitic Infection by Enabling Th17 Maturation. Front. Immunol. 10:1423. doi: 10.3389/fimmu.2019.01423
Received: 05 February 2019; Accepted: 05 June 2019;
Published: 02 July 2019.
Edited by:Marcello Chieppa, National Institute of Gastroenterology S. de Bellis Research Hospital (IRCCS), Italy
Reviewed by:Laura Knoll, University of Wisconsin - Madison, United States
Bellisa Freitas Barbosa, Federal University of Uberlandia, Brazil
Copyright © 2019 Cervantes-Barragan, Cortez, Wang, McDonald, Chai, Di Luccia, Gilfillan, Hsieh, Newberry, Sibley and Colonna. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Luisa Cervantes-Barragan, firstname.lastname@example.org
†Present Address: Luisa Cervantes-Barragan, Department of Microbiology and Immunology, Emory University, Atlanta, GA, United States