Original Research ARTICLE
Bach2 Deficiency Leads to Spontaneous Expansion of IL-4-Producing T Follicular Helper Cells and Autoimmunity
- 1Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Immunology and Microbiology, Faculty of Basic Medicine, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- 2Shanghai Children's Medical Center, Pediatric Translational Medicine Institute, Shanghai Pediatric Congenital Heart Disease Institute, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
The transcription factor Bach2 is a susceptible gene for numerous autoimmune diseases including systemic lupus erythematosus (SLE). Bach2−/− mice can develop a lupus-like autoimmune disease. However, the exact cellular and molecular mechanisms via which Bach2 protects the hosts from developing autoimmunity remains incompletely understood. Here, we report that Bach2 ablation on T cells, but not B cells, resulted in humoral autoimmunity, and this was associated with expansion of T follicular helper (Tfh) cells and abnormal germinal centers. Bach2 was down-regulated in Tfh cells and directly suppressed by the Tfh-defining transcription factor BCL6. Mechanistically, Bach2 directly suppresses the transcription of Cxcr5 and c-Maf, two key regulators of Tfh cell differentiation. Bach2-deficient Tfh cells were skewed toward the IL-4-producing subset, which induced IgG1 and IgE isotype switching of B cells. Heterozygous Bcl6 deficiency reduced the formation of germinal center and autoantibodies, and ameliorated the pathology in Bach2-deficient mice. Our findings identify Bach2 as a crucial negative regulator of Tfh cells at steady state and prove that Bach2 controls autoimmunity in part by restraining accumulation of pathogenic Tfh cells.
The transcription factor Bach2 belongs to the BTB and Cap'n'collar (CNC) gene family and functions within multiple innate and adaptive lineages to control immune responses (1). Genetic polymorphisms within the BACH2 gene locus in human are associated with numerous autoimmune and allergic diseases including asthma (2), vitiligo (3), multiple sclerosis (4), type I diabetes (5), and systemic lupus erythematosus (SLE) (6). Bach2−/− mice developed autoantibodies and spontaneous lethal inflammatory diseases (7), suggesting an essential role of Bach2 in controlling autoimmunity. Bach2 is highly expressed in GC B cells and promotes antibody class switching and suppresses plasma cell differentiation (8, 9). Bach2 is also expressed by T cells, and directs T helper (Th) cell differentiation, homeostasis, and effector functions (1). Bach2 constrains full effector differentiation within Th1, Th2, and Th17 cells in vitro (7). Bach2 maintains Th cells in a naïve state by suppressing effector memory-related genes (10). Bach2 promotes Foxp3+ regulatory T (Treg) cell differentiation and enhances Treg-mediated immunity (7, 11). More recently, we reported that SUMO-specific protease 3 (SENP3) act as a pivotal regulator of Treg cells by controlling the SUMOylation and nuclear localization of Bach2 (12). In addition, Bach2 suppresses the differentiation of Th2 cells, and Bach2-deficient mice develop lethal lung-specific Th2-type allergic inflammation (13, 14). These findings help explain the emergence of Bach2 as a key suppressor of autoimmunity.
T follicular helper (Tfh) cells, a unique subset of CD4 helper T cells, are specialized in providing cognate help to B cells to form GCs. Within GCs, Tfh cells interact with B cells through the co-stimulatory and co-inhibitory molecules, including CD40L, ICOS, and programmed cell death protein 1 (PD-1), to facilitate the selection and maturation of high-affinity B cells (15, 16). However, excessive Tfh cells impair positive selection by reducing competition among B cells for T cell help, thus yielding a lower threshold for selection that allows for emergence of self-reactive clones. Aberrant expansion of Tfh cells results in pathogenic autoantibodies and is frequently associated with autoimmune diseases, including SLE (17–19). Numerous mouse studies have supported a causal role of Tfh cells in autoimmune diseases (20–22). In addition, increased blood memory Tfh cells were frequently observed in human autoimmune diseases such as SLE, and positively correlated with serum autoantibody titers and disease severity (23, 24), suggesting an important role of Tfh cells in the pathogenesis of these diseases. Tfh cells are proposed to be potential therapeutic targets in human autoimmune diseases.
Tfh cells are generally considered to be of a separate Th cell lineage and arise from naive CD4+ T cells with sequential steps in response to T-cell-dependent antigen (15, 16). Naive CD4+ T cells are primed by dendritic cells and up-regulates the chemokine (C-X-C motif) receptor CXCR5, which enables them to migrate into B cell follicles to further differentiate into Tfh cells. However, Tfh cells can also be generated from the conversion of other effector T cells under the conditions of chronic and sustained antigenic stimulation (25, 26). Tfh cell differentiation is tightly controlled by numerous lineage-specific transcription factors including B cell lymphoma 6 (BCL6), c-Maf, and Blimp1 (27, 28). BCL6 is a master transcription factor of Tfh cells, and Tfh cell differentiation is completely abrogated in Bcl6-deficient CD4+ T cells (29–31). c-Maf acts as a positive regulator of Tfh cell differentiation in mice and humans, and induces the expression of many Tfh-related genes including Cxcr5 and IL-21 (32–34). Interestingly, some regulators of Tfh cell differentiation, such as transcription factor Ets1 (35), are encoded by human autoimmunity-prone genes, and their deficiency causes Tfh-driven humoral autoimmune diseases in mice.
In this study, we report that genetic deletion of Bach2 in T cells, but not in B cells, recapitulated the lupus-like autoimmunity in Bach2−/− mice. Bach2 deficiency in T cells results in spontaneous accumulation of IL-4-producing Tfh cells. We also provide the evidence that Bach2 controls autoimmunity in part by restraining aberrant Tfh cell formation.
Materials and Methods
Bach2fl/fl mice (ES Clone ID: EPD0689_1_H02) were generated at the Wellcome Trust Sanger Institute and the neo cassette was removed after crossing with FLPe transgenic mice (The Jackson Laboratory). Cd4-Cre, Cd19-Cre, and ERT2-Cre transgenic mice were from The Jackson Laboratory. Bcl6fl/fl mice are described previously (36). All mice were crossed on a C57BL/6J background. All mice were maintained in a specific pathogen-free (SPF) facility, and all animal experiments were in accordance with protocols approved by the Institutional Animal care and Use Committee (IACUC) of Shanghai Jiao Tong University, School of Medicine.
Flow Cytometry and Antibodies
Single-cell suspensions of spleens, MLNs, and PPs were prepared from fresh tissues by standard procedures and surface-stained in FACS buffer with monoclonal antibodies. The following antibodies were obtained from eBioscience, BD PharMingen, or BioLegend: anti-B220-PE, FITC, BV650 or APC (RA3-6B2), anti-Fas PE-Cy7 (Jo2), anti-CD38-PE or PerCP/Cy5.5, anti-GL7-FITC (GL7), anti-IgM-APC (II/41), anti-IgG1-FITC (RMG1-1), anti-IgE-FITC (RME-1), anti-CD86-APC (GL1), anti-ICOS-FITC (7E.17G9), anti-CD44-APC (1M7), anti-CD62L-FITC (MEL-14), anti-PD1-PE or FITC (J43), and anti-CD4-APC or APC-CY7 (GK1.5). CXCR5 was stained with biotinylated anti-CXCR5 (clone 2G8) and streptavidin-conjugated PE, APC, or PE/CY7 (BD Biosciences). For intracellular staining, cells were fixed and permeabilized with “Foxp3 staining buffer set” (eBioscience) following the manufacturer's protocol and then stained with anti-Foxp3-PE (FJK-16s, ebiosciences) and anti-BCL6-PerCP/CY5.5. To identify cytokine secretion, lymphocytes were routinely prepared and stimulated with PMA (phorbol 12-myristate 13-acetate, 20 ng/ml, Invitrogen) and inomycin (1 μg/ml, Invitrogen) in the presence of Golgi-Plug (1 μg/ml, Invitrogen) for 5 h. Cells were stained with anti-CD4 antibodies, followed by permeabilization in Fix/Perm buffer (BD, Pharmingen), and intracellular staining in Perm/Wash buffer (BD Pharmingen) with anti-IL-4-PE (11b11) and anti-IFN-gamma-FITC (XMG1.2). NP-PE was from Biosearch Technologies. FACS analysis was performed on a Fortessa II (BD), and data were analyzed with FlowJo software (Tree Star).
Kidneys were fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 7 μm, and stained with hematoxylin and eosin. Immunohistochemistry was performed as described previously (9). Briefly, after antigen retrieval and pretreatment with 3% hydrogen peroxide, tissue sections (7 μm) were then incubated with biotin-conjugated PNA (Vector Laboratories) overnight at 4°C, followed by streptavidin–alkaline phosphatase and detected by the Alkaline Phosphatase Substrate Kit III (Vector Laboratories). The sections were then boiled for 10 min, incubated with biotin-conjugated anti-B220 (RA3-6B2, Caltag metsystems), followed by streptavidin-HRP and developed using DAB kit. To visualize IgG position in the glomeruli, kidney sections (7 μm) were incubated with blocking buffer and stained with HRP-conjugated anti-mouse IgG antibody (Jackson ImmunoResearch), followed by streptavidin-HRP and developed using DAB kit. Images were taken using a color camera (AxioCam; Carl Zeiss Microimaging) and were analyzed using Axiovision software (Carl Zeiss Microimaging).
Immunization and Tamoxifen Treatment
Mice were immunized intraperitoneally (i.p.) with SRBCs (1 × 108 cells per mouse). Tamoxifen (80 mg/kg, Sigma) in corn oil (Sigma) was given by oral gavage on day 8 post immunization to induce the activity of the Cre recombinase. Mice were analyzed on day 5 after tamoxifen treatment.
Mixed Bone Marrow Chimera
To determine the intrinsic role of Bach2, a mixture of congenic CD45.1+ wild-type bone marrow cells (80%) together with CD45.2+ tested bone marrow cells (20%) either from WT or Bach2ΔCD4 mice were transferred intravenously into lethally irradiated (5.5 Gy, twice) CD45.1+ WT recipient mice. After at least 8 weeks of bone marrow reconstitution, the recipients were subjected for analysis.
RNA Sequencing and Data Analysis
A total of 1–3 × 104 Tfh cells (CD4+B220−CD44+PD-1+CXCR5+) were sorted from the MLNs of WT and Bach2fl/flCd4-cre mice using a FACSAria II cell sorter (BD Biosciences) at the core facility of Shanghai Institute of Immunology. cDNA was synthesized and amplified using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). The library for mRNA sequencing was generated using Illumnia TruSeq Preparation Kit (RS-122-2001) and sequenced using Illumnia Nextseq500 sequencer. The mapping rate was 96% overall across all the samples in the dataset. HTSeq was used to quantify the gene expression counts from Tophat2 alignment files. Differential expression analysis was performed on the count data using R package DESeq2. P values obtained from multiple tests were adjusted using Benjamini-Hochberg correction. Significantly differentially expressed genes are defined by a Benjamini-Hochberg corrected P value < 0.01 and fold change > 2.
Enzyme-Linked Immunosorbent Assay (ELISA) and Detection of Autoantibodies
Serum titers of immunoglobulin subclasses were determined by specific ELISA kits (SouthernBiotech) according to the manufacturer's protocol. To detect anti-dsDNA autoantibodies in sera, high-binding ELISA plates were coated overnight with 2 μg/ml dsDNA from calf thymus (Sigma-Aldrich). Coated plates were blocked with 1% BSA and 0.5% gelatin in TBS for 2 h at room temperature, and diluted samples were incubated overnight at 4°C in TBS with 1% BSA. Bound anti-dsDNA antibodies were detected with AP-conjugated anti-mouse IgG (Jackson ImmunoResearch) and streptavidin-HRP (Bioresearch) followed by TMB substrate solution (eBioscience). Absorbance was measured at 450 nm. Serum titers of anti-ANA antibodies were determined by ANA Hep Screen ELISA kit (Demeditec) according to the manufacturer's protocol.
Naive CD4+ T Cell Isolation and Differentiation in vitro
Naive CD4+ T (CD44lowCD62LhighCD25−) cells were purified using a CD4+ naive T cell negative isolation kit according to the manufacturer's protocol (STEMCELL Technologies). The in vitro differentiation experiments were performed as previously described. Naive CD4+ T cells were stimulated with immobilized anti-CD3 (5 μg/ml; 145-2C11; eBioscience) and anti-CD28 (5 μg/ml; 37.51; eBioscience) for 2 days. Then, the cells were washed and transferred to a new plate and further expanded in medium with hIL-2 (50 U/ml, R&D Systems) for 2 days. For Tfh-like cell differentiation, naive CD4+ T cells were activated with anti-CD3 and anti-CD28 as above and treated with 20 ng/ml IL-6 (R&D Systems), 20 ng/ml IL-21 (R&D Systems), 10 μg/ml anti-IL-4 (11B11, eBioscience), 10 μg/ml anti-IFN-γ (XMG1.2, eBioscience), and 20 μg/ml anti-TGF-β (1D11, R&D Systems) for 4 days.
Total RNA was prepared with Trizol (invitrogen) and cDNA was synthesized using Superscript reverse transcriptase and random primers (Invitrogen). Quantitative PCR (qPCR) was performed using Power SYBR Green PCR master mix (Vazyme). The sequences of gene-specific primers are listed in the Supplementary Information. All reactions were performed in triplicate and results were calculated by the change-in-threshold (2−ΔΔCT) method with Actβ as housekeeping reference gene.
Spleen naive CD4+ T cells were stimulated with immobilized anti-CD3 (5 μg/ml; 145-2C11; eBioscience) and anti-CD28 (5 μg/ml; 37.51; eBioscience) antibodies for 2 days before analysis. Human Tfh cells were isolated from tonsils as described previously (37). Cells were cross-linked with 1% formaldehyde and neutralized with 0.125 M glycine. Cell lysates were sonicated to 300–500 bp and immunoprecipitations were performed with anti-BCL6 (N-3; Santa Cruz Biotechnology Inc.), anti-BACH2 (9), or IgG as a control. After complete washing, immunoprecipitated DNA was eluted in elution buffer and reversely cross-linked at 65°C overnight. DNA was purified and quantified by real-time PCR. Gene-specific primers are listed in Table S1.
Student's t-test was performed for statistical analysis. The software GraphPad Prism 5 was used for this analysis. P values higher than 0.05 are considered to be not significant.
Bach2 Ablation in T Cells Is Sufficient to Recapitulate the Autoimmunity in Bach2−/− Mice
Bach2−/− mice have been known to develop autoantibodies and age-related lethal autoimmunity (7). To determine which types of immune cells play key roles in disease development, we generated cell-type-specific Bach2-deficient mice where Bach2 was deleted in CD4+ T cells (Bach2ΔCD4) or B cells (Bach2ΔCD19). Bach2ΔCD4 mice were undistinguishable from the Cd4-Cre mice (designated WT) littermates by 2 months of age. From 3 months of age, Bach2ΔCD4 mice spontaneously developed a progressive wasting disease that resulted in diminished survival compared to WT mice (Figures 1A,B). Two- to 3-month-old Bach2ΔCD4 mice generated higher concentrations of antibodies to double-stranded DNA (anti-dsDNA) and nuclear antigen (anti-ANA) in their serum (Figure 1C), which are the hallmarks of autoimmune diseases in humans. In contrast, these abnormalities were not detected in Bach2ΔCD19 mice (Figures 1A–C). Two- to 3-month-old Bach2ΔCD4 mice contained significantly higher amounts of serum IgM, IgG1, and IgA compared to age-matched WT controls (Figure 1D). However, the titers of serum IgG2b, IgG2c, and IgG3 were comparable between the two groups (Figure 1D). We also detected higher titers of anti-dsDNA and anti-ANA IgG1, but not other IgG2 subclasses, in the serum of these Bach2ΔCD4 mice (Figure 1E and data not shown). Aged Bach2ΔCD4 mice displayed prominent IgG deposits in kidney glomeruli (Figure 1F), and spontaneously developed splenomegaly and lymphadenopathy (Figure 1G). These abnormalities became prominent when they reached 5–6 months of age and showed signs of illness. In summary, these results demonstrate that Bach2 in T cells is essential to prevent humoral autoimmunity in mice.
Figure 1. Loss of Bach2 in T cells causes humoral autoimmunity. (A,B) Body weight (A) and survival rate (B) of wild-type (WT), Bach2ΔCD4, or Bach2ΔCD19 mice at 5 months of age. (C) Titer of anti-dsDNA antibodies and anti-nuclear antibodies (ANA) in the sera of WT, Bach2ΔCD4, or Bach2ΔCD19 mice at 2–3 months of age. (D) Titers of immunoglobulins in sera of WT and Bach2ΔCD4 mice at 2–3 months of age. (E) Titers of anti-dsDNA and ANA IgG1 in the sera of WT and Bach2ΔCD4 mice at 2–3 months of age. (F) Immunochemistry of IgG deposit in renal glomeruli. (G) Representative images (left) of spleen and lymph nodes of WT and Bach2ΔCD4 mice at 5 months of age. The weight of spleens from indicated mice is shown at the right. Each symbol represents one mouse; small horizontal lines indicate the mean; ns, not significant; *P < 0.05 and **P < 0.01 (two-tailed t-test).
Bach2 Deficiency Results in Excessive Tfh Cells and Aberrant GC B Cells in Mesenteric Lymph Nodes and Peyer's Patches
Abnormal Tfh cell responses can lead to the development of autoimmunity (19, 26). We next examined whether Tfh cells and GCs were altered in Bach2ΔCD4 mice. GCs are chronically induced in the mesenteric lymph nodes (MLNs) and Peyer's patches, as a result of continuous B cell stimulation by a wide range of microbiota and food-derived antigens. For the next analyses, we used 8–10-week-old Bach2ΔCD4 mice before they developed lymphoproliferative disease. The MLNs from Bach2ΔCD4 mice had normal cellularity, but a lower proportion of CD4+ T cells when compared to the WT counterparts (Figure S1A). Consistent with previous reports, Bach2ΔCD4 mice contained a higher frequency of CD44hi active/memory fraction in the CD4+ compartment within the MLNs (Figure S1B). However, Bach2ΔCD4 mice had a significantly higher frequency and absolute number of follicular T cells (CXCR5+PD-1+ or CXCR5+Bcl6+) among CD4+ T cells than WT mice (Figure 2A). We next sought to determine whether expansion of follicular T cells in Bach2ΔCD4 mice was selective to Tfh (CXCR5+PD-1+Foxp3−) and/or Tfr (CXCR5+PD-1+Foxp3+) cells. We found that among CXCR5+PD-1+ follicular T cell compartments in Bach2ΔCD4 mice, the ratio of Tfh to Tfr cells was shifted significantly toward the Tfh cell subset (Figure 2B), suggesting an expansion of Tfh cells to a larger extent than that of Tfr cells. Ablation of Bach2 in T cells has been shown to decrease Foxp3+ Treg cells (7). Indeed, the percentage of Treg cells tended to decrease in the MLNs of Bach2ΔCD4 mice, although with no significance (Figure S1C). The absolute number of Treg cells in the MLNs of Bach2ΔCD4 mice was significantly reduced because of their decreased CD4+ T cell compartment (Figure S1C). Interestingly, we found that the frequencies of Tfr cells among Foxp3+ CD4+ Treg cells and Tfh cells among Foxp3− CD4+ T cells increased by about two- and four-fold in the MLNs of Bach2ΔCD4 mice compared to WT mice, respectively (Figure S1D). The absolute numbers, however, were significantly elevated only for Tfh but not Tfr cells in Bach2ΔCD4 mice (Figure S1D). Thus, the Tfr/Tfh ratio, a critical factor dictating the magnitude of antibody production, was reduced in Bach2ΔCD4 mice due to greater expansion of Tfh cells relative to Tfr cells.
Figure 2. Bach2ΔCD4 mice display excessive Tfh cells and aberrant GC B cells in the MLNs. Lymphocytes from the MLNs of WT and Bach2ΔCD4 mice at 2–3 months of age were subjected for analysis. (A) Representative flow cytometry plots, frequency quantification, and absolute number of CXCR5+PD-1+ and CXCR5+Bcl6+ T follicular cells (gated on CD4+B220− cells). (B) Representative flow cytometry plots and frequency quantification of Tfh (Foxp3−) and Tfr (Foxp3+) cells among CXCR5+PD-1+ CD4 T cells. (C) Representative flow cytometry plots, frequency quantification, and absolute number of CD38lo/−Fas+ GC B cells among splenic live B220+ cells. Quantification of the ratio of CD86hiCXCR4lo light zone (LZ) to CD86loCXCR4hi dark zone (DZ) B cells among GC B cells (bottom). (D) Representative flow cytometry plots and frequency quantification of IgG1+ and IgE+ cells among splenic GC B cells. All data were from at least two independent experiments. Each symbol represents one mouse, and small horizontal lines indicate the mean. ns, not significant; *P < 0.05 and **P < 0.01 (two-tailed t-test).
We then investigated whether excessive Tfh cells induced abnormal GCs in the MLNs of Bach2ΔCD4 mice. Bach2ΔCD4 mice had a significantly higher frequency and absolute number of Fas+CD38lo GC B cells in the MLNs when compared to WT mice (Figure 2C). Moreover, Bach2ΔCD4 mice had a greater proportion of CXCR4loCD86hi light zone cells among GC B cells (Figure 2C). The proportions of IgG1+ and IgE+ GC B cells were significantly increased in the MLNs of Bach2ΔCD4 mice (Figure 2D), indicating that Bach2 in Tfh cells affects antibody isotype switch within B cells. Similarly, excessive Tfh cells and deregulated GCs were observed in the Peyer's patches of Bach2ΔCD4 mice (Figure S2). To further determine the cell-intrinsic role of Bach2 in constraining Tfh cells, we generated mixed bone marrow chimeras by reconstituting lethally irradiated CD45.1+ WT mice with a 4:1 ratio mixture of bone marrow cells from CD45.2+ Bach2-deficient and WT donor mice, respectively (Figure S3). Eight weeks after transplantation, we observed that CD45.2+ Bach2-deficient CD4+ T cells developed more Tfh cells compared to CD45.2+ WT CD4+ T cells in the MLNs and Peyer's patches (Figure S3), indicating that Bach2 regulates Tfh cell formation intrinsically. In conclusion, Bach2 in T cells is critical to limit Tfh cell differentiation and regulate the GC responses in chronic GCs.
Bach2 Deficiency in T Cells Enhances the Formation of Spontaneous GCs in the Spleen
Spontaneous GCs are induced without immunization or infection, and contribute to steady-state antibody production. Aberrant spontaneous GCs produce pathogenic autoantibodies and have been described in many autoimmune diseases such as SLE (21). We next explored whether Bach2 deficiency in T cells affects the generation of spontaneous GCs. We found that 4–5-month-old Bach2ΔCD4 mice had significantly higher frequencies of CXCR5+PD-1+ follicular T cells in their spleens (Figure 3A). Among the follicular T cell compartment in Bach2ΔCD4 mice, the Tfh to Tfr cell ratio shifted significantly toward the Tfh cell subset in their spleens (Figure 3A). Furthermore, the frequency of GC B cells was significantly elevated in the spleens of Bach2ΔCD4 mice compared to WT mice (Figure 3B). Immunohistochemical staining further revealed that the spleens of Bach2ΔCD4 mice contained considerably more and larger peanut agglutinin (PNA)-positive GCs than did their WT counterparts (Figure 3C). Although the frequencies of dark zone B cells among GC B cells in the spleen of Bach2ΔCD4 mice were equivalent to that of WT mice (Figure 3B), the proportion of IgG1+ and IgE+ cells was significantly higher (Figure 3D). Interestingly, the accumulation of Tfh cells and GC B cells was not observed in the spleens of young Bach2ΔCD4 mice at 6–8 weeks of age (data not shown). In conclusion, Bach2 in T cells is required to limit the formation of spontaneous GCs under steady state.
Figure 3. Bach2ΔCD4 mice develop spontaneous GCs in the spleens with age. The lymphocytes from the spleens of WT and Bach2ΔCD4 mice at 3–5 months of age were subjected for analysis. (A) Representative flow cytometry plots and frequency quantification of splenic CXCR5+PD-1+ T follicular cells (gated on CD4+B220− cells) (top). Representative flow cytometry plots and frequency quantification of Tfh (Foxp3−) and Tfr (Foxp3+) cells among CXCR5+PD-1+ CD4 T cells (bottom). (B) Representative flow cytometry plots and frequency quantification of GC B cells among splenic live B220+ cells. Quantification of the ratio of LZ to DZ B cells among GC B cells (bottom). (C) Representative PNA staining of splenic sections from WT and Bach2ΔCD4 mice (scale bars, 1 mm). Blue, PNA; brown, B220. (D) Frequencies of IgM+, IgG1+, and IgE+ cells among GC B cells. All data were from at least two independent experiments. Each symbol represents one mouse, and small horizontal lines indicate the mean. ns, not significant; *P < 0.05 and **P < 0.01 (two-tailed t-test).
Bach2 Is Down-Regulated During Tfh Cell Differentiation and Directly Suppressed by BCL6
A previous study showed that naive CD4+ T cells express higher levels of Bach2 compared to effector/memory CD4+ cells (30). To better understand the role of Bach2 in Tfh cells, we first examined the mRNA abundance of Bach2 during Tfh cell differentiation. Quantitative RT-PCR revealed that Bach2 mRNA abundance was markedly reduced during Tfh cell differentiation (Figure 4A). Then, we sought to figure out the mechanism by which Bach2 is suppressed in Tfh cells. The transcriptional repressor BCL6 is highly expressed in Tfh cells and acts as a master regulator of Tfh differentiation (15–17). Therefore, we hypothesized that Bach2 may be a direct target of BCL6. By analyzing published chromatin immunoprecipitation sequencing (ChIP-Seq) of human primary tonsillar Tfh cells (35), we identified multiple BCL6 binding peaks at the BACH2 gene promoter and gene body (Figure 4B). The binding of BCL6 at the BACH2 promoter region was confirmed by ChIP followed by quantitative PCR (Figure 4C). To investigate whether BCL6 represses Bach2 expression, WT and Bcl6ERT2Cre mice were immunized i.p. with SRBCs, a T-cell-dependent antigen, to induce Tfh cell response in the spleen. On day 8 after immunization, mice were administrated with one dose of tamoxifen by oral gavage to induce Cre activity for Bcl6 deletion in Tfh cells. Acute deletion of Bcl6 in Tfh cells led to an increase of Bach2 mRNA abundance (Figure 4D). Collectively, these results demonstrate that BCL6 directly binds to Bach2 and suppresses its transcription.
Figure 4. Bach2 is down-regulated during Tfh cell differentiation and directly suppressed by BCL6. (A) Relative Bach2 mRNA abundance in naïve (CD44lowCD62Lhigh), non-Tfh (CD44highCD62LlowCXCR5−PD1−), and Tfh cells isolated from the MLNs and/or spleens of WT mice at 2 months of age, determined by RT-qPCR. (B) The illustration depicting ChIP-seq tracks of BCL6 and indicated histone marks at the BACH2 gene in primary human Tfh cells. BCL6 binding peaks are shown at the top. (C) ChIP-qPCR analysis of BCL6 binding peak indicated by arrow in (B). The fold enrichment was calculated relative to IgG. Data are mean ± s.e.m. (D) The relative mRNA expression levels of Bach2 in Tfh cells sorted from the MLNs and spleens of 2-month-old WT and Bcl6ERT2Cre mice 5 days post tamoxifen treatment. Data are mean ± s.e.m. of two mice per group. *P < 0.05 and **P < 0.01 (two-tailed t-test).
Bach2 Represses Tfh-Related Genes Including C-Maf and Cxcr5
To gain further insight into the mechanisms by which Bach2 inhibits Tfh cell differentiation, we compared the mRNA abundance of a group of key molecules known to be crucial for the differentiation of Tfh cells in WT and Bach2-deficient naive CD4+ T cells activated in vitro under unbiased conditions (Th0) or conditions that promote the differentiation of Tfh cell-like (Tfh-like) cells. Bach2 deficiency promotes activated CD4+ T cells to express high level of IL-4 (Figure 5A), in agreement with a previous report (13, 14). Interestingly, the mRNA levels of Cxcr5 and c-Maf were up-regulated by about 6- and 32-fold in Bach2-deficient vs. WT CD4+ T cells cultured in Th0 cell condition, respectively (Figure 5A). Moreover, the mRNA levels of Cxcr5 and c-Maf were increased by about seven-fold in Bach2-deficient CD4+ T cells cultured under Tfh-like cell conditions (Figure 5A). However, the expression levels of other Tfh cell-related genes, including Bcl6, Batf, and Icos, were largely unchanged, suggesting that Bach2 may suppress Tfh differentiation mainly via repression of Cxcr5 and c-Maf. In line with this, Bach2-deficient non-Tfh (CD4+CD44highCXCR5−PD1−) cells expressed higher mRNA levels of c-Maf, Cxcr5, and IL-4, but not Bcl6 (Figure 5B). Unexpectedly, Bach2 appeared not to affect the expression of c-Maf and Cxcr5 in Tfh cells (Figure 5B), and this is likely due to low abundance of Bach2 in these cells (Figure 4A). Finally, we observed that Bach2-deficient naïve CD4+ T cells contained a greater proportion of CXCR5+ cells and expressed higher c-Maf protein abundance in vivo (Figure 5C). The protein expression of CXCR5 and c-Maf was comparable between WT and Bach2-deficient Tfh cells (Figure 5D).
Figure 5. Bach2 negatively regulates the mRNA abundance of Cxcr5, Maf, and IL-4. All cells were isolated from 2-month-old mice for analysis. (A) Relative mRNA levels of indicated genes in splenic naïve CD4+ cells (CD44lowCD62Lhigh) cultured in Th0 or Tfh-like cell condition. Data were from two independent experiments and represented as fold changes relative to WT cells after normalization with Actb. Numbers adjacent to black bars indicate fold in each. (B) RT-qPCR analysis of mRNA levels of indicated genes in WT and Bach2-deficient non-Tfh (CD44highCXCR5−PD1−) and Tfh cells. Data were represented as up-regulation relative to WT non-Tfh cells after normalization with Actb. (C) The frequency of CXCR5+ cells and c-Maf protein abundance in naïve CD4+ T cells from the MLNs. (D) Flow cytometric analysis of CXCR5 and c-Maf protein abundance in Tfh cells. (E) The illustration of ChIP-seq tracks of Bach2 at indicated genes in CD4+ T cells (left). Bach2-bound sites were indicated by black lines. ChIP-qPCR analysis of Bach2 binding at indicated genomic loci. The fold enrichment was calculated relative to IgG. Data are mean ± s.e.m. of two independent experiments from at least three mice per group. **P < 0.01 (two-tailed t-test).
We next investigated the molecular mechanism by which Bach2 suppresses Cxcr5 and c-Maf transcription. Bach2 is known to act primarily as a transcription repressor. By analyzing published Bach2 ChIP-Seq data in activated CD4+ T cells (13), we found that Bach2 was recruited to the intron region of IL-4, the promoter region of c-Maf and upstream of the promoter of Cxcr5 (Figure 5E). The binding of Bach2 at these loci were further confirmed by ChIP-qPCR in activated CD4+ T cells (Figure 5E), indicating that Bach2 negatively and directly regulates IL-4, c-Maf, and Cxcr5. Interestingly, Bach2 bound to the IL-4 gene, but not Cxcr5 and c-Maf genes in Tfh-like cells (Figure S4), consistent with the observation that WT and Bach2-deficient Tfh cells expressed the similar levels of Cxcr5 and c-Maf (Figures 5B,D).
Bach2 Deficiency Promotes Tfh Cells to Skew Toward IL-4-Producing Subset
To further elucidate the molecular mechanisms by which Bach2 regulates Tfh cell differentiation in vivo, we performed RNA sequencing to compare the transcriptome between WT and Bach2-deficient Tfh cells. The global gene expression between WT and Bach2-deficient Tfh cells were highly concordant (Pearson r2 = 0.92, three replicates for each group), and key molecules known to be critical for Tfh cell differentiation, including Cxcr5 and c-Maf, had similar expression in the two groups of Tfh cells. Then, we selected genes from published datasets (GEO accession codes GSE21379) that are up-regulated and down-regulated in Tfh cells relative to their expression in non-Tfh cells (38), for gene set enrichment analysis (GSEA) with our data. This analysis revealed that Bach2-deficient Tfh cells did not show significant enrichment for the genes up-regulated or down-regulated in the Tfh lineage (Figure 6A), further suggesting that Bach2-deficient Tfh cells are “true” Tfh cells. Only 356 genes were significantly (padj < 0.01; change in expression ≥2-fold) up-regulated and 298 genes were down-regulated in Bach2-deficient Tfh cells (Figure 6B). Among them, we found that Bach2-deficienct Tfh cells expressed higher IL-4, but lower IFN-γ, IL17-f, and Foxp3 at mRNA levels (Figure 6B). Moreover, the Th2 master regulator Gata3 was also increased in Bach2-deficienct Tfh cells (Figure 6B). Flow cytometry further revealed that Bach2-deficient Tfh cells contained a higher proportion of IL-4-secreting cells, but a lower frequency of IFN-γ-secreting cells, in both the MLNs and spleens (Figures 6C,D), indicating that Bach2 deficiency promotes Tfh cells to skew toward IL-4- producing subset.
Figure 6. Bach2-deficient Tfh cells are skewed IL-4-producing subset. Two- to 3-month-old mice were used for analysis. (A) GSEA analysis of gene signatures up-regulated or down-regulated in Tfh cells relative to their expression in non-Tfh cells, from published data (GEO accession code: GSE21379). (B) Scatterplot of genes up-regulated (red) or down-regulated (blue) in Bach2-deficient Tfh cells vs. WT cells. Select genes of interest are labeled. (C,D) Representative flow cytometry plots and quantification of IL-4 and IFN-γ-secreting Tfh subsets (gated in CXCR5+PD-1+ T follicular cells) in the MLNs (C) and spleens (D). Data are mean ± s.e.m. of two independent experiments from at least three mice per group. *P < 0.05 and **P < 0.01 (two-tailed t-test).
Heterozygous Bcl6 Deficiency Reduces Autoantibodies and Attenuates Pathology in Bach2ΔCD4 Mice
Autoantibodies result from a breakdown of tolerance mechanisms during B cell development, from T-cell-dependent or -independent B cell activation, or as a consequence of aberrant GC reactions. BACH2 has been shown to regulate many aspects of T cell differentiation and effector functions (1). Therefore, it is important to understand whether excessive Tfh cells account for the generation of autoantibodies and autoimmunity in Bach2ΔCD4 mice. To this end, we crossed mice carrying LoxP-flanked Bcl6 alleles with Bach2ΔCD4 mice to generate Bcl6fl/+Bach2ΔCD4 mice. The Bcl6 heterozygosity was capable to limit the spontaneous and induced GC responses (20). As expected, the Bcl6 heterozygosity significantly reduced the frequencies of Tfh cells and chronic GC B cells in the MLNs of Bach2ΔCD4 mice at 5 months of age (Figures 7A,B). The expansion of spontaneous GC B cells in the spleens of these mice was also markedly attenuated, although the reduction of Tfh cells was not significant (Figures 7A,B). We then tested whether reduction in the chronic and spontaneous GC responses in Bcl6fl/+Bach2ΔCD4 mice was accompanied by reduced pathology. We found that the Bcl6 heterozygosity significantly increased the survival rate and prevented weight loss in Bach2ΔCD4 mice at 5–6 months of age (Figure 7C). The titers of autoantibodies are often parallel with the disease severity and activity in various autoimmune diseases (23, 24). Morbid Bach2ΔCD4 mice contained significantly higher titers of serum anti-dsDNA and anti-ANA antibodies when compared with the normal counterparts (Figure 7D). Although anti-dsDNA and anti-ANA antibodies were easily detected in the sera of Bcl6fl/+Bach2ΔCD4 mice, their titers were significantly lower than those in morbid Bach2ΔCD4 mice (Figure 7D).
Figure 7. The Bcl6 heterozygosity significantly reduced autoantibodies and pathology. (A,B) The frequencies of Tfh and GC B cells in the MLNs and spleens from Bach2ΔCD4 and Bcl6fl/+Bach2ΔCD4 mice. (C) Body weight and survival of Bach2ΔCD4 and Bcl6fl/+Bach2ΔCD4 mice. (D) Titers of anti-dsDNA and anti-ANA antibodies in sera from Bach2ΔCD4 and Bcl6fl/+Bach2ΔCD4 mice. All mice were analyzed at 5–6 months of age. Each symbol represents one mouse; small horizontal lines indicate the mean. ns, not significant; *P < 0.05 and **P < 0.01 (two-tailed t-test).
Our study gains new insights into Bach2-dependent control of humoral autoimmunity. Through our cell-type-specific Bach2-deletion experiments, we confirm that Bach2 expression in T cells, but not in B cells, is essential for prevention of humoral autoimmunity. Bach2 restrains accumulation of Tfh cells, especially IL-4-producing subset, under chronic antigenic stimulation and steady state. Furthermore, we provide evidences to show that Bach2 prevents humoral autoimmunity, at least in part by inhibiting the generation of pathogenic Tfh cells. More recently, two groups reported that Bach2 limits Tfh cell differentiation in response to acute stimulation of foreign antigens (39, 40). In combination with our results, Bach2 appears to be an important negative regulator during Tfh cell differentiation under various conditions. In addition to Tfh cells, Bach2 instructs the differentiation and effector functions of other T cell subsets including Treg and Th17 cells. Deregulated Treg and Th17 cells play prominent roles in autoimmunity. We observed partial loss of Treg cells in Bach2ΔCD4 mice. It is likely that these mice also harbor pathogenic Th17 cells. These abnormalities may significantly contribute to autoimmunity in Bach2ΔCD4 mice.
CXCR5 is a lineage-defining marker of Tfh cells, and Tfh cell differentiation is initiated by CXCR5 up-regulation (15, 16). Bach2 was reported to directly suppress Cxcr5 transcription (39). This conclusion was further confirmed in our study. In addition, we found that Bach2 is capable to directly repress c-Maf, a key regulator of Tfh cells. c-Maf is up-regulated during Tfh cell differentiation and facilitates the expression of many Tfh-related genes including Cxcr5, Icos, Pdcd-1, and IL-21 (32–34). Loss of c-Maf expression in the T cell compartment leads to defective development of Tfh cells in response to both antigen/adjuvant vaccinations and commensal intestinal bacteria (34). Thus, it is conceivable here that Bach2 deficiency results in aberrant Tfh cell formation mainly via up-regulation of c-Maf and CXCR5. Unexpectedly, although ectopic expression of Bach2 was shown to repress Bcl6 expression in differentiated Tfh cells induced by acute immunization with protein antigen (40), Bach2 deletion had no significant effect on Bcl6 mRNA abundance in Tfh cells induced by chronic antigenic stimulation. The different results may be due to different experimental settings.
Bach2 is highly expressed in GC B cells and is required for antibody class switching and blockade of plasma cell differentiation (8, 9). In contrast, Bach2 is gradually down-regulated during Tfh cell differentiation and maintained at low levels in differentiated Tfh cells (Figure 4A). Currently, little is known about the transcriptional control of Bach2 in T cells. BCL6 may be a crucial transcriptional factor that binds to the Bach2 gene locus and suppresses its transcription (Figures 4B,C). The generation and function of Tfh cells are regulated at multiple stages. Bach2 is likely to act mainly at the early phase to limit aberrant Tfh formation. This is evidenced by the observation that Bach2 directly suppresses c-Maf and Cxcr5 levels in non-Tfh cells, but not in Tfh cells. Low Bach2 levels may be required for Tfh cells to maintain their identity, supported by the recent finding that ectopic overexpression of Bach2 in murine Tfh cells led to a rapid loss of their phenotype and subsequent breakdown of the GC response (40). Thus, the dynamic expression of Bach2 in B cells and T cells could be crucial in instructing proper germinal center responses.
Tfh cells are heterogeneous cell populations, and some Tfh cells are capable of expressing Th1- or Th2-signature cytokines, IFN-γ or IL-4, both of which contribute to the regulation of B cell antibody class switching within GCs. However, the mechanisms regulating IFN-γ/IL-4-producing subset distribution in Tfh cells remain elusive. Bach2-deficient Tfh cells are skewed toward the IL-4-secreting subset, subsequently resulting in a greater proportion of IgG1+ and IgE+ B cells. To our knowledge, Bach2 is the first reported factor that regulates the balance of Tfh subsets. As the GC response evolves, differentiated Tfh cells extinguish IL-21 secretion and switch to IL-4 production (41). The switching may be repressed by Bach2 because Bach2 inhibits IL-4 expression in Tfh cells. Mechanistically, Bach2 directly binds to the regulatory region of IL-4 to inhibit its transcription. In addition, Bach2 may indirectly suppress IL-4 transcription via down-regulation of Gata3. IL-4-secreting Tfh cells can originate from Th2 cells in response to chronic antigens such as helminth (42). It is possible that Bach2 specifically represses the conversion of Th2 into IL-4-producing Tfh cells in the lymphoid tissues in the presence of continuous antigenic stimulations. Further experiments are needed to test this hypothesis.
Genetic polymorphisms in the BACH2 gene locus are associated with numerous autoimmune and allergic diseases in human. Our genetics study in mice suggests that humans carrying the genetic variations in the BACH2 gene may develop autoimmune diseases by inducing excessive pathogenic Tfh cells. Targeting Tfh cells may represent an effective therapeutic strategy for these patients.
The RNA-seq data reported in this paper have been deposited in the NCBI Gene Expression Omnibus under accession number GEO135087.
HZ and QH conducted most of the experiments and analyzed the data. MZ and ZZ conducted RNA-seq analysis. FY and CP performed immunochemistry and ELISA. CH conceived the idea for the project, supervised the study, and wrote the manuscript.
This work was supported in part by the National Key R&D Program of China (2018YFC0115900) to CH, the 1000 Youth Elite Program, the National Natural Science Foundation of China (31870872) to CH, and Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine to HZ.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.02050/full#supplementary-material
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Keywords: Bach2, T follicular helper cells, IL-4, autoimmunity, BCL6
Citation: Zhang H, Hu Q, Zhang M, Yang F, Peng C, Zhang Z and Huang C (2019) Bach2 Deficiency Leads to Spontaneous Expansion of IL-4-Producing T Follicular Helper Cells and Autoimmunity. Front. Immunol. 10:2050. doi: 10.3389/fimmu.2019.02050
Received: 20 May 2019; Accepted: 14 August 2019;
Published: 04 September 2019.
Edited by:Yun-Cai Liu, Tsinghua University, China
Reviewed by:Xindong Liu, Army Medical University, China
Youn Soo Choi, Seoul National University College of Medicine, South Korea
Copyright © 2019 Zhang, Hu, Zhang, Yang, Peng, Zhang and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Chuanxin Huang, email@example.com
†These authors have contributed equally to this work