Abstract
Chronic lymphocytic leukemia (CLL) is a malignancy of mature, antigen-experienced B lymphocytes. Despite great progress recently achieved in the management of CLL, the disease remains incurable, underscoring the need for further investigation into the underlying pathophysiology. Microenvironmental crosstalk has an established role in CLL pathogenesis and progression. Indeed, the malignant CLL cells are strongly dependent on interactions with other immune and non-immune cell populations that shape a highly orchestrated network, the tumor microenvironment (TME). The composition of the TME, as well as the bidirectional interactions between the malignant clone and the microenvironmental elements have been linked to disease heterogeneity. Mounting evidence implicates T cells present in the TME in the natural history of the CLL as well as in the establishment of certain CLL hallmarks e.g. tumor evasion and immune suppression. CLL is characterized by restrictions in the T cell receptor gene repertoire, T cell oligoclonal expansions, as well as shared T cell receptor clonotypes amongst patients, strongly alluding to selection by restricted antigenic elements of as yet undisclosed identity. Further, the T cells in CLL exhibit a distinctive phenotype with features of “exhaustion” likely as a result of chronic antigenic stimulation. This might be relevant to the fact that, despite increased numbers of oligoclonal T cells in the periphery, these cells are incapable of mounting effective anti-tumor immune responses, a feature perhaps also linked with the elevated numbers of T regulatory subpopulations. Alterations of T cell gene expression profile are associated with defects in both the cytoskeleton and immune synapse formation, and are generally induced by direct contact with the malignant clone. That said, these abnormalities appear to be reversible, which is why therapies targeting the T cell compartment represent a reasonable therapeutic option in CLL. Indeed, novel strategies, including CAR T cell immunotherapy, immune checkpoint blockade and immunomodulation, have come to the spotlight in an attempt to restore the functionality of T cells and enhance targeted cytotoxic activity against the malignant clone.
Introduction
Chronic lymphocytic leukemia (CLL) is an age-related malignancy characterized by the accumulation of monoclonal mature B cells expressing CD5, CD19 and CD23 on their surface (1). Numerous studies support that the clonotypic B cell receptor immunoglobulin (BcR IG) is critically implicated in disease pathophysiology (2). Indeed, the intensity of intracellular signaling downstream of the BcR in CLL cells is associated with cell proliferation and disease severity (3), whereas restrictions in the gene repertoire of the clonotypic BcR IG strongly highlight the role of antigenic triggering in disease pathogenesis (4, 5).
Different lines of evidence suggest that interactions with two main categories of microenvironmental components i.e. soluble factors and other extracellular elements as well as bystander cells are implicated in shaping a supportive tumor microenvironment (TME) in CLL (6–8). The former category includes various extracellular molecules (e.g.) cytokines, chemokines, antigens but also, in a broader sense, products of metabolic processes. Indeed, although cellular metabolism in CLL remains largely unexplored, increasing evidence implicates oxidative stress in the natural history of CLL by showing e.g. that the accumulation of reactive oxygen species (ROS) may hold prognostic significance (9, 10).
Turning to bystander cells, different subpopulations of T cells as well as natural killer (NK) cells accumulate in vivo along with mesenchymal stromal cells (MSC) and nurse-like cells (NLCs), forming a complex network that favors clonal expansion and proliferation of the malignant clone (11–13). Ongoing crosstalk of CLL malignant cells with these other cell populations in the TME affects the function of both parties. On the one hand, this leads to immunosuppression, a hallmark of CLL associated with increased susceptibility to infections, autoimmune manifestations, and a higher incidence of secondary malignancies (14). On the other hand, external triggers support the survival and proliferation of the neoplastic cells (15); this was first made evident when it was found that CLL cells undergo apoptosis in suspension cultures, which can be partially rescued by co-cultures with stromal cells or NLC (11).
T cells are major contributors to adaptive immunity, actively engaged in defense against pathogens and tumor cells through a great variety of accessory and effector functions. Upon encounter with a specific antigen, T cells are activated and eventually differentiate into various distinct subpopulations, acquiring either cytotoxic or helper properties. Pathogen clearance, mediated by cytotoxic T cells or through the activation of other cell types induced by cytokines secreted from T helper cells, is followed by the apoptosis of the effector T cells as a homeostatic mechanism that restores the immune system at the pre-activation state. Simultaneously, a small fraction of antigen-specific memory T cells are resting in the body, ready to generate an immediate and effective secondary response (16, 17). This homeostatic balance is perturbed in CLL, where, similar to various solid or hematological malignancies, T cells exhibit a number of phenotypic and functional defects undermining their normal immune responses (18). Moreover, T cells appear to have an active involvement in CLL development and evolution, as supported by experimental evidence that the transfer of autologous activated T cells in NOD/Shi-scid, γcnull (NSG) mice is a prerequisite for successful engraftment of CLL cells in murine models (19, 20). Interestingly, the post-transfer outgrowth of functionally competent Th1 T cells seen in NSG mice highlights the suppressive and inhibitory TME in CLL patients, particularly considering reports that these T cells can regain their functionality and promote B cell diversification and differentiation (18). It has been proposed that this phenomenon may reflect selection for Th1 cells in vivo, release from various inhibitory mechanisms operating within the human host or a special effect of the distinct NSG microenvironment. Arguably, similar mechanisms may also act in CLL patients, however, strong correlations remain elusive (20), highlighting the need for caution when attempting extrapolations based on findings deriving from model systems.
In CLL, as in other cancers, genomic instability leads to various alterations that serve as a source of cognate antigens, sufficient for the induction of tumor-reactive T cell responses (21–24). Although tumor-/antigen-specific T cells have been identified in CLL patients, these are incapable of effectively eliminating neoplastic cells due to several intertwined immunosuppressive mechanisms that establish T cell dysfunction (25, 26), thus, favoring escape from immune surveillance (27). Evidence suggests that this tolerogenic TME is actively induced by the CLL cells themselves through the secretion of cytokines and chemokines that affect cellular functions in the bystander cells (15). As a matter of fact, a recent study demonstrated that shared peptides from the highly conserved BcR IG of patients belonging to CLL stereotyped subsets 1 and 2 could generate antigen-specific T cells upon presentation in HLA-restricted manner. Furthermore, that study reported T cell specific reactivity against CLL cells, while also showing that immunization of Eμ-TCL1 mice with BcR IG-derived peptides led to anti-leukemic T cell responses, implying that targeted T cell immunotherapy against the clonotypic BcR IG represents an appealing treatment option (28). Along similar lines, the identification of the unique set of neo-epitopes of each patient could lead to the isolation of leukemia-specific T cells, that can be used as a novel treatment approach (29).
Taken together, deciphering the cross-talk mechanisms between CLL cells and bystander T cells as well as the implicated signaling pathways is reasonably anticipated to offer insights into disease pathophysiology, and, potentially, a great opportunity for designing microenvironment-directed treatment approaches.
T Cell Subpopulation Imbalances in CLL
Alterations in the T cell composition have been extensively described in CLL, however the exact impact of these changes on disease development and progression remains controversial. The first described T cell abnormality in a CLL-specific context concerned the finding of increased numbers of T cells in the periphery, accounting mostly for CD8+ T cell expansion that leads to inversion of the normal CD4+/CD8+ cell ratio (30). Of note, these CD8+ T cells are severely compromised as to their capacity to exert cytotoxicity (31). Regarding the potential clinical relevance of these findings, it has been reported that elevated numbers of CD8+ cells are associated with disease progression as well as shorter time to first treatment and progression-free survival, perhaps partly due to co-expression of the inhibitory receptor of programmed death-1 (PD-1) (32). However, under different conditions oligoclonal expansion of CD8+ effector cells in the Eµ-TCL1 mouse model of CLL has been associated with disease control, whereas conversely, ablation of CD4+ T cells did not affect disease progression (33).
Expansions of different CD4+ T cell subpopulations that exert either pro-tumoral activity or immunosuppression have been reported in CLL. Early studies have shown that IFN-γ secreting Th1 cells can provide trophic signal for CLL cells inhibiting programmed cell death and supporting survival (34). On the other hand, T cell-mediated immune responses have been linked with disease control in cases of autologous CLL regression, highlighting the multi-dimensional T cell activity into the CLL TME (35). Further, expansion of a novel CD4+ T subpopulation characterized by the expression of the TIGIT (T cell immunoreceptor with Ig and ITIM domains) inhibitory receptor has been recently observed in patients at advanced stages of CLL; these cells were shown to support CLL survival in in vitro experiments (36). Finally, CD4+PD-1+HLA-DR+ T cells that co-express inhibitory and activation markers have been associated with aggressive disease (37). Altogether, these apparently conflicting findings clearly indicate the need for delving deeper into the distinct subsets and functions of the T cell compartment in CLL.
A well-characterized finding in CLL concerns the elevated numbers of T regulatory cells (Tregs) (30, 38) that are generally known to contribute to cancer progression through dampened antitumor responses and immunosuppression (39, 40). Of note, CLL Tregs are more suppressive than normal Tregs, whereas depletion of these cells led to efficient anti-tumor responses in animal models of CLL (41, 42). Additionally, interleukin 4 (IL-4) secreted from Tregs also induces anti-apoptotic pathways in CLL cells through the overexpression of the anti-apoptotic protein BCL2, which is therapeutically targeted by venetoclax, an effective agent for the treatment of CLL (43–46). Hence, efforts to inhibit Tregs through targeting the FoxP3 transcription factor, that is critical for their function (47, 48), or other interacting molecules in the downstream pathway could be clinically useful, at least in principle, similar to what has been proposed for other cancers (49, 50).
Th17 cells represent another T cell subpopulation with a critical role in immune homeostasis, actively participating in inflammatory processes. Imbalances in Th17 populations have been linked with autoimmune disorders, however their role in cancer remains to be fully elucidated (51). In CLL, Th17 cells are increased compared to healthy individuals; however, increased Th17 cells in cases at early disease stages have been reported to be associated with a favorable clinical outcome, conceivably through controlling the expansion of Tregs (52, 53). Altogether, the aforementioned evidence suggests that the balance between Tregs and Th17 cells may impact on the clinical outcome through as yet unknown mechanisms (14, 51).
Functional Impairment of T Cells in CLL: Causes, Functional Consequences, and Clinical Implications
T cell activation starts when an antigen-presenting cell (APC) introduces an epitope bound on an HLA molecule to a specific TR. A second signal from the CD28 receptor, constitutively expressed on T cells, enhances cell proliferation, generation of cytotoxic lymphocytes, and cytokine production (54). In CLL, in vitro T cell activation through CD28 followed by infusion of the activated autologous T cells to the patients was shown to lead to competent anti-leukemic effects and decreased TR clonality. Based on this evidence, ex vivo T cell activation and expansion was proposed as a valid treatment option in CLL, however failed to gain ground (55).
Effective activation of naïve T cells through normal interactions with APCs requires the formation of the immune synapse, a specialized contact area between T cells and APCs (56). Impaired immune synapse formation has been documented as a hallmark of CLL and shown to be mechanistically linked to alterations in the expression of genes associated with actin polymerization, cytoskeletal organization and vesicle trafficking (57). Of note, immune synapse defects appear to be contact-dependent, as shown in both ex vivo studies of primary patient samples and in vivo studies of animal models of CLL (58, 59).
Such effects may dampen effective antitumor responses, through a complex network of inhibitory signals and immunosuppressive interactions amongst CLL cells and T cells into TME. Importantly, signals delivered through the CTLA-4 and PD-1 receptors modulate T cell activation status through reduction of TR signaling and cytolytic functions. These inhibitory molecules are overexpressed in T cells of patients with CLL, while their ligands are also overexpressed by the malignant cells, hence putting the brakes on anti-tumor activity (60). Specifically, CLL cells express inhibitory surface molecules from B7 and TNF-receptor families, namely CD200, CD274 (PD-L1), CD276 and CD270 which are implicated in synapse formation defects in both autologous and allogeneic T cells, suggesting a novel evasion mechanism (61).
On these grounds, research into these receptors and ligands could pave the way to novel treatment modalities for CLL, whereby T cell-driven immune responses could be re-invigorated through targeting the interactions between inhibitory receptors and their ligands. However, despite encouraging pre-clinical results, the clinical application of antibodies against these receptors (immune checkpoint inhibitors [ICI]) has been frustratingly disappointing in CLL, with only minimal effectiveness when administered as monotherapy (62, 63). Evidently, more research is warranted in order to define the precise role of ICIs in the management of CLL and also better understand the mechanisms underlying the emergence of severe adverse events reported for these agents (64, 65).
In CLL, immune synapse defects were found to be reversible by the administration of lenalidomide, an immunomodulatory drug (IMiD) that appears to reshape the expression of cytokines and orchestrate cell-mediated responses, mobilizing the T cell compartment (66, 67). In addition, lenalidomide treatment invigorates T cell motility and migration through activation of integrin lymphocyte function–associated antigen-1 (LFA-1), also affected by direct contact with CLL cells (68). Relevant to mention, clinical studies of lenalidomide as monotherapy or consolidation therapy after chemoimmunotherapy have reported restored immune synapse formation and downstream signaling and well as improved quality of clinical responses (69, 70).
T cells in CLL display features of exhaustion, a functional state initially described in the setting of chronic infections but later also recognized in various cancer types (25, 71–75). In such conditions, exhaustion has been postulated to arise as a result of persistent antigenic stimulation, leading to gradual loss of T cell effector functions (76–78). Similar to other contexts, exhausted T cells in CLL are characterized by low proliferative rates, reduced cytotoxicity, altered cytokine production and, as already mentioned, expression of multiple inhibitory receptors on their surfaces, including CTLA-4, PD-1 and LAG-3 (25, 79, 80).
The functional analogies between T cells in individuals exposed to persistent antigenic stimulation due to a chronic infection and individuals affected by CLL allows arguing that antigenic pressure is key to shaping the T cell repertoire and functionality also in CLL. That notwithstanding, the exact nature of the cognate antigens/epitopes implicated in T cell selection in CLL is yet to be defined. However, the most prominent (classes of) candidates concern: (i) the same antigens that are implicated in the selection of the CLL progenitors or the malignant cells themselves; (ii) tumor-derived epitopes from aberrant proteins expressed by the malignant cells e.g. due to a pathogenic mutation or other genomic aberration; and (iii) the clonotypic IG, perhaps the most abundant clone-specific molecule (81–84) (Figure 1).
Figure 1
Antigen Selection Shapes the T Cell Compartment in CLL: Immunogenetic Evidence
The clonotypic BcR IG is critically implicated in the natural history of CLL, a fact amply supported by the therapeutic efficacy of agents interfering with BcR IG signaling, which have changed dramatically the therapeutic landscape of CLL (85–87). This clinical evidence complements immunogenetic evidence that the BcR IG is a key driver in disease ontogeny and evolution. In more detail, the BcR IG gene repertoire is characterized by remarkable restrictions (88), culminating in BcR IG stereotypy where, at clear odds with serendipity, different clones share (quasi)identical IG (4, 5, 89). Moreover, the molecular characteristics of the clonotypic BcR IG have established prognostic and predictive significance since patients with a significant imprint of somatic hypermutation (SHM) display indolent disease, in contrast to those with few or no SHM who generally follow a more aggressive clinical course (90, 91). Taken together, these features emphasize the links between BcR IG structure and clonal behavior, while also highlighting the importance of antigen selection in CLL pathophysiology (92, 93).
Considering the importance of extracellular cues for CLL cell survival and proliferation, as well the T cell compartment defects in CLL patients, the interest in the TR gene repertoire of T cells in CLL and the implicated antigens is hardly surprising. Immunophenotypic studies in the 1990s described T cell clonal expansions in CLL patients, prompting speculations that these cells could be possibly related to the host immune response against CLL-derived antigens, or emerged as a result of a longitudinal interplay between CLL and T cells in the proliferative centers (94). Moreover, analyses of the TR beta (TRB) chain gene rearrangements by Southern blotting offered the first molecular evidence for skewed TRBV gene repertoire and oligoclonality in both CD4+ and CD8+ T cells of CLL patients compared to healthy aged-matched controls (95–97). Additionally, stimulation of isolated T cells from CLL patients with autologous malignant cells resulted in monoclonal TR expansions, prompting speculations that CLL patients could bear a pool of T cells that specifically recognize leukemia-associated antigens (95, 98). Similar findings of decreased clonal diversity were also reported in Eμ-TCL1 mice supporting the notion of CLL-dependent antigen-driven selection pressure on the TR gene repertoire (99).
These initial studies were followed by significantly more extended examinations of the TR gene repertoire by our group, first through subcloning of TRBV-TRBD-TRBJ gene rearrangements followed by Sanger sequencing (100) and, more recently, by high-throughput approaches using next generation sequencing (NGS) (101, 102). Summarizing our findings: (i) the TRBV gene repertoire is skewed; (ii) oligoclonal T cell expansions feature prominently, particularly amongst cytotoxic T cells; (iii) such expansions persist and may even increase during the disease course; and, (iv) identical or highly similar TR clonotypes were shared between different patients, especially those belonging to the same stereotyped subset, and these appeared to be CLL-biased as they were not present in other contexts. Altogether, these findings allow arguing that antigen selection has a key role in shaping the TR repertoire in CLL, while also favoring the intriguing hypothesis that the relevant antigens are most likely CLL-related (101). However, the exact mechanism that underlies T cell clonality and retains these expanded populations during the disease course remains elusive.
In order to further understand T cell compartment dynamics in CLL, we extended our studies to longitudinal investigation (pre/post-treatment) of patients treated with chemoimmunotherapy with the fludarabine-cyclophosphamide-rituximab (FCR) regimen or the BcR signaling inhibitors (BcRi) ibrutinib (IB) and idelalisib (the latter in combination with rituximab, R-ID). We found that T-cell clonality significantly increased at (i) 3 months in the FCR and R-ID treatment groups, and (ii) over deepening clinical response in the R-ID group, with a similar trend detected in the IB group. Perhaps more importantly, in contrast to FCR that induced T-cell repertoire reconstitution, BcRi retained pretreatment clones. Extensive comparisons of the CLL dataset against external TR sequence databases showed little similarity with other entities, but instead revealed major TR clonotypes shared exclusively by patients with CLL, supporting selection by conserved CLL-associated antigens. In addition, we assessed the functional impact of these treatments on T cells and found that (i) R-ID upregulated the expression of activation markers in effector memory T cells, and (ii) both BcRi improved antitumor T-cell immune synapse formation, in marked contrast to FCR (102). Here, it is worth mentioning the contradictory findings of an another NGS-based longitudinal study of the TR gene repertoire in IB-treated patients, where this therapy resulted in TR repertoire diversification (103). However, major differences in the experimental and analytical procedures render these two studies incomparable, highlighting the need for further harmonization of the NGS protocols and bioinformatics workflows. A limitation inherent to both studies concerns the fact that total CD3+ T cells rather than particular T cell subpopulations were investigated, which could lead to misinterpretation due to a “dilution effect” by bulk T cell analysis. Finally, robust conclusions regarding the links, if any, between T cell dynamics overtime and clinical outcome are still not possible: hence studies on larger, well-characterized cohorts remain of paramount importance.
Considering mounting evidence that the TR gene repertoire in CLL is antigen selected, identifying the cognate antigens is an obvious line of research, not least because it would assist in designing effective antigen-specific immunotherapy. Studies of HLA-presented antigenome and computational models for tumor-associated antigen prediction proposed the existence of disease specific antigens, deriving from CLL-related genomic aberrations, that were identified exclusively on neoplastic cells of CLL patients (104–106). Additionally, the broad representation of BcR IG-derived epitopes in patients across different disease (Binet) stages and through various treatments suggest them as potential candidates for peptide vaccines and objectives of neoantigen-targeting immunotherapies. However, immune tolerance mechanisms can turn the BcR IG-derived peptides, a potentially significant pool of cognate neo-antigens in B cell lymphomas (107, 108), into ineffective targets for T cells. This phenomenon, known as immunoediting, has been described in different contexts (including cancer and viral infections) and can neutralize an immunogenic phenotype favoring cells to survive and proliferate (27, 109).
Treatment Options in CLL and Implication of the T Cell Compartment
T cells in the CLL TME exhibit a number of phenotypic and functional defects, acquired through persistent crosstalk with the neoplastic cells. The T cell compartment aberrations appear to be CLL-specific and induced by the CLL cells, leading to functional exhaustion, reduced activation and proliferation of effector subpopulations and decreased cytotoxic responses. Thus, CLL cells have the ability to amend alter T cell functions in order to evade immune-surveillance, proliferate and further expand overtime.
Despite the severe T cell deficiencies identified in CLL patients and models, studies demonstrate that subsets of these defective T cells can restore their functionality and mount anti-tumor responses under particular therapeutic interventions (Figure 2). Moreover, the identification of tumor-derived antigens implicated in T cell clonal expansions that may harbor anti-tumor properties can pave the way for therapeutic peptide vaccination strategies and adoptive T-cell transfer protocols in order to augment efficacy and guide the specificities of anticancer immune responses. Consequently, deep understanding of tumor-derived T cell dysfunction is highly significant for the development of new therapeutic modalities to restore anti-tumor immunity and result in tumor control.
Figure 2
In recent years, the advent of BCRi has led to a major paradigm change in the treatment of CLL. Due to structural and functional similarities of kinases between T and B cells, cross-reactivity of these agents has been described, highlighting an additional immunomodulatory mechanism of action in TME. In more detail, besides inhibiting the Bruton’s tyrosine kinase (BTK) that is implicated in signal transduction of the activated BcR in CLL cells (110), ibrutinib targets also the T-cell associated kinase ITK, modulating T cell immunity. This is made manifest by effective immune synapse formation, shift towards Th1 polarization, and expansion of functionally competent specific-T clones, possibly contributing in deepening clinical response (102, 111, 112). In addition, ibrutinib-induced down-regulation of the PD-1/PD-L1 axis and inhibition of the STAT3 pathway modulates the immunosuppressive TME: on these grounds, combination protocols with lenalidomide or checkpoint inhibitors hold promises for reactivation of anti-tumor immunity (113–115). Similar effects have been demonstrated by treatment with idelalisib that also targets other PI3K isoforms in T cells, enhancing anti-tumor functions through modulation of Tregs (74). This is unsurprising considering that intracellular signaling that leads to suppression of T cell effector functions is extensively dependent on pathways implicating an isoform of PI3Kδ that constitutes an excellent target for idelalisib. At a first glance, this effect might be considered as an alternative mechanism to reduce immunosuppression and invigorate T cell responses; however, the described severe autoimmune adverse effects cannot be overlooked (116). Effects on the CLL TME are not restricted to BCRi though, since treatment with the BCL2 inhibitor venetoclax has been associated with reduced numbers of PD-1+ T cells and expression of inflammatory cytokines, suggesting a mechanism of immune recovery (117). Collectively, thorough investigation of the possible immunomodulatory effects of novel agents in CLL is of great relevance for future development of new combination therapeutic strategies.
As already mentioned, interactions between inhibitory receptors and their ligands, including the PD-1/PD-L1 axis, enable CLL cells to evade T cell immunosurveillance, thus proposing a new area for therapeutic targeting. Preclinical studies in a murine CLL model showed that immune checkpoint inhibition of PD-1/PD-L1 led to prevention of immune dysfunction and leukemia development, hence offering a rationale for the clinical testing of these regiments (58). Despite the promising results of PD-1 blockade in clinical trials for metastatic melanoma (MM), non-small cell lung cancer (NSCLC) and renal cell cancer (RCC) (118), anti–PD-1 monotherapy benefits only a small fraction of CLL patients who developed Richter syndrome (64). Nevertheless, combination therapies with regimens targeting different inhibitory pathways may offer better outcomes (71, 119).
Adoptive T cell therapy is an evolving field that shows promises in recent trials. The protocol is based on the infusion of genetically modified T lymphocytes with a particular specificity for antigen recognition on the malignant cells. In CLL, the expression of the CD19 surface molecule is used as a selective B cell target and autologous cytotoxic T cells are modified ex vivo to express a chimeric antigen receptor (CAR) with affinity to CD19 of CLL cells. The engagement of a CAR-T cell with a CD19+ B cell leads to T cell activation in a MHC-independent manner, redirecting cytotoxic effects towards CLL cells (120). Encouraging results from adoptive T cell therapy in B acute lymphoblastic leukemia have paved the way for trials in CLL: initial results were deemed positive, but the overall efficiency of the approach was limited in patients with bulky lymph node involvement (121). Attempts to induce the anti-tumor properties and the efficiency of the CAR-T cells are based on, amongst others, modifications for constitutive expression of the CD40 ligand that lead to proliferation and Th1 immune responses, whereas also combination with PD-1 blockade has been used in order to bypass CAR-T exhaustion (121, 122).
A major challenge for effective CAR-T cell designs is to enhance and guide specificity towards the malignant cells. Thus, identification and in-depth characterization of T cell clones bearing anti-tumor properties emerge as highly relevant. In parallel, detailed studies on the HLA-lingadome highlight tumor-derived antigens that are exclusively expressed on the leukemic cells and represent possible effective targets for adoptive T cell therapy (107, 123). Additionally, the identification of anti-CLL T clones and the respective disease-specific cognate antigens offers a rationale for peptide vaccination strategies in order to induce anti-tumor responses, but also for target recognition for T-cell based immunotherapy. Indeed, spectrometric studies on the naturally presented HLA ligands in CLL identified a pool of antigens exclusively expressed in CLL patients, providing information regarding the immunogenicity of the malignant cells while also offering new treatment targets (107). However, clinical studies targeting neoepitopes expressed in the malignant cells demonstrate enhanced rejection of tumor cells, underling the safety profile of these approaches (124, 125).
Concluding Remarks
CLL cells have the ability to transform the effector functions of the bystander T cells in the TME, thus rendering them a source of trophic signals for the survival and proliferation of the malignant clone. Moreover, alterations in the T cell transcriptome combined with functional exhaustion undermine cytotoxic responses against tumor cells, establishing tumor evasion and escape of CLL cells from immunosurveillance. That said, despite the observed defects in the T cell compartment, several studies have identified subsets of T cells bearing anti-tumor properties that can be unleashed under the proper stimulation. This binary, contradicting role of T lymphocytes in CLL TME supports the analogy with a two-edged sword. Based on the observations discussed in the current review, the mobilization of exhausted T cells and the invigoration of anti-tumor pathways could be translated to clinical efficiency, tumor control and durable remissions. However, in order for this objective to be met, detailed characterization of the implicated mechanisms at the molecular and cellular level is urgently warranted.
Funding
This work is supported in part by the ERANET Transcan-2, Project Acronym: Novel, and the Hellenic Precision Medicine Network in Oncology.
Statements
Author contributions
All authors contributed to the article and approved the submitted version. EV wrote the manuscript. KS and AC edited the text and gave final approval.
Acknowledgments
The authors wish to acknowledge the work of past and current group members, particularly Drs Anna Vardi, Fotis Psomopoulos, Kostas Pasentsis, and Niki Stavroyianni.
Conflict of interest
KS and AC have received unrestricted grant support from Jannsen Pharmaceutica and Abbvie.
The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
KippsTJStevensonFKWuCJCroceCMPackhamGWierdaWGet al. Chronic lymphocytic leukaemia. Nat Rev Dis Prim (2017) 3:1–22. doi: 10.1038/nrdp.2016.96
2
BurgerJAChiorazziN. B cell receptor signaling in chronic lymphocytic leukemia. Trends Immunol (2013) 34:592–601. doi: 10.1016/j.it.2013.07.002
3
ChiorazziNStevensonFK. Celebrating 20 Years of IGHV Mutation Analysis in CLL. HemaSphere (2020) 4:e334. doi: 10.1097/hs9.0000000000000334
4
AgathangelidisAChatzidimitriouAGemenetziKGiudicelliVKarypidouMPlevovaKet al. Higher-order connections between stereotyped subsets: Implications for improved patient classification in CLL. Blood (2020). doi: 10.1182/blood.2020007039
5
StamatopoulosKBelessiCMorenoCBoudjograhMGuidaGSmilevskaTet al. Over 20% of patients with chronic lymphocytic leukemia carry stereotyped receptors: Pathogenetic implications and clinical correlations. Blood (2007) 109:259–70. doi: 10.1182/blood-2006-03-012948
6
ShainKHDaltonWSTaoJ. The tumor microenvironment shapes hallmarks of mature B-cell malignancies. Oncogene (2015) 34:4673–82. doi: 10.1038/onc.2014.403
7
GhiaPChiorazziNStamatopoulosK. Microenvironmental influences in chronic lymphocytic leukaemia: The role of antigen stimulation. J Internal Med (J Intern Med) (2008) 549–62. doi: 10.1111/j.1365-2796.2008.02030.x
8
HerishanuYKatzBZLipskyAWiestnerA. Biology of Chronic Lymphocytic Leukemia in Different Microenvironments. Clinical and Therapeutic Implications. Hematol Oncol Clin North Am (2013) 27:173–206. doi: 10.1016/j.hoc.2013.01.002
9
D’ArenaGVitaleCPerbelliniOCosciaMLa RoccaFRuggieriVet al. Prognostic relevance of oxidative stress measurement in chronic lymphocytic leukaemia. Eur J Haematol (2017) 99:306–14. doi: 10.1111/ejh.12918
10
D’ArenaGSenecaEMigliaccioIDe FeoVGiudiceALa RoccaFet al. Oxidative stress in chronic lymphocytic leukemia: still a matter of debate. Leuk Lymphoma (2019) 60:867–75. doi: 10.1080/10428194.2018.1509317
11
BurgerJAGribbenJG. The microenvironment in chronic lymphocytic leukemia (CLL) and other B cell malignancies: Insight into disease biology and new targeted therapies. Semin Cancer Biol (2014) 24:71–81. doi: 10.1016/j.semcancer.2013.08.011
12
PlanderMSeegersSUgocsaiPDiermeier-DaucherSIványiJSchmitzGet al. Different proliferative and survival capacity of CLL-cells in a newly established in vitro model for pseudofollicles. Leukemia (2009) 23:2118–28. doi: 10.1038/leu.2009.145
13
GhamlouchHOuled-HaddouHDamajGRoyerBGublerBMarolleauJ-P. A Combination of Cytokines Rescues Highly Purified Leukemic CLL B-Cells from Spontaneous Apoptosis In Vitro. PLoS One (2013) 8:e60370. doi: 10.1371/journal.pone.0060370
14
LongMBeckwithKDoPMundyBLGordonALehmanAMet al. Ibrutinib treatment improves T cell number and function in CLL patients. J Clin Invest (2017) 127:3052–64. doi: 10.1172/JCI89756
15
van AttekumMHAElderingEKaterAP. Chronic lymphocytic leukemia cells are active participants in microenvironmental cross-talk. Haematologica (2017) 102:1469–76. doi: 10.3324/haematol.2016.142679
16
KaechSMAhmedR. Memory CD8+ T cell differentiation: Initial antigen encounter triggers a developmental program in naïve cells. Nat Immunol (2001) 2:415–22. doi: 10.1038/87720
17
BajénoffMGranjeaudSGuerderS. The strategy of T cell antigen-presenting cell encounter in antigen-draining lymph nodes revealed by imaging of initial T cell activation. J Exp Med (2003) 198:715–24. doi: 10.1084/jem.20030167
18
LambleAJLindEF. Targeting the immune microenvironment in acute myeloid leukemia: A focus on T cell immunity. Front Oncol (2018) 8:213. doi: 10.3389/fonc.2018.00213
19
BagnaraDKaufmanMSCalissanoCMarsilioSPattenPEMSimoneRet al. A novel adoptive transfer model of chronic lymphocytic leukemia suggests a key role for T lymphocytes in the disease. Blood (2011) 117:5463–72. doi: 10.1182/blood-2010-12-324210
20
PattenPEMFerrerGChenS-SSimoneRMarsilioSYanX-Jet al. Chronic lymphocytic leukemia cells diversify and differentiate in vivo via a nonclassical Th1-dependent, Bcl-6–deficient process. JCI Insight (2016) 1(4):e86288. doi: 10.1172/jci.insight.86288
21
BaliakasPJerominSIskasMPuiggrosAPlevovaKNguyen-KhacFet al. Cytogenetic complexity in chronic lymphocytic leukemia: Definitions, associations, and clinical impact. Blood (2019) 133:1205–16. doi: 10.1182/blood-2018-09-873083
22
SchumacherTNSchreiberRD. Neoantigens in cancer immunotherapy. Science (80- ) (2015) 348:69–74. doi: 10.1126/science.aaa4971
23
TauschEMertensDStilgenbauerS. Genomic features: Impact on pathogenesis and treatment of chronic lymphocytic leukemia. Oncol Res Treat (2016) 39:34–40. doi: 10.1159/000443906
24
GaidanoGRossiD. The mutational landscape of chronic lymphocytic leukemia and its impact on prognosis and treatment. Hematology (2017) 2017:329–37. doi: 10.1182/asheducation-2017.1.329
25
RichesJCDaviesJKMcClanahanFFatahRIqbalSAgrawalSet al. T cells from CLLpatients exhibit features of T-cell exhaustion but retain capacity for cytokine production. Blood (2013) 121:1612–21. doi: 10.1182/blood-2012-09-457531
26
GitelsonEHammondCMenaJLorenzoMBucksteinRBerinsteinNLet al. Chronic lymphocytic leukemia-reactive T cells during disease progression and after autologous tumor cell vaccines. Clin Cancer Res (2003) 9:1656–65.
27
NicholasNSApollonioBRamsayAG. Tumor microenvironment (TME)-driven immune suppression in B cell malignancy. Biochim Biophys Acta - Mol Cell Res (2016) 1863:471–82. doi: 10.1016/j.bbamcr.2015.11.003
28
RovidaAMaccalliCScarfòLDellabonaPStamatopoulosKGhiaP. Exploiting B Cell Receptor Stereotypy to design Tailored Immunotherapy in Chronic Lymphocytic Leukemia. Clin Cancer Res (2020). doi: 10.1158/1078-0432.CCR-20-1632
29
KhodadoustMSOlssonNWagarLEHaabethOAWChenBSwaminathanKet al. Antigen presentation profiling reveals recognition of lymphoma immunoglobulin neoantigens. Nature (2017) 543:723–7. doi: 10.1038/nature21433
30
ScrivenerSGoddardRVKaminskiERPrenticeAG. Abnormal T-cell function in B-cell chronic lymphocytic leukaemia. Leuk Lymphoma (2003) 44:383–9. doi: 10.1080/1042819021000029993
31
GörgünGHolderriedTAWZahriehDNeubergDGribbenJG. Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells. J Clin Invest (2005) 115:1797–805. doi: 10.1172/JCI24176
32
NunesCWongRMasonMFeganCManSPepperC. Expansion of a CD8 +PD-1 + replicative senescence phenotype in early stage CLL patients is associated with inverted CD4:CD8 ratios and disease progression. Clin Cancer Res (2012) 18:678–87. doi: 10.1158/1078-0432.CCR-11-2630
33
HannaBSRoessnerPMYazdanparastHColomerDCampoEKuglerSet al. Control of chronic lymphocytic leukemia development by clonally-expanded CD8 + T-cells that undergo functional exhaustion in secondary lymphoid tissues. Leukemia (2019) 33:625–37. doi: 10.1038/s41375-018-0250-6
34
BuschleMCampanaDCardingSRRichardCVictor HoffbrandABrennerMK. Interferon γ inhibits apoptotic cell death in B cell chronic lymphocytic leukemia. J Exp Med (1993) 177:213–8. doi: 10.1084/jem.177.1.213
35
Del GiudiceIChiarettiSTavolaroSDe ProprisMSMaggioRManciniFet al. Spontaneous regression of chronic lymphocytic leukemia: Clinical and biologic features of 9 cases. Blood (2009) 114:638–46. doi: 10.1182/blood-2008-12-196568
36
CatakovicKGassnerFJRatswohlCZaborskyNRebhandlSSchubertMet al. TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia. Oncoimmunology (2018) 7(1):e1371399. doi: 10.1080/2162402X.2017.1371399
37
ElstonLFeganCHillsRHashimdeenSSWalsbyEHenleyPet al. Increased frequency of CD4+PD-1+HLA-DR+ T cells is associated with disease progression in CLL. Br J Haematol (2020) 188:872–80. doi: 10.1111/bjh.16260
38
Gonzalez-RodriguezAPContestiJHuergo-ZapicoLLopez-SotoAFernández-GuiznAAcebes-HuertaAet al. Prognostic significance of CD8 and CD4 T cells in chronic lymphocytic leukemia. Leuk Lymphoma (2010) 51:1829–36. doi: 10.3109/10428194.2010.503820
39
TakeuchiYNishikawaH. Roles of regulatory T cells in cancer immunity. Int Immunol (2016) 28:401–9. doi: 10.1093/intimm/dxw025
40
PiperKPKaranthMMcLarnonAKalkEKhanNMurrayJet al. Chronic lymphocytic leukaemia cells drive the global CD4 + T cell repertoire towards a regulatory phenotype and leads to the accumulation of CD4 + forkhead box P3 + T cells. Clin Exp Immunol (2011) 166:154–63. doi: 10.1111/j.1365-2249.2011.04466.x
41
LadDHoeppliRHuangQGarciaRXuLTozeCet al. Regulatory T-cells drive immune dysfunction in CLL. Leuk Lymphoma (2018) 59:486–9. doi: 10.1080/10428194.2017.1330475
42
BeyerMClassenSEndlEKochanekMWeihrauchMRDebey-PascherSet al. Comparative approach to define increased regulatory T cells in different cancer subtypes by combined assessment of CD127 and FOXP3. Clin Dev Immunol (2011) 11:734036. doi: 10.1155/2011/734036
43
SakaguchiS. Regulatory T Cells: Key Controllers of Immunologic Self-Tolerance. Cell (2000) 101:455–8. doi: 10.1016/s0092-8674(00)80856-9
44
JakMMousRRemmerswaalEBMSpijkerRJaspersAYagüeAet al. Enhanced formation and survival of CD4+ CD25hi Foxp3+ T-cells in chronic lymphocytic leukemia. Leuk Lymphoma (2009) 50:788–801. doi: 10.1080/10428190902803677
45
RobertsAWDavidsMSPagelJMKahlBSPuvvadaSDGerecitanoJFet al. Targeting BCL2 with Venetoclax in Relapsed Chronic Lymphocytic Leukemia. N Engl J Med (2016) 374:311–22. doi: 10.1056/NEJMoa1513257
46
FischerKAl-SawafOBahloJFinkA-MTandonMDixonMet al. Venetoclax and Obinutuzumab in Patients with CLL and Coexisting Conditions. N Engl J Med (2019) 380:2225–36. doi: 10.1056/NEJMoa1815281
47
TriulziTTagliabueEBalsariACasaliniP. FOXP3 expression in tumor cells and implications for cancer progression. J Cell Physiol (2013) 228:30–5. doi: 10.1002/jcp.24125
48
KaranikasVSpeletasMZamanakouMKalalaFLoulesGKerenidiTet al. Foxp3 expression in human cancer cells. J Transl Med (2008) 6:19. doi: 10.1186/1479-5876-6-19
49
NagaiYLamLGreeneMIZhangH. FOXP3 and Its Cofactors as Targets of Immunotherapies. Engineering (2019) 5:115–21. doi: 10.1016/j.eng.2019.01.001
50
LozanoTGorraizMLasarte-CíaARuizMRabalOOyarzabalJet al. Blockage of FOXP3 transcription factor dimerization and FOXP3/AML1 interaction inhibits T regulatory cell activity: sequence optimization of a peptide inhibitor. Oncotarget (2017) 8:71709–24. doi: 10.18632/oncotarget.17845
51
YousefiMMovassaghpourAAShamsasenjanKGhalamfarsaGSadreddiniSJadidi-NiaraghFet al. The skewed balance between Tregs and Th17 in chronic lymphocytic leukemia. Futur Oncol (2015) 11:1567–82. doi: 10.2217/fon.14.298
52
HusIBojarska-JunakAChocholskaSTomczakWWośJDmoszyńskaAet al. Th17/IL-17A might play a protective role in chronic lymphocytic leukemia immunity. PLoS One (2013) 8:1–8. doi: 10.1371/journal.pone.0078091
53
Jadidi-NiaraghFGhalamfarsaGMemarianAAsgarian-OmranHRazaviSMSarrafnejadAet al. Downregulation of IL-17-producing T cells is associated with regulatory T cell expansion and disease progression in chronic lymphocytic leukemia. Tumor Biol (2013) 34:929–40. doi: 10.1007/s13277-012-0628-4
54
BoćkoDKosmaczewskaACiszakLTeodorowskaRFrydeckaI. CD28 costimulatory molecule--expression, structure and function. Arch Immunol Ther Exp (Warsz) (2020) 50(3):169–77.
55
BonyhadiMFrohlichMRasmussenAFerrandCGrosmaireLRobinetEet al. In Vitro Engagement of CD3 and CD28 Corrects T Cell Defects in Chronic Lymphocytic Leukemia. J Immunol (2005) 174:2366–75. doi: 10.4049/jimmunol.174.4.2366
56
ReichardtPDornbachBGunzerM. APC, T cells, and the immune synapse. Curr Top Microbiol Immunol (2010) 340:229–49. doi: 10.1007/978-3-642-03858-7_12
57
Riches JCRamsay AGG. GribbenJ. Immune Dysfunction in Chronic Lymphocytic Leukemia: The Role for Immunotherapy. Curr Pharm Des (2012) 18:3389–98. doi: 10.2174/138161212801227023
58
McClanahanFHannaBMillerSClearAJLichterPGribbenJGet al. PD-L1 checkpoint blockade prevents immune dysfunction and leukemia development in a mouse model of chronic lymphocytic leukemia. Blood (2015) 126:203–11. doi: 10.1182/blood-2015-01-622936
59
GorgunGRamsayAGHolderriedTAWZahriehDDieuRLiuFet al. Eμ- TCL1 mice represent a model for immunotherapeutic reversal of chronic lymphocytic leukemia-induced T-cell dysfunction. Proc Natl Acad Sci U S A (2009) 106:6250–5. doi: 10.1073/pnas.0901166106
60
PalmaMGentilcoreGHeimerssonKMozaffariFNäsman-GlaserBYoungEet al. Mellstedt H. T cells in chronic lymphocytic leukemia display dysregulated expression of immune checkpoints and activation markers. Haematologica (2017) 102:562–72. doi: 10.3324/haematol.2016.151100
61
RamsayAGClearAJFatahRGribbenJG. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood (2012) 120(7):1412–21. doi: 10.1182/blood-2012-02-411678
62
DarvinPToorSMSasidharan NairVElkordE. Immune checkpoint inhibitors: recent progress and potential biomarkers. Exp Mol Med (2018) 50:165. doi: 10.1038/s12276-018-0191-1
63
HuBJacobsRGhoshN. Checkpoint Inhibitors Hodgkin Lymphoma and Non-Hodgkin Lymphoma. Curr Hematol Malig Rep (2018) 13:543–54. doi: 10.1007/s11899-018-0484-4
64
DingWLaPlantBRCallTGParikhSALeisJFHeRet al. Pembrolizumab in patients with CLL and Richter transformation or with relapsed CLL. Blood (2017) 129:3419–27. doi: 10.1182/blood-2017-02-765685
65
Xu-MonetteZYZhouJYoungKH. PD-1 expression and clinical PD-1 blockade in B-cell lymphomas. Blood (2018) 131:68–83. doi: 10.1182/blood-2017-07-740993
66
Chanan-KhanAPorterCW. Immunomodulating drugs for chronic lymphocytic leukaemia. Lancet Oncol (2006) 7:480–8. doi: 10.1016/S1470-2045(06)70723-9
67
CarballidoEVelizMKomrokjiRPinilla-IbarzJ. Immunomodulatory drugs and active immunotherapy for chronic lymphocytic leukemia. Cancer Control (2012) 19(1):54–67. doi: 10.1177/107327481201900106
68
RamsayAGEvansRKiaiiSSvenssonLHoggNGribbenJG. Chronic lymphocytic leukemia cells induce defective LFA-1-directed T-cell motility by altering Rho GTPase signaling that is reversible with lenalidomide. Blood (2013) 121:2704–14. doi: 10.1182/blood-2012-08-448332
69
RamsayAGJohnsonAJLeeAMGorgünGLe DieuRBlumWet al. Chronic lymphocytic leukemia T cells show impaired immunological synapse formation that can be reversed with an immunomodulating drug. J Clin Invest (2008) 118(7):2427–37. doi: 10.1172/jci35017
70
ShanafeltTDRamsayAGZentCSLeisJFTunHWCallTGet al. Long-term repair of T-cell synapse activity in a phase II trial of chemoimmunotherapy followed by lenalidomide consolidation in previously untreated chronic lymphocytic leukemia (CLL). Blood (2013) 121:4137–41. doi: 10.1182/blood-2012-12-470005
71
SakuishiKApetohLSullivanJMBlazarBRKuchrooVKAndersonAC. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med (2010) 207:2187–94. doi: 10.1084/jem.20100643
72
BlankCKuballJVoelklSWiendlHBeckerBWalterBet al. Blockade of PD-L1 (B7-H1) augments human tumor-specific T cell responses in vitro. Int J Cancer (2006) 119:317–27. doi: 10.1002/ijc.21775
73
KlebanoffCARosenbergSARestifoNP. Prospects for gene-engineered T cell immunotherapy for solid cancers. Nat Med (2016) 22:26–36. doi: 10.1038/nm.4015
74
HannaBSRoessnerPMScheffoldAJebarajBMCDemerdashYÖztürkSet al. PI3Kδ inhibition modulates regulatory and effector T-cell differentiation and function in chronic lymphocytic leukemia. Leukemia (2019) 33:1427–38. doi: 10.1038/s41375-018-0318-3
75
NordeWJHoboWVan Der VoortRDolstraH. Coinhibitory molecules in hematologic malignancies: Targets for therapeutic intervention. Blood (2012) 120:728–36. doi: 10.1182/blood-2012-02-412510
76
VirginHWWherryEJAhmedR. Redefining Chronic Viral Infection. Cell (2009) 138:30–50. doi: 10.1016/j.cell.2009.06.036
77
WherryEJ. T cell exhaustion. Nat Immunol (2011) 12:492–9. doi: 10.1038/ni.2035
78
ZenzT. Exhausting T cells in CLL. Blood (2013) 121:1485–6. doi: 10.1182/blood-2013-01-475939
79
Llaó CidLHannaBSIskarMRoessnerPMÖztürkSLichterPet al. CD8+ T-cells of CLL-bearing mice acquire a transcriptional program of T-cell activation and exhaustion. Leuk Lymphoma (2020) 61:351–6. doi: 10.1080/10428194.2019.1660972
80
ShapiroMHerishanuYBen-Zion-katzDezorellaNSunCKaySet al. Lymphocyte activation gene 3: A novel therapeutic target in chronic lymphocytic leukemia. Haematologica (2017) 102:874–82. doi: 10.3324/haematol.2016.148965
81
VardiAStamatopoulosKHadzidimitriouA. T cells in chronic lymphocytic leukemia: Can they fight? Oncotarget (2017) 8:99209–10. doi: 10.18632/oncotarget.22277
82
GreavesM. Clonal expansion in B-CLL: Fungal drivers or self-service. J Exp Med (2013) 210:1–3. doi: 10.1084/jem.20122739
83
OsABürglerSRibesAPFunderudAWangDThompsonKMet al. Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep (2013) 4:566–77. doi: 10.1016/j.celrep.2013.07.011
84
MiniciCGounariMÜbelhartRScarfòLDühren-von MindenMSchneiderDet al. Distinct homotypic B-cell receptor interactions shape the outcome of chronic lymphocytic leukaemia. Nat Commun (2017) 8(1):15746. doi: 10.1038/ncomms15746
85
BurgerJATedeschiABarrPMRobakTOwenCGhiaPet al. Ibrutinib as Initial Therapy for Patients with Chronic Lymphocytic Leukemia. N Engl J Med (2015) 373:2425–37. doi: 10.1056/NEJMoa1509388
86
BrownJRByrdJCCoutreSEBensonDMFlinnIWWagner-JohnstonNDet al. Idelalisib, an inhibitor of phosphatidylinositol 3-kinase p110δ, for relapsed/refractory chronic lymphocytic leukemia. Blood (2014) 123:3390–7. doi: 10.1182/blood-2013-11-535047
87
WiestnerA. BCR pathway inhibition as therapy for chronic lymphocytic leukemia and lymphoplasmacytic lymphoma. Hematology (2014) 2014:125–34. doi: 10.1182/asheducation-2014.1.125
88
FaisFGhiottoFHashimotoSSellarsBValettoAAllenSLet al. Chronic lymphocytic leukemia B cells express restricted sets of mutated and unmutated antigen receptors. J Clin Invest (1998) 102:1515–25. doi: 10.1172/JCI3009
89
BaliakasPHadzidimitriouASuttonLAMingaEAgathangelidisANichelattiMet al. Clinical effect of stereotyped B-cell receptor immunoglobulins in chronic lymphocytic leukaemia: A retrospective multicentre study. Lancet Haematol (2014) 1:e74–84. doi: 10.1016/S2352-3026(14)00005-2
90
DamleRNWasilTFaisFGhiottoFValettoAAllenSLet al. Ig V Gene Mutation Status and CD38 Expression As Novel Prognostic Indicators in Chronic Lymphocytic Leukemia. Blood (1999) 94:1840–7. doi: 10.1182/blood.v94.6.1840
91
HamblinTJDavisZGardinerAOscierDGStevensonFK. Unmutated Ig V(H) genes are associated with a more aggressive form of chronic lymphocytic leukemia. Blood (1999) 94:1848–54. doi: 10.1182/blood.v94.6.1848
92
DeganMBombenRBoMDZucchettoANanniPRupoloMet al. Analysis of IgVH gene mutations in B cell chronic lymphocytic leukaemia according to antigen-driven selection identifies subgroups with different prognosis and usage of the canonical somatic hypermutation machinery. Br J Haematol (2004) 126:29–42. doi: 10.1111/j.1365-2141.2004.04985.x
93
StamatopoulosKAgathangelidisARosenquistRGhiaP. Antigen receptor stereotypy in chronic lymphocytic leukemia. Leukemia (2017) 31:282–91. doi: 10.1038/leu.2016.322
94
WenTMellstedtHJondalM. Presence of clonal T cell populations in chronic B lymphocytic leukemia and smoldering myeloma. J Exp Med (1990) 171:659–66. doi: 10.1084/jem.171.3.659
95
RezvanyMRJeddi-TehraniMÖsterborgAKimbyEWigzellHMellstedtH. Oligoclonal TCRBV gene usage in B-cell chronic lymphocytic leukemia: Major perturbations are preferentially seen within the CD4 T-cell subset. Blood (1999) 94:1063–9. doi: 10.1182/blood.v94.3.1063.415a17_1063_1069
96
FaraceFOrlanducciFDietrichPYGaudinCAngevinECourtierMHet al. T cell repertoire in patients with B chronic lymphocytic leukemia: Evidence for multiple in vivo T cell clonal expansions. J Immunol (1994) 153:4281–90.
97
AlatrakchiNFaraceFFrauECardePMunckJNTriebelF. T-cell clonal expansion in patients with B-cell lymphoproliferative disorders. J Immunother (1998) 21:363–70. doi: 10.1097/00002371-199809000-00004
98
RezvanyMRJeddi-TehraniMWigzellHÖsterborgAMellstedtH. Leukemia-associated monoclonal and oligoclonal TCR-BV use in patients with B-cell chronic lymphocytic leukemia. Blood (2003) 101:1063–70. doi: 10.1182/blood-2002-03-0746
99
HofbauerJPHeyderCDenkUKocherTHollerCTrapinDet al. Development of CLL in the TCL1 transgenic mouse model is associated with severe skewing of the T-cell compartment homologous to human CLL. Leukemia (2011) 25:1452–8. doi: 10.1038/leu.2011.111
100
VardiAAgathangelidisAStalikaEKarypidouMSiorentaAAnagnostopoulosAet al. Antigen Selection Shapes the T-cell Repertoire in Chronic Lymphocytic Leukemia. Clin Cancer Res (2016) 22:167–74. doi: 10.1158/1078-0432.CCR-14-3017
101
VardiAVlachonikolaEKarypidouMStalikaEBikosVGemenetziKet al. Restrictions in the T-cell repertoire of chronic lymphocytic leukemia: High-throughput immunoprofiling supports selection by shared antigenic elements. Leukemia (2017) 31:1555–61. doi: 10.1038/leu.2016.362
102
VardiAVlachonikolaEPapazoglouDPsomopoulosFKottaKIoannouNet al. T cell dynamics in chronic lymphocytic leukemia under different treatment modalities. Clin Cancer Res (2020) 26:clincanres.3827.2019. doi: 10.1158/1078-0432.CCR-19-3827
103
YinQSivinaMRobinsHYuskoEVignaliMO’BrienSet al. Ibrutinib Therapy Increases T Cell Repertoire Diversity in Patients with Chronic Lymphocytic Leukemia. J Immunol (2017) 198:1740–7. doi: 10.4049/jimmunol.1601190
104
RajasagiMShuklaSAFritschEFKeskinDBDeLucaDCarmonaEet al. Systematic identification of personal tumor-specific neoantigens in chronic lymphocytic leukemia. Blood (2014) 124:453–62. doi: 10.1182/blood-2014-04-567933
105
FritschEFRajasagiMOttPABrusicVHacohenNWuCJ. HLA-binding properties of tumor neoepitopes in humans. Cancer Immunol Res (2014) 2:522–9. doi: 10.1158/2326-6066.CIR-13-0227
106
KowalewskiDJStevanovicSRammenseeHGStickelJS. Antileukemia T-cell responses in CLL – We don’t need no aberration. Oncoimmunology (2015) 4:1–3. doi: 10.1080/2162402X.2015.1011527
107
KowalewskiDJSchusterHBackertLBerlinCKahnSKanzLet al. HLA ligandome analysis identifies the underlying specificities of spontaneous antileukemia immune responses in chronic lymphocytic leukemia (CLL). Proc Natl Acad Sci U S A (2015) 112:E116–75. doi: 10.1073/pnas.1416389112
108
KhodadoustMSOlssonNChenBSworderBShreeTLiuCLet al. B-cell lymphomas present immunoglobulin neoantigens. Blood (2019) 133:878–81. doi: 10.1182/blood-2018-06-845156
109
DunnGPBruceATIkedaHOldLJSchreiberRD. Cancer immunoediting: From immunosurveillance to tumor escape. Nat Immunol (2002) 3:991–8. doi: 10.1038/ni1102-991
110
ParmarSPatelKPinilla-IbarzJ. Ibrutinib (Imbruvica): A novel targeted therapy for chronic lymphocytic leukemia(2014). Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103574/ (Accessed August 10, 2020).
111
MhibikMWiestnerASunC. Harnessing the effects of BTKI on T cells for effective immunotherapy against CLL. Int J Mol Sci (2020) 21(1):68. doi: 10.3390/ijms21010068
112
DubovskyJABeckwithKANatarajanGWoyachJAJaglowskiSZhongYet al. Ibrutinib is an irreversible molecular inhibitor of ITK driving a Th1-selective pressure in T lymphocytes. Blood (2013) 122:2539–49. doi: 10.1182/blood-2013-06-507947
113
KondoKShaimHThompsonPABurgerJAKeatingMEstrovZet al. Ibrutinib modulates the immunosuppressive CLL microenvironment through STAT3-mediated suppression of regulatory B-cell function and inhibition of the PD-1/PD-L1 pathway. Leukemia (2018) 32:960–70. doi: 10.1038/leu.2017.304
114
Sagiv-BarfiIKohrtHEKCzerwinskiDKNgPPChangBYLevyR. Therapeutic antitumor immunity by checkpoint blockade is enhanced by ibrutinib, an inhibitor of both BTK and ITK. Proc Natl Acad Sci U S A (2015) 112:E966–72. doi: 10.1073/pnas.1500712112
115
UjjaniCWangHSkarbnikATrivediNRamziPKhanNet al. A phase 1 study of lenalidomide and ibrutinib in combination with rituximab in relapsed and refractory CLL. Blood Adv (2018) 2:762–8. doi: 10.1182/bloodadvances.2017015263
116
ChellappaSKushekharKMuntheLATjønnfjordGEAandahlEMOkkenhaugKet al. The PI3K p110δ Isoform Inhibitor Idelalisib Preferentially Inhibits Human Regulatory T Cell Function. J Immunol (2019) 202:1397–405. doi: 10.4049/jimmunol.1701703
117
De WeerdtIHoflandTDe BoerRDobberJADuboisJVan NieuwenhuizeDet al. Distinct immune composition in lymph node and peripheral blood of CLL patients is reshaped during venetoclax treatment. Blood Adv (2019) 3:2642–52. doi: 10.1182/bloodadvances.2019000360
118
GandiniSMassiDMandalàM. PD-L1 expression in cancer patients receiving anti PD-1/PD-L1 antibodies: A systematic review and meta-analysis. Crit Rev Oncol Hematol (2016) 100:88–98. doi: 10.1016/j.critrevonc.2016.02.001
119
WierzMPiersonSGuyonnetLViryELequeuxAOudinAet al. Dual PD1/LAG3 immune checkpoint blockade limits tumor development in a murine model of chronic lymphocytic leukemia. Blood (2018) 131:1617–21. doi: 10.1182/blood-2017-06-792267
120
AbkenHKoehlerPSchmidtPHombachAAHallekM. Engineered T cells for the adoptive therapy of b-cell chronic lymphocytic leukaemia. Adv Hematol (2012) 2012:595060. doi: 10.1155/2012/595060
121
CurranKJSeinstraBANikhaminYYehRUsachenkoYVan LeeuwenDGet al. Enhancing antitumor efficacy of chimeric antigen receptor T cells through constitutive CD40L expression. Mol Ther (2015) 23:769–78. doi: 10.1038/mt.2015.4
122
GargettTYuWDottiGYvonESChristoSNHayballJDet al. GD2-specific CAR T Cells Undergo Potent Activation and Deletion Following Antigen Encounter but can be Protected from Activation-induced Cell Death by PD-1 Blockade. Mol Ther (2016) 24:1135–49. doi: 10.1038/mt.2016.63
123
TorikaiHReikALiuPQZhouYZhangLMaitiSet al. A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR. Blood (2012) 119:5697–705. doi: 10.1182/blood-2012-01-405365
124
GiannopoulosKWłasiukPDmoszyńskaARolińskiJSchmittM. Peptide vaccination induces profound changes in the immune system in patients with B-cell chronic lymphocytic leukemia. Folia Histochem Cytobiol (2011) 49:161–7. doi: 10.5603/FHC.2011.0023
125
Van RooijNVan BuurenMMPhilipsDVeldsAToebesMHeemskerkBet al. Tumor exome analysis reveals neoantigen-specific T-cell reactivity in an ipilimumab-responsive melanoma. J Clin Oncol (2013) 31(32):e439–e42. doi: 10.1200/JCO.2012.47.7521
Summary
Keywords
chronic lymphocytic leukemia, T lymphocytes, anti-tumor immunity, tumor microenvironment, T cell-based therapies
Citation
Vlachonikola E, Stamatopoulos K and Chatzidimitriou A (2021) T Cells in Chronic Lymphocytic Leukemia: A Two-Edged Sword. Front. Immunol. 11:612244. doi: 10.3389/fimmu.2020.612244
Received
30 September 2020
Accepted
08 December 2020
Published
20 January 2021
Volume
11 - 2020
Edited by
Jérôme Paggetti, Luxembourg Institute of Health, Luxembourg
Reviewed by
Alan G. Ramsay, King’s College London, United Kingdom; Silvia Deaglio, University of Turin, Italy
Updates
Copyright
© 2021 Vlachonikola, Stamatopoulos and Chatzidimitriou.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Kostas Stamatopoulos, kostas.stamatopoulos@certh.gr
This article was submitted to Cancer Immunity and Immunotherapy, a section of the journal Frontiers in Immunology
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