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ORIGINAL RESEARCH article

Front. Immunol., 18 December 2020 | https://doi.org/10.3389/fimmu.2020.613194

Pharmaco-Optogenetic Targeting of TRPC Activity Allows for Precise Control Over Mast Cell NFAT Signaling

Bernadett Bacsa1, Annarita Graziani1, Denis Krivic1, Patrick Wiedner1, Roland Malli2, Thomas Rauter2, Oleksandra Tiapko1 and Klaus Groschner1*
  • 1Gottfried-Schatz-Research-Center–Biophysics, Medical University of Graz, Graz, Austria
  • 2Gottfried-Schatz-Research-Center–Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria

Canonical transient receptor potential (TRPC) channels are considered as elements of the immune cell Ca2+ handling machinery. We therefore hypothesized that TRPC photopharmacology may enable uniquely specific modulation of immune responses. Utilizing a recently established TRPC3/6/7 selective, photochromic benzimidazole agonist OptoBI-1, we set out to test this concept for mast cell NFAT signaling. RBL-2H3 mast cells were found to express TRPC3 and TRPC7 mRNA but lacked appreciable Ca2+/NFAT signaling in response to OptoBI-1 photocycling. Genetic modification of the cells by introduction of single recombinant TRPC isoforms revealed that exclusively TRPC6 expression generated OptoBI-1 sensitivity suitable for opto-chemical control of NFAT1 activity. Expression of any of three benzimidazole-sensitive TRPC isoforms (TRPC3/6/7) reconstituted plasma membrane TRPC conductances in RBL cells, and expression of TRPC6 or TRPC7 enabled light-mediated generation of temporally defined Ca2+ signaling patterns. Nonetheless, only cells overexpressing TRPC6 retained essentially low basal levels of NFAT activity and displayed rapid and efficient NFAT nuclear translocation upon OptoBI-1 photocycling. Hence, genetic modification of the mast cells’ TRPC expression pattern by the introduction of TRPC6 enables highly specific opto-chemical control over Ca2+ transcription coupling in these immune cells.

Introduction

Photopharmacology and optogenetics have emerged as experimental strategies that allow for exceptionally precise interference with tissue functions (1, 2). These technologies have proven particularly successful in elucidating basic principles of communication within complex signaling networks and are suggested as a prospective basis for light-mediated computer-cell/tissue interfaces in the context of synthetic biology (3). So far optogenetic and chemo-optogenetic have unequivocally made significant contributions to our understanding of neuronal circuits and provided important insights into the complex orchestration of immune reactions. Moreover, the feasibility of optogenetic manipulation of immune responses has repeatedly been demonstrated in a therapeutic context (47).

We have recently developed a new photopharmacological tool that allows for specific, light-assisted control over TRPC3/6/7 conductances in native tissues (8). Here we set out to test the suitability of this new approach for modulation of immune responses, using the well-characterized RBL-2H3 mast cell model. Mast cells are tissue-resident and confer innate and adaptive immune reactions, thereby playing a pivotal role in allergic disorders, cancer, and autoimmune diseases (9). Mast cells mediate IgE-dependent allergic reactions by release of inflammatory mediators via degranulation, production of inflammatory lipids, and production of cytokines (10) and have long been recognized as a critical component of the tumor microenvironment in a number of cancer types (1113). Based on the complex and essentially two-faced function of these tumor resident immune cells (12), therapeutic targeting of these cells requires uniquely specific approaches. High precision, local modulation of tumor-resident immune cells might represent a novel strategy for adjuvant immunotherapy in cancers (13). One approach to achieve sufficient specificity of immunomodulation is based on the finding that Ca2+ downstream signaling is strictly dependent on temporal signaling features (14, 15). Hence, the controlled sculpturing of immune cell Ca2+ signals is expected to enable control over immune responses in a uniquely specific manner. So far, the therapeutic modulation of immune cell functions by light has focused mainly on the major player in immune cell Ca2+ handling, the STIM/Orai Ca2+ entry complex (7). Nonetheless, other Ca2+ signaling elements may similarly serve as suitable targets for optical approaches. Although many other plasma membrane transporters and channels, including K+ channels and TRP channels are reportedly critical for immune cell activation (1618), these molecular players have so far not been considered and tested as targets.

For canonical transient receptor potential (TRPC) channels a contribution to Ca2+ signaling in immune cells has repeatedly been suggested (19). While several studies provide evidence for a contribution of TRPC1 and TRPC5 proteins to store-operated calcium signaling in rat (RBL-2H3) and mouse bone marrow-derived mast cells (BMMC), the exact function of TRPC isoforms in mast cells remains largely elusive (2022). Interestingly, TRPC3/6/7 protein complexes were found to interact with fyn kinase during FcϵRI-mediated mast cell activation (23). However, a more recent study argues against the contribution of TRPC3/6 channels to FcϵRI-mediated Ca2+ signaling in primary human lung mast cells as well as in LAD2 human mast cells, suggesting exclusively Orai but not TRPC may be considered as a target for the control of mast cells in allergic disease (24). Nonetheless, in clear contrast to a lack of linkage to FcϵRI stimulation, TRPC1, TRPC4, and TRPC6 proteins have been shown to confer downstream signaling in Mrgprb2-mediated mast cell activation of murine peritoneal mast cells (25). Thus, the efficacy and specificity of TRPC generated Ca2+ signals in terms of their coupling to downstream effectors might be strictly dependent on the mechanism and mode of activation.

In the present study, we explored the functional consequences of direct, lipid-independent control of TRPC3/6/7 conductances in RBL-2H3 mast cells by a new photochromic benzimidazole agonist (OptoBI-1). We report that overexpression of the TRPC6 isoform, and thus specific modification of the TRPC expression pattern of RBL-2H3 cells, allows for efficient and temporally precise control over NFAT1 signaling by light. Our results provide the first proof of concept for efficient chemo-genetic targeting of mast cell Ca2+ signaling and transcriptional regulation based on TRPC photopharmacology.

Methods

Reagents and Construct

All reagents used were of molecular biology grade, purchased from Sigma-Aldrich Handels GmbH unless specified otherwise (Vienna, Austria). CMV-R-GECO1.2 construct (#45494) was obtained from Addgene. The following constructs were generated: YFP-TRPC3, CFP-TRPC3, YFP-TRPC6, CFP-TRPC6-hTRPC3 (Q13507-3) and hTRPC6 (Q9Y210-1) genes were cloned into peYFP-C1 and peCFP-C1 vectors using EcoRI/XbaI cloning sites. The mCherry-NFAT1 was prepared by replacing CFP by mCherry in a CFP-NFAT (in peCFP-C1 vector) construct. The TRPC7-mseCFP construct (Q9WVC5-1 cloned into pCI-neo vector) was kindly provided by Prof. Yasuo Mori (Kyoto University, Japan). OptoBI-1 was synthesized at Bio-Techne (Bristol, GB).

Cell Culture and Genetic Manipulations

All experiments were performed in rat basophilic leukemia cells (RBL-2H3) cells, and no special ethical considerations were applied. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10% fetal bovine serum (FBS), streptomycin (100 μg/ml), penicillin (100 U/ml), L-glutamine (2 mmol/L), and HEPES (10 mmol/L). Cells were maintained in an incubator at 37°C, 5% CO2. Plasmids were delivered into RBL-2H3 cells by electroporation in a 4-mm electroporation cuvette (Bio-Rad, US), by adding plasmid (5 μg mCherry-NFAT1 and R-GECO; 20 μg TRPC constructs) to 4 × 106 cells in 400 μl Opti-MEM® and applying 200 V and 950 μF in a Gene Pulser II (Bio-Rad, UK). After electroporation cells were transferred from the cuvette onto coverslips in a 35 mm dish containing cell culture medium. Experiments were performed 16–24 h after electroporation.

RNA Isolation and Quantitative Real-Time PCR

RNA was isolated from cell lysates using EXTRACTME TOTAL RNA KIT with 1% ß-Mercaptoethanol (Blirt) and reverse transcribed using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA) in a thermal cycler (Bio-Rad) according to the manufacturer’s protocol. qPCR was performed using GoTaq® qPCR and RT-qPCR Mix (Promega) in a LightCycler C1000 Thermal Touch, Thermal Cycler CFX96™ Real Time System (Bio Rad). Relative expression of the target gene was normalized to rat ß-Actin as a reference gene. Specific Primers used for rat Trpc genes are listed below.

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Electrophysiology

Whole-cell electrophysiology was performed at room temperature. RBL-2H3 cells were seeded on coverslips 24 h prior to the experiments. Coverslips were mounted in a cell bath on an inverted Axiovert 200 microscope (Zeiss). YFP-TRPC3, YFP-TRPC6 and TRPC7-CFP transfected cells were identified by their green and blue fluorescence when illuminated at 500 nm or 436 nm using Oligochrome light source (FEI, Germany). Whole-cell measurements were performed using an Axopatch 200B amplifier (Molecular Devices) connected with a Digidata-1440A Digitizer (Axon Instruments). Signals were low-pass filtered at 2 kHz and digitized with 8 kHz. The application of linear voltage-ramp protocols ranging from −130 to +80 mV (holding potential 0 mV) was controlled by Clampex 11.0 (Axon Instruments) software. Currents at −90 and +70 mV were plotted against time and normalized by capacitance. Maximal current–voltage relationships from −130 to +80 mV were subtracted and normalized by capacitance. Illumination protocol for photo-pharmacological measurements was applied as for Ca2+ imaging. Patch pipettes were pulled from thin-wall filament glass capillaries (Harvard Apparatus, W3 30-0068), using a Sutter Instruments P1000 puller and BOX filaments FB245B to a resistance of 2–4 MΩ. Cells were kept in Tyrode solution during the experiments. Pipette solution contained (in mM): 120 cesium methanesulfonate, 20 CsCl, 15 HEPES, 5 MgCl2, 3 EGTA, titrated to pH 7.3 with CsOH. The osmolarity of all solutions was between 290 and 315 mOsm.

[Ca2+]i Imaging

Changes in intracellular Ca2+ ([Ca2+]i) were monitored using red-shifted genetically encoded Ca2+ sensor (R-GECO1.2). Briefly, RBL-2H3 cells overexpressing R-GECO with YFP-TRPC3, YFP-TRPC6 or TRPC7-CFP, were washed twice with experimental buffer (EB composition in mM: 140 NaCl, 5 KCl, 1 MgCl2, 10 HEPES, 10 glucose and 1 CaCl2 (pH 7.4, adjusted with NaOH). Coverslips were mounted in a cell bath containing EB and 10 µM OptoBI-1 on an inverted microscope (Olympus IX71, Germany) with 40 × 1.3 N.A. oil-immersion objective. Cells were excited to follow the R-GECO signal using 577/25 nm filters via TILL Oligochrome light source (FEI, Germany) and fluorescent images were captured every second at 632 nm (using 632/60 nm emission filter, Chroma Technology, VT, USA) with an ORCA-03G digital CCD camera (Hamamatsu, Japan) using Live Acquisition 2.6 software (FEI, Germany). The cis isomerization of OptoBI-1 compound was triggered by exposure to 10 s illumination period at 365 nm, and subsequent reversal of OptoBI-1 to trans conformation was achieved with 430 nm light exposure to 10 s period. The cycling of UV (365 nm; violet) and blue light (430 nm; blue) illuminations were repeated three times. The interval between each illumination cycle was 60 s. All experiments were performed at room temperature. Detailed photocycling protocol is described in (26).

NFAT Nuclear Translocation

Translocation of NFAT in mCherry-NFAT1 used as a control and YFP-TRPC3, YFP-TRPC6 and TRPC7-CFP overexpressed in RBL-2H3 cells was observed using an inverted microscope (Olympus IX71, Germany) equipped with a 40 × 1.3 NA oil immersion objective. During the recordings using Live Acquisition v2.6 software (TILL Photonics FEI Company, Gräfelfing Germany), the excitation of mCherry was achieved using 577/25 nm filter and fluorescent images were captured every 2 s at 632 nm (using 632/60 nm emission filter Chroma Technology, VT, USA) with an ORCA-05G digital CCD camera (Hamamatsu, Herrsching am Ammersee, Germany). ImageJ 1.51n software was used to measure the fluorescence intensity in the nucleus and cytoplasm before and after stimulation with 10 µM OptoBI-1. These values (nucleus/cytosol) were then plotted using the SigmaPlot 14.1 software (Systat Software Inc.). The translocation of mCherry-NFAT1 in RBL-2H3 cells was observed for 12 min.

Fluorescence Imaging

Images from RBL-2H3 cells overexpressing CFP-TRPC3, CFP-TRPC6 and TRPC7-CFP were taken with the Zeiss array confocal laser scanning microscope (ACLSM, Zeiss Axiovert 200 M) using a 100×/1.45 oil immersion objective (Zeiss Microsystems, Jena, Germany). Illumination at 445 nm with an argon laser system (series 543, CVI Melles Griot, CA, USA) and emissions collected with a CCD camera (CoolSnap HQ2, Photometrics, Tucson, Arizona, USA). Image analysis was performed in ImageJ open source imaging analysis software (https://fiji.sc/).

Statistical Analysis

Data analyses and graphical display were performed using Clampfit 11 (Axon Instruments) and SigmaPlot 14.1 (Systat Software Inc.). Data are presented as mean values ± S.E.M. Primarily, a Shapiro–Wilk test was conducted to test for normality of the value distribution. Whenever a normal distribution criterion was met, we used ANOVA to analyze the statistical significance. In general, differences were considered significant at p < 0.05 and indicated for individual comparisons in figures (* p < 0.05, ** p < 0.01, *** p < 0.001).

Results

Benzimidazole (OptoBI-1)-Mediated Control Over RBL-2H3 Mast Cell Ca2+ Signaling Requires Expression of Recombinant TRPC6 and TRPC7

Mammalian mast cells have been shown to express an array of TRPC gene products (27). Our initial experiments to explore the sensitivity of mast cell Ca2+ signaling to a new photochromic TRPC ligand (OptoBI-1, Figure 1), clearly indicated that endogenous expression of benzimidazole TRPC target channels (8) is below the threshold for effective photopharmacological intervention in RBL-2H3 mast cells. Nonetheless, our analysis of the expression profile for TRPC subtypes in RBL-2H3 cells revealed that besides TRPC1 and TRPC4 also two potential benzimidazole targets, i.e. TRPC3 and TRPC7, were expressed at the mRNA level, while TRPC6 expression was not detectable (Supplementary Figure 1). Consistent with the lack of Ca2+ signals generated in native RBL cells by OptoBI-1 photocycling (Figure 1; control), we failed to detect TRPC3 by immunoblotting. Unfortunately, a suitable antibody for TRPC7 detection is currently not available. Next, we attempted to reconstitute OptoBI-1 sensitive cation conductances in RBL-2H3 cells by overexpression of a single benzimidazole responsive TRPC proteins (TRPC3/6/7). Mast cells were genetically modified to express single OptoBI-1 target channels in combination with R-GECO as a reporter for cytosolic Ca2+ changes (8, 26). The overexpression of single TRPC proteins allowed us to investigate the consequences of photoactivation for reconstitution of each TRPC channel subtype in our mast cell model. Genetically modified RBL-2H3 cells were exposed to photocycling of OptoBI-1 to induce defined pattern of transient rises in cytosolic Ca2+ as shown in Figure 1. These changes were modest in cells overexpressing TRPC3 but profound in cells expressing TRPC6 or TRPC7. OptoBI-1-induced peak values of normalized R-GECO fluorescence in TRPC6 (3.29 ± 0.26; n = 23) and TRPC7 (4.4 ± 0.56; n = 21) overexpressing RBL cells remain below the maximum SOCE-mediated signals achieved by thapsigargin (1 µM, 6.2 ± 0.14; n = 7), given as a reference stimulus. Of note, both “on” and “off” kinetic of light-controlled Ca2+ changes were essentially fast allowing for precise control over signal frequency and duration. For TRPC6 the reversal of cellular Ca2+ levels upon channel deactivation was incomplete and showed a tendency to remain elevated above control levels between consecutive photocycles. Importantly, TRPC6 as well as TRPC7 expression enabled the light-mediated generation of temporally defined Ca2+ signaling pattern, with TRPC7 producing the largest UV light-induced rise in cytoplasmic Ca2+ during the first illumination cycle as well as the most prominent desensitization during consecutive photocyling.

FIGURE 1
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Figure 1 OptoBI-1 photocycling induced Ca2+ signaling triggered by overexpressed TRPC3, TRPC6 or TRPC7 channels in RBL-2H3 cells. Comparison of responses in cells overexpressing YFP-TRPC3, YFP-TRPC6 or TRPC7-CFP together with R-GECO as a Ca2+ reporter. Cells expressing R-GECO was only used as a control. Time courses (mean ± SEM) of R-GECO fluorescence intensity (red, 577 nm) during photoactivation of TRPC3 (black, n = 8 cells), TRPC6 (red, n = 23 cells), TRPC7 (gray, n = 21 cells), and controls (white, n = 10 cells). Cis-trans OptoBI-1 photocycling was repeated three times, by illuminating cells with UV (365 nm, 10 s; violet) followed by blue light (430 nm, 10 s, blue).

Recombinant TRPC3, TRPC6, and TRPC7 Are Similarly Targeted to the Plasma Membrane but Produce Divergent Levels of OptoBI-1-Sensitive Cation Conductances

To better understand the mechanistic basis of the observed differences in reconstitution of OptoBI-1 sensitive Ca2+ signaling by the individual TRPC isoforms in RBL-2H3 cells, we continued with examination of the cellular localization and characterization of the membrane conductances generated with recombinant TRPC3/6/7. As shown in Figure 2A and Supplementary Figure 2A, recombinant TRPC channels fused to fluorescent markers (CFP or YFP) were similarly targeted to the plasma membrane. All cells expressing a TRPC fusion construct exhibited clear plasma membrane localized fluorescence. Of note, modification of RBL-2H3 cells to overexpress single TRPC-CFP fusion constructs did not affect cell morphology (Figure 2A) or promoted signs of degranulation. The OptoBI-1-induced TRPC conductances were quantified by electrophysiology applying the same OptoBI-1 photocycling protocol as in experiments measuring cytoplasmic Ca2+ rises (Figure 2B). All three TRPC isoforms reconstituted cation conductances with features consistent with those recently described for OptoBI-1-activated recombinant channels in HEK293 cells (8). These features included the I–V characteristics (Figure 2C) and current inactivation/desensitization, consistent with previous reports on benzimidazole agonists as well as photochromic actuators (28, 29). In full agreement with the OptoBI-1-induced Ca2+signaling, recombinant TRPC3 produced the smallest conductance, while TRPC7 expressing cells showed the largest current density after photoactivation (Figure 2C). Hence, the superior impact of TRPC6 and TRPC7 on mast cell Ca2+ homeostasis corresponds to larger cation conductances generated by these isoforms in RBL-2H3 cells combined with the reported higher Ca2+ permeability as compared to TRPC3 (30).

FIGURE 2
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Figure 2 Overexpressed TRPC3/6/7 proteins are targeted to the plasma membrane of RBL-2H3 cells and generate cation conductances with divergent efficiency. (A) Representative epifluorescence images of RBL-2H3 cells expressing CFP-TRPC3 (n = 57), CFP-TRPC6 (n = 26) and TRPC7-CFP (n = 45), respectively. Scale bar = 10 µm. (B) Time courses of control (sham transfected, white, n = 6 cells), YFP-TRPC3 (black, n = 6 cells), YFP-TRPC6 (red, n = 8 cells) and TRPC7-CFP (grey, n = 8 cells) conductances recorded at −90 to +70 mV during repetitive photoconversion of OptoBI-1. Light illumination cycling is indicated as violet (365 nm) and blue (430 nm). (C) Representative net I–V relations (ImaxIbasal) of OptoBI-1 induced currents in YFP-TRPC3 (black), YFP-TRPC6 (red), TRPC7-CFP (dark grey) transfected RBL-2H3 cells applying voltage-ramp protocols. Insert: Current density of net, maximum responses obtained at −90 to +70 mV (mean ± SEM). Statistical significance was tested by two tailed t-test (normally distributed values) or Mann–Whitney tests (non-normally distributed values), *p < 0.05, if not indicated differences are not significant (p > 0.05).

Genetic Modification of RBL-2H3 Cells to Overexpress TRPC6 Enables Control Over Cellular NFAT Activity by Light

In a next step we explored whether the generation of OptoBI-1 sensitivity in RBL-2H3 by overexpression of a single TRPC isoform is suitable to gain control over downstream Ca2+-dependent gene transcription. We set out to test the concept of opto-chemical control over NFAT1 activity by genetic modification of the mast cell’s TRPC expression pattern. NFAT1 nuclear translocation was recorded by expressing mCherry-NFAT1 fusion protein as a reporter, and applying the above-described protocol of repetitive photoactivation (three flashes of UV illumination). The extent of NFAT activation was quantified before and 12 min after initiation of the channel activation/deactivation cycles. Basal NFAT1 translocation was generally increased in all cells modified to overexpress TRPC channels. Nonetheless, this increase was modest with TRPC6 expression (Figure 3A) and repetitive, transient activation of the TRPC6 conductance by light resulted in a robust and highly significant NFAT1 nuclear translocation (Figure 3 and Supplementary Video 1). By contrast cell expressing TRPC3 or TRPC7 displayed basal nuclear NFAT1 localization at levels comparable to that maximally achieved by TRPC6 activation. This phenomenon may be related to differences in basal channel activity, with an essentially low constitutive activity of TRPC6 (31), RBL cells expressing this channel subtype, displayed the lowest basal conductance measured immediately upon obtaining whole cell configuration in the absence of OptoBI-1 (Supplementary Figure 3). Importantly, only overexpression of TRPC6 channel in RBL-2H3 cells enabled precise control over mast cells Ca2+ transcription coupling by the photochromic benzimidazole OptoBI-1.

FIGURE 3
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Figure 3 TRPC6 expression enables OptoBI-1-mediated control of NFAT1 activity in RBL-2H3 cells by light. (A) NFAT1 nuclear translocation in RBL-2H3 cells overexpressing either mCherry-NFAT1 only (control; white, n = 30) or mCherry-NFAT1 along with YFP-TRPC3 (black, n=17), YFP-TRPC6 (red, n = 31) or with TRPC7-CFP (gray, n = 21). Mean ± SEM nucleus/cytosol fluorescence intensity ratio. The translocation of mCherry-NFAT1 was monitored during OptoBI-1 photoconversion, applying three photocycles of illumination (three Flashes) as indicated. Statistical significance was tested by One-Way ANOVA (Holm–Sidak test for normally distributed values) or One-Way ANOVA for Ranks (Dunn’s test for non-normally distributed values), *p < 0.05, ***p < 0.001, if not indicated—not significant (p > 0.05). (B) Images of NFAT1 translocation in RBL-2H3 cells expressing mCherry-NFAT1 only (control; white) or coexpressing mCherry-NFAT1 and YFP-TRPC3 (black), YFP-TRPC6 (red) or TRPC7-CFP (grey) before (0 min) and after (12 min) three cycles of UV (365 nm, 10 s) and blue light (430 nm, 10 s) illuminations. Scale bar represents 5 µm.

Discussion

With the present study, we provide evidence for practicability of chemo/pharmaco-optogenetic modulation of immune cells function. This strategy is based on targeting overexpressed TRPC6 channels by the photochromic benzimidazole OptoBI-1. We report proof of this concept by demonstrating control over NFAT1 activation in RBL-2H3 by light.

RBL-2H3 cells were found to express TRPC proteins, specifically TRPC3 and TRPC7, which reportedly confer sensitivity to benzimidazole photopharmacology (8). However, this endogenous TRPC expression pattern was insufficient to generate significant cellular Ca2+ signals and NFAT1 translocation in response to OptoBI-1 photocycling. Lack of benzimidazole sensitivity of native RBL-2H3 mast cells may be explained by essentially low TRPC expression at the protein level. TRPC3 protein was indeed barely detectable by immunoblotting (not shown), while a test for TRPC7 protein expression was hindered by the lack of an appropriate antibody. Nonetheless, it appears reasonable to assume that the expression of endogenous benzimidazole target channels (TRPC3/6/7) is below the threshold for coupling to the Ca2+/CaN/NFAT pathway. Of note, in certain cellular settings TRPC3-generated Ca2+ signals failed to serve as an upstream trigger signal for NFAT activation (32). Nonetheless, the coupling of TRPC activity to downstream effectors may be strictly dependent on their mode of activation (33). In a recent study, we were able to demonstrate the linkage of recombinant TRPC channels to the calcineurin (CaN)/NFAT pathway in HEK293 cells (26). Expression of a TRPC3 gain-of-function mutant displaying enhanced benzimidazole sensitivity, was found to enable control of NFAT activity by OptoBI-1 (26). Consequently, we explored the option to achieve high precision control over mast cell NFAT signaling by combining photopharmacology and genetic modification. To do so, we altered mast cell TRPC expression by individual overexpression of benzimidazole-sensitive TRPC isoforms. Overexpression of recombinant TRPC3, TRPC6, or TRPC7 channels in RBL-2H3 cells reconstituted TRPC conductances with a clear order of efficacy with the largest OptoBI-1-induced current densities measured in TRPC7 expressing cells, whereas recombinant TRPC3 produced only a modest benzimidazole-sensitive conductance. All recombinant channel isoforms were found well targeted to the mast cell plasma membrane. The reason for the substantial difference observed upon reconstitution of TRPC3 and TRPC7 in RBL-2H3 cells remains unclear. Light-activated TRPC conductances displayed marked inactivation/desensitization as reported previously for benzimidazole agonists as well as lipid actuators (28, 29). The prominent inactivation observed for TRPC7, may in part be explained by the high current density, considering a current- and/or Ca2+-dependent mechanism. Consistent with the higher Ca2+ selectivity of TRPC6 and TRPC7 channels, surmounting that of TRPC3 (34), OptoBI-1-photocycling exerted a profound impact on mast cell Ca2+ levels when cells expressed either TRPC6 or TRPC7, but not with TRPC3 expression. Thus, genetic modification of mast cells to overexpress TRPC6 or TRPC7 channels generated OptoBI-1 sensitivity that allows temporal sculpturing of Ca2+ signals in these immune cells. Notably, membrane currents and cytosolic Ca2+ levels did not decline synchronously upon fast, light-induced deactivation as previously reported for TRPC3 mutants in the HEK293 expression system (26). Remarkably long-lasting Ca2+ elevations were triggered by repetitive TRPC6 activation. This phenomenon may be related to cellular localization of these channels relative to major Ca2+ extrusion systems. Since TRPC6 has repeatedly been found co-localized with NCX1 (3537), it is tempting to speculate that Na+ loading during TRPC6 activation might counteract NCX1-mediated Ca2+ extrusion thereby generating tonic elevation of basal Ca2+ upon repetitive activation. Importantly, only the introduction of an expression pattern featuring TRPC6 as the prominent species, provided a basis for efficient optical control over NFAT activation in the mast cells. Of note, all three benzimidazole-sensitive isoforms were found to communicate with the CaN/NFAT pathway, albeit in a divergent manner. TRPC3 and TRPC7 overexpressing mast cells displayed significant constitutive levels of NFAT1 nuclear localization, indicating the generation of a tonic increase of NFAT dephosphorylation, due to Ca2+ signals that arise from the constitutive activity of the overexpressed TRPC channels. Constitutive inward currents generated by TRPC3 and TRPC7 expression may results in tonic NFAT1 activation and preclude further control of NFAT transcriptional activity by photocycling of OptoBI-1. In RBL cells overexpressing TRPC6, which displays essentially low constitutive activity (31), basal NFAT1 translocation remained close to controls, and short pulsatile activation-deactivation of TRPC6 channels by OptoBI-1 photocycling resulted in rapid and significant NFAT1 nuclear translocation (within 10 min—see video in Supplementary Video 1). It is important to note that OptoBI-1 activation of TRPC6 may generate channel features and local Ca2+ entry pattern different from those of TRPC channels activated in response to receptor-phospholipase C pathways. The complex cascades inevitably linked to receptor-mediated activation of TRPC channels have been reported to modify the coupling between TRPC and NFAT signaling in cardiac muscle (38). To this end, the molecular basis of the observed efficient linkage between OptoBI-1-activated TRPC6 conductances and NFAT1 nuclear translocation in RBL-2H3 remains elusive. Nonetheless, our results demonstrate the general ability of TRPC6 to serve an important function, which is executed mainly by STIM/Orai channels in immune cells. Importantly, NFAT1 nuclear translocation triggered by TRPC-mediated Ca2+ entry, both by constitutive channel activity as well as short, transient pulses of channel activation using OptoBI-1 photocycling is potentially devoid of eliciting excessive exocytosis/degranulation, and may thereby enable a more specific modulation of immune responses as expected from interventions targeting the STIM/Orai machinery. This concept may be of relevance for the development of highly specific interventions to modify immune reactions of mast cells but also other immune cells, which play a complex, dual role in cancer pathology. It remains to be clarified if the here described concept of immunomodulation by TRPC photopharmacology can be adopted for control of other immune cells and as a basis of therapeutic strategies.

Data Availability Statement

The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding author.

Author Contributions

BB: Ca2+ imaging, manuscript writing, data analysis. AG: Ca2+ imaging, NFAT translocation, data analysis. DK: NFAT translocation, data analysis. PW: qPCR, primer design. TR: fluorescence microscopy. RM: experimental design. OT: patch-clamp, data analysis. KG: concept, experimental design, funding, and manuscript writing. All authors contributed to the article and approved the submitted version.

Funding

This research was funded by FWF P3326 (KG). DK is a member of the PhD program (DK) “Metabolic and Cardiovascular Disease” [W1226].

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The handling editor declared a past co-authorship with one of the authors, RM.

Acknowledgments

The authors wish to thank Mrs. Michaela Janschitz for excellent technical assistance. We wish to thank Prof. Yasuo Mori providing us with the TRPC7-CFP construct and Bio-Techne for its support with synthesizing OptoBI-1.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2020.613194/full#supplementary-material

References

1. Velema WA, Szymanski W, Feringa BL. Photopharmacology: beyond proof of principle. J Am Chem Soc (2014) 136(6):2178–91. doi: 10.1021/ja413063e

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Ankenbruck N, Courtney T, Naro Y, Deiters A. Optochemical Control of Biological Processes in Cells and Animals. Angew Chem Int Ed Engl (2018) 57(11):2768–98. doi: 10.1002/anie.201700171

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Fehrentz T, Schonberger M, Trauner D. Optochemical genetics. Angew Chem Int Ed Engl (2011) 50(51):12156–82. doi: 10.1002/anie.201103236

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Ryu KA, McGonnigal B, Moore T, Kargupta T, Mancini RJ, Esser-Kahn AP. Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist. Sci Rep (2017) 7(1):8074. doi: 10.1038/s41598-017-08520-x

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Tan P, He L, Han G, Zhou Y. Optogenetic Immunomodulation: Shedding Light on Antitumor Immunity. Trends Biotechnol (2017) 35(3):215–26. doi: 10.1016/j.tibtech.2016.09.002

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Bohineust A, Garcia Z, Corre B, Lemaitre F, Bousso P. Optogenetic manipulation of calcium signals in single T cells in vivo. Nat Commun (2020) 11(1):1143. doi: 10.1038/s41467-020-14810-2

PubMed Abstract | CrossRef Full Text | Google Scholar

7. He L, Zhang Y, Ma G, Tan P, Li Z, Zang S, et al. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife (2015) 4:e10024. doi: 10.7554/eLife.10024

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Tiapko O, Shrestha N, Lindinger S, Guedes de la Cruz G, Graziani A, Klec C, et al. Lipid-independent control of endothelial and neuronal TRPC3 channels by light. Chem Sci (2019) 10(9):2837–42. doi: 10.1039/c8sc05536j

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Wedemeyer J, Tsai M, Galli SJ. Roles of mast cells and basophils in innate and acquired immunity. Curr Opin Immunol (2000) 12(6):624–31. doi: 10.1016/S0952-7915(00)00154-0

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Rivera J, Gilfillan AM. Molecular regulation of mast cell activation. J Allergy Clin Immunol (2006) 117(6):1214–25; quiz 26. doi: 10.1016/j.jaci.2006.04.015

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Komi DEA, Redegeld FA. Role of Mast Cells in Shaping the Tumor Microenvironment. Clin Rev Allergy Immunol (2020) 58(3):313–25. doi: 10.1007/s12016-019-08753-w

PubMed Abstract | CrossRef Full Text | Google Scholar

12. Aponte-Lopez A, Fuentes-Panana EM, Cortes-Munoz D, Munoz-Cruz S. Mast Cell, the Neglected Member of the Tumor Microenvironment: Role in Breast Cancer. J Immunol Res (2018) 2018:2584243. doi: 10.1155/2018/2584243

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Kaesler S, Wolbing F, Kempf WE, Skabytska Y, Koberle M, Volz T, et al. Targeting tumor-resident mast cells for effective anti-melanoma immune responses. JCI Insight (2019) 4(19):e125057. doi: 10.1172/jci.insight.125057

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Kar P, Parekh AB. Distinct spatial Ca2+ signatures selectively activate different NFAT transcription factor isoforms. Mol Cell (2015) 58(2):232–43. doi: 10.1016/j.molcel.2015.02.027

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Berry CT, May MJ, Freedman BD. STIM- and Orai-mediated calcium entry controls NF-kappaB activity and function in lymphocytes. Cell Calcium (2018) 74:131–43. doi: 10.1016/j.ceca.2018.07.003

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Panyi G, Beeton C, Felipe A. Ion channels and anti-cancer immunity. Philos Trans R Soc Lond B Biol Sci (2014) 369(1638):20130106. doi: 10.1098/rstb.2013.0106

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Feske S, Wulff H, Skolnik EY. Ion channels in innate and adaptive immunity. Annu Rev Immunol (2015) 33:291–353. doi: 10.1146/annurev-immunol-032414-112212

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Khalil M, Alliger K, Weidinger C, Yerinde C, Wirtz S, Becker C, et al. Functional Role of Transient Receptor Potential Channels in Immune Cells and Epithelia. Front Immunol (2018) 9:174. doi: 10.3389/fimmu.2018.00174

PubMed Abstract | CrossRef Full Text | Google Scholar

19. Freichel M, Almering J, Tsvilovskyy V. The Role of TRP Proteins in Mast Cells. Front Immunol (2012) 3:150:150. doi: 10.3389/fimmu.2012.00150

PubMed Abstract | CrossRef Full Text | Google Scholar

20. Ma HT, Peng Z, Hiragun T, Iwaki S, Gilfillan AM, Beaven MA. Canonical transient receptor potential 5 channel in conjunction with Orai1 and STIM1 allows Sr2+ entry, optimal influx of Ca2+, and degranulation in a rat mast cell line. J Immunol (2008) 180(4):2233–9. doi: 10.4049/jimmunol.180.4.2233

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Suzuki R, Liu X, Olivera A, Aguiniga L, Yamashita Y, Blank U, et al. Loss of TRPC1-mediated Ca2+ influx contributes to impaired degranulation in Fyn-deficient mouse bone marrow-derived mast cells. J Leukoc Biol (2010) 88(5):863–75. doi: 10.1189/jlb.0510253

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Cohen R, Torres A, Ma HT, Holowka D, Baird B. Ca2+ waves initiate antigen-stimulated Ca2+ responses in mast cells. J Immunol (2009) 183(10):6478–88. doi: 10.4049/jimmunol.0901615

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Sanchez-Miranda E, Ibarra-Sanchez A, Gonzalez-Espinosa C. Fyn kinase controls FcepsilonRI receptor-operated calcium entry necessary for full degranulation in mast cells. Biochem Biophys Res Commun (2010) 391(4):1714–20. doi: 10.1016/j.bbrc.2009.12.139

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Wajdner HE, Farrington J, Barnard C, Peachell PT, Schnackenberg CG, Marino JP Jr., et al. Orai and TRPC channel characterization in FcepsilonRI-mediated calcium signaling and mediator secretion in human mast cells. Physiol Rep (2017) 5(5). doi: 10.14814/phy2.13166

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Tsvilovskyy V, Solis-Lopez A, Almering J, Richter C, Birnbaumer L, Dietrich A, et al. Analysis of Mrgprb2 Receptor-Evoked Ca (2+) Signaling in Bone Marrow Derived (BMMC) and Peritoneal (PMC) Mast Cells of TRPC-Deficient Mice. Front Immunol (2020) 11:564:564. doi: 10.3389/fimmu.2020.00564

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Graziani A, Bacsa B, Krivic D, Wiedner P, Curcic S, Schindl R, et al. Light-Mediated Control over TRPC3-Mediated NFAT Signaling. Cells (2020) 9(3):556. doi: 10.3390/cells9030556

CrossRef Full Text | Google Scholar

27. Parenti A, De Logu F, Geppetti P, Benemei S. What is the evidence for the role of TRP channels in inflammatory and immune cells? Brit J Pharmacol (2016) 173(6):953–69. doi: 10.1111/bph.13392

CrossRef Full Text | Google Scholar

28. Lichtenegger M, Tiapko O, Svobodova B, Stockner T, Glasnov TN, Schreibmayer W, et al. An optically controlled probe identifies lipid-gating fenestrations within the TRPC3 channel. Nat Chem Biol (2018) 14(4):396–404. doi: 10.1038/s41589-018-0015-6

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Svobodova B, Lichtenegger M, Platzer D, Di Giuro CML, de la Cruz GG, Glasnov T, et al. A single point mutation in the TRPC3 lipid-recognition window generates supersensitivity to benzimidazole channel activators. Cell Calcium (2019) 79:27–34. doi: 10.1016/j.ceca.2019.02.007

PubMed Abstract | CrossRef Full Text | Google Scholar

30. Curcic S, Tiapko O, Groschner K. Photopharmacology and opto-chemogenetics of TRPC channels-some therapeutic visions. Pharmacol Ther (2019) 200:13–26. doi: 10.1016/j.pharmthera.2019.04.003

PubMed Abstract | CrossRef Full Text | Google Scholar

31. Dietrich A, Mederos y Schnitzler M, Emmel J, Kalwa H, Hofmann T. Gudermann T. N-linked protein glycosylation is a major determinant for basal TRPC3 and TRPC6 channel activity. J Biol Chem (2003) 278(48):47842–52. doi: 10.1074/jbc.M302983200

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Kar P, Nelson C, Parekh AB. Selective activation of the transcription factor NFAT1 by calcium microdomains near Ca2+ release-activated Ca2+ (CRAC) channels. J Biol Chem (2011) 286(17):14795–803. doi: 10.1074/jbc.M111.220582

PubMed Abstract | CrossRef Full Text | Google Scholar

33. Poteser M, Schleifer H, Lichtenegger M, Schernthaner M, Stockner T, Kappe CO, et al. PKC-dependent coupling of calcium permeation through transient receptor potential canonical 3 (TRPC3) to calcineurin signaling in HL-1 myocytes. P Natl Acad Sci USA (2011) 108(26):10556–61. doi: 10.1073/pnas.1106183108

CrossRef Full Text | Google Scholar

34. Lievremont JP, Bird GS, Putney JW. Canonical transient receptor potential TRPC7 can function as both a receptor- and store-operated channel in HEK-293 cells. Am J Physiol-Cell Ph (2004) 287(6):C1709–C16. doi: 10.1152/ajpcell.00350.2004

CrossRef Full Text | Google Scholar

35. Linde CI, Karashima E, Raina H, Zulian A, Wier WG, Hamlyn JM, et al. Increased arterial smooth muscle Ca2+ signaling, vasoconstriction, and myogenic reactivity in Milan hypertensive rats. Am J Physiol Heart Circ Physiol (2012) 302(3):H611–20. doi: 10.1152/ajpheart.00950.2011

PubMed Abstract | CrossRef Full Text | Google Scholar

36. Poburko D, Liao CH, Lemos VS, Lin E, Maruyama Y, Cole WC, et al. Transient receptor potential channel 6-mediated, localized cytosolic [Na+] transients drive Na+/Ca2+ exchanger-mediated Ca2+ entry in purinergically stimulated aorta smooth muscle cells. Circ Res (2007) 101(10):1030–8. doi: 10.1161/CIRCRESAHA.107.155531

PubMed Abstract | CrossRef Full Text | Google Scholar

37. Xu J, Yang Y, Xie R, Liu J, Nie X, An J, et al. The NCX1/TRPC6 Complex Mediates TGFbeta-Driven Migration and Invasion of Human Hepatocellular Carcinoma Cells. Cancer Res (2018) 78(10):2564–76. doi: 10.1158/0008-5472.CAN-17-2061

PubMed Abstract | CrossRef Full Text | Google Scholar

38. Poteser M, Schleifer H, Lichtenegger M, Schernthaner M, Stockner T, Kappe CO, et al. PKC-dependent coupling of calcium permeation through transient receptor potential canonical 3 (TRPC3) to calcineurin signaling in HL-1 myocytes (vol 108, pg 10556, 2011). P Natl Acad Sci USA (2011) 108(33):13876–8. doi: 10.1073/pnas.1111388108

CrossRef Full Text | Google Scholar

Keywords: canonical transient receptor potential channels, mast cells, opto-chemical immunomodulation, NFAT nuclear translocation, photopharmacology, OptoBI-1

Citation: Bacsa B, Graziani A, Krivic D, Wiedner P, Malli R, Rauter T, Tiapko O and Groschner K (2020) Pharmaco-Optogenetic Targeting of TRPC Activity Allows for Precise Control Over Mast Cell NFAT Signaling. Front. Immunol. 11:613194. doi: 10.3389/fimmu.2020.613194

Received: 01 October 2020; Accepted: 17 November 2020;
Published: 18 December 2020.

Edited by:

Susanna Zierler, Johannes Kepler University of Linz, Austria

Reviewed by:

Volodymyr Tsvilovskyy, Heidelberg University, Germany
Anant Parekh, University of Oxford, United Kingdom

Copyright © 2020 Bacsa, Graziani, Krivic, Wiedner, Malli, Rauter, Tiapko and Groschner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Klaus Groschner, klaus.groschner@medunigraz.at