In the original article, there was a mistake in Figure 8A, as published. We showed that we did not get expression of the FLaB1 subunit in Manilae L495 strain. In fact, the forward primer (designed according to the Fiocruz sequence) used to amplify the FlaB1 subunit has two mismatches within the Manilae sequence. We did the RT-PCR with the good primer and found an amplification, showing an enhanced expression at the stationary phase compared to the exponential phase, likewise the other FlaB subunits. The corrected Figure 8 appears below.
A correction has been made to Results, “FlaB mRNA Are Upregulated in Stationary Phase”.
“Of note, and different from other strains, the Manilae L495 flaB1 mRNA was undetectable” and a paragraph in the Discussion, “Furthermore, in Manilae L495…” are not accurate and should be both omitted.
In the original article, there was also mistake in Figure 6, Supplementary Figure 3, Supplementary Figure 4 and Supplementary Figure 6 and their legends as published. We realized a “slipping” of the names of FlaB subunits only in the figures of alignments and comparison of sequences (Figure 6, Sup Figure 3, Sup Figure 4 and Sup Figure 6). What we called FlaB1 in figures and respective legends is in fact FlaB4, FlaB2 is FlaB1, FlaB3 is FlaB2, and FlaB4 is FlaB3 (see corrected annotation in Additional table provided).
Figure 6
| 10788 | Patoc | Fiocruz L1-130 | Manilae | Verdun |
|---|---|---|---|---|
| flaA1 | LEPBIa2335 | LIC10788 | LMANv2_260046 | AKWP_v1_210009 |
| flaA2 | LEPBIa2336 | LIC10787 | LMANv2_260045 | AKWP_v1_210008 |
| flaB1 | LEPBIa1589 | LIC11531 | LMANv2_590023 | AKWP_v1_110067 |
| flaB2 | LEPBIa2133 | LIC11890 | LMANv2_260016 | AKWP_v1_110429 |
| flaB3 | LEPBIa2132 | LIC11889 | LMANv2_260015 | AKWP_v1_110428 |
| flaB4 | LEPBIa1872 | LIC11532 | LMANv2_590024 | AKWP_v1_110068 |
Additional Table Old annotations (published Frontiers Immunol 2020).
| 10788 | Patoc* | Fiocruz L1-130* | Manilae* | Verdun* |
|---|---|---|---|---|
| flaA1 | LEPBIa2335 | LIC10788 | LMANv2_260046 / LIMLP_13775 | AKWP_v1_210009 |
| flaA2 | LEPBIa2336 | LIC10787 | LMANv2_260045 / LIMLP_13780 | AKWP_v1_210008 |
| flaB1 | LEPBIa2133 | LIC11890 | LMANv2_260016 / LIMLP_09410 | AKWP_v1_110429 |
| flaB2 | LEPBIa2132 | LIC11889 | LMANv2_260015 /LIMLP_09405 | AKWP_v1_110428 |
| flaB3 | LEPBIa1872 | LIC11532 | LMANv2_590024 / LIMLP_07480 | AKWP_v1_110068 |
| flaB4 | LEPBIa1589 | LIC11531 | LMANv2_590023 / LIMLP_07475 | AKWP_v1_110067 |
New annotations consistent with studies from M Picardeau.
*Annotation of L. biflexa serovar Patoc strain Patoc 1 (Patoc), L. interrogans serovar Copenhageni strain Fiocruz L1-130 (Fiocruz L1-130), L. interrogans serovar Manilae strain L495 (Manilae), L. interrogans serovar Manilae UP-MMC-NIID LP (Manilae) (ref Satou et al. 2015 ; PMID: 26272567).
L. interrogans serovar Icterohaemorrhagiae Verdun LP (Verdun) in https://mage.genoscope.cns.fr/microscope/home/index.php.
However, the annotations used in Figures 7, 8 were correct and all the mutants used are correct. The corrected Figure 6 and its corrected legend appears below. The corrected Supplementary Figures 3, 4, and 6 can be accessed from the original article.
Figure 8
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s note
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Summary
Keywords
Leptospira, toll-like receptor, innate immunity, Flagelin genes, TLR5, mouse model
Citation
Holzapfel M, Bonhomme D, Cagliero J, Vernel-Pauillac F, d’Andon MF, Bortolussi S, Fiette L, Goarant C, Wunder Jr. EA, Picardeau M, Ko AI, Werling D, Matsui M, Boneca IG and Werts C (2022) Corrigendum: Escape of TLR5 recognition by Leptospira spp.: A rationale for atypical endoflagella. Front. Immunol. 13:932151. doi: 10.3389/fimmu.2022.932151
Received
29 April 2022
Accepted
25 May 2022
Published
08 November 2022
Volume
13 - 2022
Edited and reviewed by
Melissa Jo Caimano, University of Connecticut Health Center, United States
Updates
Copyright
© 2022 Holzapfel, Bonhomme, Cagliero, Vernel-Pauillac, d’Andon, Bortolussi, Fiette, Goarant, Wunder, Picardeau, Ko, Werling, Matsui, Boneca and Werts.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Catherine Werts, cwerts@pasteur.fr
‡Present address: Laurence Fiette, IMMR, Paris, France
This article was submitted to Microbial Immunology, a section of the journal Frontiers in Immunology
†These authors share first authorship
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.