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CORRECTION article

Front. Immunol., 24 October 2025

Sec. Cancer Immunity and Immunotherapy

Volume 16 - 2025 | https://doi.org/10.3389/fimmu.2025.1709624

Correction: Exposing the cellular situation: findings from single-cell RNA sequencing in breast cancer

This article is a correction to:

  1. Exposing the cellular situation: findings from single cell RNA sequencing in breast cancer

    1. Read original article
Gaofeng Ni&#x;Gaofeng Ni1†Xinhan Li&#x;Xinhan Li2†Wenyang Nie&#x;Wenyang Nie2†Zhenzhen Zhao*Zhenzhen Zhao2*Hua Li,*Hua Li3,4*Hongyan Zang*Hongyan Zang1*
  • 1Department of Breast Surgery, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, China
  • 2The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China
  • 3Department of General Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China
  • 4Key Laboratory of Tumor Molecular Pathology of Baise, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China

A Correction on
Exposing the cellular situation: findings from single-cell RNA sequencing in breast cancer

By Ni G, Li X, Nie W, Zhao Z, Li H and Zang H (2025). Front. Immunol. 16:1539074. doi: 10.3389/fimmu.2025.1539074

There was a mistake in Figure 7F as published. We mistakenly wrote CEBPD in Figure 7F as EGFR. The corrected Figure 7F appears below.

Figure 7
The image is a multi-panel scientific figure related to breast cancer research. It includes various charts, graphs, and microscopy images examining the effects of si-NC, siCEBPD-1, and siCEBPD-2 treatments on BT-549 and MDA-MB-436 cell lines. Panels A and B display bar graphs and line charts of CEBPD RNA expression and cell growth over time. Panel C shows colony formation assays. Panels D, G, H, and I contain bar graphs depicting colony numbers, wound healing percentages, cell migration, and invasion. Panels E and F feature scratch assays and fluorescence microscopy images with DAPI and EDU staining. Statistical significance is indicated by asterisks.

Figure 7. In vitro experimental validation of CEBPD. (A) After CEBPD knock-down, the expression levels of mRNA and protein decreased significantly. (B) CCK-8 detection showed that compared with the control group, the cell viability was significantly decreased after CEBPD knock-down. (C, D) The colony formation assay showed that the number of colonies decreased significantly after CEBPD was knocked down. (E) Scratch test showed that CEBPD knockdown inhibited cell migration. (F) EDU staining confirmed that CEBPD knock-down had inhibitory effect on cell proliferation. (G) Bar chart showed that the ability of cell migration and proliferation decreased significantly after CEBPD. (H, I) Transwell experiment showed that CEBPD knockdown inhibited the migration and invasion of TCs in BT-549 and MDA-MB-436 cell lines. *, p < 0.05; **, p < 0.0 1; ***, p < 0.001 indicates a significant difference, and NS indicates a non-significant difference.

The original version of this article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: single cell RNA sequencing, breast cancer, CEBPD, transcription factors, tumor microenvironment, metabolism

Citation: Ni G, Li X, Nie W, Zhao Z, Li H and Zang H (2025) Correction: Exposing the cellular situation: findings from single-cell RNA sequencing in breast cancer. Front. Immunol. 16:1709624. doi: 10.3389/fimmu.2025.1709624

Received: 20 September 2025; Accepted: 17 October 2025;
Published: 24 October 2025.

Approved by:

Frontiers Editorial Office, Frontiers Media SA, Switzerland

Copyright © 2025 Ni, Li, Nie, Zhao, Li and Zang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Zhenzhen Zhao, MjAyMzExMDc5NUBzZHV0Y20uZWR1LmNu; Hua Li, bGlodWFfZ3hAeW11bi5lZHUuY24=; Hongyan Zang, MjQyMDA4Mjk4OEBxcS5jb20=

†These authors have contributed equally to this work and share first authorship

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.