%A Chen,Xiu P. %A Ali,Liaqat %A Wu,Li-Yun %A Liu,Can %A Gang,Chen X. %A Huang,Qi F. %A Ruan,Jing H. %A Bao,Song Y. %A Rao,Yun P. %A Yu,DaoJin %D 2018 %J Frontiers in Microbiology %C %F %G English %K Microcosm,Nitrogen,Phosphrus,Escherichia coli,antibiotic resistance,Biofilms,Biofilm-forming related genes,No observed effect concentration (NOEC) %Q %R 10.3389/fmicb.2018.00367 %W %L %M %P %7 %8 2018-March-02 %9 Original Research %+ DaoJin Yu,Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University,China,yudaojin@yeah.net %# %! Biofilm promote multidrug-resistant E. coli in eutrophic environment %* %< %T Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments %U https://www.frontiersin.org/articles/10.3389/fmicb.2018.00367 %V 9 %0 JOURNAL ARTICLE %@ 1664-302X %X In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on the multidrug resistance and biofilm-forming abilities of Escherichia coli. The expression of biofilm-formation-related genes was detected to establish correlations between genotype and phenotype. Different concentrations of N and P were added to make one control group and four treatment groups. The glass tube method was used to determine biofilm-forming capabilities. Real-time PCR was used to detect the mRNA abundance of six biofilm-formation-related genes in E. coli. No resistant strains were isolated from the control group; meanwhile, multidrug resistance rates were high in the treatment groups. Expression of the biofilm-associated genes luxS, flhD, fliA, motA, and fimH was detected in all treatment groups; however, there was no expression of mqsR. The expression of luxS, flhD, fliA, motA, and fimH significantly correlated with the concentration of N and P, as well as with the appearance and duration of multidrug resistance in different groups. Overall, the results of this study suggest that biofilm-forming ability plays a key role in the formation of multidrug resistance in E. coli after the addition of N and P to a microcosm.