%A Ingala,Melissa R. %A Simmons,Nancy B. %A Wultsch,Claudia %A Krampis,Konstantinos %A Speer,Kelly A. %A Perkins,Susan L. %D 2018 %J Frontiers in Microbiology %C %F %G English %K 16S rRNA,microbiome,field sampling methods,Metagenomics,Chiroptera %Q %R 10.3389/fmicb.2018.00803 %W %L %M %P %7 %8 2018-May-01 %9 Original Research %# %! Comparing Microbiome Sampling Methods in a Wild Mammal %* %< %T Comparing Microbiome Sampling Methods in a Wild Mammal: Fecal and Intestinal Samples Record Different Signals of Host Ecology, Evolution %U https://www.frontiersin.org/articles/10.3389/fmicb.2018.00803 %V 9 %0 JOURNAL ARTICLE %@ 1664-302X %X The gut microbiome is a community of host-associated symbiotic microbes that fulfills multiple key roles in host metabolism, immune function, and tissue development. Given the ability of the microbiome to impact host fitness, there is increasing interest in studying the microbiome of wild animals to better understand these communities in the context of host ecology and evolution. Human microbiome research protocols are well established, but wildlife microbiome research is still a developing field. Currently, there is no standardized set of best practices guiding the collection of microbiome samples from wildlife. Gut microflora are typically sampled either by fecal collection, rectal swabbing, or by destructively sampling the intestinal contents of the host animal. Studies rarely include more than one sampling technique and no comparison of these methods currently exists for a wild mammal. Although some studies have hypothesized that the fecal microbiome is a nested subset of the intestinal microbiome, this hypothesis has not been formally tested. To address these issues, we examined guano (feces) and distal intestinal mucosa from 19 species of free-ranging bats from Lamanai, Belize, using 16S rRNA amplicon sequencing to compare microbial communities across sample types. We found that the diversity and composition of intestine and guano samples differed substantially. In addition, we conclude that signatures of host evolution are retained by studying gut microbiomes based on mucosal tissue samples, but not fecal samples. Conversely, fecal samples retained more signal of host diet than intestinal samples. These results suggest that fecal and intestinal sampling methods are not interchangeable, and that these two microbiotas record different information about the host from which they are isolated.