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Front. Microbiol. | doi: 10.3389/fmicb.2019.01721

Adhesive Bifidobacterium induced changes in cecal microbiome alleviated constipation in mice

Linlin Wang1, Cailing Chen1, Shumao Cui1, Yuan-kun Lee2,  Gang Wang1*,  Zhao Jianxin1,  Hao Zhang1 and  Wei Chen1
  • 1Jiangnan University, China
  • 2National University of Singapore, Singapore

Constipation, which seriously affects living quality of people, is a common gastrointestinal disease. The engagement of the intestinal flora in the development of symptoms of constipation has been frequently hypothesized. In this study, constipated mice induced by loperamide were used to investige the alleviation of constipation by bifidobacteria. Bifidobactria was sorted out according to their adhesive properties into two groups. One group combined multiple strains of Bifidobacterium with adhesion property (CMB1), the other combined multiple strains of Bifidobacterium without adhesion property (CMB2). It was found that CMB1 can alleviate constipation more efficiently by improving the water, proprionate and butyrate content in faeces, and overall gastrointestinal transit time. Meanwhile, from the perspective of faecal microbiota, CMB1 alleviated constipation mainly by increasing the relative abundances of genera (Bifidobacterium, Lactobacillus and Prevotella) associated with rapid bowel movement. From the perspective of cecal microbiota, CMB1 alleviated constipation mainly by increasing the relative abundances of genera Lactobacillus, Bacteroides, unclassified S24-7, Dorea, Ruminococcus, Coprococcus, and Rikenella, and decreasing the relative abundances of genera Oscillospira, Odoribacter and Unclassified F16, which are associated with methane production and colonic transit. Overall, changes of microbiota in caecum by CMB1 reflect the stage of constipation in mice more comprehensively than that in faeces.

Keywords: Bifidobacterium, Constipation, Adhesion properties, SCFAs, Gut Microbiota

Received: 01 Apr 2019; Accepted: 12 Jul 2019.

Edited by:

Jie YIN, Institute of Subtropical Agriculture (CAS), China

Reviewed by:

Atte Von Wright, University of Eastern Finland, Finland
Ana G. Binetti, CONICET Instituto de Lactología Industrial (INLAIN), Argentina
Jinping Chen, Guangdong Academy of Science (CAS), China  

Copyright: © 2019 Wang, Chen, Cui, Lee, Wang, Jianxin, Zhang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Gang Wang, Jiangnan University, Wuxi, 214122, Jiangsu Province, China, wanggang@jiangnan.edu.cn