Impact Factor 4.259 | CiteScore 4.30
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01954

A Comprehensive Antimicrobial Activity Evaluation of the Recombinant Antimicrobial Peptide Microcin J25 Against the Foodborne Pathogens Salmonella and E. coli O157 by using a matrix of conditions

 HaiTao Yu1, 2, Ning Li2, Xiangfang Zeng2,  Liu Lu2,  Yuming Wang2,  Gang Wang2,  Shuang Cai2,  Shuo Huang2,  Xiuliang Ding2, Qinglong Song2 and  Shiyan Qiao2*
  • 1China Agricultural University (CAU), China
  • 2State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, China

Natural microcin J25 (MccJ25) represent promising alternatives to traditional antibiotics for the treatment of drug-resistant infections. However, little is known about the antibacterial activity of recombinant MccJ25 against foodborne pathogens. Here, the activity of recombinant MccJ25 was examined using a matrix of conditions in order to assess the efficacy of recombinant MccJ25 as a mitigation against foodborne pathogens, Salmonella species and Escherichia coli (E. coli) O157. Results showed that recombinant MccJ25 displayed excellent antimicrobial activity against these foodborne pathogens, including clinical isolates of Salmonella and E. coli, as well as clinical antibiotic-resistant Salmonella and E. coli isolates with different minimal inhibitory concentrations. In addition, antimicrobial activity curves and live/dead assay evidenced that recombinant MccJ25 harbors strong bactericidal activity against salmonella and E. coli O157. Notably, recombinant MccJ25 also had great potency and induced fast mortality against different growth phase of Salmonella and E. coli. The stability analysis results showed that the activity of recombinant MccJ25 was not influenced by temperatures as high as 121°C. Varying the pH from 2.0 to 9.0 did not appear to affect the activity of recombinant MccJ25. Under the challenge of several proteases, simulated gastrointestinal fluids and serum, recombinant MccJ25 still maintained exceptionally strong antimicrobial activity. Significant reductions in S. Pullorum levels were also achieved in food biological environments, such as milk, egg and meat. Moreover, we demonstrated that recombinant MccJ25 appeared to act by inducing membrane breaks, thinning, and disintegration in the S. Pullorum cytoplasmic membrane. Taken together, these results indicated that recombinant MccJ25 could be an effective alternative for mitigating and prevention of Salmonella and E. coli infection in food, animal and agriculture applications.

Keywords: Salmonella, Escherichia coli O157, Recombinant Microcin J25, Antimicrobial activity, Biological environments, stability, mitigation

Received: 10 Feb 2019; Accepted: 08 Aug 2019.

Edited by:

Steven L. Cobb, Durham University, United Kingdom

Reviewed by:

César De La Fuente, Massachusetts Institute of Technology, United States
Shankar Thangamani, College of Veterinary Medicine, Midwestern University, United States  

Copyright: © 2019 Yu, Li, Zeng, Lu, Wang, Wang, Cai, Huang, Ding, Song and Qiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Shiyan Qiao, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China, qiaoshiyan@cau.edu.cn