CORRECTION article

Front. Microbiol., 04 May 2022

Sec. Evolutionary and Genomic Microbiology

Volume 13 - 2022 | https://doi.org/10.3389/fmicb.2022.924519

Corrigendum: Molecular Analysis of Bacterial Isolates From Necrotic Wheat Leaf Lesions Caused by Xanthomonas translucens, and Description of Three Putative Novel Species, Sphingomonas albertensis sp. nov., Pseudomonas triticumensis sp. nov. and Pseudomonas foliumensis sp. nov.

  • 1. Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada

  • 2. Crop Diversification Centre South, Alberta Agriculture and Forestry, Brooks, AB, Canada

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The naming of the two new Pseudomonas species as Pseudomonas triticumensis sp. nov. and Pseudomonas foliumensis sp. nov. was not in accordance with the rules of the International Code of Nomenclature of Prokaryotes (ICNP) and as such cannot be validly published. This corrigendum is prepared to change the previous names to Pseudomonas triticifolii sp. nov. and Pseudomonas folii sp. nov., respectively, based on ICNP rules.

Also, the stereoisomers of the carbon sources used are now correctly indicated with capital letters, e.g., D-melibiose, D-arabitol, D-glucose, L-malic acid … etc. instead of d-melibiose, d-arabitol, d-glucose, l-malic acid...

A correction has been made to the protologues of the two novel Pseudomonas species, Description ofPseudomonas triticumensis sp. nov. and Description ofPseudomonas foliumensissp. nov:

Description ofPseudomonas triticifoliisp. nov.

Pseudomonas triticifolii sp. nov. (tri.ti.ci.fo'li.i. L. neut. n. triticum, wheat; L. neut. n. folium, a leaf; N.L. gen. n. triticifolii, of a wheat leaf).

Cells are aerobic, Gram-reaction-negative, non-spore-forming rods (0.6–1.0 μm wide and 2.0–4.5 μm long), motile with one, or multiple polar flagella. After 48 h on KB, colonies are white-yellowish and circular (averae 2–4 mm, in diameter), convex with regular margins and produce fluorescent pigments. Growth in different NaCl concentrations optimal at 4%, and grows at 4°C with optimal growth at 28–30°C and no growth at 40°C. The major cellular fatty acid peaks of the bacteria were C16:0, C16:1 ω6c/C16:1 ω7c (summed feature 3), C18:1 ω7c/C18:1 ω6c (summed feature 8), C17:0 cyclo; C12:0 2-OH, C12:0 3-OH and C10:0 3-OH. Based on Biolog GENIII microplate assays, strain 32L3A utilized 37 carbon sources including D-melibiose, D-arabitol, α-D-glucose, stachyose, myo- inositol, D-mannitol, D-sorbitol, D-cellobiose, gentiobiose, L-serine but not L-lactic acid, citric acid, α-keto-glutaric acid, D-malic acid, L-malic acid, α-keto-butyric acid, acetoacetic acid, propionic acid, acetic acid, D-glucuronic acid, glucuronamide, mucic acid, quinic acid, D-saccharic acid. Using API ZYM assays, these bacteria are positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, and β-glucosidase, but negative for α-glucosidase, α-galactosidases, β-galactosidases and β-glucuronidases. The most abundant fatty acids are C16:0, C16:1ω7c and/or C16:1ω6c (summed feature 3) and C18:1ω7c and/or C18:1ω7c (summed feature 8). These bacteria are resistant to troleandomycin, rifamycin SV, vancomycin, lincomycin but sensitive to nalidixic acid and minocycline. The type strain is 32L3AT (= DOAB 1067T = CECT 30249T = LMG 32140T), isolated from necrotic wheat leaf tissues naturally infected by Xanthomonas translucens from Alberta, Canada. The DNA G + C content of type strain 32L3AT is 59.3 %.

Description ofPseudomonas foliisp. nov.

Pseudomonas folii sp. nov. (fo'li.i. L. gen. n. folii, of a leaf).

Cells are aerobic, Gram-reaction-negative, non-spore-forming rods (0.6–1.0 μm wide and 1.5–2.5 μm long), motile with one or two polar flagella. After 48 h on KB, colonies are white-yellowish and circular (average 4 mm, in diameter), convex with regular margins and do not produce fluorescent pigments. Growth in NaCl concentrations optimal at 4%, and grows at 4°C with optimal growth at 28–30°C and no growth at 40°C. Strong growth at pH 6 but very weak growth at pH 5. The major cellular fatty acid peaks of the Pseudomonas strains were C16:0, C16:1 ω6c/C16:1 ω7c (summed feature 3), C18:1 ω7c/C18:1 ω6c (summed feature 8), C17:0 cyclo; C12:0 2-OH, C12:0 3-OH and C10:0 3-OH. Based on Biolog GENIII microplate assays, these bacteria readily utilize 47 carbon sources e.g., α-D-glucose, D-mannose, gelatin, pectin, D-fructose, D-galactose, 3-methyl glucose, D-fucose, L-rhamnose, D-raffinose, D-fucose, α-D-Lactose, D-melibiose, β-methyl-D-glucoside, D-salicin, dextrin but not gentiobiose, sucrose, L-aspartic acid, L-glutamic acid, L-histidine, D-glucuronic acid, glucuronamide, mucic acid, quinic acid, citric acid, α-keto-glutaric acid, D-malic acid and L-malic acid. Using API ZYM assays, these bacteria are positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, and β-glucosidase, but negative for α-glucosidase, α-galactosidases, β-galactosidases and β-glucuronidases. The most abundant fatty acids are C16:0, C16:1ω7c and/or C16:1ω6c (summed feature 3) and C18:1ω7c and/or C18:1ω7c (summed feature 8). These bacteria are resistant to troleandomycin, rifamycin SV, vancomycin, lincomycin but sensitive to nalidixic acid and minocycline. The type strain is 10L4BT (= DOAB 1069T = CECT 30250T = LMG 32142T), isolated from necrotic wheat leaf tissues naturally infected by Xanthomonas translucens from Alberta, Canada. The DNA G + C content of the type strain 10L4BT is 57.2%.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.

Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Summary

Keywords

wheat bacterial leaf streak disease, Xanthomonas translucens, cultivable bacteria, Genome-based DNA-DNA hybridization (gDDH), Average Nucleotide Identity (ANI), Multilocus sequence analysis (MLSA), MALDI-TOF

Citation

Tambong JT, Xu R, Gerdis S, Daniels GC, Chabot D, Hubbard K and Harding MW (2022) Corrigendum: Molecular Analysis of Bacterial Isolates From Necrotic Wheat Leaf Lesions Caused by Xanthomonas translucens, and Description of Three Putative Novel Species, Sphingomonas albertensis sp. nov., Pseudomonas triticumensis sp. nov. and Pseudomonas foliumensis sp. nov.. Front. Microbiol. 13:924519. doi: 10.3389/fmicb.2022.924519

Received

20 April 2022

Accepted

21 April 2022

Published

04 May 2022

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Frontiers Editorial Office, Frontiers Media SA, Switzerland

Volume

13 - 2022

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Copyright

*Correspondence: James T. Tambong

This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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