Original Research ARTICLE
Hyperoxygenation as a therapeutic supplement for treatment of triple negative breast cancer
- 1Geisel School of Medicine, Dartmouth College, United States
Triple-negative breast cancer (TNBC) refers to a group of biologically aggressive breast cancers that do not express estrogen, progesterone or epidermal growth factor receptor 2 hormone receptors. Each subset of TNBC has a unique molecular profile and may require specific treatments. A combination of surgery and chemotherapy followed by radiation therapy is the standard treatment mode for TNBC patients. Tumor oxygen status (hypoxia) is a key factor that may compromise the effectiveness of radiation treatment, as it is known that hypoxia can confer radiation resistance. In this study, we characterized MDA-MB-231 orthotropic xenograft tumors with respect to tumor oxygen level and their response to supplemental oxygen therapy in combination with paclitaxel and radiation therapy. We observed that the TNBC tumors became severely hypoxic (pO2 < 4 mmHg) within 1 week of tumor growth and responded poorly to administration of respiratory hyperoxygenation (100% O2) to mitigate hypoxia. However, periodic administration of supplemental oxygen (100% O2; 60 min/day for 21 days) showed a significant inhibitory effect on tumor volume when compared to control (102332 mm3 versus 1378114 mm3; p<0.05). Combination of supplemental oxygen with paclitaxel and radiation therapy led to a significant reduction in tumor growth when compared to radiation alone (23940 mm3 versus 39032 mm3; p<0.05). The therapeutic enhancement by supplemental oxygen is possibly attributed to increase in tumor oxygenation with paclitaxel at the time of radiation treatment. These findings may have important implications in the understanding the role of oxygen and supplemental oxygen therapy for the treatment of TNBC patients.
Keywords: Triple negative breast cancer, tumor hypoxia, EPR oximetry, Paclitaxel, Radiation, Supplemental oxygen
Received: 03 Sep 2018;
Accepted: 26 Oct 2018.
Edited by:William Small, Jr., Stritch School of Medicine, United States
Reviewed by:Valdir C. Colussi, University Hospitals Cleveland Medical Center, United States
Ima Paydar, Hospital of the University of Pennsylvania, United States
Copyright: © 2018 Kuppusamy and Mast. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Periannan Kuppusamy, Geisel School of Medicine, Dartmouth College, Hanover, United States, kuppu@Dartmouth.edu