Original Research ARTICLE
Allogenic adipose derived stem cells transplantation improved sciatic nerve regeneration in rats: Autologous nerve graft model.
- 1OpenLab «Gene and Cell Technologies», Kazan Federal University, Russia
- 2Republican Clinical Hospital, Russia
- 3Kazan State Medical University, Russia
- 4Bauman Kazan State Academy of Veterinary Medicine, Russia
We examined the effect of transplantation of allogenic adipose-derived stem cells (ADSCs) with properties of mesenchymal stem cells (MSCs) on posttraumatic sciatic nerve regeneration in rats. We suggested an approach to rat sciatic nerve reconstruction using the nerve from the other leg as a graft. The comparison was that of a critical 10 mm nerve defect repaired by means of autologous nerve grafting versus an identical lesion on the contralateral side. In this experimental model, the same animal acts simultaneously as a test model, and control. Regeneration of the left nerve was enhanced by the use of ADSCs, whereas the right nerve healed under natural conditions. Thus the effects of individual differences were excluded and a result closer to clinical practice obtained. We observed significant destructive changes in the sciatic nerve tissue after surgery which resulted in the formation of combined contractures in knee and ankle joints of both limbs and neurotrophic ulcers only on the right limb. The stimulation of regeneration by ADSCs increased the survival of spinal L5 ganglia neurons by 26.4%, improved sciatic nerve vascularization by 35.68% and increased the number of myelin fibers in the distal nerve by 41.87%. Moreover, we have demonstrated that S100, PMP2 and PMP22 gene expression levels are suppressed in response to trauma as compared to intact animals. We have shown that ADSC-based therapy contributes to significant improvement in the regeneration
Keywords: PNI, autologous nerve graft, DRG, PCR, Myelin fibers, IVIS spectrum
Received: 12 Jul 2017;
Accepted: 25 Jan 2018.
Edited by:Aaron M. Mohs, University of Nebraska Medical Center, United States
Reviewed by:Hong Zhan, University of Wisconsin-Madison, United States
Justin M. Saul, Miami University, United States
Copyright: © 2018 Masgutov, Masgutova, Mukhametova, Garanina, Arkhipova, Zakirova, Mukhamedshina, Gilazieva, Syromiatnikova, Mullakhmetova, Kadyrova, Nigmetzyanova, Mikhail, Yagudin and Rizvanov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Albert Rizvanov, Kazan Federal University, OpenLab «Gene and Cell Technologies», Kazan, Russia, firstname.lastname@example.org