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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2018.01047

Chelerythrine attenuates the inflammation of lipopolysaccharide -induced acute lung inflammation through NF-κB signaling Pathway mediated by Nrf2

 Lu Fan1, 2,  Ye Fan3,  Li Liu4,  Wei W. Tao2,  Xin Shan2, Yu Dong2, Lin Li2,  Sen Zhang2 and  Han Q. Wang1, 2, 5*
  • 1School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, China
  • 2School of Basic Biomedical Science, Nanjing University of Chinese Medicine, China
  • 3Department of Emergency Medicine, Nanjing General Hospital of Nanjing Military Command, China
  • 4School of Pharmacy, Guangdong Medical University, China
  • 5College of Pharmacy, Ningxia Medical University, China

Chelerythrine (CH), is a kind of benzo[c] phenanthridine alkaloid isolated from plants such as Chelidonium, with pharmacological activities as antitumor, antibiosis and anti-inflammation. However, few studies have demonstrated whether CH could protect against lipopolysaccharide (LPS)-induced acute lung injury (ALI), and the underlying mechanism is also uncertain. The purpose of the present study was to investigate the anti-inflammatory effects of CH on LPS-induced ALI in mice and in RAW264.7 cells. In this study, we demonstrated that treatment with CH significantly ameliorated LPS-induced pathological changes in the lung. CH also attenuated LPS-induced W/D ratio, inflammatory cell infiltration. Meanwhile, LPS-induced Tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6) and interleukin 1β (IL-1β) production and oxidative stress were markedly suppressed by CH. Furthermore, western blot showed that CH suppressed LPS-stimulated inflammation of RAW264.7 cells through activation of nuclear factor kappa-B (NF-κB) pathway. Knocking down of nuclear factor erythroid 2-related factor 2 (Nrf2) led to the reduction of nuclear translocation of the NF-κB p65, which triggers inflammation. These experimental results provided evidence that CH could be a potential therapeutic candidate for the intervention of ALI caused by LPS.

Keywords: Chelerythrine, Inflammation, LPS, Ali, NF-κB, Nrf2

Received: 11 Apr 2018; Accepted: 30 Aug 2018.

Edited by:

Suresh Kumar, Putra Malaysia University, Malaysia

Reviewed by:

Rajasekaran Subbiah, Bharathidasan Institute of Technology Campus, University College of Engineering, Anna University, India
Luigi Menghini, Dipartimento di Farmacia, Università 'G. d'Annunzio' di Chieti-Pescara, Italy  

Copyright: © 2018 Fan, Fan, Liu, Tao, Shan, Dong, Li, Zhang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: PhD. Han Q. Wang, Nanjing University of Chinese Medicine, School of Medicine and Life Sciences, Nanjing, China, hqwangnx@tom.com