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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2018.01486

Gallic acid potentiates the antimicrobial activity of tulathromycin against two key bovine respiratory disease (BRD) causing-pathogens

  • 1University of Saskatchewan, Canada

Bovine respiratory disease (BRD) is the most common infectious disease in dairy and beef cattle. It is associated with significant morbidity and mortality and causes a huge economic loss each year. In western Canada, a one-time injection of tulathromycin is commonly used as a metaphylactic procedure to reduce BRD incidence and eliminate potential BRD outbreak. With increased global concern on antibiotic usage in dairy and beef products and bacterial resistance to antibiotics, it is important to develop a novel strategy to eliminate the usage or decrease the dosage of antibiotics. In this study, we showed that gallic acid was active against both Mannheimia haemolytica and Pasteurella multocida, two key BRD causing-pathogens, with the minimum inhibitory concentration (MIC) measured at 250 g/mL and 500 g/mL, respectively. Co-administration of tulathromycin and gallic acid exhibited a strong additive or weak synergistic effect towards both M. haemolytic and P. multocida. Tulathromycin, gallic acid and their combination were also effective against the co-culture of M. haemolytic and P. multocida. Furthermore, we showed that pre-exposure to tulathromycin generated bacterial resistance to the antibiotic in M. haemolytica but not in P. multocida.

Keywords: bovine respiratory disease, Gallic Acid, tulathromycin, Dairy and beef cattle, antimicrobial resistance

Received: 20 Jul 2018; Accepted: 04 Dec 2018.

Edited by:

Banasri Hazra, Jadavpur University, India

Reviewed by:

Timothy J. Mahony, The University of Queensland, Australia
Diganta Dey, Ashoke Laboratory Clinical Testing Centre Private Limited, India  

Copyright: © 2018 Rajamanickam, Yang and Sakharkar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Jian Yang, University of Saskatchewan, Saskatoon, S7N 5A2, Saskatchewan, Canada, jian.yang@usask.ca
Dr. Meena K. Sakharkar, University of Saskatchewan, Saskatoon, S7N 5A2, Saskatchewan, Canada, meena.sakharkar@usask.ca