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Front. Pharmacol. | doi: 10.3389/fphar.2018.01497

Correlation between C-MYC, BCL-2, and BCL-6 protein expression and gene trans-location as biomarkers in diagnosis and prognosis of diffuse large B-cell lymphoma

 YunXiang Zhang1*, Hui Wang1, Cuiai Ren1, Hai Yu1,  Wenjia Fang2, Na Zhang1, Sumei Gao1 and Qian Hou1
  • 1Weifang People's Hospital, China
  • 2Nanchang University, China

This study investigates the protein expression of C-MYC, BCL-2 and BCL-6 in diffuse large B-cell lymphoma (DLBCL) and their relationship with genetic abnormalities. A retro-spective study of 42 cases on paraffin-embedded tissue specimens diagnosed with DLBCL was performed using immunohistochemistry (IHC) and fluorescence in situ hy-bridization (FISH). The expression of C-MYC, BCL-2, BCL-6 protein and gene abnormali-ties in these tissue samples was analyzed. The relationship in genetic abnormalities and Ki-67, Hans classification, gender and age was also evaluated. It was found that the pos-itive rate of C-MYC expression was 47.6% (20/42), the rate of C-MYC gene abnormality was 26.2% (11/42), in which gene translocation accounted for 23.8% (10/42) and gene amplification 2.4% (1/42); C-MYC protein expression was positively correlated with C-MYC gene translocation (χ2=11.813; P=0.001); C-MYC gene translocation was mainly found in germinal center B cell type (χ2=4.029; P=0.045). The positive rate of BCL-2 pro-tein expression was 85.71% (36/42), the positive rate of translocation was 42.86% (18/42) and the amplification rate was 26.19% (11/42); the overexpression of BCL-2 protein was correlated with the BCL-2 translocation (χ2=3.407; P=0.029). The positive rate of BCL-6 protein expression was 45.24% (19/42), the positive rate of BCL-6 translocation was 14.29% (6/42) and the positive rate of BCL-6 amplification was 7.14% (3/42); the overex-pression of BCL-6 protein was significantly correlated with BCL-6 translocation (χ2=6.091; P=0.014). The Ki-67 index was significantly higher in C-MYC translocation cases than in non-C-MYC translocation cases (χ2=4.492; P=0.034). Taken together, our results suggest that the protein expression of C-MYC, BCL-2 and BCL-6 are positively correlated with their gene translocation. Overexpression of C-MYC, BCL-2, BCL- 6 pro-tein suggests the possibility of translocation. Therefore, immunohistochemical detection of C-MYC, BCL-2 and BCL-6 are useful in diagnosis and prognosis of DLBCL.

Keywords: Diffuse Lange B-cell Lymphoma, c-Myc, Bcl-2, Bcl-6, Gene translocation

Received: 05 Nov 2018; Accepted: 07 Dec 2018.

Edited by:

Dong-Hua Yang, St. John's University, United States

Reviewed by:

Ru Li, Stony Brook University, United States
Xiaozhuo Liu, University at Buffalo, United States  

Copyright: © 2018 Zhang, Wang, Ren, Yu, Fang, Zhang, Gao and Hou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. YunXiang Zhang, Weifang People's Hospital, Weifang, China,