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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00640

Mass spectrometry-based proteomics approach characterizes the dual functionality of miR-328 in monocytes

 Meike J. Saul1*,  Anett B. Hegewald1, Anne C. Emmerich2,  Elena Ossipova3, Mark Vogel1, Isabell Baumann1,  Kim Kultima4, Johan Lengqivst3,  Dieter Steinhilber5 and  Per-Johan Jakobsson3
  • 1Department of Biology, Darmstadt University of Technology, Germany
  • 2Institute of Pharmaceutical Chemistry, Goethe University Frankfurt am Main, Germany
  • 3Karolinska Institute (KI), Sweden
  • 4Uppsala University, Sweden
  • 5Goethe-Universität Frankfurt am Main, Germany

MicroRNAs (miRs) are small non-coding RNAs, which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR function. The recent discovery that miR-328 has a non-canonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such non-canonical miR function is not yet known. In order to achieve a better understanding of the non-canonical miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of non-canonical miR function.

Keywords: miR-328, Proteomics, TLR2, NOX2, p53, Inflammation, non-canonical miR function

Received: 21 Jan 2019; Accepted: 17 May 2019.

Edited by:

Orina Belton, University College Dublin, Ireland

Reviewed by:

Annalisa Trenti, University of Padova, Italy
Simone Marcone, Systems Biology Ireland, University College Dublin, Ireland  

Copyright: © 2019 Saul, Hegewald, Emmerich, Ossipova, Vogel, Baumann, Kultima, Lengqivst, Steinhilber and Jakobsson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Meike J. Saul, Department of Biology, Darmstadt University of Technology, Darmstadt, Germany, saul@bio.tu-darmstadt.de