Original Research ARTICLE
Dissecting the metabolic phenotype of similar antihypertensive effect with different fingerprints of five different Uncaria species on spontaneously hypertensive rats
- 1Shanghai Institute of Materia Medica (CAS), China
The sourcing of plants from multiple botanical origins is a common phenomenon in traditional Chinese medicines. Uncaria Stem with Hooks (UHs) is approved for using five botanical origins in the Chinese Pharmacopoeia (2015 Edition). All five UHs are commonly used for treating hypertension, even though the plants have different chromatographic fingerprints based on our previous study. However, their hypotensive effects and metabolic phenotypes have not been fully studied. In the present study, spontaneously hypertensive rats (SHRs) were orally administered five aqueous extracts (4 g crude drug/kg) prepared from the different UHs over a six-week period. Systolic blood pressure (SBP) was measured every week and urine was collected after SBP measurement. Untargeted metabonomics was performed using UPLC coupled with a LTQ-Orbitrap mass spectrometer. Bidirectional orthogonal projection to latent structures-discriminant analysis (O2PLS-DA), Student’s t test, and correlation analysis were used for pattern recognition and the selection of significant metabolites. A similar and prolonged reduction of SBP was observed in each of the groups given the five different UHs, while the metabolic profiles were perturbed slightly compared with that of SHR. Further analysis has shown that only a few common, different components were observed within the five groups which showed the similar antihypertensive effect in spite of the distinct metabolic pathways due to their different alkaloid composition. These results help understanding the mechanisms of the phenomenon “different species, same effect” of UHs.
Keywords: Uncaria Stem with Hooks, spontaneously hypertensive rats, systolic blood pressure, Metabonomics, Alkaloids, multiple botanical origins
Received: 28 Feb 2019;
Accepted: 02 Jul 2019.
Edited by:Haitao Lu, Shanghai Jiao Tong University, China
Reviewed by:XY Zhang, Centre for Molecular and Environmental Biology, University of Minho, Portugal
Andrei Mocan, Iuliu Hațieganu University of Medicine and Pharmacy, Romania
Copyright: © 2019 Wu, Feng, Hou, yu, Wu, Deng, Wang, zhi, zhang and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Prof. Wanying Wu, Shanghai Institute of Materia Medica (CAS), Shanghai, China, email@example.com
Prof. De-an Guo, Shanghai Institute of Materia Medica (CAS), Shanghai, China, firstname.lastname@example.org