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Front. Pharmacol. | doi: 10.3389/fphar.2019.00936

Five Active Components Compatibility of Astragali Radix and Angelicae Sinensis Radix Protect Hematopoietic Function Against Cyclophosphamide -Induced Injury in Mice and t-BHP–Induced Injury in HSCs

 Wei Zhang1, Jiahuan Zhu1, Hao Xu1, Xiaodan Liu1, Xiaoping Huang1 and Changqing Deng1*
  • 1Hunan University of Chinese Medicine, China

Although the compatibility of Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) has favorable effect on promoting hematopoiesis in traditional Chinese medicine (TCM), the main active components and pharmacological mechanism are unknown. We investigated the five active components and its mechanisms in vitro and in vivo. Five active components of Astragalus glycosides (AST), Formononetin (FRM), Ferulic acid (FRA), Calycosin (CAL) and Calycosin-7-glucoside (CLG), which could be absorbed in intestinal tract were detected in this study. The peripheral blood, HGF and HPCs colony were observed to evaluate the effect of these five active components promoting hematopoiesis. Furthermore, the HSCs proliferation, aging, cycle and related proteins were detected to explore the mechanism of these five components promoting HSCs proliferation. (i) The in vivo experiments showed that the combination of the five active components could remarkably increase the number of RBCs, WBCs, PLTs and content of Hb in peripheral blood and the area of bone marrow hematopoietic tissue, as well as the EPO, TPO, GM-CSF, colony of CFU-GM, CFU-MK, CFU-E, and BFU-E in serum. Each of these five components promoted the recovery of RBCs and Hb, and increased TPO, CFU-MK and CFU-E. All components except for AST increased the CFU-GM. FRA increased the number of WBCs, the area of bone marrow hematopoietic tissue and BFU-E. FRA and AST promoted PLTs recovery. FRA and CAL improved the content of GM-CSF. FRA, CAL and CLG improved the content of EPO. (ii) The in vitro experiments showed that FRA, FRM and AST significantly promoted cell proliferation, reduced the positive rate and G0/G1 cells, and increased G2/M+S cells and the expression of cyclin D1 and CDK4 proteins in aging HSCs. Furthermore, the combination of five components had the best effect. Taken together, the five active components of AST, FRM, FRA, CAL and CLG were the main pharmacodynamic substances of the AR-ASR compatibility, which promoted hematopoiesis. The combination of them had a synergistic effect. The mechanism of promoting hematopoiesisis may be relevant to regulating cyclin-related proteins, promoting cell cycle transformation, and promoting HSCs proliferation.

Keywords: Astragali radix (AR), Angelicae Sinensis Radix, hemopoiesis, Hematopoietic stem sell, cyclin

Received: 01 Apr 2019; Accepted: 22 Jul 2019.

Edited by:

Marcello Locatelli, Università degli Studi G. d'Annunzio Chieti e Pescara, Italy

Reviewed by:

Karl Tsim, Hong Kong University of Science and Technology, Hong Kong
WENCHUAN BI, Health Science Center, Shenzhen University, China  

Copyright: © 2019 Zhang, Zhu, Xu, Liu, Huang and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Changqing Deng, Hunan University of Chinese Medicine, Changsha, China, dchangq@sohu.com