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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00959

Antiviral properties of R. tanguticum nanoparticles on herpes simplex virus type I in vitro and in vivo

 Meng-xin Shen1, Nian Ma1, Min-ke Li1, Yuan-yuan Liu1, Tian Chen1, Fei Wei1, Dong-ying Liu1,  Wei Hou1, Hai-rong Xiong1* and  Zhan-qiu Yang1*
  • 1School of Basic Medical Sciences, State Key Laboratory of Virology, Wuhan University, China

Herpes simplex virus type 1 (HSV-1), an enveloped DNA virus, plays a key role in varieties of diseases including recurrent cold sores, keratoconjunctivitis, genital herpes and encephalitis in humans. Great efforts have been made in developing more effective and less side-effects anti-herpes simplex virus agents, including traditional Chinese herbal medicines. In the present study, we evaluated the antiviral efficacy of Rheum tanguticum nanoparticles against HSV-1 in vitro and in vivo. R. tanguticum nanoparticles could able to inactivate the HSV-1 virions and block the viral attachment and entry into cells. Time-of-addition assay indicated that R. tanguticum nanoparticles could interfere with the entire phase of viral replication. Besides, R. tanguticum nanoparticles showed the ability to inhibit the mRNA expression of HSV-1 immediate early gene ICP4 and early gene ICP8 as well as the expression of viral protein ICP4 and ICP8. Moreover, R. tanguticum nanoparticles have been proved to protect mice against HSV-1 induced lethality by decreasing the viral load and alleviated pathological changes in brain tissues. In conclusion, we demonstrated that R. tanguticum nanoparticles could inhibit HSV-1 infection through multiple mechanisms. These results suggest that R. tanguticum nanoparticles may have novel roles in the treatment of HSV-1 infection.

Keywords: R.tanguticum nanoparticles, HSV-1, antiviral, mouse model, viral protein

Received: 22 Mar 2019; Accepted: 29 Jul 2019.

Copyright: © 2019 Shen, Ma, Li, Liu, Chen, Wei, Liu, Hou, Xiong and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Hai-rong Xiong, State Key Laboratory of Virology, Wuhan University, School of Basic Medical Sciences, Wuhan, Hubei Province, China, hrxiong@whu.edu.cn
Prof. Zhan-qiu Yang, State Key Laboratory of Virology, Wuhan University, School of Basic Medical Sciences, Wuhan, Hubei Province, China, zqyang@whu.edu.cn