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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00986

Transcriptional Analysis of the Effects of Gambogic acid and Neogambogic acid: Inhibition of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

 Xin Hua1, Qin Yang1,  Wanjiang zhang1, Yue Jia1, Zhimin Dong2 and Siguo Liu1*
  • 1Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China
  • 2Tianjin Animal Husbandry and Veterinary Institute, Tianjin Agricultural University, China

Methicillin-resistant Staphylococcus aureus (MRSA) infection is a major threat to human health, as this bacterium has developed resistance to a variety of conventional antibiotics. This is especially true of MRSA biofilms, which not only exhibit enhanced pathogenicity and but also are resistant to most antibiotics. In this work, we demonstrated that two natural products with antitumor activity, namely, gambogic acid (GA) and neogambogic acid (NGA), have significant inhibitory activity toward MRSA. GA and NGA can not only effectively inhibit planktonic MRSA strains in vivo and in vitro but also have strong inhibitory effects on MRSA biofilms formation. By transcriptome sequencing, Q-RT-PCR and PRM, we found that GA and NGA could reduce the expression of S. aureus virulence factors by inhibiting the saeRS two-component system of MRSA, thus achieving inhibition. We found that GA and NGA had anti-MRSA activity in vivo and in vitro and identified saeRS to be the target, indicating that saeRS inhibitors may be used to treat biofilm-related infections.

Keywords: MRSA - Methicillin-resistant Staphylococcus aureus, SaeRS, Biofilm, Gambogic acid, Neogambogic acid

Received: 23 May 2019; Accepted: 31 Jul 2019.

Edited by:

Yonggang Zhang, West China Hospital, Sichuan University, China

Reviewed by:

Christian Agyare, Kwame Nkrumah University of Science and Technology, Ghana
Surjeet Verma, University of Pretoria, South Africa  

Copyright: © 2019 Hua, Yang, zhang, Jia, Dong and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Siguo Liu, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China,