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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.01098

Understanding the bacterial response to a mycotoxin: transcriptomic analysis of deoxynivalenol-induced changes in Devosia mutans 17-2-E-8

 Yousef I. Hassan1*, Ting Zhou1*, Dion Lepp1 and  Jian Wei He1
  • 1Agriculture and Agri-Food Canada (AAFC), Canada

Deoxynivalenol (DON) is a major fusarium toxin widely detected in cereal grains. The inadvertent exposure to this fungal secondary-metabolite gives rise to myriad adverse health effects including appetite-loss, emesis, and suppression of the immune system. While most of the attention to this mycotoxin has gained in the past four decades is related to its eukaryotic toxicity (monogastric-animals and plants more precisely), recent studies have begun to reveal its negative influence on prokaryotes. Recent evidence indicates that DON can negatively affect many bacterial species, raising the possibility of DON-induced imbalances within the microbiota of the human and animal gut, in addition to other environmental niches. This in turn has led to a greater interest in understanding bacterial responses towards DON, and the involved mechanisms and metabolic pathways, to build a more comprehensive picture of DON-induced changes in both prokaryotes and eukaryotes. This study reveals the transcriptomic profiling of Devosia mutans strain 17-2-E-8 after the inclusion of DON within its growth medium. The results highlight three adaptive mechanisms involved in the response of D. mutans 17-2-E-8 to this mycotoxin, which include: (a) ATP-binding cassette (ABC) transporters; (b) a toxin-specific pyrroloquinoline quinone (PQQ)-dependent detoxification pathway; and finally (c) the upregulation of auxiliary coping proteins such as porins, glutathione S-transferases, and phosphotransferase. Some of the identified mechanisms are universal in nature and shared with other bacterial genera and species.

Keywords: Deoxynivalenol, Devosia, RNA_Seq, prokaryotes, detoxification

Received: 31 Oct 2018; Accepted: 26 Aug 2019.

Copyright: © 2019 Hassan, Zhou, Lepp and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Yousef I. Hassan, Agriculture and Agri-Food Canada (AAFC), Ottawa, Canada, youhassan@yahoo.com
Dr. Ting Zhou, Agriculture and Agri-Food Canada (AAFC), Ottawa, Canada, ting.zhou@canada.ca