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Review ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.01166

In vitro use of cellular synthetic machinery for biosensing applications

 Dong-Myung Kim1* and Kyung-Ho Lee1
  • 1Chungnam National University, South Korea

The application of biosensors is expanding in diverse fields due to their high selectivity and sensitivity. Like other sensing devices, biosensors consist of two major parts, a recognition element and a transducer. In currently employed biosensors, the binding of the target analyte to a biological recognition element is generally transduced by electrochemical mechanisms. However, the amplifying nature of biosynthetic processes can potentially be harnessed to create fully biological sensing devices. Recent advances in the development of highly productive and cost-effective cell-free synthesis systems make it possible to use these systems as biological transducers to generate biosensing signals. This review surveys recent developments in cell-free biosensors, focusing on the newly devised mechanisms for the biological recognition of analytes to initiate the amplification processes of transcription and translation.

Keywords: Cell-free synthesis, bioanalysis, In vitro transcription, in vitro translation, biosensor, microbial biosensor

Received: 22 Feb 2019; Accepted: 10 Sep 2019.

Copyright: © 2019 Kim and Lee. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Dong-Myung Kim, Chungnam National University, Daejeon, South Korea, dmkim@cnu.ac.kr